Super-Ovulated Chinese Sika Deer (Cervus Nippon) and Assessment of Embryo Transfer at Developmental Stages

Zhao Jing; Madolo Nkosikhona; Lv Wenfa; M.J. Bringans; Wang Quankai

doi:doi:10.11648/j.ijgg.20140202.11

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Super-Ovulated Chinese Sika Deer (Cervus Nippon) and Assessment of Embryo Transfer at Developmental Stages

Zhao Jing, Madolo Nkosikhona, Lv Wenfa, M.J. Bringans, Wang Quankai

Published in International Journal of Genetics and Genomics (Volume 2, Issue 2)

Received: 2 March 2014 Accepted: 15 April 2014 Published: 30 April 2014

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Abstract

The Embryo transfer has been commercially recognized reproductive technique in the world. The study was conducted at a Jiutai local breeding deer farm in China. The breeding protocol was developed for embryo transfer in sika deer to be implemented at farm levels and under wild conditions. Success rates of the technique was measured through indicators like number of follicles developed as a result of a specific treatment protocol, super ovulation rates, and quality of embryos. The male sika deer (Cervus Nippon) were then selected in terms of semen quality, libido, and sperm productivity. Embryo recovery was performed by surgical technique, of which the percentage of the embryo developmental stages was measured, in terms of Blastocyst, Morula, Non-Fertilized. The Hinds (sample size=12) were synchronically induced by inserting a controlled internal drug release (CIDR)devices, injected with Follicle stimulating Hormone (FSH) i.m for 2-4days .The fertilization for hinds was achieved by natural mating after 4days after CIDR devices withdrawal .Embryo recovery was performed on Day 12(n=6) and Day13(n=6)of the breeding season. The major objective of this research was to evaluate the super- stimulation protocols and determine the frequency of recovered transferable embryo in sika deer. Nevertheless, there were no significant differences P<0.05 between the numbers of corpus luteum, Follicles, Blastocysts and Morula from both uterine horns. The embryo recovery showed positive outcomes of blastocyst, morula for sustainable genetic banking of pure sika deer.

Published in	International Journal of Genetics and Genomics (Volume 2, Issue 2)
DOI	10.11648/j.ijgg.20140202.11
Page(s)	13-18
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Embryo Transfer, Super Ovulation, Cryopreservation, Sika Deer

References

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APA Style

Zhao Jing, Madolo Nkosikhona, Lv Wenfa, M.J. Bringans, Wang Quankai. (2014). Super-Ovulated Chinese Sika Deer (Cervus Nippon) and Assessment of Embryo Transfer at Developmental Stages. International Journal of Genetics and Genomics, 2(2), 13-18. https://doi.org/10.11648/j.ijgg.20140202.11

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ACS Style

Zhao Jing; Madolo Nkosikhona; Lv Wenfa; M.J. Bringans; Wang Quankai. Super-Ovulated Chinese Sika Deer (Cervus Nippon) and Assessment of Embryo Transfer at Developmental Stages. Int. J. Genet. Genomics 2014, 2(2), 13-18. doi: 10.11648/j.ijgg.20140202.11

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AMA Style

Zhao Jing, Madolo Nkosikhona, Lv Wenfa, M.J. Bringans, Wang Quankai. Super-Ovulated Chinese Sika Deer (Cervus Nippon) and Assessment of Embryo Transfer at Developmental Stages. Int J Genet Genomics. 2014;2(2):13-18. doi: 10.11648/j.ijgg.20140202.11

