Phytochemical Screening and Anti-Tb Activity of Root Extracts of <i>Guiera senegalensis (J. F. Gmel) </i>

Adedayo Adebiyi; Rachael Ayo; Isaac Bello; James Habila

doi:doi:10.11648/j.bio.20150306.20

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Phytochemical Screening and Anti-Tb Activity of Root Extracts of Guiera senegalensis (J. F. Gmel)

Adedayo Adebiyi, Rachael Ayo, Isaac Bello, James Habila

Published in American Journal of Bioscience and Bioengineering (Volume 3, Issue 6)

Received: 10 November 2015 Accepted: 29 December 2015 Published: 27 January 2016

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Abstract

The root of Guiera senegalensis is thought to possess medicinal properties according to Nigerian folklore. This study was undertaken to appraise the phytochemical constituents from polar and non-polar extracts (n-hexane, ethyl acetate and methanol) and anti-tuberculosis activities. Data obtained revealed that saponin and tannin appear as the only phyto-compounds in hexane extract, alkaloids, flavonoids, steroids, tannins and terpenoids were detected in the ethyl acetate fraction while alkaloids, cardenolides, flavonoids, phlobatanins, saponins, steroids, tannins and terpenoids were detected in the methanol extracts. Microplate Alamar Blue Assay (MABA) used for sensitivity study of Mycobacterium tuberculosis with 10µg/ml rifampicin revealed that the methanol extract from the root of Guiera senegalensis gave 22.71 ± 0.47 mm zone of inhibition whereas the ethyl acetate extract gave a 7.23 ± 1.35 mm zone of inhibition in comparison to 33.70 ± 0.64 mm obtained from the control. The minimal inhibitory content (MIC) of the methanol and ethyl acetate extracts were recorded at 2.8 ± 1.52 and 40.01 ± 1.20, while that of rifampicin was 0.38 ± 1.40. The n-hexane extracts did not show any inhibition. The results obtained suggested that the root of the studied plant possess anti-tuberculosis activities with the major activity tailored to the phyto-constituents from the methanol extracts.

Published in	American Journal of Bioscience and Bioengineering (Volume 3, Issue 6)
DOI	10.11648/j.bio.20150306.20
Page(s)	208-213
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Phytochemical, Guiera senegalensis, Anti-Tb, Root Extract, Mycobacterium Tuberculosis

References

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APA Style

Adedayo Adebiyi, Rachael Ayo, Isaac Bello, James Habila. (2016). Phytochemical Screening and Anti-Tb Activity of Root Extracts of Guiera senegalensis (J. F. Gmel) . American Journal of Bioscience and Bioengineering, 3(6), 208-213. https://doi.org/10.11648/j.bio.20150306.20

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ACS Style

Adedayo Adebiyi; Rachael Ayo; Isaac Bello; James Habila. Phytochemical Screening and Anti-Tb Activity of Root Extracts of Guiera senegalensis (J. F. Gmel) . Am. J. BioSci. Bioeng. 2016, 3(6), 208-213. doi: 10.11648/j.bio.20150306.20

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AMA Style

Adedayo Adebiyi, Rachael Ayo, Isaac Bello, James Habila. Phytochemical Screening and Anti-Tb Activity of Root Extracts of Guiera senegalensis (J. F. Gmel) . Am J BioSci Bioeng. 2016;3(6):208-213. doi: 10.11648/j.bio.20150306.20

