Relationship of MTB Positive Cases with CD4+ Count and Qualitative Leucocytes and Values of Erythrocyte Sedimentation Rate in MTB Positive Cases in Gombe State

Doughari James Hamuel; Ja’afaru Mohammed Inuwa; Luka Joseph

doi:doi:10.11648/j.ajlm.20220703.11

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Relationship of MTB Positive Cases with CD4+ Count and Qualitative Leucocytes and Values of Erythrocyte Sedimentation Rate in MTB Positive Cases in Gombe State

Doughari James Hamuel, Ja’afaru Mohammed Inuwa, Luka Joseph

Published in American Journal of Laboratory Medicine (Volume 7, Issue 3)

Received: 1 May 2022 Accepted: 24 May 2022 Published: 28 July 2022

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Abstract

Tuberculosis caused by Mycobacterium tuberculosis (M. tuberculosis) bacteria [link.springer.com] remains deadliest communicable disease. In this study the relationship of MTB positive cases with CD4+ count quantitative and qualitative leucocytes and values of erythrocyte sedimentation rate (ESR) in MTB positive cases of patients with suspected pulmonary tuberculosis in Gombe State, Nigeria was determined. Four hundred (400) sputum and blood samples were collected from tuberculosis suspects referred to the laboratory from TB clinics in three Senatorial Zones of Gombe State. The sputum samples were analyzed using GeneXpert MTB/RIF and culture on MGIT tubes and LJ slants methods. The CD4+count determination was carried out using BD FACSCOUNT flow cytometery automated machine (BD Biosciences, 2007 Germany). Complete blood count determination was carried out using 5 - Part SYSMEX XN-330 HAEMATOLOGY ANALYZER (SYSMEX CORPORATION 2017). Result from this study showed Males have higher percentage MTB positivity cases than the females. However, both males and female within the age groups 25 to 54 years showed high rate of MTB positive cases. Mean CD₄₊ count of MTB positive patients obtained in this study was significantly lower (337.0 ±1.4) than that of the apparently healthy control (753.6 ±1.28). In this study the mean Haemoglobin (Hb) values (9.8 ±3.12 g/dL) among TB patients were lower than those the control group (13.7±2.71 g/dL) for both males and females. Result from this study also showed that the mean neutrophil count value (55.1 ±13.8) of infected patients was higher than that of the healthy control group (46.3 ±22.19) and the mean lymphocytes count value (34.7 ±13.5) of infected patients was lower compared to healthy control group value (42.13 ±20.59). Report from this study showed high proportion of MTB positive patients in Gombe State have subnormal CD4+ count cells, lymphocytopenia, anaemia, neutropenia, thrombocytosis, leucocytosis and high erythrocyte sedimentation rate level before starting treatment.

Published in	American Journal of Laboratory Medicine (Volume 7, Issue 3)
DOI	10.11648/j.ajlm.20220703.11
Page(s)	37-42
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

GeneXpert, Mycobacterium tuberculoisis, CD4+ Count, Haematology Parameters, Sputum, Culture

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APA Style

Doughari James Hamuel, Ja’afaru Mohammed Inuwa, Luka Joseph. (2022). Relationship of MTB Positive Cases with CD4+ Count and Qualitative Leucocytes and Values of Erythrocyte Sedimentation Rate in MTB Positive Cases in Gombe State. American Journal of Laboratory Medicine, 7(3), 37-42. https://doi.org/10.11648/j.ajlm.20220703.11

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ACS Style

Doughari James Hamuel; Ja’afaru Mohammed Inuwa; Luka Joseph. Relationship of MTB Positive Cases with CD4+ Count and Qualitative Leucocytes and Values of Erythrocyte Sedimentation Rate in MTB Positive Cases in Gombe State. Am. J. Lab. Med. 2022, 7(3), 37-42. doi: 10.11648/j.ajlm.20220703.11

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AMA Style

Doughari James Hamuel, Ja’afaru Mohammed Inuwa, Luka Joseph. Relationship of MTB Positive Cases with CD4+ Count and Qualitative Leucocytes and Values of Erythrocyte Sedimentation Rate in MTB Positive Cases in Gombe State. Am J Lab Med. 2022;7(3):37-42. doi: 10.11648/j.ajlm.20220703.11

