Risk Analysis and Antibiogram Spectrum of <i>Escherichia coli</i> O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria

Nfongeh Joseph Fuh; Owoseni Mojisola Christiana; Obande Godwin Attah; Upla Peter Uteh; Odonye Dauda Dantani; Fadayomi Victor Kolawole; Uchenwa Mercy Ogechi

doi:doi:10.11648/j.ejcbs.20180403.11

Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria

Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Obande Godwin Attah, Upla Peter Uteh, Odonye Dauda Dantani, Fadayomi Victor Kolawole, Uchenwa Mercy Ogechi

Published in European Journal of Clinical and Biomedical Sciences (Volume 4, Issue 3)

Received: 9 June 2018 Accepted: 24 July 2018 Published: 24 August 2018

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Abstract

This cross sectional study is aimed at evaluating the risk factors and antibiogram profiles of Escherichia coli O157:H7 in children stool and bovine meat obtained from selected households across Cross River State, Southern Nigeria. A total of 360 samples of fresh household bovine meat and 366 children diarrheal and nondiarrheal stool samples each were collected and examined for E. coli O157: H7 using standard culture and serological methods. Confirmed E. coli O157: H7 isolates were evaluated for antimicrobial susceptibility using the Agar disc diffusion method. The total positive samples for E. coli O157: H7 in household meat was 76/360 (21.11%,) while the diarrheaic and nondiarrheaic stool samples had 70/366 (19.13%) and 5/366 (1.37%) positive samples respectively. A significant difference was observed in the prevalence values among the bovine meat samples from various households and between the diarrheaic and nondiarrheaic samples at p<0.05. Risk factors such as Age range with highest prevalence value at 1-2yrs (26.83%); Occupation of parent/guardian with highest value from farming (25.67%) and Main domestic water source with highest value from surface water (28.21%) were observed to significantly affect the prevalence of the pathogen in children diarrheaic stool (p<0.05). All 70 diarrheaic isolates were resistant to one or multiple antibiotics with highest values obtained from tetracycline (88.6%) and cotrimoxazole (77.1%). This study revealed that bovine meat and some human and environmental factors play a vital role in the establishment of E. coli O157:H7 infection in children in the study communities with diarrheal stool being the main vehicle for secondary infections in humans. Cattle therefore serve as a major source of transmission of multi drug resistant E. coli O157:H7 to humans hence the need for continuous surveillance of this pathogen and implementation of legislation against indiscriminate use of antibiotics in diary farms.

Published in	European Journal of Clinical and Biomedical Sciences (Volume 4, Issue 3)
DOI	10.11648/j.ejcbs.20180403.11
Page(s)	39-45
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Escherichia coli O157:H7, Children Stool, Bovine Meat, Risk Factors, Antibiotics, Nigeria

References

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Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Obande Godwin Attah, Upla Peter Uteh, Odonye Dauda Dantani, et al. (2018). Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria. European Journal of Clinical and Biomedical Sciences, 4(3), 39-45. https://doi.org/10.11648/j.ejcbs.20180403.11

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Nfongeh Joseph Fuh; Owoseni Mojisola Christiana; Obande Godwin Attah; Upla Peter Uteh; Odonye Dauda Dantani, et al. Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria. Eur. J. Clin. Biomed. Sci. 2018, 4(3), 39-45. doi: 10.11648/j.ejcbs.20180403.11

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Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Obande Godwin Attah, Upla Peter Uteh, Odonye Dauda Dantani, et al. Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria. Eur J Clin Biomed Sci. 2018;4(3):39-45. doi: 10.11648/j.ejcbs.20180403.11

