Uncontrolled use of antibiotics in the treatment of calves suffering from gastroenteritis and dairy cows suffering from mastitis, created in pathogenic staphylococci enterotoxin producers some resistance to these drugs. These pathogens enterotoxin producers are often excreted with milk from cows with mastitis. That is why the specific and topical issue remains the isolation and identification of strains producing staphylococcal enterotoxin from cow milk and faeces of calves suffering from enteritis. Given all this, we have set a goal to get staphylococcal enterotoxin type D for use as mastitis diagnostic kit and enteritis and antigen for obtaining the antiserum. To obtain the staphylococcal enterotoxin type D we used strain S. aureus No. 494 (ATSS23235) courtesy offered by Dr. FC Fluer from Institute of Experimental Medicine, NF Gamaley, Medical Academy (USSR). Verification of the specificity of the enterotoxin) was performed using 5 strains of S.aureus producing different enterotoxins (entérotoxin- A; enterotoxin-B; enterotoxin C-1; C-2 and enterotoxin - E) as well as non-enterotoxigenic staphylococcal strain (S. aureus Wood 46). In addition to the staphylococcal strains, five strains of Escherichia coli, producing thermostable and thermolabile enterotoxins were also used in the experiment. The method of purification of enterotoxin-D by precipitation twice chromatography on km - cellulose and gel filtration on Sephadex G-75 allows to obtain pure enterotoxin-D having the biological properties, immunological and antigenic characteristics. Immunization of rabbits with purified enterotoxin-D associated with incomplete Freund's adjuvant provides antiserum very active and specific.
| Published in | Animal and Veterinary Sciences (Volume 3, Issue 6) |
| DOI | 10.11648/j.avs.20150306.14 |
| Page(s) | 166-170 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Staphylococcus aureus, Milk, faeces, Enterotoxin, Antiserum, Enteritis
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APA Style
Bidjeh Kebkiba, Ban-Bo Bebanto Antipas, Podkapaev Vladimir Michaelovich. (2015). Purification of Staphylococcal Enterotoxin Type D and Obtaining Its Antiserum. Animal and Veterinary Sciences, 3(6), 166-170. https://doi.org/10.11648/j.avs.20150306.14
ACS Style
Bidjeh Kebkiba; Ban-Bo Bebanto Antipas; Podkapaev Vladimir Michaelovich. Purification of Staphylococcal Enterotoxin Type D and Obtaining Its Antiserum. Anim. Vet. Sci. 2015, 3(6), 166-170. doi: 10.11648/j.avs.20150306.14
AMA Style
Bidjeh Kebkiba, Ban-Bo Bebanto Antipas, Podkapaev Vladimir Michaelovich. Purification of Staphylococcal Enterotoxin Type D and Obtaining Its Antiserum. Anim Vet Sci. 2015;3(6):166-170. doi: 10.11648/j.avs.20150306.14
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Download@article{10.11648/j.avs.20150306.14,
author = {Bidjeh Kebkiba and Ban-Bo Bebanto Antipas and Podkapaev Vladimir Michaelovich},
title = {Purification of Staphylococcal Enterotoxin Type D and Obtaining Its Antiserum},
journal = {Animal and Veterinary Sciences},
volume = {3},
number = {6},
pages = {166-170},
doi = {10.11648/j.avs.20150306.14},
url = {https://doi.org/10.11648/j.avs.20150306.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150306.14},
abstract = {Uncontrolled use of antibiotics in the treatment of calves suffering from gastroenteritis and dairy cows suffering from mastitis, created in pathogenic staphylococci enterotoxin producers some resistance to these drugs. These pathogens enterotoxin producers are often excreted with milk from cows with mastitis. That is why the specific and topical issue remains the isolation and identification of strains producing staphylococcal enterotoxin from cow milk and faeces of calves suffering from enteritis. Given all this, we have set a goal to get staphylococcal enterotoxin type D for use as mastitis diagnostic kit and enteritis and antigen for obtaining the antiserum. To obtain the staphylococcal enterotoxin type D we used strain S. aureus No. 494 (ATSS23235) courtesy offered by Dr. FC Fluer from Institute of Experimental Medicine, NF Gamaley, Medical Academy (USSR). Verification of the specificity of the enterotoxin) was performed using 5 strains of S.aureus producing different enterotoxins (entérotoxin- A; enterotoxin-B; enterotoxin C-1; C-2 and enterotoxin - E) as well as non-enterotoxigenic staphylococcal strain (S. aureus Wood 46). In addition to the staphylococcal strains, five strains of Escherichia coli, producing thermostable and thermolabile enterotoxins were also used in the experiment. The method of purification of enterotoxin-D by precipitation twice chromatography on km - cellulose and gel filtration on Sephadex G-75 allows to obtain pure enterotoxin-D having the biological properties, immunological and antigenic characteristics. Immunization of rabbits with purified enterotoxin-D associated with incomplete Freund's adjuvant provides antiserum very active and specific.},
year = {2015}
}
TY - JOUR T1 - Purification of Staphylococcal Enterotoxin Type D and Obtaining Its Antiserum AU - Bidjeh Kebkiba AU - Ban-Bo Bebanto Antipas AU - Podkapaev Vladimir Michaelovich Y1 - 2015/11/03 PY - 2015 N1 - https://doi.org/10.11648/j.avs.20150306.14 DO - 10.11648/j.avs.20150306.14 T2 - Animal and Veterinary Sciences JF - Animal and Veterinary Sciences JO - Animal and Veterinary Sciences SP - 166 EP - 170 PB - Science Publishing Group SN - 2328-5850 UR - https://doi.org/10.11648/j.avs.20150306.14 AB - Uncontrolled use of antibiotics in the treatment of calves suffering from gastroenteritis and dairy cows suffering from mastitis, created in pathogenic staphylococci enterotoxin producers some resistance to these drugs. These pathogens enterotoxin producers are often excreted with milk from cows with mastitis. That is why the specific and topical issue remains the isolation and identification of strains producing staphylococcal enterotoxin from cow milk and faeces of calves suffering from enteritis. Given all this, we have set a goal to get staphylococcal enterotoxin type D for use as mastitis diagnostic kit and enteritis and antigen for obtaining the antiserum. To obtain the staphylococcal enterotoxin type D we used strain S. aureus No. 494 (ATSS23235) courtesy offered by Dr. FC Fluer from Institute of Experimental Medicine, NF Gamaley, Medical Academy (USSR). Verification of the specificity of the enterotoxin) was performed using 5 strains of S.aureus producing different enterotoxins (entérotoxin- A; enterotoxin-B; enterotoxin C-1; C-2 and enterotoxin - E) as well as non-enterotoxigenic staphylococcal strain (S. aureus Wood 46). In addition to the staphylococcal strains, five strains of Escherichia coli, producing thermostable and thermolabile enterotoxins were also used in the experiment. The method of purification of enterotoxin-D by precipitation twice chromatography on km - cellulose and gel filtration on Sephadex G-75 allows to obtain pure enterotoxin-D having the biological properties, immunological and antigenic characteristics. Immunization of rabbits with purified enterotoxin-D associated with incomplete Freund's adjuvant provides antiserum very active and specific. VL - 3 IS - 6 ER -
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