The root tubers of cassava (Manihot esculenta Crantz) contain varying amounts of cyanogenic glucosides which liberate toxic hydrogen cyanide with the resultant effect of poisoning especially the highly cyanogenic cultivars. These include some of the local and improved cassava varieties grown in Zombo district, Uganda among others. Therefore, this study explored the effectiveness of some traditional processing techniques used singly and in combination to reduce cyanide levels in highly toxic root tubers of selected local (Nyar-anderiano, Nyar-papoga, Nyar-udota, Nyar-pamitu, Bisimwenge, Nyar-matia) and improved (NASE 3, NASE 9, NASE 14, NASE 19, TME 14 and TME 204) cassava varieties grown in Zombo District, Uganda. The processed and the fresh cassava tubers were analysed for Hydrogen cyanide (HCN) by the standard titration method. The results indicated that heap fermentation followed by sun-drying reduced the cyanide levels in all the selected improved varieties to WHO safe levels for consumption (below 10mg/kg) in the dried chips. Mixed processing methods combining soaking, fermentation and sun-drying reduced the cyanide concentrations in the local varieties (Nyar-udota, Nyar-anderiano) and improved variety (NASE 19) to World Health Organisation (WHO) safe levels. None of the processing techniques used singly reduced the HCN levels in the root tubers of the selected cassava varieties to safe levels. Hence mixed strategies combining several traditional cassava processing techniques should be adopted to detoxify the selected cassava varieties of cyanogens. The local cassava varieties (Bisimwenge, Nya-matia, Nya-pamitu, Nya-papoga) would require modifications of the combined traditional processing techniques or other more complex processes to detoxify them of HCN before human consumption.
Published in | International Journal of Food Science and Biotechnology (Volume 2, Issue 4) |
DOI | 10.11648/j.ijfsb.20170204.14 |
Page(s) | 121-125 |
Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Detoxification, Food Safety, Hydrogen Cyanide, Traditional Methods
[1] | D. Nhassico, H. Muquingue, J. Cliff, A. Cumbana, and J. H. Bradbury, “Rising African cassava production, diseases due to high cyanide intake and control measures”. Journal of the Science of Food and Agriculture, 88, 2043–2049, 2008. |
[2] | R. J. Hillocks, “Cassava in Africa”. In: R. J. Hillocks, J. M. Thresh, and A. C. Bellotti, (eds). Cassava: Biology, Production and Utilization. CAB International publishers, Wallingford, UK (pp. 41-54), 2002. |
[3] | Uganda Bureau of Statistics, Zombo District Socio-Economic Report, Volume II. Subcounty Development Programme: Implementation of The Community Information System (CIS), Uganda Bureau of Statistics, Kampala, 2012. Retrieved from www.ubos.org. |
[4] | B. Adamolekun, “Neurological Disorders associated with Cyanogenic Glycosides in Cassava: A Review of Putative Etiologic Mechanisms”. In: C. M. Pace (ed.), Cassava: Farming, Uses, and Economic Impact. Nova Science Publishers, Inc. New York. pp. 165-180, 2012. |
[5] | V. Lebot, “Tropical root and tuber crops: cassava, sweet potato, yams, aroids”. CAB International Publishers. Oxfordshire, UK, 2009. |
[6] | F. Nartey, “Cassava–Cyanogenesis, Ultrastructure and Seed Germination”. Munksgaard, Copenhagen, 1978. |
[7] | P. C. R. Arguedas, “Residual cyanide concentrations during the extraction of cassava starch”. Food Technol. 17: 251-262, 1982. http://dx.doi.org/10.1111/j.1365-2621-1982.tb00180.x. |
[8] | D. Siritunga, and R. T. Sayre, “Generation of cyanogens-free transgenic cassava”. Planta, 217, 367-373, 2003. http://dx.doi.org/10.1007/s00425-003-1005-8. PMid: 14520563. |