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@article{10.11648/j.ejcbs.20180403.11,
  author = {Nfongeh Joseph Fuh and Owoseni Mojisola Christiana and Obande Godwin Attah and Upla Peter Uteh and Odonye Dauda Dantani and Fadayomi Victor Kolawole and Uchenwa Mercy Ogechi},
  title = {Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria},
  journal = {European Journal of Clinical and Biomedical Sciences},
  volume = {4},
  number = {3},
  pages = {39-45},
  doi = {10.11648/j.ejcbs.20180403.11},
  url = {https://doi.org/10.11648/j.ejcbs.20180403.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20180403.11},
  abstract = {This cross sectional study is aimed at evaluating the risk factors and antibiogram profiles of Escherichia coli O157:H7 in children stool and bovine meat obtained from selected households across Cross River State, Southern Nigeria. A total of 360 samples of fresh household bovine meat and 366 children diarrheal and nondiarrheal stool samples each were collected and examined for E. coli O157: H7 using standard culture and serological methods. Confirmed E. coli O157: H7 isolates were evaluated for antimicrobial susceptibility using the Agar disc diffusion method. The total positive samples for E. coli O157: H7 in household meat was 76/360 (21.11%,) while the diarrheaic and nondiarrheaic stool samples had 70/366 (19.13%) and 5/366 (1.37%) positive samples respectively. A significant difference was observed in the prevalence values among the bovine meat samples from various households and between the diarrheaic and nondiarrheaic samples at pAge range with highest prevalence value at 1-2yrs (26.83%); Occupation of parent/guardian with highest value from farming (25.67%) and Main domestic water source with highest value from surface water (28.21%) were observed to significantly affect the prevalence of the pathogen in children diarrheaic stool (pE. coli O157:H7 infection in children in the study communities with diarrheal stool being the main vehicle for secondary infections in humans. Cattle therefore serve as a major source of transmission of multi drug resistant E. coli O157:H7 to humans hence the need for continuous surveillance of this pathogen and implementation of legislation against indiscriminate use of antibiotics in diary farms.},
 year = {2018}
}

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TY  - JOUR
T1  - Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria
AU  - Nfongeh Joseph Fuh
AU  - Owoseni Mojisola Christiana
AU  - Obande Godwin Attah
AU  - Upla Peter Uteh
AU  - Odonye Dauda Dantani
AU  - Fadayomi Victor Kolawole
AU  - Uchenwa Mercy Ogechi
Y1  - 2018/08/24
PY  - 2018
N1  - https://doi.org/10.11648/j.ejcbs.20180403.11
DO  - 10.11648/j.ejcbs.20180403.11
T2  - European Journal of Clinical and Biomedical Sciences
JF  - European Journal of Clinical and Biomedical Sciences
JO  - European Journal of Clinical and Biomedical Sciences
SP  - 39
EP  - 45
PB  - Science Publishing Group
SN  - 2575-5005
UR  - https://doi.org/10.11648/j.ejcbs.20180403.11
AB  - This cross sectional study is aimed at evaluating the risk factors and antibiogram profiles of Escherichia coli O157:H7 in children stool and bovine meat obtained from selected households across Cross River State, Southern Nigeria. A total of 360 samples of fresh household bovine meat and 366 children diarrheal and nondiarrheal stool samples each were collected and examined for E. coli O157: H7 using standard culture and serological methods. Confirmed E. coli O157: H7 isolates were evaluated for antimicrobial susceptibility using the Agar disc diffusion method. The total positive samples for E. coli O157: H7 in household meat was 76/360 (21.11%,) while the diarrheaic and nondiarrheaic stool samples had 70/366 (19.13%) and 5/366 (1.37%) positive samples respectively. A significant difference was observed in the prevalence values among the bovine meat samples from various households and between the diarrheaic and nondiarrheaic samples at pAge range with highest prevalence value at 1-2yrs (26.83%); Occupation of parent/guardian with highest value from farming (25.67%) and Main domestic water source with highest value from surface water (28.21%) were observed to significantly affect the prevalence of the pathogen in children diarrheaic stool (pE. coli O157:H7 infection in children in the study communities with diarrheal stool being the main vehicle for secondary infections in humans. Cattle therefore serve as a major source of transmission of multi drug resistant E. coli O157:H7 to humans hence the need for continuous surveillance of this pathogen and implementation of legislation against indiscriminate use of antibiotics in diary farms.
VL  - 4
IS  - 3
ER  -

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Author Information

Nfongeh Joseph Fuh

Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria
Owoseni Mojisola Christiana

Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria
Obande Godwin Attah

Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria
Upla Peter Uteh

Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria
Odonye Dauda Dantani

Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria
Fadayomi Victor Kolawole

Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria
Uchenwa Mercy Ogechi

Department of Microbiology, Faculty of Biological Sciences, University of Calabar, Calabar, Nigeria

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APA Style

Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Obande Godwin Attah, Upla Peter Uteh, Odonye Dauda Dantani, et al. (2018). Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria. European Journal of Clinical and Biomedical Sciences, 4(3), 39-45. https://doi.org/10.11648/j.ejcbs.20180403.11

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ACS Style

Nfongeh Joseph Fuh; Owoseni Mojisola Christiana; Obande Godwin Attah; Upla Peter Uteh; Odonye Dauda Dantani, et al. Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria. Eur. J. Clin. Biomed. Sci. 2018, 4(3), 39-45. doi: 10.11648/j.ejcbs.20180403.11

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AMA Style

Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Obande Godwin Attah, Upla Peter Uteh, Odonye Dauda Dantani, et al. Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria. Eur J Clin Biomed Sci. 2018;4(3):39-45. doi: 10.11648/j.ejcbs.20180403.11

