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Effect of Pseudomonas Fluorescens as Biocontrol of Rice Blight Disease Cause by Curvularia Lunata on Rice Variety Gawal R1

Received: 16 August 2025     Accepted: 28 August 2025     Published: 24 December 2025
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Abstract

Curvularia lunata is a soil-borne fungal pathogen that attacks rice and causes significant losses in its production, while Pseudomonas fluorescens is one of the common biocontrol agents used for managing foliar infection in soil born pathogen. The aim of this research was to study the effect of Pseudomonas fluorescens as biocontrol agent against Curvularia lunata on rice variety Gawal r1. The isolate were tested for pathogenicity. In a pot experiment, P. fluorescens significantly reduced the disease severity of rice blight caused by C. lunata. After application of a spore suspension of Pseudomonas fluorescens -biofungicide and Synthetic fungicide (Mancozeb) to rice seedlings inoculated with C. lunata, the disease severity was reduced by 2.00, 2.55, 3.2, and 4.66, respectively. In Screen house trial, the concentrations Pseudomonas fluorescens concentrations at 3.0 × 108 CFU/ml gave the best results in all plant parameters, as compared to the inoculated control with C. lunata.

Published in American Journal of Plant Biology (Volume 10, Issue 4)
DOI 10.11648/j.ajpb.20251004.12
Page(s) 87-93
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2025. Published by Science Publishing Group

Keywords

Biocontrol, Pseudomonas Fluorescens, Curvularia Lunata, Rice

References
[1] Awoderu, V. A., Bangura, N. and John, V. T. (1991) Incidence, distribution and severity of bacterial diseases on rice in West Africa. Tropical Pest Management 37, 113-117.
[2] American plant health instructor (2007). Rice blast.
[3] Bashir, Y., Sani, Z., Ashafa, A., Mijinyawa, A., Sufiyanu, S., and Abdulazeez, U. (2024). Morphological,
[4] Biochemical and Molecular Characterization of the Causal Agent of Bacterial Panicle Blight Disease of Rice (BPB) In Selected Rice Production Zones of Zamfara State. International Journal of Science for Global Sustainability, 10(2), 138-145.
[5] FAOSTAT. (2018). FAO Statistical databases. Food and Agriculture Organization (FAO) of the United Nations, Rome.
[6] Ganeshan, G. and Kumar, A. M. (2005). Pseudomonas fluorescens, a potential bacterial antagonist to control plant diseases. Journal of Plant Interactions, 1(3), 123-134.
[7] Garcia F. O., and C. W. Rice. 1994. Soil Science Society of America Journal 58: 816-823.
[8] Gnanamanickam, S. S. (Ed.) (2009). Biological control of rice diseases, p. 78-89.
[9] Holt, J., G., Krieg, N. R., Sneath, P. H. A., Staley, J. T., And Williams. S. T., (2000). Bergey’s manual of determinative bacteriology, 9th ed., Williams and wilkins, Baltimore. Maryland, Austral journal biotechnology, pp. 787.
[10] Kamaluddeen, Simon, S., and Lal, A. A. (2013). A new blight disease of rice caused by Curvularia lunata from Uttar Pradesh. International Journal of Agricultural Science and Research, 3(5), 13-16.
[11] Kumar, a., gopalakrishnan, s., singh, a. K., rathour, r. And singh, u. D., 2015. Phenotyping of Improved lines and landraces for blast and sheath blight resistance. Indian j. Genet., 74: 499-501
[12] Maeder, p., fliessbach, a., dubois, d., gunst, l., fried, p., & niggli, u. (2002). Soil fertility and Biodiversity in organic farming. Science, 296(5573), 1694-1697.
[13] Majeed Shahid Ashfaq, R. A.,, A. A.,, M., Saleem, M. Z. & Haider, M. S. 2015. First Report of Curvularia lunata causing Brown Leaf Spots of Rice in Punjab, Pakistan
[14] Meena. R. Radhajeyalakshmi, T. Marimuthu, P. Vidhyasekaran and R. Velazhahan (2002), Biological Control of Groundnut Late Leaf Spot and Rust by Seed and Foliar Applications of a Powder Formulation of Pseudomonas fluorescens. Biocontrol Science and Technology, 12: 2, 195-204.
[15] Noori, m. s. s., saud, h. m., and azizi, e.(2015). evaluation of plant growth promoting Rhizobacteria As biocontrol agents for the control of blast disease in rice journal of agricultural science and engineering vol. 1, no. 3, 2015, pp. 135-142.
[16] Ramanathan, A., Shanmugam, V., Raghuchander, T., and Samiyappan, R., (2002). Induction of systemic resistance in ragi against blast disease by pseudomonas fluorescens. America Plant Protection Science. 20, 313-318.
[17] Sen S, Saha A. and Acharya K. 2005. In vitro inhibition of Fusarium oxysporum by florescent Pseudomonas BRL-1. J Bot Soc Beng 59: 31-36.
[18] Singh, rajvir and a. p. Singh (2005). Management of sheath blight by pseudomonas fluorescens. Ann. Pl. Protec. Sci. 13: 410-414.
[19] Sivanesan, A. 1987: Graminicolous species of Bipolaris, Curvularia, Drechslera, Exserohilum and their teleomorphs. Mycological Papers. 158.
[20] Stanhill, g. (1990). The comparative productivity of organic agriculture. Agriculture, ecosystems & environment, 30(1-2), 1-26.
[21] Soytong, k., kanokmedhakul, s., kukongviriyapa, v., & isobe, m. (2001). Application of Chaetomium species (ketomium®) as a new broad spectrum biological fungicide for plant disease control: a review article. Fungal diversity, 7, 1-15.
[22] Woo PC, (2010) Agar block smear preparation: a novel method of slide preparation for preservation of native fungal structures for microscopic examination and long-term storage. J Clin Microbiol 48(9): 3053-61.
[23] Zibaee, A. 2013. Rice: Importance and Future. Journal of Rice Research 1: 2.
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  • APA Style

