Sero-diagnosis of <i>Chlamydia trachomatis</i> and Molecular Detection of Genital Oncogenic <i>Human Papilloma Virus</i> Among Cameroonian Women

Bernard Wabo; Dickson Shey Nsagha; Théophile Njamen Nana; Clement Jules Nguedia Assob

doi:doi:10.11648/j.ejcbs.20200605.14

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Sero-diagnosis of Chlamydia trachomatis and Molecular Detection of Genital Oncogenic Human Papilloma Virus Among Cameroonian Women

Bernard Wabo, Dickson Shey Nsagha, Théophile Njamen Nana, Clement Jules Nguedia Assob

Published in European Journal of Clinical and Biomedical Sciences (Volume 6, Issue 5)

Received: 19 August 2020 Accepted: 2 September 2020 Published: 16 September 2020

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Abstract

Cervical cancer is a preventable public health concern ranking second among women’s cancer in Cameroon. Human papilloma virus (HPV) is the main causative agent with Chlamydia trachomatis being suggested as the co-factor. Our objective was to characterize high risk (hr) HPV types and to detect Chlamydia trachomatis antibodies among Cameroonian women with and without cervical cancer. Methods: This unmatched case-control study enrolled 100 cases with cervical cancer and 200 controls with normal cytology aged 25- 65 years in four reference hospitals in Douala and Yaoundé (Cameroon). Consented participants filled a structured questionnaire and data on socio-demographic characteristics collected. Chlamydia trachomatis antibodies were detected by the Enzyme Linked Immuno-Sorbent Assay technique (ELISA) and hr HPV- DNA by PCR technique. Descriptive statistics was conducted to provide frequencies and percentages and the logistic regression analysis to assess the association between categorical data. p < 0.05 was considered significant. Results: Our data showed 39 (39.0%) cases aged 39-52 years compared to 96 (48.0%) controls aged 25-38 years (p=0.001). We found 82 (82.0%) cases compared to 131 (65.5%) controls with hr HPV infections. HPV 16 was most prevalent being found in 29 (29.0%) cases compared to 69 (34.5%) controls. Chlamydia trachomatis IgG / hr HPV co-infections were detected in 20 (20.0%) cases compared to 33 (16.5%) controls but with no significant association with cervical cancer (aOR=1.87; 95%CI: 0.58-5.97; p=0.293). Chlamydia trachomatis IgM (aOR=3.50; 95%CI: 1.16-10.49; p=0.025) was significantly associated with cervical cancer. Conclusion: Hr HPV- DNA was high in cases than in controls. Chlamydia trachomatis single infection and Chlamydia trachomatis/hr HPV co-infections were not significantly associated to precancerous lesions thus, necessitating further investigations.

Published in	European Journal of Clinical and Biomedical Sciences (Volume 6, Issue 5)
DOI	10.11648/j.ejcbs.20200605.14
Page(s)	90-99
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Precancerous Lesions, Human papilloma virus, Chlamydia trachomatis, Co-infection, Case-control Study

References

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Bernard Wabo, Dickson Shey Nsagha, Théophile Njamen Nana, Clement Jules Nguedia Assob. (2020). Sero-diagnosis of Chlamydia trachomatis and Molecular Detection of Genital Oncogenic Human Papilloma Virus Among Cameroonian Women. European Journal of Clinical and Biomedical Sciences, 6(5), 90-99. https://doi.org/10.11648/j.ejcbs.20200605.14

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Bernard Wabo; Dickson Shey Nsagha; Théophile Njamen Nana; Clement Jules Nguedia Assob. Sero-diagnosis of Chlamydia trachomatis and Molecular Detection of Genital Oncogenic Human Papilloma Virus Among Cameroonian Women. Eur. J. Clin. Biomed. Sci. 2020, 6(5), 90-99. doi: 10.11648/j.ejcbs.20200605.14

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Bernard Wabo, Dickson Shey Nsagha, Théophile Njamen Nana, Clement Jules Nguedia Assob. Sero-diagnosis of Chlamydia trachomatis and Molecular Detection of Genital Oncogenic Human Papilloma Virus Among Cameroonian Women. Eur J Clin Biomed Sci. 2020;6(5):90-99. doi: 10.11648/j.ejcbs.20200605.14