[9] | A. Westby, “Cassava utilization, storage and small-scale processing”. In: R. J. Hillocks, J. M. Thresh, and A. C. Bellotti, (eds) Cassava, Biology, Production and Utilization. CAB International, Wallingford, UK, pp. 281–300, 2002. |
[10] | N. L. V. Mlingi, Z. A. Bainbridge, N. H. Poulter, and H. Rosling, “Critical stages in cyanogen removal during cassava processing in southern Tanzania”. Food Chem, 53, 29-33, 1995. |
[11] | E. M. Salvador, V. Steenkamp, and C. M. E. McCrindle, “Production, consumption and nutritional value of cassava (Manihot esculenta, Crantz) in Mozambique: An overview.” Journal of Agricultural Biotechnology and Sustainable Development, 6(3), 29-38, 2014. doi: 10.5897/JABSD2014.0224. |
[12] | R. D. Cooke, and E. Maduagwu, “The effects of simple processing on the cyanide content of cassava chips”. J Food Technol., 13, 299–306, 1978. |
[13] | B. O. Osuntokun, “Chronic cyanide intoxication of dietary origin and a degenerative neuropathy in Nigerians”. Acta Horticulturae, 375, 311–321, 1994. |
[14] | M. Ernesto, A. P. Cardoso, D. Nicala, E. Mirione, F. Massaza, J. Cliff, M. R. Haque, and J. H. Bradbury, “Persistent konzo and cyanogen toxicity from cassava in northern Mozambique”. Acta Tropica, 82, 357–362, 2002. |
[15] | F. Delange, L. O. Ekpechi, and H. Rosling, “Cassava cyanogenesis and iodine deficiency disorders”. Acta Hortic. 375, 289-293, 1994. |
[16] | N. Mlingi, N. H. Poulter, and H. Rosling, “An outbreak of acute intoxications from consumption of insufficiently processed cassava in Tanzania”. Nutrition Res., 12, 677-687, 1992. |
[17] | A. Akintonwa, O. Tunwashe, and A. Onifade, “Fatal and non-fatal acute poisoning attributed to cassava–based meal”. Acta Horticulturae, 375, 285–288, 1994. |
[18] | D. L. Dufour, “Bitter’ cassava: toxicity and detoxification”. In: R. Ortiz, and N. M. A. Nassar, (eds). Proceedings of the First International Meeting on Cassava Breeding, Biotechnology and Ecology. Universidade de Brazilia, Brazil, pp. 171–184, 2007. |
[19] | B. Nambisan, and S. Sundaresan, “Effect of Processing on the Cyanoglucoside Content of Cassava”. J. Sci. Food Agric., 36, 1197-1203, 1985. |
[20] | A. P. Cardoso, M. Ernesto, J. Cliff, S. V. Egan, and J. H. Bradbury, “Cyanogenic potential of cassava flour: field trial in Mozambique of a simple kit”. International Journal Food Science and Nutrition, 49, 93–99, 1998. |
[21] | M. Andama, and B. J. Lejju, “Potential of Fermentation in Detoxifying Toxic Cassava Root Tubers”. Journal of Agricultural Science and Technology A 2, 1182-1188, 2012. |
[22] | M. Andama, J. B. Lejju, and B. Oloya, “Potential of Soaking and Sun-Drying in Detoxifying Toxic Cassava Root Tubers”. International Journal of Food Science and Biotechnology, 2 (5), 103-105, 2017. doi: 10.11648/j.ijfsb.20170205.11. |
[23] | S. K. Hahn, “An overview of traditional processing and utilization of cassava in Africa”. n.d. http://www.fao.org/wairdocs/ilri/x5458e/x5458e05.htm#TopOfPage. Retrieved on 12th/07/2017. |
[24] | J. A. Montagnac, C. R. Davis, and S. A. Tanumihardjo, “Processing Techniques to Reduce Toxicity and Antinutrients of Cassava for Use as a Staple Food”. Comprehensive Reviews in Food Science and Food Safety, 8, 17-27, 2009. |
[25] | B. Oloya, C. Adaku, E. Ntambi, and M. Andama, “Cyanogenic Potential of Selected Cassava Varieties in Zombo District, Uganda”. International Journal of Nutrition and Food Sciences, 6 (3), 144-148, 2017. doi: 10.11648/j.ijnfs.20170603.16. |
[26] | FAO, “Processing and utilization of Root and Tuber Crops”. FIAT PANIS, Rome, 2000. |
[27] | FAO/WHO,“Joint FAO/WHO food standard programme”. Codex Alimentarius Commission XII, Supplement 4, Rome, Italy: FAO, pp. 1-42, 1991. |