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@article{10.11648/j.ajlm.20220703.11,
  author = {Doughari James Hamuel and Ja’afaru Mohammed Inuwa and Luka Joseph},
  title = {Relationship of MTB Positive Cases with CD4+ Count and Qualitative Leucocytes and Values of Erythrocyte Sedimentation Rate in MTB Positive Cases in Gombe State},
  journal = {American Journal of Laboratory Medicine},
  volume = {7},
  number = {3},
  pages = {37-42},
  doi = {10.11648/j.ajlm.20220703.11},
  url = {https://doi.org/10.11648/j.ajlm.20220703.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20220703.11},
  abstract = {Tuberculosis caused by Mycobacterium tuberculosis (M. tuberculosis) bacteria [link.springer.com] remains deadliest communicable disease. In this study the relationship of MTB positive cases with CD4+ count quantitative and qualitative leucocytes and values of erythrocyte sedimentation rate (ESR) in MTB positive cases of patients with suspected pulmonary tuberculosis in Gombe State, Nigeria was determined. Four hundred (400) sputum and blood samples were collected from tuberculosis suspects referred to the laboratory from TB clinics in three Senatorial Zones of Gombe State. The sputum samples were analyzed using GeneXpert MTB/RIF and culture on MGIT tubes and LJ slants methods. The CD4+count determination was carried out using BD FACSCOUNT flow cytometery automated machine (BD Biosciences, 2007 Germany). Complete blood count determination was carried out using 5 - Part SYSMEX XN-330 HAEMATOLOGY ANALYZER (SYSMEX CORPORATION 2017). Result from this study showed Males have higher percentage MTB positivity cases than the females. However, both males and female within the age groups 25 to 54 years showed high rate of MTB positive cases. Mean CD4+ count of MTB positive patients obtained in this study was significantly lower (337.0 ±1.4) than that of the apparently healthy control (753.6 ±1.28). In this study the mean Haemoglobin (Hb) values (9.8 ±3.12 g/dL) among TB patients were lower than those the control group (13.7±2.71 g/dL) for both males and females. Result from this study also showed that the mean neutrophil count value (55.1 ±13.8) of infected patients was higher than that of the healthy control group (46.3 ±22.19) and the mean lymphocytes count value (34.7 ±13.5) of infected patients was lower compared to healthy control group value (42.13 ±20.59). Report from this study showed high proportion of MTB positive patients in Gombe State have subnormal CD4+ count cells, lymphocytopenia, anaemia, neutropenia, thrombocytosis, leucocytosis and high erythrocyte sedimentation rate level before starting treatment.},
 year = {2022}
}

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TY  - JOUR
T1  - Relationship of MTB Positive Cases with CD4+ Count and Qualitative Leucocytes and Values of Erythrocyte Sedimentation Rate in MTB Positive Cases in Gombe State
AU  - Doughari James Hamuel
AU  - Ja’afaru Mohammed Inuwa
AU  - Luka Joseph
Y1  - 2022/07/28
PY  - 2022
N1  - https://doi.org/10.11648/j.ajlm.20220703.11
DO  - 10.11648/j.ajlm.20220703.11
T2  - American Journal of Laboratory Medicine
JF  - American Journal of Laboratory Medicine
JO  - American Journal of Laboratory Medicine
SP  - 37
EP  - 42
PB  - Science Publishing Group
SN  - 2575-386X
UR  - https://doi.org/10.11648/j.ajlm.20220703.11
AB  - Tuberculosis caused by Mycobacterium tuberculosis (M. tuberculosis) bacteria [link.springer.com] remains deadliest communicable disease. In this study the relationship of MTB positive cases with CD4+ count quantitative and qualitative leucocytes and values of erythrocyte sedimentation rate (ESR) in MTB positive cases of patients with suspected pulmonary tuberculosis in Gombe State, Nigeria was determined. Four hundred (400) sputum and blood samples were collected from tuberculosis suspects referred to the laboratory from TB clinics in three Senatorial Zones of Gombe State. The sputum samples were analyzed using GeneXpert MTB/RIF and culture on MGIT tubes and LJ slants methods. The CD4+count determination was carried out using BD FACSCOUNT flow cytometery automated machine (BD Biosciences, 2007 Germany). Complete blood count determination was carried out using 5 - Part SYSMEX XN-330 HAEMATOLOGY ANALYZER (SYSMEX CORPORATION 2017). Result from this study showed Males have higher percentage MTB positivity cases than the females. However, both males and female within the age groups 25 to 54 years showed high rate of MTB positive cases. Mean CD4+ count of MTB positive patients obtained in this study was significantly lower (337.0 ±1.4) than that of the apparently healthy control (753.6 ±1.28). In this study the mean Haemoglobin (Hb) values (9.8 ±3.12 g/dL) among TB patients were lower than those the control group (13.7±2.71 g/dL) for both males and females. Result from this study also showed that the mean neutrophil count value (55.1 ±13.8) of infected patients was higher than that of the healthy control group (46.3 ±22.19) and the mean lymphocytes count value (34.7 ±13.5) of infected patients was lower compared to healthy control group value (42.13 ±20.59). Report from this study showed high proportion of MTB positive patients in Gombe State have subnormal CD4+ count cells, lymphocytopenia, anaemia, neutropenia, thrombocytosis, leucocytosis and high erythrocyte sedimentation rate level before starting treatment.
VL  - 7
IS  - 3
ER  -