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@article{10.11648/j.ejcbs.20200605.14,
  author = {Bernard Wabo and Dickson Shey Nsagha and Théophile Njamen Nana and Clement Jules Nguedia Assob},
  title = {Sero-diagnosis of Chlamydia trachomatis and Molecular Detection of Genital Oncogenic Human Papilloma Virus Among Cameroonian Women},
  journal = {European Journal of Clinical and Biomedical Sciences},
  volume = {6},
  number = {5},
  pages = {90-99},
  doi = {10.11648/j.ejcbs.20200605.14},
  url = {https://doi.org/10.11648/j.ejcbs.20200605.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20200605.14},
  abstract = {Cervical cancer is a preventable public health concern ranking second among women’s cancer in Cameroon. Human papilloma virus (HPV) is the main causative agent with Chlamydia trachomatis being suggested as the co-factor. Our objective was to characterize high risk (hr) HPV types and to detect Chlamydia trachomatis antibodies among Cameroonian women with and without cervical cancer. Methods: This unmatched case-control study enrolled 100 cases with cervical cancer and 200 controls with normal cytology aged 25- 65 years in four reference hospitals in Douala and Yaoundé (Cameroon). Consented participants filled a structured questionnaire and data on socio-demographic characteristics collected. Chlamydia trachomatis antibodies were detected by the Enzyme Linked Immuno-Sorbent Assay technique (ELISA) and hr HPV- DNA by PCR technique. Descriptive statistics was conducted to provide frequencies and percentages and the logistic regression analysis to assess the association between categorical data. p Results: Our data showed 39 (39.0%) cases aged 39-52 years compared to 96 (48.0%) controls aged 25-38 years (p=0.001). We found 82 (82.0%) cases compared to 131 (65.5%) controls with hr HPV infections. HPV 16 was most prevalent being found in 29 (29.0%) cases compared to 69 (34.5%) controls. Chlamydia trachomatis IgG / hr HPV co-infections were detected in 20 (20.0%) cases compared to 33 (16.5%) controls but with no significant association with cervical cancer (aOR=1.87; 95%CI: 0.58-5.97; p=0.293). Chlamydia trachomatis IgM (aOR=3.50; 95%CI: 1.16-10.49; p=0.025) was significantly associated with cervical cancer. Conclusion: Hr HPV- DNA was high in cases than in controls. Chlamydia trachomatis single infection and Chlamydia trachomatis/hr HPV co-infections were not significantly associated to precancerous lesions thus, necessitating further investigations.},
 year = {2020}
}

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TY  - JOUR
T1  - Sero-diagnosis of Chlamydia trachomatis and Molecular Detection of Genital Oncogenic Human Papilloma Virus Among Cameroonian Women
AU  - Bernard Wabo
AU  - Dickson Shey Nsagha
AU  - Théophile Njamen Nana
AU  - Clement Jules Nguedia Assob
Y1  - 2020/09/16
PY  - 2020
N1  - https://doi.org/10.11648/j.ejcbs.20200605.14
DO  - 10.11648/j.ejcbs.20200605.14
T2  - European Journal of Clinical and Biomedical Sciences
JF  - European Journal of Clinical and Biomedical Sciences
JO  - European Journal of Clinical and Biomedical Sciences
SP  - 90
EP  - 99
PB  - Science Publishing Group
SN  - 2575-5005
UR  - https://doi.org/10.11648/j.ejcbs.20200605.14
AB  - Cervical cancer is a preventable public health concern ranking second among women’s cancer in Cameroon. Human papilloma virus (HPV) is the main causative agent with Chlamydia trachomatis being suggested as the co-factor. Our objective was to characterize high risk (hr) HPV types and to detect Chlamydia trachomatis antibodies among Cameroonian women with and without cervical cancer. Methods: This unmatched case-control study enrolled 100 cases with cervical cancer and 200 controls with normal cytology aged 25- 65 years in four reference hospitals in Douala and Yaoundé (Cameroon). Consented participants filled a structured questionnaire and data on socio-demographic characteristics collected. Chlamydia trachomatis antibodies were detected by the Enzyme Linked Immuno-Sorbent Assay technique (ELISA) and hr HPV- DNA by PCR technique. Descriptive statistics was conducted to provide frequencies and percentages and the logistic regression analysis to assess the association between categorical data. p Results: Our data showed 39 (39.0%) cases aged 39-52 years compared to 96 (48.0%) controls aged 25-38 years (p=0.001). We found 82 (82.0%) cases compared to 131 (65.5%) controls with hr HPV infections. HPV 16 was most prevalent being found in 29 (29.0%) cases compared to 69 (34.5%) controls. Chlamydia trachomatis IgG / hr HPV co-infections were detected in 20 (20.0%) cases compared to 33 (16.5%) controls but with no significant association with cervical cancer (aOR=1.87; 95%CI: 0.58-5.97; p=0.293). Chlamydia trachomatis IgM (aOR=3.50; 95%CI: 1.16-10.49; p=0.025) was significantly associated with cervical cancer. Conclusion: Hr HPV- DNA was high in cases than in controls. Chlamydia trachomatis single infection and Chlamydia trachomatis/hr HPV co-infections were not significantly associated to precancerous lesions thus, necessitating further investigations.
VL  - 6
IS  - 5
ER  -