[28] | A. J. A. Essers, “Making safe flour from bitter cassava by indigenous solid substrate fermentation”. Acta Hortic., 375, 217–24, 1994. |
[29] | M. Lambri, M. D. Fumi, A. Roda, and D. M. De Faveri, “Improved processing methods to reduce the total cyanide content of cassava roots from Burundi”. African Journal of Biotechnology, 12(19), 2685-2691, 2013. DOI: 10.5897/AJB2012.2989. |
[30] | O. L. Oke, “Eliminating cyanogens from cassava through processing: technology and tradition”. Acta Hortic., 375, 163–74, 1994. |
[31] | A. Westby, and B. K. Choo,“Cyanogen reduction during the lactic fermentation of cassava”. Acta Hort., 375, 209-215, 1994. |
[32] | S. Sundaresan, B. Nambisan, and C. S. Eswari Amma,“Bitterness in cassava in relation to cyanoglucoside content”. Indian J Agric Sci., 57, 37–40, 1987. |
[33] | P. A. Lancaster, and D. G. Coursey, “Traditional post-harvest technology of perishable tropical staples”. Fao Agricultural Services Bulletin No. 59. Food and Agriculture Organization of the United Nations, Rome, 1984. |
[34] | V. Ravindran, “Cassava leaves as animal feed: potential and limitations”. J. Sci. Food Agric. 61, 141-150, 1993. |
[35] | R. M. Gleadow, and I. E. Woodrow, “Mini-Review: constraints on effectiveness of cyanogenic glycosides in herbivore defense”. J. Chem. Ecol., 28, 1301-1313, 2002. |
[36] | S. K. Hahn, A. K. Howland, and J. E. Wilson, “Breeding of root and tuber crops”. Proc. 1st. Nat. Seminar on root tuber crops. NRCRI, Umudike, March 21-25, pp. 36-47, 1977. |
[37] | B. Oloya, C. Adaku, E. Ntambi, and M. Andama, “Detoxification of Nyar-Udota Cassava Variety in Zombo District by Fermentation”. International Journal of Nutrition and Food Sciences, 6(3), 118-121, 2017. |
[38] | R. D. Cooke, “Effect of cassava processing on residual cyanide”. In: F. Dalange, and A. Alhluwalia, (eds). Cassava toxicity and thyroid; research and public health issues; Proceedings of Workshop. Ottawa: IDRC-207c, 1983. |
APA Style
Morgan Andama, Benson Oloya. (2017). Effectiveness of Traditional Processing Techniques in Reducing Cyanide Levels in Selected Cassava Varieties in Zombo District, Uganda. International Journal of Food Science and Biotechnology, 2(4), 121-125. https://doi.org/10.11648/j.ijfsb.20170204.14
ACS Style
Morgan Andama; Benson Oloya. Effectiveness of Traditional Processing Techniques in Reducing Cyanide Levels in Selected Cassava Varieties in Zombo District, Uganda. Int. J. Food Sci. Biotechnol. 2017, 2(4), 121-125. doi: 10.11648/j.ijfsb.20170204.14
@article{10.11648/j.ijfsb.20170204.14, author = {Morgan Andama and Benson Oloya}, title = {Effectiveness of Traditional Processing Techniques in Reducing Cyanide Levels in Selected Cassava Varieties in Zombo District, Uganda}, journal = {International Journal of Food Science and Biotechnology}, volume = {2}, number = {4}, pages = {121-125}, doi = {10.11648/j.ijfsb.20170204.14}, url = {https://doi.org/10.11648/j.ijfsb.20170204.14}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20170204.14}, abstract = {The root tubers of cassava (Manihot esculenta Crantz) contain varying amounts of cyanogenic glucosides which liberate toxic hydrogen cyanide with the resultant effect of poisoning especially the highly cyanogenic cultivars. These include some of the local and improved cassava varieties grown in Zombo district, Uganda among others. Therefore, this study explored the effectiveness of some traditional processing techniques used singly and in combination to reduce cyanide levels in highly toxic root tubers of selected local (Nyar-anderiano, Nyar-papoga, Nyar-udota, Nyar-pamitu, Bisimwenge, Nyar-matia) and