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Author Information

Doughari James Hamuel

Department of Microbiology, Modibbo Adama University, Yola, Nigeria
Ja’afaru Mohammed Inuwa

Department of Microbiology, Modibbo Adama University, Yola, Nigeria
Luka Joseph

Diagnosis Department, Gombe State Ministry of Health, Gombe, Nigeria

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APA Style

Doughari James Hamuel, Ja’afaru Mohammed Inuwa, Luka Joseph. (2022). Relationship of MTB Positive Cases with CD4+ Count and Qualitative Leucocytes and Values of Erythrocyte Sedimentation Rate in MTB Positive Cases in Gombe State. American Journal of Laboratory Medicine, 7(3), 37-42. https://doi.org/10.11648/j.ajlm.20220703.11

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ACS Style

Doughari James Hamuel; Ja’afaru Mohammed Inuwa; Luka Joseph. Relationship of MTB Positive Cases with CD4+ Count and Qualitative Leucocytes and Values of Erythrocyte Sedimentation Rate in MTB Positive Cases in Gombe State. Am. J. Lab. Med. 2022, 7(3), 37-42. doi: 10.11648/j.ajlm.20220703.11

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AMA Style

Doughari James Hamuel, Ja’afaru Mohammed Inuwa, Luka Joseph. Relationship of MTB Positive Cases with CD4+ Count and Qualitative Leucocytes and Values of Erythrocyte Sedimentation Rate in MTB Positive Cases in Gombe State. Am J Lab Med. 2022;7(3):37-42. doi: 10.11648/j.ajlm.20220703.11