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@article{10.11648/j.ijgg.20140202.11,
  author = {Zhao Jing and Madolo Nkosikhona and Lv Wenfa and M.J. Bringans and Wang Quankai},
  title = {Super-Ovulated Chinese Sika Deer (Cervus Nippon) and Assessment of Embryo Transfer at Developmental Stages},
  journal = {International Journal of Genetics and Genomics},
  volume = {2},
  number = {2},
  pages = {13-18},
  doi = {10.11648/j.ijgg.20140202.11},
  url = {https://doi.org/10.11648/j.ijgg.20140202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20140202.11},
  abstract = {The Embryo transfer has been commercially recognized reproductive technique in the world. The study was conducted at a Jiutai local breeding deer farm in China. The breeding protocol was developed for embryo transfer in sika deer to be implemented at farm levels and under wild conditions. Success rates of the technique was measured through indicators like number of follicles developed as a result of a specific treatment protocol, super ovulation rates, and quality of embryos. The male sika deer (Cervus Nippon) were then selected in terms of semen quality, libido, and sperm productivity. Embryo recovery was performed by surgical technique, of which the percentage of the embryo developmental stages was measured, in terms of Blastocyst, Morula, Non-Fertilized. The Hinds (sample size=12) were synchronically induced by inserting a controlled internal drug release (CIDR)devices, injected with Follicle stimulating Hormone (FSH) i.m for 2-4days .The fertilization for hinds was achieved by natural mating after 4days after CIDR devices withdrawal .Embryo recovery was performed  on Day 12(n=6) and Day13(n=6)of the breeding season. The major objective of this research was to evaluate the super- stimulation protocols and determine the frequency of recovered transferable embryo in sika deer. Nevertheless, there were no significant differences P<0.05 between the numbers of corpus luteum, Follicles, Blastocysts and Morula from both uterine horns. The embryo recovery showed positive outcomes of blastocyst, morula for sustainable genetic banking of pure sika deer.},
 year = {2014}
}

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TY  - JOUR
T1  - Super-Ovulated Chinese Sika Deer (Cervus Nippon) and Assessment of Embryo Transfer at Developmental Stages
AU  - Zhao Jing
AU  - Madolo Nkosikhona
AU  - Lv Wenfa
AU  - M.J. Bringans
AU  - Wang Quankai
Y1  - 2014/04/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ijgg.20140202.11
DO  - 10.11648/j.ijgg.20140202.11
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 13
EP  - 18
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20140202.11
AB  - The Embryo transfer has been commercially recognized reproductive technique in the world. The study was conducted at a Jiutai local breeding deer farm in China. The breeding protocol was developed for embryo transfer in sika deer to be implemented at farm levels and under wild conditions. Success rates of the technique was measured through indicators like number of follicles developed as a result of a specific treatment protocol, super ovulation rates, and quality of embryos. The male sika deer (Cervus Nippon) were then selected in terms of semen quality, libido, and sperm productivity. Embryo recovery was performed by surgical technique, of which the percentage of the embryo developmental stages was measured, in terms of Blastocyst, Morula, Non-Fertilized. The Hinds (sample size=12) were synchronically induced by inserting a controlled internal drug release (CIDR)devices, injected with Follicle stimulating Hormone (FSH) i.m for 2-4days .The fertilization for hinds was achieved by natural mating after 4days after CIDR devices withdrawal .Embryo recovery was performed  on Day 12(n=6) and Day13(n=6)of the breeding season. The major objective of this research was to evaluate the super- stimulation protocols and determine the frequency of recovered transferable embryo in sika deer. Nevertheless, there were no significant differences P<0.05 between the numbers of corpus luteum, Follicles, Blastocysts and Morula from both uterine horns. The embryo recovery showed positive outcomes of blastocyst, morula for sustainable genetic banking of pure sika deer.
VL  - 2
IS  - 2
ER  -

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Author Information

Zhao Jing

Jilin Agricultural University, Changchun, China
Madolo Nkosikhona

Jilin Agricultural University, Changchun, China
Lv Wenfa

Jilin Agricultural University, Changchun, China
M.J. Bringans

LIC- Advanced Genetics, Canada
Wang Quankai

Jilin Agricultural University, Changchun, China

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Zhao Jing, Madolo Nkosikhona, Lv Wenfa, M.J. Bringans, Wang Quankai. (2014). Super-Ovulated Chinese Sika Deer (Cervus Nippon) and Assessment of Embryo Transfer at Developmental Stages. International Journal of Genetics and Genomics, 2(2), 13-18. https://doi.org/10.11648/j.ijgg.20140202.11

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ACS Style

Zhao Jing; Madolo Nkosikhona; Lv Wenfa; M.J. Bringans; Wang Quankai. Super-Ovulated Chinese Sika Deer (Cervus Nippon) and Assessment of Embryo Transfer at Developmental Stages. Int. J. Genet. Genomics 2014, 2(2), 13-18. doi: 10.11648/j.ijgg.20140202.11

