Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived state of P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. P. vulgaris cells were procured from Micro BioLogics Inc., USA, in sealed pack bearing the American Type Culture Collection (ATCC 33420) number and stored according to the recommended storage protocol until needed for experiments. Lyophilized vial of ATCC strain of P. vulgaris were divided in two parts, Gr. I: control and Gr. II: treatment. Group II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10. Gr. IIB was stored and analysed on day 143. After retreatment on day 143, the sample was divided into three separate tubes. First, second and third tubes were analysed on day 5, 10 and 15 respectively. All experimental parameters were studied using automated Micro Scan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of P. vulgaris with other bacterial species after treatment. The antimicrobial susceptibility and minimum inhibitory concentration showed 10.71% and 15.63% alteration respectively in treated cells of P. vulgaris as compared to control. It was observed that few biochemical reactions (6%) were altered in the treated groups with respect to control. Moreover, biotype number was substantially changed in treated cells, Gr. IIA (62060406, Proteus penneri) on day 10 as compared to control (62070406; Proteus vulgaris). 16S rDNA analysis showed that the identified sample in this experiment was Proteus vulgaris after biofield treatment. However, the nearest homolog genus-species was found to be Proteus hauseri. The results suggested that biofield treatment has impact on P. vulgaris in lyophilized as well as revived state.
| Published in | International Journal of Genetics and Genomics (Volume 3, Issue 6) |
| DOI | 10.11648/j.ijgg.20150306.12 |
| Page(s) | 66-73 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Proteus vulgaris, Antimicrobial Susceptibility, Biofield Treatment, Biochemical Reaction, Biotype, 16S rDNA Analysis
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APA Style
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, et al. (2015). Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment. International Journal of Genetics and Genomics, 3(6), 66-73. https://doi.org/10.11648/j.ijgg.20150306.12
ACS Style
Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Sambhu Charan Mondal, et al. Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment. Int. J. Genet. Genomics 2015, 3(6), 66-73. doi: 10.11648/j.ijgg.20150306.12
AMA Style
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, et al. Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment. Int J Genet Genomics. 2015;3(6):66-73. doi: 10.11648/j.ijgg.20150306.12
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Download@article{10.11648/j.ijgg.20150306.12,
author = {Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Sambhu Charan Mondal and Snehasis Jana},
title = {Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment},
journal = {International Journal of Genetics and Genomics},
volume = {3},
number = {6},
pages = {66-73},
doi = {10.11648/j.ijgg.20150306.12},
url = {https://doi.org/10.11648/j.ijgg.20150306.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20150306.12},
abstract = {Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived state of P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. P. vulgaris cells were procured from Micro BioLogics Inc., USA, in sealed pack bearing the American Type Culture Collection (ATCC 33420) number and stored according to the recommended storage protocol until needed for experiments. Lyophilized vial of ATCC strain of P. vulgaris were divided in two parts, Gr. I: control and Gr. II: treatment. Group II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10. Gr. IIB was stored and analysed on day 143. After retreatment on day 143, the sample was divided into three separate tubes. First, second and third tubes were analysed on day 5, 10 and 15 respectively. All experimental parameters were studied using automated Micro Scan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of P. vulgaris with other bacterial species after treatment. The antimicrobial susceptibility and minimum inhibitory concentration showed 10.71% and 15.63% alteration respectively in treated cells of P. vulgaris as compared to control. It was observed that few biochemical reactions (6%) were altered in the treated groups with respect to control. Moreover, biotype number was substantially changed in treated cells, Gr. IIA (62060406, Proteus penneri) on day 10 as compared to control (62070406; Proteus vulgaris). 16S rDNA analysis showed that the identified sample in this experiment was Proteus vulgaris after biofield treatment. However, the nearest homolog genus-species was found to be Proteus hauseri. The results suggested that biofield treatment has impact on P. vulgaris in lyophilized as well as revived state.},
year = {2015}
}
TY - JOUR T1 - Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment AU - Mahendra Kumar Trivedi AU - Alice Branton AU - Dahryn Trivedi AU - Gopal Nayak AU - Sambhu Charan Mondal AU - Snehasis Jana Y1 - 2015/11/16 PY - 2015 N1 - https://doi.org/10.11648/j.ijgg.20150306.12 DO - 10.11648/j.ijgg.20150306.12 T2 - International Journal of Genetics and Genomics JF - International Journal of Genetics and Genomics JO - International Journal of Genetics and Genomics SP - 66 EP - 73 PB - Science Publishing Group SN - 2376-7359 UR - https://doi.org/10.11648/j.ijgg.20150306.12 AB - Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived state of P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. P. vulgaris cells were procured from Micro BioLogics Inc., USA, in sealed pack bearing the American Type Culture Collection (ATCC 33420) number and stored according to the recommended storage protocol until needed for experiments. Lyophilized vial of ATCC strain of P. vulgaris were divided in two parts, Gr. I: control and Gr. II: treatment. Group II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10. Gr. IIB was stored and analysed on day 143. After retreatment on day 143, the sample was divided into three separate tubes. First, second and third tubes were analysed on day 5, 10 and 15 respectively. All experimental parameters were studied using automated Micro Scan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of P. vulgaris with other bacterial species after treatment. The antimicrobial susceptibility and minimum inhibitory concentration showed 10.71% and 15.63% alteration respectively in treated cells of P. vulgaris as compared to control. It was observed that few biochemical reactions (6%) were altered in the treated groups with respect to control. Moreover, biotype number was substantially changed in treated cells, Gr. IIA (62060406, Proteus penneri) on day 10 as compared to control (62070406; Proteus vulgaris). 16S rDNA analysis showed that the identified sample in this experiment was Proteus vulgaris after biofield treatment. However, the nearest homolog genus-species was found to be Proteus hauseri. The results suggested that biofield treatment has impact on P. vulgaris in lyophilized as well as revived state. VL - 3 IS - 6 ER -
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