In several parasitological diseases, as for leishmaniasis, measurement of the size of cutaneous lesions, which develop at the site of parasite inoculation in animal models, are the most commonly used index to assess disease progression, to compare parasites pathogenicity or virulence and to determine the effects of drug treatment and immunotherapies. The aim of this study is to compare the accuracy of two measurement tools i.e., the caliper and the plethysmometer to refine the lesion size determination. Our findings showed that the use of plethysmometer produced higher correlation with the importance of the lesion mass at experimental endpoints. These findings suggest that, for better differentiation in drug monitoring or Leishmania (L. ) strains’ virulence and pathogenicity, plethysmometer method is more sensitive to detect parasite-induced swelling and lesions differences at the end of experimental protocols when lesion size is important but caliper is more indicated for small lesions.
| Published in | Animal and Veterinary Sciences (Volume 3, Issue 6) |
| DOI | 10.11648/j.avs.20150306.16 |
| Page(s) | 179-185 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Leishmania major, BALB/c Experimental Model, Lesion Size, Caliper, Plethysmometer
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APA Style
Hanene Attia, Aymen Bali, Rabiaa M. Sghaier, Pablo A. Leon Martinez, Ghada Mkannez, et al. (2016). Comparison Between Plethysmometer and Caliper Methods to Monitor Lesion-Size Induced by Leishmania major Infection in BALB/c Mouse Experimental Model. Animal and Veterinary Sciences, 3(6), 179-185. https://doi.org/10.11648/j.avs.20150306.16
ACS Style
Hanene Attia; Aymen Bali; Rabiaa M. Sghaier; Pablo A. Leon Martinez; Ghada Mkannez, et al. Comparison Between Plethysmometer and Caliper Methods to Monitor Lesion-Size Induced by Leishmania major Infection in BALB/c Mouse Experimental Model. Anim. Vet. Sci. 2016, 3(6), 179-185. doi: 10.11648/j.avs.20150306.16
AMA Style
Hanene Attia, Aymen Bali, Rabiaa M. Sghaier, Pablo A. Leon Martinez, Ghada Mkannez, et al. Comparison Between Plethysmometer and Caliper Methods to Monitor Lesion-Size Induced by Leishmania major Infection in BALB/c Mouse Experimental Model. Anim Vet Sci. 2016;3(6):179-185. doi: 10.11648/j.avs.20150306.16
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Download@article{10.11648/j.avs.20150306.16,
author = {Hanene Attia and Aymen Bali and Rabiaa M. Sghaier and Pablo A. Leon Martinez and Ghada Mkannez and Chiraz Atri and Khaled Chourabi and Fatma Z. Guerfali and Dhafer Laouini},
title = {Comparison Between Plethysmometer and Caliper Methods to Monitor Lesion-Size Induced by Leishmania major Infection in BALB/c Mouse Experimental Model},
journal = {Animal and Veterinary Sciences},
volume = {3},
number = {6},
pages = {179-185},
doi = {10.11648/j.avs.20150306.16},
url = {https://doi.org/10.11648/j.avs.20150306.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150306.16},
abstract = {In several parasitological diseases, as for leishmaniasis, measurement of the size of cutaneous lesions, which develop at the site of parasite inoculation in animal models, are the most commonly used index to assess disease progression, to compare parasites pathogenicity or virulence and to determine the effects of drug treatment and immunotherapies. The aim of this study is to compare the accuracy of two measurement tools i.e., the caliper and the plethysmometer to refine the lesion size determination. Our findings showed that the use of plethysmometer produced higher correlation with the importance of the lesion mass at experimental endpoints. These findings suggest that, for better differentiation in drug monitoring or Leishmania (L. ) strains’ virulence and pathogenicity, plethysmometer method is more sensitive to detect parasite-induced swelling and lesions differences at the end of experimental protocols when lesion size is important but caliper is more indicated for small lesions.},
year = {2016}
}
TY - JOUR T1 - Comparison Between Plethysmometer and Caliper Methods to Monitor Lesion-Size Induced by Leishmania major Infection in BALB/c Mouse Experimental Model AU - Hanene Attia AU - Aymen Bali AU - Rabiaa M. Sghaier AU - Pablo A. Leon Martinez AU - Ghada Mkannez AU - Chiraz Atri AU - Khaled Chourabi AU - Fatma Z. Guerfali AU - Dhafer Laouini Y1 - 2016/01/08 PY - 2016 N1 - https://doi.org/10.11648/j.avs.20150306.16 DO - 10.11648/j.avs.20150306.16 T2 - Animal and Veterinary Sciences JF - Animal and Veterinary Sciences JO - Animal and Veterinary Sciences SP - 179 EP - 185 PB - Science Publishing Group SN - 2328-5850 UR - https://doi.org/10.11648/j.avs.20150306.16 AB - In several parasitological diseases, as for leishmaniasis, measurement of the size of cutaneous lesions, which develop at the site of parasite inoculation in animal models, are the most commonly used index to assess disease progression, to compare parasites pathogenicity or virulence and to determine the effects of drug treatment and immunotherapies. The aim of this study is to compare the accuracy of two measurement tools i.e., the caliper and the plethysmometer to refine the lesion size determination. Our findings showed that the use of plethysmometer produced higher correlation with the importance of the lesion mass at experimental endpoints. These findings suggest that, for better differentiation in drug monitoring or Leishmania (L. ) strains’ virulence and pathogenicity, plethysmometer method is more sensitive to detect parasite-induced swelling and lesions differences at the end of experimental protocols when lesion size is important but caliper is more indicated for small lesions. VL - 3 IS - 6 ER -
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