Copy | Download

@article{10.11648/j.ajlm.20220703.11,
  author = {Doughari James Hamuel and Ja’afaru Mohammed Inuwa and Luka Joseph},
  title = {Relationship of MTB Positive Cases with CD4+ Count and Qualitative Leucocytes and Values of Erythrocyte Sedimentation Rate in MTB Positive Cases in Gombe State},
  journal = {American Journal of Laboratory Medicine},
  volume = {7},
  number = {3},
  pages = {37-42},
  doi = {10.11648/j.ajlm.20220703.11},
  url = {https://doi.org/10.11648/j.ajlm.20220703.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20220703.11},
  abstract = {Tuberculosis caused by Mycobacterium tuberculosis (M. tuberculosis) bacteria [link.springer.com] remains deadliest communicable disease. In this study the relationship of MTB positive cases with CD4+ count quantitative and qualitative leucocytes and values of erythrocyte sedimentation rate (ESR) in MTB positive cases of patients with suspected pulmonary tuberculosis in Gombe State, Nigeria was determined. Four hundred (400) sputum and blood samples were collected from tuberculosis suspects referred to the laboratory from TB clinics in three Senatorial Zones of Gombe State. The sputum samples were analyzed using GeneXpert MTB/RIF and culture on MGIT tubes and LJ slants methods. The CD4+count determination was carried out using BD FACSCOUNT flow cytometery automated machine (BD Biosciences, 2007 Germany). Complete blood count determination was carried out using 5 - Part SYSMEX XN-330 HAEMATOLOGY ANALYZER (SYSMEX CORPORATION 2017). Result from this study showed Males have higher percentage MTB positivity cases than the females. However, both males and female within the age groups 25 to 54 years showed high rate of MTB positive cases. Mean CD4+ count of MTB positive patients obtained in this study was significantly lower (337.0 ±1.4) than that of the apparently healthy control (753.6 ±1.28). In this study the mean Haemoglobin (Hb) values (9.8 ±3.12 g/dL) among TB patients were lower than those the control group (13.7±2.71 g/dL) for both males and females. Result from this study also showed that the mean neutrophil count value (55.1 ±13.8) of infected patients was higher than that of the healthy control group (46.3 ±22.19) and the mean lymphocytes count value (34.7 ±13.5) of infected patients was lower compared to healthy control group value (42.13 ±20.59). Report from this study showed high proportion of MTB positive patients in Gombe State have subnormal CD4+ count cells, lymphocytopenia, anaemia, neutropenia, thrombocytosis, leucocytosis and high erythrocyte sedimentation rate level before starting treatment.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Relationship of MTB Positive Cases with CD4+ Count and Qualitative Leucocytes and Values of Erythrocyte Sedimentation Rate in MTB Positive Cases in Gombe State
AU  - Doughari James Hamuel
AU  - Ja’afaru Mohammed Inuwa
AU  - Luka Joseph
Y1  - 2022/07/28
PY  - 2022
N1  - https://doi.org/10.11648/j.ajlm.20220703.11
DO  - 10.11648/j.ajlm.20220703.11
T2  - American Journal of Laboratory Medicine
JF  - American Journal of Laboratory Medicine
JO  - American Journal of Laboratory Medicine
SP  - 37
EP  - 42
PB  - Science Publishing Group
SN  - 2575-386X
UR  - https://doi.org/10.11648/j.ajlm.20220703.11
AB  - Tuberculosis caused by Mycobacterium tuberculosis (M. tuberculosis) bacteria [link.springer.com] remains deadliest communicable disease. In this study the relationship of MTB positive cases with CD4+ count quantitative and qualitative leucocytes and values of erythrocyte sedimentation rate (ESR) in MTB positive cases of patients with suspected pulmonary tuberculosis in Gombe State, Nigeria was determined. Four hundred (400) sputum and blood samples were collected from tuberculosis suspects referred to the laboratory from TB clinics in three Senatorial Zones of Gombe State. The sputum samples were analyzed using GeneXpert MTB/RIF and culture on MGIT tubes and LJ slants methods. The CD4+count determination was carried out using BD FACSCOUNT flow cytometery automated machine (BD Biosciences, 2007 Germany). Complete blood count determination was carried out using 5 - Part SYSMEX XN-330 HAEMATOLOGY ANALYZER (SYSMEX CORPORATION 2017). Result from this study showed Males have higher percentage MTB positivity cases than the females. However, both males and female within the age groups 25 to 54 years showed high rate of MTB positive cases. Mean CD4+ count of MTB positive patients obtained in this study was significantly lower (337.0 ±1.4) than that of the apparently healthy control (753.6 ±1.28). In this study the mean Haemoglobin (Hb) values (9.8 ±3.12 g/dL) among TB patients were lower than those the control group (13.7±2.71 g/dL) for both males and females. Result from this study also showed that the mean neutrophil count value (55.1 ±13.8) of infected patients was higher than that of the healthy control group (46.3 ±22.19) and the mean lymphocytes count value (34.7 ±13.5) of infected patients was lower compared to healthy control group value (42.13 ±20.59). Report from this study showed high proportion of MTB positive patients in Gombe State have subnormal CD4+ count cells, lymphocytopenia, anaemia, neutropenia, thrombocytosis, leucocytosis and high erythrocyte sedimentation rate level before starting treatment.
VL  - 7
IS  - 3
ER  -

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