Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish <i>Heteropneustes Fossilis</i>

Rakesh Kumar Pandey; Vijai Krishna Das

doi:doi:10.11648/j.bio.20150305.22

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Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis

Rakesh Kumar Pandey, Vijai Krishna Das

Published in American Journal of Bioscience and Bioengineering (Volume 3, Issue 5)

Received: 14 September 2015 Accepted: 30 September 2015 Published: 28 October 2015

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Abstract

Ultimobranchial gland in fishes is associated primarily with calcium regulation. The study was aimed to find effect of an organophosphate pesticide dimethoate on serum Ca²⁺ and ultimobranchial gland in freshwater catfish Heteropneustes fossilis. Fish were exposed to dimethoate at 2.24 mg/l (75% of 96 h LC₅₀) for short-term (24, 48, 72 and 96 h), and 25% of 96 h LC₅₀ (1.00 mg/l) for long-term (6, 12, 24 and 36 d). The blood samples collected at assigned durations from the anaesthetized fish were used to measure serum Ca²⁺ levels. At the same time UBG were also extricated from the fish by removing the tissues adjoining esophagus, liver and sinus venosus of the heart for subsequent histological examination. A significant (P<0.05) increase in serum Ca²⁺ levels of fish following 24 h and 6d dimethoate exposure was observed. However, after 48 h and 12 d the Ca²⁺ level gradually begins to decline exhibiting significant (P<0.0001) hypocalcaemia after 96 h and 36 d in the exposed fishes. The histological examination of exposed fish glands exhibit significant changes in their cellular activity, staining property, nuclear diameter and nuclear volume, vacuolization and degeneration. It is concluded that dimethoate severely hampers the calcium homeostasis in Heteropneustes fossilis; therefore the pesticide should be cautiously used near aquatic bodies.

Published in	American Journal of Bioscience and Bioengineering (Volume 3, Issue 5)
DOI	10.11648/j.bio.20150305.22
Page(s)	91-98
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Ultimobranchial Gland, Dimethoate, Calcium, Heteropneustes fossilis

References

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APA Style

Rakesh Kumar Pandey, Vijai Krishna Das. (2015). Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis. American Journal of Bioscience and Bioengineering, 3(5), 91-98. https://doi.org/10.11648/j.bio.20150305.22

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ACS Style

Rakesh Kumar Pandey; Vijai Krishna Das. Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis. Am. J. BioSci. Bioeng. 2015, 3(5), 91-98. doi: 10.11648/j.bio.20150305.22

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AMA Style

Rakesh Kumar Pandey, Vijai Krishna Das. Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis. Am J BioSci Bioeng. 2015;3(5):91-98. doi: 10.11648/j.bio.20150305.22

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@article{10.11648/j.bio.20150305.22,
  author = {Rakesh Kumar Pandey and Vijai Krishna Das},
  title = {Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {3},
  number = {5},
  pages = {91-98},
  doi = {10.11648/j.bio.20150305.22},
  url = {https://doi.org/10.11648/j.bio.20150305.22},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150305.22},
  abstract = {Ultimobranchial gland in fishes is associated primarily with calcium regulation. The study was aimed to find effect of an organophosphate pesticide dimethoate on serum Ca2+ and ultimobranchial gland in freshwater catfish Heteropneustes fossilis. Fish were exposed to dimethoate at 2.24 mg/l (75% of 96 h LC50) for short-term (24, 48, 72 and 96 h), and 25% of 96 h LC50 (1.00 mg/l) for long-term (6, 12, 24 and 36 d). The blood samples collected at assigned durations from the anaesthetized fish were used to measure serum Ca2+ levels. At the same time UBG were also extricated from the fish by removing the tissues adjoining esophagus, liver and sinus venosus of the heart for subsequent histological examination. A significant (P<0.05) increase in serum Ca2+ levels of fish following 24 h and 6d dimethoate exposure was observed. However, after 48 h and 12 d the Ca2+ level gradually begins to decline exhibiting significant (P<0.0001) hypocalcaemia after 96 h and 36 d in the exposed fishes. The histological examination of exposed fish glands exhibit significant changes in their cellular activity, staining property, nuclear diameter and nuclear volume, vacuolization and degeneration. It is concluded that dimethoate severely hampers the calcium homeostasis in Heteropneustes fossilis; therefore the pesticide should be cautiously used near aquatic bodies.},
 year = {2015}
}

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TY  - JOUR
T1  - Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis
AU  - Rakesh Kumar Pandey
AU  - Vijai Krishna Das
Y1  - 2015/10/28
PY  - 2015
N1  - https://doi.org/10.11648/j.bio.20150305.22
DO  - 10.11648/j.bio.20150305.22
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 91
EP  - 98
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20150305.22
AB  - Ultimobranchial gland in fishes is associated primarily with calcium regulation. The study was aimed to find effect of an organophosphate pesticide dimethoate on serum Ca2+ and ultimobranchial gland in freshwater catfish Heteropneustes fossilis. Fish were exposed to dimethoate at 2.24 mg/l (75% of 96 h LC50) for short-term (24, 48, 72 and 96 h), and 25% of 96 h LC50 (1.00 mg/l) for long-term (6, 12, 24 and 36 d). The blood samples collected at assigned durations from the anaesthetized fish were used to measure serum Ca2+ levels. At the same time UBG were also extricated from the fish by removing the tissues adjoining esophagus, liver and sinus venosus of the heart for subsequent histological examination. A significant (P<0.05) increase in serum Ca2+ levels of fish following 24 h and 6d dimethoate exposure was observed. However, after 48 h and 12 d the Ca2+ level gradually begins to decline exhibiting significant (P<0.0001) hypocalcaemia after 96 h and 36 d in the exposed fishes. The histological examination of exposed fish glands exhibit significant changes in their cellular activity, staining property, nuclear diameter and nuclear volume, vacuolization and degeneration. It is concluded that dimethoate severely hampers the calcium homeostasis in Heteropneustes fossilis; therefore the pesticide should be cautiously used near aquatic bodies.
VL  - 3
IS  - 5
ER  -