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Author Information

Bernard Wabo

Department of Medical Laboratory Science, Faculty of Health Sciences, University of Buea, Buea, Cameroon
Dickson Shey Nsagha

Department of Public Health and Hygiene, Faculty of Health Sciences, University of Buea, Buea, Cameroon
Théophile Njamen Nana

Department of Obstetrics and Gynaecology, Faculty of Health Sciences, University of Buea, Buea, Cameroon
Clement Jules Nguedia Assob

Department of Medical Laboratory Science, Faculty of Health Sciences, University of Buea, Buea, Cameroon

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Bernard Wabo, Dickson Shey Nsagha, Théophile Njamen Nana, Clement Jules Nguedia Assob. (2020). Sero-diagnosis of Chlamydia trachomatis and Molecular Detection of Genital Oncogenic Human Papilloma Virus Among Cameroonian Women. European Journal of Clinical and Biomedical Sciences, 6(5), 90-99. https://doi.org/10.11648/j.ejcbs.20200605.14

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Bernard Wabo; Dickson Shey Nsagha; Théophile Njamen Nana; Clement Jules Nguedia Assob. Sero-diagnosis of Chlamydia trachomatis and Molecular Detection of Genital Oncogenic Human Papilloma Virus Among Cameroonian Women. Eur. J. Clin. Biomed. Sci. 2020, 6(5), 90-99. doi: 10.11648/j.ejcbs.20200605.14

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AMA Style

Bernard Wabo, Dickson Shey Nsagha, Théophile Njamen Nana, Clement Jules Nguedia Assob. Sero-diagnosis of Chlamydia trachomatis and Molecular Detection of Genital Oncogenic Human Papilloma Virus Among Cameroonian Women. Eur J Clin Biomed Sci. 2020;6(5):90-99. doi: 10.11648/j.ejcbs.20200605.14

