Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in <i>Bufonis Venenum</i>

Yi Luo; Cejia Liu; Shengci Fan; Zhaoqing Meng; Fei He

doi:doi:10.11648/j.ijcm.20200401.11

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Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum

Yi Luo, Cejia Liu, Shengci Fan, Zhaoqing Meng, Fei He

Published in International Journal of Chinese Medicine (Volume 4, Issue 1)

Received: 28 November 2019 Accepted: 18 December 2019 Published: 21 January 2020

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Abstract

The present study presents an effective and comprehensive evaluation method for assessing the quality of Bufonis Venenum, based on analysis of high performance liquid chromatography (HPLC) fingerprints in combination with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and a quantitative analysis multi-components by single marker (QAMS) method. Nine common peaks identified by fingerprint detection results of 10 batches of samples were collected and used for the similarity analysis, HCA, PCA and QAMS analysis. These methods drew a similar conclusion that the quantitative analysis multi-components by single marker (QAMS) method 41 Bufonis Venenum samples were categorized into four main groups by HCA and PCA, and the majority of the samples with similar ingredients were mainly concentrated in Shandong area. When QAMS method was compared with the external standard method (ESM), it was feasible to evaluate the quality of Bufonis Venenum by the values of relative correction factors (RCFs) from cinobufagin (the internal reference) versus arenobufagin, bufalin, bufotalin, resibufogenin and telocinobufogin. In conclusion, these methods were successfully applied to identification of the origin and evaluation the quality of Bufonis Venenum. Therefore, these evaluation methods are promising to be widely applied in the quality control of Traditional Chinese Medicines (TCMs).

Published in	International Journal of Chinese Medicine (Volume 4, Issue 1)
DOI	10.11648/j.ijcm.20200401.11
Page(s)	1-11
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

QAMS, Fingerprint, Bufadienolides, Bufonis Venenum, Method

References

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[5]	Song, H., Guo, T., Bi, K., Wang, H., et al. Determination of resibufogenin and cinobufagin in heart-protecting musk pill by HPLC [J]. Bio Chroma 2000, 14, 130–132.
[6]	Wu X. Y.; Gao H. M.; Wang Z. M. Advances on chemical constituents in crude medicines from toads [J]. Chin. J. Exp. Tradit. Med. Formulae 2010, 16, 207–214.
[7]	Liu J. S., Zhang D. M., Kurihara H., et al. Antitumor effects of venenum bufonis and its active components [J]. J. Int. Pharm. Res 2009, 2, 115-120.
[8]	Chen Y. L., Hao Y. Y., Guo F. J., et al. Research progress on chemical constituents and pharmacological activities of Bufonis Venenum [J]. Chin. Tra. Her. Drug 2017, 12, 2579-2587.
[9]	Wang Y. Study on chemical constituents of cardiac glycosides from Bufonis Venenum [D]. Jilin University 2015.
[10]	Guillermo S. H., Cristina Q., Gabriel V. A., et al. Antiproliferative activity and chemical composition of the venom from the Amazonian toad Rhinella marina (Anura: Bufonidae) [J]. Toxicon 2016, 121, 119-129.
[11]	Cui L. L., Zhang Y. Y., Shao W., et al. Analysis of the HPLC fingerprint and QAMS from Pyrrosia species [J]. Indus. Crop Pro 2016, 85, 29-37.
[12]	Zhu C. S., Li X. P., Zhang B., et al. Quantitative analysis of multi-components by single marker-a rational method for the internal quality of Chinese herbal medicine [J]. Integra. Med. Res 2017, 6, 1-11.
[13]	Kim S., Kim K. Y., Han C. S. et al. Simultaneous analysis of six major compounds in Osterici Radix and Notopterygii Rhizoma et Radix by HPLC and discrimination of their origins from chemical fingerprint analysis [J]. Arch. Pharm. Res. 2012, 35 (4), 691–699.
[14]	D. L., Liang Y. Z., Xie P. S. et al. Quality control and discrimination of pericarpium citri reticulatae and pericarpium citri reticulatae viride based on high-performance liquid chromatographic fingerprints and multivariate statistical analysis. [J] Anal. Chim. Acta, 2007, 588 (2), 207–215.
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[16]	Wang Z. M., Gao H. M., Fu X. T., et al. Study on the methodology of QAMS of the quality evaluation model of traditional Chinese medicine [J]. Chin. J. Chin. Mater. Med. 2006, 23, 1925-1928.