    Muhammad, Y. A. (2025). Effect of Pseudomonas Fluorescens as Biocontrol of Rice Blight Disease Cause by Curvularia Lunata on Rice Variety Gawal R1. American Journal of Plant Biology, 10(4), 87-93. https://doi.org/10.11648/j.ajpb.20251004.12

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    ACS Style

    Muhammad, Y. A. Effect of Pseudomonas Fluorescens as Biocontrol of Rice Blight Disease Cause by Curvularia Lunata on Rice Variety Gawal R1. Am. J. Plant Biol. 2025, 10(4), 87-93. doi: 10.11648/j.ajpb.20251004.12

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    AMA Style

    Muhammad YA. Effect of Pseudomonas Fluorescens as Biocontrol of Rice Blight Disease Cause by Curvularia Lunata on Rice Variety Gawal R1. Am J Plant Biol. 2025;10(4):87-93. doi: 10.11648/j.ajpb.20251004.12

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  • @article{10.11648/j.ajpb.20251004.12,
      author = {Yabagi Abdullahi Muhammad},
      title = {Effect of Pseudomonas Fluorescens as Biocontrol of Rice Blight Disease Cause by Curvularia Lunata on Rice Variety Gawal R1},
      journal = {American Journal of Plant Biology},
      volume = {10},
      number = {4},
      pages = {87-93},
      doi = {10.11648/j.ajpb.20251004.12},
      url = {https://doi.org/10.11648/j.ajpb.20251004.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20251004.12},
      abstract = {Curvularia lunata is a soil-borne fungal pathogen that attacks rice and causes significant losses in its production, while Pseudomonas fluorescens is one of the common biocontrol agents used for managing foliar infection in soil born pathogen. The aim of this research was to study the effect of Pseudomonas fluorescens as biocontrol agent against Curvularia lunata on rice variety Gawal r1. The isolate were tested for pathogenicity. In a pot experiment, P. fluorescens significantly reduced the disease severity of rice blight caused by C. lunata. After application of a spore suspension of Pseudomonas fluorescens -biofungicide and Synthetic fungicide (Mancozeb) to rice seedlings inoculated with C. lunata, the disease severity was reduced by 2.00, 2.55, 3.2, and 4.66, respectively. In Screen house trial, the concentrations Pseudomonas fluorescens concentrations at 3.0 × 108 CFU/ml gave the best results in all plant parameters, as compared to the inoculated control with C. lunata.},
     year = {2025}
    }
    

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    T1  - Effect of Pseudomonas Fluorescens as Biocontrol of Rice Blight Disease Cause by Curvularia Lunata on Rice Variety Gawal R1
    AU  - Yabagi Abdullahi Muhammad
    Y1  - 2025/12/24
    PY  - 2025
    N1  - https://doi.org/10.11648/j.ajpb.20251004.12
    DO  - 10.11648/j.ajpb.20251004.12
    T2  - American Journal of Plant Biology
    JF  - American Journal of Plant Biology
    JO  - American Journal of Plant Biology
    SP  - 87
    EP  - 93
    PB  - Science Publishing Group
    SN  - 2578-8337
    UR  - https://doi.org/10.11648/j.ajpb.20251004.12
    AB  - Curvularia lunata is a soil-borne fungal pathogen that attacks rice and causes significant losses in its production, while Pseudomonas fluorescens is one of the common biocontrol agents used for managing foliar infection in soil born pathogen. The aim of this research was to study the effect of Pseudomonas fluorescens as biocontrol agent against Curvularia lunata on rice variety Gawal r1. The isolate were tested for pathogenicity. In a pot experiment, P. fluorescens significantly reduced the disease severity of rice blight caused by C. lunata. After application of a spore suspension of Pseudomonas fluorescens -biofungicide and Synthetic fungicide (Mancozeb) to rice seedlings inoculated with C. lunata, the disease severity was reduced by 2.00, 2.55, 3.2, and 4.66, respectively. In Screen house trial, the concentrations Pseudomonas fluorescens concentrations at 3.0 × 108 CFU/ml gave the best results in all plant parameters, as compared to the inoculated control with C. lunata.
    VL  - 10
    IS  - 4
    ER  - 

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Author Information
  • Department of Plant Science and Biotechnology, Bayero University, Kano, Nigeria

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