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@article{10.11648/j.bio.20150306.20,
  author = {Adedayo Adebiyi and Rachael Ayo and Isaac Bello and James Habila},
  title = {Phytochemical Screening and Anti-Tb Activity of Root Extracts of Guiera senegalensis (J. F. Gmel) },
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {3},
  number = {6},
  pages = {208-213},
  doi = {10.11648/j.bio.20150306.20},
  url = {https://doi.org/10.11648/j.bio.20150306.20},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150306.20},
  abstract = {The root of Guiera senegalensis is thought to possess medicinal properties according to Nigerian folklore. This study was undertaken to appraise the phytochemical constituents from polar and non-polar extracts (n-hexane, ethyl acetate and methanol) and anti-tuberculosis activities. Data obtained revealed that saponin and tannin appear as the only phyto-compounds in hexane extract, alkaloids, flavonoids, steroids, tannins and terpenoids were detected in the ethyl acetate fraction while alkaloids, cardenolides, flavonoids, phlobatanins, saponins, steroids, tannins and terpenoids were detected in the methanol extracts. Microplate Alamar Blue Assay (MABA) used for sensitivity study of Mycobacterium tuberculosis with 10µg/ml rifampicin revealed that the methanol extract from the root of Guiera senegalensis gave 22.71 ± 0.47 mm zone of inhibition whereas the ethyl acetate extract gave a 7.23 ± 1.35 mm zone of inhibition in comparison to 33.70 ± 0.64 mm obtained from the control. The minimal inhibitory content (MIC) of the methanol and ethyl acetate extracts were recorded at 2.8 ± 1.52 and 40.01 ± 1.20, while that of rifampicin was 0.38 ± 1.40. The n-hexane extracts did not show any inhibition. The results obtained suggested that the root of the studied plant possess anti-tuberculosis activities with the major activity tailored to the phyto-constituents from the methanol extracts.},
 year = {2016}
}

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TY  - JOUR
T1  - Phytochemical Screening and Anti-Tb Activity of Root Extracts of Guiera senegalensis (J. F. Gmel) 
AU  - Adedayo Adebiyi
AU  - Rachael Ayo
AU  - Isaac Bello
AU  - James Habila
Y1  - 2016/01/27
PY  - 2016
N1  - https://doi.org/10.11648/j.bio.20150306.20
DO  - 10.11648/j.bio.20150306.20
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 208
EP  - 213
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20150306.20
AB  - The root of Guiera senegalensis is thought to possess medicinal properties according to Nigerian folklore. This study was undertaken to appraise the phytochemical constituents from polar and non-polar extracts (n-hexane, ethyl acetate and methanol) and anti-tuberculosis activities. Data obtained revealed that saponin and tannin appear as the only phyto-compounds in hexane extract, alkaloids, flavonoids, steroids, tannins and terpenoids were detected in the ethyl acetate fraction while alkaloids, cardenolides, flavonoids, phlobatanins, saponins, steroids, tannins and terpenoids were detected in the methanol extracts. Microplate Alamar Blue Assay (MABA) used for sensitivity study of Mycobacterium tuberculosis with 10µg/ml rifampicin revealed that the methanol extract from the root of Guiera senegalensis gave 22.71 ± 0.47 mm zone of inhibition whereas the ethyl acetate extract gave a 7.23 ± 1.35 mm zone of inhibition in comparison to 33.70 ± 0.64 mm obtained from the control. The minimal inhibitory content (MIC) of the methanol and ethyl acetate extracts were recorded at 2.8 ± 1.52 and 40.01 ± 1.20, while that of rifampicin was 0.38 ± 1.40. The n-hexane extracts did not show any inhibition. The results obtained suggested that the root of the studied plant possess anti-tuberculosis activities with the major activity tailored to the phyto-constituents from the methanol extracts.
VL  - 3
IS  - 6
ER  -

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Author Information

Adedayo Adebiyi

Chemistry Advanced Laboratory, Sheda Science & Technology Complex (SHESTCO), P. M. B., Garki – Abuja, Nigeria
Rachael Ayo

Department of Chemistry, Ahmadu Bello University (ABU), Zaria, Nigeria
Isaac Bello

Department of Chemistry, Ahmadu Bello University (ABU), Zaria, Nigeria
James Habila

Department of Chemistry, Ahmadu Bello University (ABU), Zaria, Nigeria

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APA Style

Adedayo Adebiyi, Rachael Ayo, Isaac Bello, James Habila. (2016). Phytochemical Screening and Anti-Tb Activity of Root Extracts of Guiera senegalensis (J. F. Gmel) . American Journal of Bioscience and Bioengineering, 3(6), 208-213. https://doi.org/10.11648/j.bio.20150306.20

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ACS Style

Adedayo Adebiyi; Rachael Ayo; Isaac Bello; James Habila. Phytochemical Screening and Anti-Tb Activity of Root Extracts of Guiera senegalensis (J. F. Gmel) . Am. J. BioSci. Bioeng. 2016, 3(6), 208-213. doi: 10.11648/j.bio.20150306.20

