Bioactive Composite Material Based on Polyurethane with Isoniazid

Dmytro Kuliesh; Nataliia Galatenko; Rita Rozhnova; Ludmila Nechaeva

doi:doi:10.11648/j.ajpst.20230901.11

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Bioactive Composite Material Based on Polyurethane with Isoniazid

Dmytro Kuliesh, Nataliia Galatenko, Rita Rozhnova, Ludmila Nechaeva

Published in American Journal of Polymer Science and Technology (Volume 9, Issue 1)

Received: 10 February 2023 Accepted: 11 April 2023 Published: 20 April 2023

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Abstract

An urgent task is the development of new implantation materials with a prolonged medicinal effect for the treatment of the extrapulmonary form of tuberculosis. The purpose of this investigation was to obtain an implantation material based on mesh polyurethane with the anti-tuberculosis drug isoniazid, to study the drug release of isoniazid from the polymer, to study the biodegradation of such materials, as well as to analyze cellular reactions to their implantation in the body of experimental animals. According to the results of the study of the dynamics of the release of isoniazid from the structure of mesh polyurethane, it was shown that a little more than 44% of isoniazid was released from the polymer matrix during the 389 days of the study. At the same time, in the first 3 days, a shock dose of the anti-tuberculosis drug was released – more than 18%, which can be important for reducing the pathological process at the location of the implant. When studying the biodegradation of the obtained materials, it was established that there was a redistribution of supramolecular structures, in which the type of these structures remained unchanged, but the size of the globular particles changed. In the model environments, the organization of large, possibly hydrophobic, destruction fragments into large globular formations probably took place, which was reflected in the increase of some physical and mechanical indicators. During implantation, the polymer material was subjected to cellular resorption due to the activity of phagocytic fragments, as well as mechanical loads, which contributed to the removal of destruction products from the mass of the polymer and led to a decrease in the main physical and mechanical parameters. According to the results of the implantation test by histological methods, it was established that around the samples of polyurethane with isoniazid a rather pronounced and long-lasting (up to 1 month) reaction of the type of aseptic inflammation was observed. At the same time, the cellular composition of the connective tissue capsule indicated a fairly good tolerability of the polymer material with prolonged release of isoniazid. It is shown that the obtained bioactive composite material based on polyurethane with isoniazid can be a promising implant material for the treatment of bone and joint tuberculosis.

Published in	American Journal of Polymer Science and Technology (Volume 9, Issue 1)
DOI	10.11648/j.ajpst.20230901.11
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Composite Material, Polyurethane, Isoniazid, Drug Release, Biodegradation, Implantation

References

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[2]	Galatenko N. A., Kuliesh D. V., Gritsenko V. P., Narazhayko L. F. Influence of the prolonged form of lysocyme in the composition of polyurethane implant on cells and tissues in vitro and in vivo. Morphologia, 2021, 15 (2), 16-24. doi: https://doi.org/10.26641/1997-9665.2021.2.16-24.
[3]	Galatenko N. A., Kulyesh D. V., Maletskyi A. P., Karpenko O. S. Soft-tissue response to synthetic polymer implants made of cross-linked polyurethane and containing a biologically active substance, albucid or dacarbazine, in animals. Journal of Ophthalmology, 2018, № 6, 52-58. http://doi.org/10.31288/oftalmolzh201865258.
[4]	Abdelkader H., Fathalla Z., Seyfoddin A., Farahani M., Thrimawithana T., Allahham A., Alani A., Al-Kinani A., Alany R. Polymeric long-acting drug delivery systems (LADDS) for treatment of chronic diseases: Inserts, patches, wafers, and implants, Advanced Drug Delivery Reviews, Volume 177, 2021, 113957, ISSN 0169-409X. https://doi.org/10.1016/j.addr.2021.113957
[5]	Domingues Goncalves A, Balestri W, Reinwald Y. Biomedical Implants for Regenerative Therapies. Biomaterials, 2020, Nov 11. http://dx.doi.org/10.5772/intechopen.91295
[6]	Kim J. O., Kabanov A. V., Bronich T. K. Polymer micelles with cross-linked polyanion core for delivery of a cationic drug doxorubicin // Journal of Controlled Release, Volume 138, Issue 3, 2009, 197-204. https://doi.org/10.1016/j.jconrel.2009.04.019.
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[9]	Stewart S. A., Domínguez-Robles J., Donnelly R. F., Larrañeta E. Implantable Polymeric Drug Delivery Devices: Classification, Manufacture, Materials, and Clinical Applications. Polymers. 2018; 10 (12), 1379. https://doi.org/10.3390/polym10121379.
[10]	Galatenko N. A., Rozhnova R. A. Biologically active polymeric materials for medicine, Kyiv, Naukova Dumka, 2013, 211 p.
[11]	W. Kenneth Ward, Emily P Slobodzian, Kenneth L Tiekotter, Michael D Wood, The effect of microgeometry, implant thickness and polyurethane chemistry on the foreign body response to subcutaneous implants, Biomaterials, Volume 23, Issue 21, 2002, 4185-4192. https://doi.org/10.1016/S0142-9612(02)00160-6.
[12]	Galatenko N. A., R. A. Rozhnova, Kuliesh D. V., Denisenko V. D., Maletskyy A. P., Bigun N. M. Rat tissue responses to dacarbazine-containing implants made of cross-linked polyurethane of different densities. Journal of Ophthalmology, 2022, 4 (507), 40-48. http://doi.org/10.31288/oftalmolzh202244048.
[13]	Galatenko N. A., Malanchuk V. O., Rozhnova R. A.. Astapenko O. O., Rudenchyk T. V. Biologically active polyurethane compositions for endoprosthesis of bone tissue, Kyiv, Naukova dumka, 2020, 230 p. ISBN 978-966-00-1784-9.
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[16]	Lipatova T. E., Phakadze G. A. Polymers in endoprosthesis, Kyiv, Naukova dumka, 1983, 160 p.