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Author Information

Rakesh Kumar Pandey

Department of Zoology, Kamla Nehru Institute of Physical and Social Sciences, Sultanpur U.P., India
Vijai Krishna Das

Department of Zoology, Kamla Nehru Institute of Physical and Social Sciences, Sultanpur U.P., India

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Rakesh Kumar Pandey, Vijai Krishna Das. (2015). Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis. American Journal of Bioscience and Bioengineering, 3(5), 91-98. https://doi.org/10.11648/j.bio.20150305.22

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ACS Style

Rakesh Kumar Pandey; Vijai Krishna Das. Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis. Am. J. BioSci. Bioeng. 2015, 3(5), 91-98. doi: 10.11648/j.bio.20150305.22

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AMA Style

Rakesh Kumar Pandey, Vijai Krishna Das. Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis. Am J BioSci Bioeng. 2015;3(5):91-98. doi: 10.11648/j.bio.20150305.22

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@article{10.11648/j.bio.20150305.22,
  author = {Rakesh Kumar Pandey and Vijai Krishna Das},
  title = {Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {3},
  number = {5},
  pages = {91-98},
  doi = {10.11648/j.bio.20150305.22},
  url = {https://doi.org/10.11648/j.bio.20150305.22},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150305.22},
  abstract = {Ultimobranchial gland in fishes is associated primarily with calcium regulation. The study was aimed to find effect of an organophosphate pesticide dimethoate on serum Ca2+ and ultimobranchial gland in freshwater catfish Heteropneustes fossilis. Fish were exposed to dimethoate at 2.24 mg/l (75% of 96 h LC50) for short-term (24, 48, 72 and 96 h), and 25% of 96 h LC50 (1.00 mg/l) for long-term (6, 12, 24 and 36 d). The blood samples collected at assigned durations from the anaesthetized fish were used to measure serum Ca2+ levels. At the same time UBG were also extricated from the fish by removing the tissues adjoining esophagus, liver and sinus venosus of the heart for subsequent histological examination. A significant (P<0.05) increase in serum Ca2+ levels of fish following 24 h and 6d dimethoate exposure was observed. However, after 48 h and 12 d the Ca2+ level gradually begins to decline exhibiting significant (P<0.0001) hypocalcaemia after 96 h and 36 d in the exposed fishes. The histological examination of exposed fish glands exhibit significant changes in their cellular activity, staining property, nuclear diameter and nuclear volume, vacuolization and degeneration. It is concluded that dimethoate severely hampers the calcium homeostasis in Heteropneustes fossilis; therefore the pesticide should be cautiously used near aquatic bodies.},
 year = {2015}
}

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TY  - JOUR
T1  - Histological Changes in Calcium Regulating Endocrine Gland (Ultimobranchial) After Dimethoate Exposure in Freshwater Catfish Heteropneustes Fossilis
AU  - Rakesh Kumar Pandey
AU  - Vijai Krishna Das
Y1  - 2015/10/28
PY  - 2015
N1  - https://doi.org/10.11648/j.bio.20150305.22
DO  - 10.11648/j.bio.20150305.22
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 91
EP  - 98
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20150305.22
AB  - Ultimobranchial gland in fishes is associated primarily with calcium regulation. The study was aimed to find effect of an organophosphate pesticide dimethoate on serum Ca2+ and ultimobranchial gland in freshwater catfish Heteropneustes fossilis. Fish were exposed to dimethoate at 2.24 mg/l (75% of 96 h LC50) for short-term (24, 48, 72 and 96 h), and 25% of 96 h LC50 (1.00 mg/l) for long-term (6, 12, 24 and 36 d). The blood samples collected at assigned durations from the anaesthetized fish were used to measure serum Ca2+ levels. At the same time UBG were also extricated from the fish by removing the tissues adjoining esophagus, liver and sinus venosus of the heart for subsequent histological examination. A significant (P<0.05) increase in serum Ca2+ levels of fish following 24 h and 6d dimethoate exposure was observed. However, after 48 h and 12 d the Ca2+ level gradually begins to decline exhibiting significant (P<0.0001) hypocalcaemia after 96 h and 36 d in the exposed fishes. The histological examination of exposed fish glands exhibit significant changes in their cellular activity, staining property, nuclear diameter and nuclear volume, vacuolization and degeneration. It is concluded that dimethoate severely hampers the calcium homeostasis in Heteropneustes fossilis; therefore the pesticide should be cautiously used near aquatic bodies.
VL  - 3
IS  - 5
ER  -

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