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AMA Style

Adedayo Adebiyi, Rachael Ayo, Isaac Bello, James Habila. Phytochemical Screening and Anti-Tb Activity of Root Extracts of Guiera senegalensis (J. F. Gmel) . Am J BioSci Bioeng. 2016;3(6):208-213. doi: 10.11648/j.bio.20150306.20

Copy | Download

@article{10.11648/j.bio.20150306.20,
  author = {Adedayo Adebiyi and Rachael Ayo and Isaac Bello and James Habila},
  title = {Phytochemical Screening and Anti-Tb Activity of Root Extracts of Guiera senegalensis (J. F. Gmel) },
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {3},
  number = {6},
  pages = {208-213},
  doi = {10.11648/j.bio.20150306.20},
  url = {https://doi.org/10.11648/j.bio.20150306.20},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150306.20},
  abstract = {The root of Guiera senegalensis is thought to possess medicinal properties according to Nigerian folklore. This study was undertaken to appraise the phytochemical constituents from polar and non-polar extracts (n-hexane, ethyl acetate and methanol) and anti-tuberculosis activities. Data obtained revealed that saponin and tannin appear as the only phyto-compounds in hexane extract, alkaloids, flavonoids, steroids, tannins and terpenoids were detected in the ethyl acetate fraction while alkaloids, cardenolides, flavonoids, phlobatanins, saponins, steroids, tannins and terpenoids were detected in the methanol extracts. Microplate Alamar Blue Assay (MABA) used for sensitivity study of Mycobacterium tuberculosis with 10µg/ml rifampicin revealed that the methanol extract from the root of Guiera senegalensis gave 22.71 ± 0.47 mm zone of inhibition whereas the ethyl acetate extract gave a 7.23 ± 1.35 mm zone of inhibition in comparison to 33.70 ± 0.64 mm obtained from the control. The minimal inhibitory content (MIC) of the methanol and ethyl acetate extracts were recorded at 2.8 ± 1.52 and 40.01 ± 1.20, while that of rifampicin was 0.38 ± 1.40. The n-hexane extracts did not show any inhibition. The results obtained suggested that the root of the studied plant possess anti-tuberculosis activities with the major activity tailored to the phyto-constituents from the methanol extracts.},
 year = {2016}
}

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TY  - JOUR
T1  - Phytochemical Screening and Anti-Tb Activity of Root Extracts of Guiera senegalensis (J. F. Gmel) 
AU  - Adedayo Adebiyi
AU  - Rachael Ayo
AU  - Isaac Bello
AU  - James Habila
Y1  - 2016/01/27
PY  - 2016
N1  - https://doi.org/10.11648/j.bio.20150306.20
DO  - 10.11648/j.bio.20150306.20
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 208
EP  - 213
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20150306.20
AB  - The root of Guiera senegalensis is thought to possess medicinal properties according to Nigerian folklore. This study was undertaken to appraise the phytochemical constituents from polar and non-polar extracts (n-hexane, ethyl acetate and methanol) and anti-tuberculosis activities. Data obtained revealed that saponin and tannin appear as the only phyto-compounds in hexane extract, alkaloids, flavonoids, steroids, tannins and terpenoids were detected in the ethyl acetate fraction while alkaloids, cardenolides, flavonoids, phlobatanins, saponins, steroids, tannins and terpenoids were detected in the methanol extracts. Microplate Alamar Blue Assay (MABA) used for sensitivity study of Mycobacterium tuberculosis with 10µg/ml rifampicin revealed that the methanol extract from the root of Guiera senegalensis gave 22.71 ± 0.47 mm zone of inhibition whereas the ethyl acetate extract gave a 7.23 ± 1.35 mm zone of inhibition in comparison to 33.70 ± 0.64 mm obtained from the control. The minimal inhibitory content (MIC) of the methanol and ethyl acetate extracts were recorded at 2.8 ± 1.52 and 40.01 ± 1.20, while that of rifampicin was 0.38 ± 1.40. The n-hexane extracts did not show any inhibition. The results obtained suggested that the root of the studied plant possess anti-tuberculosis activities with the major activity tailored to the phyto-constituents from the methanol extracts.
VL  - 3
IS  - 6
ER  -

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