Copy | Download

@article{10.11648/j.ejcbs.20200605.14,
  author = {Bernard Wabo and Dickson Shey Nsagha and Théophile Njamen Nana and Clement Jules Nguedia Assob},
  title = {Sero-diagnosis of Chlamydia trachomatis and Molecular Detection of Genital Oncogenic Human Papilloma Virus Among Cameroonian Women},
  journal = {European Journal of Clinical and Biomedical Sciences},
  volume = {6},
  number = {5},
  pages = {90-99},
  doi = {10.11648/j.ejcbs.20200605.14},
  url = {https://doi.org/10.11648/j.ejcbs.20200605.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20200605.14},
  abstract = {Cervical cancer is a preventable public health concern ranking second among women’s cancer in Cameroon. Human papilloma virus (HPV) is the main causative agent with Chlamydia trachomatis being suggested as the co-factor. Our objective was to characterize high risk (hr) HPV types and to detect Chlamydia trachomatis antibodies among Cameroonian women with and without cervical cancer. Methods: This unmatched case-control study enrolled 100 cases with cervical cancer and 200 controls with normal cytology aged 25- 65 years in four reference hospitals in Douala and Yaoundé (Cameroon). Consented participants filled a structured questionnaire and data on socio-demographic characteristics collected. Chlamydia trachomatis antibodies were detected by the Enzyme Linked Immuno-Sorbent Assay technique (ELISA) and hr HPV- DNA by PCR technique. Descriptive statistics was conducted to provide frequencies and percentages and the logistic regression analysis to assess the association between categorical data. p Results: Our data showed 39 (39.0%) cases aged 39-52 years compared to 96 (48.0%) controls aged 25-38 years (p=0.001). We found 82 (82.0%) cases compared to 131 (65.5%) controls with hr HPV infections. HPV 16 was most prevalent being found in 29 (29.0%) cases compared to 69 (34.5%) controls. Chlamydia trachomatis IgG / hr HPV co-infections were detected in 20 (20.0%) cases compared to 33 (16.5%) controls but with no significant association with cervical cancer (aOR=1.87; 95%CI: 0.58-5.97; p=0.293). Chlamydia trachomatis IgM (aOR=3.50; 95%CI: 1.16-10.49; p=0.025) was significantly associated with cervical cancer. Conclusion: Hr HPV- DNA was high in cases than in controls. Chlamydia trachomatis single infection and Chlamydia trachomatis/hr HPV co-infections were not significantly associated to precancerous lesions thus, necessitating further investigations.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Sero-diagnosis of Chlamydia trachomatis and Molecular Detection of Genital Oncogenic Human Papilloma Virus Among Cameroonian Women
AU  - Bernard Wabo
AU  - Dickson Shey Nsagha
AU  - Théophile Njamen Nana
AU  - Clement Jules Nguedia Assob
Y1  - 2020/09/16
PY  - 2020
N1  - https://doi.org/10.11648/j.ejcbs.20200605.14
DO  - 10.11648/j.ejcbs.20200605.14
T2  - European Journal of Clinical and Biomedical Sciences
JF  - European Journal of Clinical and Biomedical Sciences
JO  - European Journal of Clinical and Biomedical Sciences
SP  - 90
EP  - 99
PB  - Science Publishing Group
SN  - 2575-5005
UR  - https://doi.org/10.11648/j.ejcbs.20200605.14
AB  - Cervical cancer is a preventable public health concern ranking second among women’s cancer in Cameroon. Human papilloma virus (HPV) is the main causative agent with Chlamydia trachomatis being suggested as the co-factor. Our objective was to characterize high risk (hr) HPV types and to detect Chlamydia trachomatis antibodies among Cameroonian women with and without cervical cancer. Methods: This unmatched case-control study enrolled 100 cases with cervical cancer and 200 controls with normal cytology aged 25- 65 years in four reference hospitals in Douala and Yaoundé (Cameroon). Consented participants filled a structured questionnaire and data on socio-demographic characteristics collected. Chlamydia trachomatis antibodies were detected by the Enzyme Linked Immuno-Sorbent Assay technique (ELISA) and hr HPV- DNA by PCR technique. Descriptive statistics was conducted to provide frequencies and percentages and the logistic regression analysis to assess the association between categorical data. p Results: Our data showed 39 (39.0%) cases aged 39-52 years compared to 96 (48.0%) controls aged 25-38 years (p=0.001). We found 82 (82.0%) cases compared to 131 (65.5%) controls with hr HPV infections. HPV 16 was most prevalent being found in 29 (29.0%) cases compared to 69 (34.5%) controls. Chlamydia trachomatis IgG / hr HPV co-infections were detected in 20 (20.0%) cases compared to 33 (16.5%) controls but with no significant association with cervical cancer (aOR=1.87; 95%CI: 0.58-5.97; p=0.293). Chlamydia trachomatis IgM (aOR=3.50; 95%CI: 1.16-10.49; p=0.025) was significantly associated with cervical cancer. Conclusion: Hr HPV- DNA was high in cases than in controls. Chlamydia trachomatis single infection and Chlamydia trachomatis/hr HPV co-infections were not significantly associated to precancerous lesions thus, necessitating further investigations.
VL  - 6
IS  - 5
ER  -

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