Background: Globally, respiratory diseases cause an estimated 1.9 million deaths per year. One of the most important aetiological organisms of both adult and childhood respiratory disease is Non-Typeable Haemophilus influenzae (NTHi). NTHi is frequently isolated from the respiratory tract during episodes of sinusitis, Otitis Media and pneumonia and is the most common cause of Chronic Obstructive Pulmonary Disease (COPD) and bronchiectasis exacerbations. Methods: The work has been effectively complemented with the compilation of the Database of Essential Genes (DEG) of H. influenzae Rd KW20. Each protein is subjected to BLASTP in NCBI server http://www.ncbi.nlm.nih.gov/blastp. The candidate drug targets are determined by KAAS (KEGG Automatic Annotation Server), KEGG ORTHOLOGY and KEGG GENES. Results: The given gram negative bacteria H. influenzae Rd KW20 has six distinguished domains i.e., cytoplasmic, cytoplasmic membrane, periplasmic, outer membrane, extracellular and unknown domains. Out of 642 essential genes, the predicted non-human Homologous are 412 in the different domain of given bacteria. With the help of KAAS (KEGG Automatic Annotation Server), KEGG ORTHOLOGY and KEGG GENES, we successfully identified 35 novel drug targets which have common metabolic pathways both in host and pathogen & pathogen specific metabolic pathways. Conclusion: The novel drug targets suggest those genes which are active in both the host and pathogen metabolic pathway and should be a pathogen specific metabolic pathway. The important drug target regions are vacJ, lepB, emrB, MurG & dgkA. vacJ is present in outer membrane while lepB, emrB, MurG&dgkA are present in cytoplasmic membrane. All these genes are fully sequenced and specifically annotated in KEGG PATHWAY.
| Published in | International Journal of Genetics and Genomics (Volume 2, Issue 4) |
| DOI | 10.11648/j.ijgg.20140204.13 |
| Page(s) | 62-67 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
NTHi (Non-Typeable H. Influenzae), NP (Non-Typeable) , DEG (Database of Essential Genes), Rd (Rough Derivative), ChoP (Phosphorylcholine), COPD (Chronic Obstructive Pulmonary Disease), SVM (Support Vector Machine), SCL (Subcellular Localization)
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APA Style
Ranjith Kumavath, Swaraj Prasad, Pratap Devarapalli, Debmalya Barh. (2014). In Silico Identification of Novel Candidate Drug Targets in Haemophilus Influenzae Rd KW20. International Journal of Genetics and Genomics, 2(4), 62-67. https://doi.org/10.11648/j.ijgg.20140204.13
ACS Style
Ranjith Kumavath; Swaraj Prasad; Pratap Devarapalli; Debmalya Barh. In Silico Identification of Novel Candidate Drug Targets in Haemophilus Influenzae Rd KW20. Int. J. Genet. Genomics 2014, 2(4), 62-67. doi: 10.11648/j.ijgg.20140204.13
AMA Style
Ranjith Kumavath, Swaraj Prasad, Pratap Devarapalli, Debmalya Barh. In Silico Identification of Novel Candidate Drug Targets in Haemophilus Influenzae Rd KW20. Int J Genet Genomics. 2014;2(4):62-67. doi: 10.11648/j.ijgg.20140204.13
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Download@article{10.11648/j.ijgg.20140204.13,
author = {Ranjith Kumavath and Swaraj Prasad and Pratap Devarapalli and Debmalya Barh},
title = {In Silico Identification of Novel Candidate Drug Targets in Haemophilus Influenzae Rd KW20},
journal = {International Journal of Genetics and Genomics},
volume = {2},
number = {4},
pages = {62-67},
doi = {10.11648/j.ijgg.20140204.13},
url = {https://doi.org/10.11648/j.ijgg.20140204.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20140204.13},