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APA Style

Yi Luo, Cejia Liu, Shengci Fan, Zhaoqing Meng, Fei He. (2020). Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum. International Journal of Chinese Medicine, 4(1), 1-11. https://doi.org/10.11648/j.ijcm.20200401.11

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ACS Style

Yi Luo; Cejia Liu; Shengci Fan; Zhaoqing Meng; Fei He. Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum. Int. J. Chin. Med. 2020, 4(1), 1-11. doi: 10.11648/j.ijcm.20200401.11

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AMA Style

Yi Luo, Cejia Liu, Shengci Fan, Zhaoqing Meng, Fei He. Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum. Int J Chin Med. 2020;4(1):1-11. doi: 10.11648/j.ijcm.20200401.11

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@article{10.11648/j.ijcm.20200401.11,
  author = {Yi Luo and Cejia Liu and Shengci Fan and Zhaoqing Meng and Fei He},
  title = {Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum},
  journal = {International Journal of Chinese Medicine},
  volume = {4},
  number = {1},
  pages = {1-11},
  doi = {10.11648/j.ijcm.20200401.11},
  url = {https://doi.org/10.11648/j.ijcm.20200401.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcm.20200401.11},
  abstract = {The present study presents an effective and comprehensive evaluation method for assessing the quality of Bufonis Venenum, based on analysis of high performance liquid chromatography (HPLC) fingerprints in combination with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and a quantitative analysis multi-components by single marker (QAMS) method. Nine common peaks identified by fingerprint detection results of 10 batches of samples were collected and used for the similarity analysis, HCA, PCA and QAMS analysis. These methods drew a similar conclusion that the quantitative analysis multi-components by single marker (QAMS) method 41 Bufonis Venenum samples were categorized into four main groups by HCA and PCA, and the majority of the samples with similar ingredients were mainly concentrated in Shandong area. When QAMS method was compared with the external standard method (ESM), it was feasible to evaluate the quality of Bufonis Venenum by the values of relative correction factors (RCFs) from cinobufagin (the internal reference) versus arenobufagin, bufalin, bufotalin, resibufogenin and telocinobufogin. In conclusion, these methods were successfully applied to identification of the origin and evaluation the quality of Bufonis Venenum. Therefore, these evaluation methods are promising to be widely applied in the quality control of Traditional Chinese Medicines (TCMs).},
 year = {2020}
}

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TY  - JOUR
T1  - Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum
AU  - Yi Luo
AU  - Cejia Liu
AU  - Shengci Fan
AU  - Zhaoqing Meng
AU  - Fei He
Y1  - 2020/01/21
PY  - 2020
N1  - https://doi.org/10.11648/j.ijcm.20200401.11
DO  - 10.11648/j.ijcm.20200401.11
T2  - International Journal of Chinese Medicine
JF  - International Journal of Chinese Medicine
JO  - International Journal of Chinese Medicine
SP  - 1
EP  - 11
PB  - Science Publishing Group
SN  - 2578-9473
UR  - https://doi.org/10.11648/j.ijcm.20200401.11
AB  - The present study presents an effective and comprehensive evaluation method for assessing the quality of Bufonis Venenum, based on analysis of high performance liquid chromatography (HPLC) fingerprints in combination with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and a quantitative analysis multi-components by single marker (QAMS) method. Nine common peaks identified by fingerprint detection results of 10 batches of samples were collected and used for the similarity analysis, HCA, PCA and QAMS analysis. These methods drew a similar conclusion that the quantitative analysis multi-components by single marker (QAMS) method 41 Bufonis Venenum samples were categorized into four main groups by HCA and PCA, and the majority of the samples with similar ingredients were mainly concentrated in Shandong area. When QAMS method was compared with the external standard method (ESM), it was feasible to evaluate the quality of Bufonis Venenum by the values of relative correction factors (RCFs) from cinobufagin (the internal reference) versus arenobufagin, bufalin, bufotalin, resibufogenin and telocinobufogin. In conclusion, these methods were successfully applied to identification of the origin and evaluation the quality of Bufonis Venenum. Therefore, these evaluation methods are promising to be widely applied in the quality control of Traditional Chinese Medicines (TCMs).
VL  - 4
IS  - 1
ER  -

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Author Information

Yi Luo

Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China
Cejia Liu

Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China
Shengci Fan

Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China
Zhaoqing Meng

Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China
Fei He

Traditional Chinese Medicine Institute, Shandong Hongjitang Pharmaceutical Group Co., LTD, Jinan, China