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AMA Style

Zhao Jing, Madolo Nkosikhona, Lv Wenfa, M.J. Bringans, Wang Quankai. Super-Ovulated Chinese Sika Deer (Cervus Nippon) and Assessment of Embryo Transfer at Developmental Stages. Int J Genet Genomics. 2014;2(2):13-18. doi: 10.11648/j.ijgg.20140202.11

Copy | Download

@article{10.11648/j.ijgg.20140202.11,
  author = {Zhao Jing and Madolo Nkosikhona and Lv Wenfa and M.J. Bringans and Wang Quankai},
  title = {Super-Ovulated Chinese Sika Deer (Cervus Nippon) and Assessment of Embryo Transfer at Developmental Stages},
  journal = {International Journal of Genetics and Genomics},
  volume = {2},
  number = {2},
  pages = {13-18},
  doi = {10.11648/j.ijgg.20140202.11},
  url = {https://doi.org/10.11648/j.ijgg.20140202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20140202.11},
  abstract = {The Embryo transfer has been commercially recognized reproductive technique in the world. The study was conducted at a Jiutai local breeding deer farm in China. The breeding protocol was developed for embryo transfer in sika deer to be implemented at farm levels and under wild conditions. Success rates of the technique was measured through indicators like number of follicles developed as a result of a specific treatment protocol, super ovulation rates, and quality of embryos. The male sika deer (Cervus Nippon) were then selected in terms of semen quality, libido, and sperm productivity. Embryo recovery was performed by surgical technique, of which the percentage of the embryo developmental stages was measured, in terms of Blastocyst, Morula, Non-Fertilized. The Hinds (sample size=12) were synchronically induced by inserting a controlled internal drug release (CIDR)devices, injected with Follicle stimulating Hormone (FSH) i.m for 2-4days .The fertilization for hinds was achieved by natural mating after 4days after CIDR devices withdrawal .Embryo recovery was performed  on Day 12(n=6) and Day13(n=6)of the breeding season. The major objective of this research was to evaluate the super- stimulation protocols and determine the frequency of recovered transferable embryo in sika deer. Nevertheless, there were no significant differences P<0.05 between the numbers of corpus luteum, Follicles, Blastocysts and Morula from both uterine horns. The embryo recovery showed positive outcomes of blastocyst, morula for sustainable genetic banking of pure sika deer.},
 year = {2014}
}

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TY  - JOUR
T1  - Super-Ovulated Chinese Sika Deer (Cervus Nippon) and Assessment of Embryo Transfer at Developmental Stages
AU  - Zhao Jing
AU  - Madolo Nkosikhona
AU  - Lv Wenfa
AU  - M.J. Bringans
AU  - Wang Quankai
Y1  - 2014/04/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ijgg.20140202.11
DO  - 10.11648/j.ijgg.20140202.11
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 13
EP  - 18
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20140202.11
AB  - The Embryo transfer has been commercially recognized reproductive technique in the world. The study was conducted at a Jiutai local breeding deer farm in China. The breeding protocol was developed for embryo transfer in sika deer to be implemented at farm levels and under wild conditions. Success rates of the technique was measured through indicators like number of follicles developed as a result of a specific treatment protocol, super ovulation rates, and quality of embryos. The male sika deer (Cervus Nippon) were then selected in terms of semen quality, libido, and sperm productivity. Embryo recovery was performed by surgical technique, of which the percentage of the embryo developmental stages was measured, in terms of Blastocyst, Morula, Non-Fertilized. The Hinds (sample size=12) were synchronically induced by inserting a controlled internal drug release (CIDR)devices, injected with Follicle stimulating Hormone (FSH) i.m for 2-4days .The fertilization for hinds was achieved by natural mating after 4days after CIDR devices withdrawal .Embryo recovery was performed  on Day 12(n=6) and Day13(n=6)of the breeding season. The major objective of this research was to evaluate the super- stimulation protocols and determine the frequency of recovered transferable embryo in sika deer. Nevertheless, there were no significant differences P<0.05 between the numbers of corpus luteum, Follicles, Blastocysts and Morula from both uterine horns. The embryo recovery showed positive outcomes of blastocyst, morula for sustainable genetic banking of pure sika deer.
VL  - 2
IS  - 2
ER  -

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