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@article{10.11648/j.ejcbs.20180403.11,
  author = {Nfongeh Joseph Fuh and Owoseni Mojisola Christiana and Obande Godwin Attah and Upla Peter Uteh and Odonye Dauda Dantani and Fadayomi Victor Kolawole and Uchenwa Mercy Ogechi},
  title = {Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria},
  journal = {European Journal of Clinical and Biomedical Sciences},
  volume = {4},
  number = {3},
  pages = {39-45},
  doi = {10.11648/j.ejcbs.20180403.11},
  url = {https://doi.org/10.11648/j.ejcbs.20180403.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20180403.11},
  abstract = {This cross sectional study is aimed at evaluating the risk factors and antibiogram profiles of Escherichia coli O157:H7 in children stool and bovine meat obtained from selected households across Cross River State, Southern Nigeria. A total of 360 samples of fresh household bovine meat and 366 children diarrheal and nondiarrheal stool samples each were collected and examined for E. coli O157: H7 using standard culture and serological methods. Confirmed E. coli O157: H7 isolates were evaluated for antimicrobial susceptibility using the Agar disc diffusion method. The total positive samples for E. coli O157: H7 in household meat was 76/360 (21.11%,) while the diarrheaic and nondiarrheaic stool samples had 70/366 (19.13%) and 5/366 (1.37%) positive samples respectively. A significant difference was observed in the prevalence values among the bovine meat samples from various households and between the diarrheaic and nondiarrheaic samples at pAge range with highest prevalence value at 1-2yrs (26.83%); Occupation of parent/guardian with highest value from farming (25.67%) and Main domestic water source with highest value from surface water (28.21%) were observed to significantly affect the prevalence of the pathogen in children diarrheaic stool (pE. coli O157:H7 infection in children in the study communities with diarrheal stool being the main vehicle for secondary infections in humans. Cattle therefore serve as a major source of transmission of multi drug resistant E. coli O157:H7 to humans hence the need for continuous surveillance of this pathogen and implementation of legislation against indiscriminate use of antibiotics in diary farms.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Risk Analysis and Antibiogram Spectrum of Escherichia coli O157: H7 Serotype from Children Stool and Raw Bovine Meat in Households Across Cross River State, Nigeria
AU  - Nfongeh Joseph Fuh
AU  - Owoseni Mojisola Christiana
AU  - Obande Godwin Attah
AU  - Upla Peter Uteh
AU  - Odonye Dauda Dantani
AU  - Fadayomi Victor Kolawole
AU  - Uchenwa Mercy Ogechi
Y1  - 2018/08/24
PY  - 2018
N1  - https://doi.org/10.11648/j.ejcbs.20180403.11
DO  - 10.11648/j.ejcbs.20180403.11
T2  - European Journal of Clinical and Biomedical Sciences
JF  - European Journal of Clinical and Biomedical Sciences
JO  - European Journal of Clinical and Biomedical Sciences
SP  - 39
EP  - 45
PB  - Science Publishing Group
SN  - 2575-5005
UR  - https://doi.org/10.11648/j.ejcbs.20180403.11
AB  - This cross sectional study is aimed at evaluating the risk factors and antibiogram profiles of Escherichia coli O157:H7 in children stool and bovine meat obtained from selected households across Cross River State, Southern Nigeria. A total of 360 samples of fresh household bovine meat and 366 children diarrheal and nondiarrheal stool samples each were collected and examined for E. coli O157: H7 using standard culture and serological methods. Confirmed E. coli O157: H7 isolates were evaluated for antimicrobial susceptibility using the Agar disc diffusion method. The total positive samples for E. coli O157: H7 in household meat was 76/360 (21.11%,) while the diarrheaic and nondiarrheaic stool samples had 70/366 (19.13%) and 5/366 (1.37%) positive samples respectively. A significant difference was observed in the prevalence values among the bovine meat samples from various households and between the diarrheaic and nondiarrheaic samples at pAge range with highest prevalence value at 1-2yrs (26.83%); Occupation of parent/guardian with highest value from farming (25.67%) and Main domestic water source with highest value from surface water (28.21%) were observed to significantly affect the prevalence of the pathogen in children diarrheaic stool (pE. coli O157:H7 infection in children in the study communities with diarrheal stool being the main vehicle for secondary infections in humans. Cattle therefore serve as a major source of transmission of multi drug resistant E. coli O157:H7 to humans hence the need for continuous surveillance of this pathogen and implementation of legislation against indiscriminate use of antibiotics in diary farms.
VL  - 4
IS  - 3
ER  -

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