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APA Style

Yi Luo, Cejia Liu, Shengci Fan, Zhaoqing Meng, Fei He. (2020). Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum. International Journal of Chinese Medicine, 4(1), 1-11. https://doi.org/10.11648/j.ijcm.20200401.11

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ACS Style

Yi Luo; Cejia Liu; Shengci Fan; Zhaoqing Meng; Fei He. Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum. Int. J. Chin. Med. 2020, 4(1), 1-11. doi: 10.11648/j.ijcm.20200401.11

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AMA Style

Yi Luo, Cejia Liu, Shengci Fan, Zhaoqing Meng, Fei He. Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum. Int J Chin Med. 2020;4(1):1-11. doi: 10.11648/j.ijcm.20200401.11

Copy | Download

@article{10.11648/j.ijcm.20200401.11,
  author = {Yi Luo and Cejia Liu and Shengci Fan and Zhaoqing Meng and Fei He},
  title = {Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum},
  journal = {International Journal of Chinese Medicine},
  volume = {4},
  number = {1},
  pages = {1-11},
  doi = {10.11648/j.ijcm.20200401.11},
  url = {https://doi.org/10.11648/j.ijcm.20200401.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcm.20200401.11},
  abstract = {The present study presents an effective and comprehensive evaluation method for assessing the quality of Bufonis Venenum, based on analysis of high performance liquid chromatography (HPLC) fingerprints in combination with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and a quantitative analysis multi-components by single marker (QAMS) method. Nine common peaks identified by fingerprint detection results of 10 batches of samples were collected and used for the similarity analysis, HCA, PCA and QAMS analysis. These methods drew a similar conclusion that the quantitative analysis multi-components by single marker (QAMS) method 41 Bufonis Venenum samples were categorized into four main groups by HCA and PCA, and the majority of the samples with similar ingredients were mainly concentrated in Shandong area. When QAMS method was compared with the external standard method (ESM), it was feasible to evaluate the quality of Bufonis Venenum by the values of relative correction factors (RCFs) from cinobufagin (the internal reference) versus arenobufagin, bufalin, bufotalin, resibufogenin and telocinobufogin. In conclusion, these methods were successfully applied to identification of the origin and evaluation the quality of Bufonis Venenum. Therefore, these evaluation methods are promising to be widely applied in the quality control of Traditional Chinese Medicines (TCMs).},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Combination of HPLC Fingerprint and QAMS as a New Analytical Approach for Determination of Bufadienolides in Bufonis Venenum
AU  - Yi Luo
AU  - Cejia Liu
AU  - Shengci Fan
AU  - Zhaoqing Meng
AU  - Fei He
Y1  - 2020/01/21
PY  - 2020
N1  - https://doi.org/10.11648/j.ijcm.20200401.11
DO  - 10.11648/j.ijcm.20200401.11
T2  - International Journal of Chinese Medicine
JF  - International Journal of Chinese Medicine
JO  - International Journal of Chinese Medicine
SP  - 1
EP  - 11
PB  - Science Publishing Group
SN  - 2578-9473
UR  - https://doi.org/10.11648/j.ijcm.20200401.11
AB  - The present study presents an effective and comprehensive evaluation method for assessing the quality of Bufonis Venenum, based on analysis of high performance liquid chromatography (HPLC) fingerprints in combination with similarity analysis, hierarchical cluster analysis (HCA), principal component analysis (PCA) and a quantitative analysis multi-components by single marker (QAMS) method. Nine common peaks identified by fingerprint detection results of 10 batches of samples were collected and used for the similarity analysis, HCA, PCA and QAMS analysis. These methods drew a similar conclusion that the quantitative analysis multi-components by single marker (QAMS) method 41 Bufonis Venenum samples were categorized into four main groups by HCA and PCA, and the majority of the samples with similar ingredients were mainly concentrated in Shandong area. When QAMS method was compared with the external standard method (ESM), it was feasible to evaluate the quality of Bufonis Venenum by the values of relative correction factors (RCFs) from cinobufagin (the internal reference) versus arenobufagin, bufalin, bufotalin, resibufogenin and telocinobufogin. In conclusion, these methods were successfully applied to identification of the origin and evaluation the quality of Bufonis Venenum. Therefore, these evaluation methods are promising to be widely applied in the quality control of Traditional Chinese Medicines (TCMs).
VL  - 4
IS  - 1
ER  -

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