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APA Style

Dmytro Kuliesh, Nataliia Galatenko, Rita Rozhnova, Ludmila Nechaeva. (2023). Bioactive Composite Material Based on Polyurethane with Isoniazid. American Journal of Polymer Science and Technology, 9(1), 1-6. https://doi.org/10.11648/j.ajpst.20230901.11

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ACS Style

Dmytro Kuliesh; Nataliia Galatenko; Rita Rozhnova; Ludmila Nechaeva. Bioactive Composite Material Based on Polyurethane with Isoniazid. Am. J. Polym. Sci. Technol. 2023, 9(1), 1-6. doi: 10.11648/j.ajpst.20230901.11

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AMA Style

Dmytro Kuliesh, Nataliia Galatenko, Rita Rozhnova, Ludmila Nechaeva. Bioactive Composite Material Based on Polyurethane with Isoniazid. Am J Polym Sci Technol. 2023;9(1):1-6. doi: 10.11648/j.ajpst.20230901.11

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@article{10.11648/j.ajpst.20230901.11,
  author = {Dmytro Kuliesh and Nataliia Galatenko and Rita Rozhnova and Ludmila Nechaeva},
  title = {Bioactive Composite Material Based on Polyurethane with Isoniazid},
  journal = {American Journal of Polymer Science and Technology},
  volume = {9},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ajpst.20230901.11},
  url = {https://doi.org/10.11648/j.ajpst.20230901.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpst.20230901.11},
  abstract = {An urgent task is the development of new implantation materials with a prolonged medicinal effect for the treatment of the extrapulmonary form of tuberculosis. The purpose of this investigation was to obtain an implantation material based on mesh polyurethane with the anti-tuberculosis drug isoniazid, to study the drug release of isoniazid from the polymer, to study the biodegradation of such materials, as well as to analyze cellular reactions to their implantation in the body of experimental animals. According to the results of the study of the dynamics of the release of isoniazid from the structure of mesh polyurethane, it was shown that a little more than 44% of isoniazid was released from the polymer matrix during the 389 days of the study. At the same time, in the first 3 days, a shock dose of the anti-tuberculosis drug was released – more than 18%, which can be important for reducing the pathological process at the location of the implant. When studying the biodegradation of the obtained materials, it was established that there was a redistribution of supramolecular structures, in which the type of these structures remained unchanged, but the size of the globular particles changed. In the model environments, the organization of large, possibly hydrophobic, destruction fragments into large globular formations probably took place, which was reflected in the increase of some physical and mechanical indicators. During implantation, the polymer material was subjected to cellular resorption due to the activity of phagocytic fragments, as well as mechanical loads, which contributed to the removal of destruction products from the mass of the polymer and led to a decrease in the main physical and mechanical parameters. According to the results of the implantation test by histological methods, it was established that around the samples of polyurethane with isoniazid a rather pronounced and long-lasting (up to 1 month) reaction of the type of aseptic inflammation was observed. At the same time, the cellular composition of the connective tissue capsule indicated a fairly good tolerability of the polymer material with prolonged release of isoniazid. It is shown that the obtained bioactive composite material based on polyurethane with isoniazid can be a promising implant material for the treatment of bone and joint tuberculosis.},
 year = {2023}
}