abstract = {Background: Globally, respiratory diseases cause an estimated 1.9 million deaths per year. One of the most important aetiological organisms of both adult and childhood respiratory disease is Non-Typeable Haemophilus influenzae (NTHi). NTHi is frequently isolated from the respiratory tract during episodes of sinusitis, Otitis Media and pneumonia and is the most common cause of Chronic Obstructive Pulmonary Disease (COPD) and bronchiectasis exacerbations. Methods: The work has been effectively complemented with the compilation of the Database of Essential Genes (DEG) of H. influenzae Rd KW20. Each protein is subjected to BLASTP in NCBI server http://www.ncbi.nlm.nih.gov/blastp. The candidate drug targets are determined by KAAS (KEGG Automatic Annotation Server), KEGG ORTHOLOGY and KEGG GENES. Results: The given gram negative bacteria H. influenzae Rd KW20 has six distinguished domains i.e., cytoplasmic, cytoplasmic membrane, periplasmic, outer membrane, extracellular and unknown domains. Out of 642 essential genes, the predicted non-human Homologous are 412 in the different domain of given bacteria. With the help of KAAS (KEGG Automatic Annotation Server), KEGG ORTHOLOGY and KEGG GENES, we successfully identified 35 novel drug targets which have common metabolic pathways both in host and pathogen & pathogen specific metabolic pathways. Conclusion: The novel drug targets suggest those genes which are active in both the host and pathogen metabolic pathway and should be a pathogen specific metabolic pathway. The important drug target regions are vacJ, lepB, emrB, MurG & dgkA. vacJ is present in outer membrane while lepB, emrB, MurG&dgkA are present in cytoplasmic membrane. All these genes are fully sequenced and specifically annotated in KEGG PATHWAY.},
year = {2014}
}
TY - JOUR T1 - In Silico Identification of Novel Candidate Drug Targets in Haemophilus Influenzae Rd KW20 AU - Ranjith Kumavath AU - Swaraj Prasad AU - Pratap Devarapalli AU - Debmalya Barh Y1 - 2014/08/20 PY - 2014 N1 - https://doi.org/10.11648/j.ijgg.20140204.13 DO - 10.11648/j.ijgg.20140204.13 T2 - International Journal of Genetics and Genomics JF - International Journal of Genetics and Genomics JO - International Journal of Genetics and Genomics SP - 62 EP - 67 PB - Science Publishing Group SN - 2376-7359 UR - https://doi.org/10.11648/j.ijgg.20140204.13 AB - Background: Globally, respiratory diseases cause an estimated 1.9 million deaths per year. One of the most important aetiological organisms of both adult and childhood respiratory disease is Non-Typeable Haemophilus influenzae (NTHi). NTHi is frequently isolated from the respiratory tract during episodes of sinusitis, Otitis Media and pneumonia and is the most common cause of Chronic Obstructive Pulmonary Disease (COPD) and bronchiectasis exacerbations. Methods: The work has been effectively complemented with the compilation of the Database of Essential Genes (DEG) of H. influenzae Rd KW20. Each protein is subjected to BLASTP in NCBI server http://www.ncbi.nlm.nih.gov/blastp. The candidate drug targets are determined by KAAS (KEGG Automatic Annotation Server), KEGG ORTHOLOGY and KEGG GENES. Results: The given gram negative bacteria H. influenzae Rd KW20 has six distinguished domains i.e., cytoplasmic, cytoplasmic membrane, periplasmic, outer membrane, extracellular and unknown domains. Out of 642 essential genes, the predicted non-human Homologous are 412 in the different domain of given bacteria. With the help of KAAS (KEGG Automatic Annotation Server), KEGG ORTHOLOGY and KEGG GENES, we successfully identified 35 novel drug targets which have common metabolic pathways both in host and pathogen & pathogen specific metabolic pathways. Conclusion: The novel drug targets suggest those genes which are active in both the host and pathogen metabolic pathway and should be a pathogen specific metabolic pathway. The important drug target regions are vacJ, lepB, emrB, MurG & dgkA. vacJ is present in outer membrane while lepB, emrB, MurG&dgkA are present in cytoplasmic membrane. All these genes are fully sequenced and specifically annotated in KEGG PATHWAY. VL - 2 IS - 4 ER -
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