improved (NASE 3, NASE 9, NASE 14, NASE 19, TME 14 and TME 204) cassava varieties grown in Zombo District, Uganda. The processed and the fresh cassava tubers were analysed for Hydrogen cyanide (HCN) by the standard titration method. The results indicated that heap fermentation followed by sun-drying reduced the cyanide levels in all the selected improved varieties to WHO safe levels for consumption (below 10mg/kg) in the dried chips. Mixed processing methods combining soaking, fermentation and sun-drying reduced the cyanide concentrations in the local varieties (Nyar-udota, Nyar-anderiano) and improved variety (NASE 19) to World Health Organisation (WHO) safe levels. None of the processing techniques used singly reduced the HCN levels in the root tubers of the selected cassava varieties to safe levels. Hence mixed strategies combining several traditional cassava processing techniques should be adopted to detoxify the selected cassava varieties of cyanogens. The local cassava varieties (Bisimwenge, Nya-matia, Nya-pamitu, Nya-papoga) would require modifications of the combined traditional processing techniques or other more complex processes to detoxify them of HCN before human consumption.}, year = {2017} }
TY - JOUR T1 - Effectiveness of Traditional Processing Techniques in Reducing Cyanide Levels in Selected Cassava Varieties in Zombo District, Uganda AU - Morgan Andama AU - Benson Oloya Y1 - 2017/10/20 PY - 2017 N1 - https://doi.org/10.11648/j.ijfsb.20170204.14 DO - 10.11648/j.ijfsb.20170204.14 T2 - International Journal of Food Science and Biotechnology JF - International Journal of Food Science and Biotechnology JO - International Journal of Food Science and Biotechnology SP - 121 EP - 125 PB - Science Publishing Group SN - 2578-9643 UR - https://doi.org/10.11648/j.ijfsb.20170204.14 AB - The root tubers of cassava (Manihot esculenta Crantz) contain varying amounts of cyanogenic glucosides which liberate toxic hydrogen cyanide with the resultant effect of poisoning especially the highly cyanogenic cultivars. These include some of the local and improved cassava varieties grown in Zombo district, Uganda among others. Therefore, this study explored the effectiveness of some traditional processing techniques used singly and in combination to reduce cyanide levels in highly toxic root tubers of selected local (Nyar-anderiano, Nyar-papoga, Nyar-udota, Nyar-pamitu, Bisimwenge, Nyar-matia) and improved (NASE 3, NASE 9, NASE 14, NASE 19, TME 14 and TME 204) cassava varieties grown in Zombo District, Uganda. The processed and the fresh cassava tubers were analysed for Hydrogen cyanide (HCN) by the standard titration method. The results indicated that heap fermentation followed by sun-drying reduced the cyanide levels in all the selected improved varieties to WHO safe levels for consumption (below 10mg/kg) in the dried chips. Mixed processing methods combining soaking, fermentation and sun-drying reduced the cyanide concentrations in the local varieties (Nyar-udota, Nyar-anderiano) and improved variety (NASE 19) to World Health Organisation (WHO) safe levels. None of the processing techniques used singly reduced the HCN levels in the root tubers of the selected cassava varieties to safe levels. Hence mixed strategies combining several traditional cassava processing techniques should be adopted to detoxify the selected cassava varieties of cyanogens. The local cassava varieties (Bisimwenge, Nya-matia, Nya-pamitu, Nya-papoga) would require modifications of the combined traditional processing techniques or other more complex processes to detoxify them of HCN before human consumption. VL - 2 IS - 4 ER -