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TY  - JOUR
T1  - Bioactive Composite Material Based on Polyurethane with Isoniazid
AU  - Dmytro Kuliesh
AU  - Nataliia Galatenko
AU  - Rita Rozhnova
AU  - Ludmila Nechaeva
Y1  - 2023/04/20
PY  - 2023
N1  - https://doi.org/10.11648/j.ajpst.20230901.11
DO  - 10.11648/j.ajpst.20230901.11
T2  - American Journal of Polymer Science and Technology
JF  - American Journal of Polymer Science and Technology
JO  - American Journal of Polymer Science and Technology
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2575-5986
UR  - https://doi.org/10.11648/j.ajpst.20230901.11
AB  - An urgent task is the development of new implantation materials with a prolonged medicinal effect for the treatment of the extrapulmonary form of tuberculosis. The purpose of this investigation was to obtain an implantation material based on mesh polyurethane with the anti-tuberculosis drug isoniazid, to study the drug release of isoniazid from the polymer, to study the biodegradation of such materials, as well as to analyze cellular reactions to their implantation in the body of experimental animals. According to the results of the study of the dynamics of the release of isoniazid from the structure of mesh polyurethane, it was shown that a little more than 44% of isoniazid was released from the polymer matrix during the 389 days of the study. At the same time, in the first 3 days, a shock dose of the anti-tuberculosis drug was released – more than 18%, which can be important for reducing the pathological process at the location of the implant. When studying the biodegradation of the obtained materials, it was established that there was a redistribution of supramolecular structures, in which the type of these structures remained unchanged, but the size of the globular particles changed. In the model environments, the organization of large, possibly hydrophobic, destruction fragments into large globular formations probably took place, which was reflected in the increase of some physical and mechanical indicators. During implantation, the polymer material was subjected to cellular resorption due to the activity of phagocytic fragments, as well as mechanical loads, which contributed to the removal of destruction products from the mass of the polymer and led to a decrease in the main physical and mechanical parameters. According to the results of the implantation test by histological methods, it was established that around the samples of polyurethane with isoniazid a rather pronounced and long-lasting (up to 1 month) reaction of the type of aseptic inflammation was observed. At the same time, the cellular composition of the connective tissue capsule indicated a fairly good tolerability of the polymer material with prolonged release of isoniazid. It is shown that the obtained bioactive composite material based on polyurethane with isoniazid can be a promising implant material for the treatment of bone and joint tuberculosis.
VL  - 9
IS  - 1
ER  -

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Author Information

Dmytro Kuliesh

Department of Polymers of Medical Appointment, Institute of Macromolecular Chemistry of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Nataliia Galatenko

Department of Polymers of Medical Appointment, Institute of Macromolecular Chemistry of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Rita Rozhnova

Department of Polymers of Medical Appointment, Institute of Macromolecular Chemistry of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
Ludmila Nechaeva

Department of Polymers of Medical Appointment, Institute of Macromolecular Chemistry of the National Academy of Sciences of Ukraine, Kyiv, Ukraine

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APA Style

Dmytro Kuliesh, Nataliia Galatenko, Rita Rozhnova, Ludmila Nechaeva. (2023). Bioactive Composite Material Based on Polyurethane with Isoniazid. American Journal of Polymer Science and Technology, 9(1), 1-6. https://doi.org/10.11648/j.ajpst.20230901.11

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ACS Style

Dmytro Kuliesh; Nataliia Galatenko; Rita Rozhnova; Ludmila Nechaeva. Bioactive Composite Material Based on Polyurethane with Isoniazid. Am. J. Polym. Sci. Technol. 2023, 9(1), 1-6. doi: 10.11648/j.ajpst.20230901.11

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AMA Style

Dmytro Kuliesh, Nataliia Galatenko, Rita Rozhnova, Ludmila Nechaeva. Bioactive Composite Material Based on Polyurethane with Isoniazid. Am J Polym Sci Technol. 2023;9(1):1-6. doi: 10.11648/j.ajpst.20230901.11

Copy | Download

@article{10.11648/j.ajpst.20230901.11,
  author = {Dmytro Kuliesh and Nataliia Galatenko and Rita Rozhnova and Ludmila Nechaeva},
  title = {Bioactive Composite Material Based on Polyurethane with Isoniazid},
  journal = {American Journal of Polymer Science and Technology},
  volume = {9},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ajpst.20230901.11},
  url = {https://doi.org/10.11648/j.ajpst.20230901.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpst.20230901.11},
  abstract = {An urgent task is the development of new implantation materials with a prolonged medicinal effect for the treatment of the extrapulmonary form of tuberculosis. The purpose of this investigation was to obtain an implantation material based on mesh polyurethane with the anti-tuberculosis drug isoniazid, to study the drug release of isoniazid from the polymer, to study the biodegradation of such materials, as well as to analyze cellular reactions to their implantation in the body of experimental animals. According to the results of the study of the dynamics of the release of isoniazid from the structure of mesh polyurethane, it was shown that a little more than 44% of isoniazid was released from the polymer matrix during the 389 days of the study. At the same time, in the first 3 days, a shock dose of the anti-tuberculosis drug was released – more than 18%, which can be important for reducing the pathological process at the location of the implant. When studying the biodegradation of the obtained materials, it was established that there was a redistribution of supramolecular structures, in which the type of these structures remained unchanged, but the size of the globular particles changed. In the model environments, the organization of large, possibly hydrophobic, destruction fragments into large globular formations probably took place, which was reflected in the increase of some physical and mechanical indicators. During implantation, the polymer material was subjected to cellular resorption due to the activity of phagocytic fragments, as well as mechanical loads, which contributed to the removal of destruction products from the mass of the polymer and led to a decrease in the main physical and mechanical parameters. According to the results of the implantation test by histological methods, it was established that around the samples of polyurethane with isoniazid a rather pronounced and long-lasting (up to 1 month) reaction of the type of aseptic inflammation was observed. At the same time, the cellular composition of the connective tissue capsule indicated a fairly good tolerability of the polymer material with prolonged release of isoniazid. It is shown that the obtained bioactive composite material based on polyurethane with isoniazid can be a promising implant material for the treatment of bone and joint tuberculosis.},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Bioactive Composite Material Based on Polyurethane with Isoniazid
AU  - Dmytro Kuliesh
AU  - Nataliia Galatenko
AU  - Rita Rozhnova
AU  - Ludmila Nechaeva
Y1  - 2023/04/20
PY  - 2023
N1  - https://doi.org/10.11648/j.ajpst.20230901.11
DO  - 10.11648/j.ajpst.20230901.11
T2  - American Journal of Polymer Science and Technology
JF  - American Journal of Polymer Science and Technology
JO  - American Journal of Polymer Science and Technology
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2575-5986
UR  - https://doi.org/10.11648/j.ajpst.20230901.11
AB  - An urgent task is the development of new implantation materials with a prolonged medicinal effect for the treatment of the extrapulmonary form of tuberculosis. The purpose of this investigation was to obtain an implantation material based on mesh polyurethane with the anti-tuberculosis drug isoniazid, to study the drug release of isoniazid from the polymer, to study the biodegradation of such materials, as well as to analyze cellular reactions to their implantation in the body of experimental animals. According to the results of the study of the dynamics of the release of isoniazid from the structure of mesh polyurethane, it was shown that a little more than 44% of isoniazid was released from the polymer matrix during the 389 days of the study. At the same time, in the first 3 days, a shock dose of the anti-tuberculosis drug was released – more than 18%, which can be important for reducing the pathological process at the location of the implant. When studying the biodegradation of the obtained materials, it was established that there was a redistribution of supramolecular structures, in which the type of these structures remained unchanged, but the size of the globular particles changed. In the model environments, the organization of large, possibly hydrophobic, destruction fragments into large globular formations probably took place, which was reflected in the increase of some physical and mechanical indicators. During implantation, the polymer material was subjected to cellular resorption due to the activity of phagocytic fragments, as well as mechanical loads, which contributed to the removal of destruction products from the mass of the polymer and led to a decrease in the main physical and mechanical parameters. According to the results of the implantation test by histological methods, it was established that around the samples of polyurethane with isoniazid a rather pronounced and long-lasting (up to 1 month) reaction of the type of aseptic inflammation was observed. At the same time, the cellular composition of the connective tissue capsule indicated a fairly good tolerability of the polymer material with prolonged release of isoniazid. It is shown that the obtained bioactive composite material based on polyurethane with isoniazid can be a promising implant material for the treatment of bone and joint tuberculosis.
VL  - 9
IS  - 1
ER  -

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