Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting

Hiroshani Hansamali Kulatunga; Ajith Aloka Pathirana; Neluka Fernando; Bawantha Gamage; Apsara Epa; Asanga Sampath; Cherine Sosai; Bimalka Seneviratne

doi:doi:10.11648/j.ajlm.20200506.13

Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting

Hiroshani Hansamali Kulatunga, Ajith Aloka Pathirana, Neluka Fernando, Bawantha Gamage, Apsara Epa, Asanga Sampath, Cherine Sosai, Bimalka Seneviratne

Published in American Journal of Laboratory Medicine (Volume 5, Issue 6)

Received: 4 November 2020 Accepted: 27 November 2020 Published: 4 December 2020

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Abstract

Over the past thirty years thyroid tumor incidence has triplicated and it is currently 7% of a given population. However, interestingly the thyroid cancer incidence is quite low and is approximately 5-10% of the thyroid tumor patients. Expeditious diagnosis of the thyroid nodules at the pre surgery stage is important to determine the tumor prognosis. A Widely used diagnostic approach is the direct observation of the fine needle aspiration specimens. However, using only this method many reported cases of unnecessary surgeries and re-operative incidents due to false diagnosis at the preoperative stage have been observed. Poor cellularity, loss of tissue architecture, poor cytomorphologic features in fine needle aspiration smears leads to incorrect diagnosis. Cell block technique is a better diagnostic approach at the pre-operative stage to overcome the pitfalls in direct smears as it concentrates the cells and preserve tissue architecture. Further, multiple sections with same cellularity can be prepared from a single cell block allowing the use for more advanced techniques like immunohistochemistry and molecular diagnosis. Cell block technique strengthen the diagnosis of tumors by fine needle aspiration specimens. Combined use of cell block technique with fine needle aspiration can reach to a sensitivity of about 95-100% suggesting that every FNAC specimen should be subjected to cell block preparation whenever possible.

Published in	American Journal of Laboratory Medicine (Volume 5, Issue 6)
DOI	10.11648/j.ajlm.20200506.13
Page(s)	167-173
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Cell Block, Fine Needle Aspiration Cytology, Thyroid Tumors, Immunohistochemistry

References

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APA Style

Hiroshani Hansamali Kulatunga, Ajith Aloka Pathirana, Neluka Fernando, Bawantha Gamage, Apsara Epa, et al. (2020). Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting. American Journal of Laboratory Medicine, 5(6), 167-173. https://doi.org/10.11648/j.ajlm.20200506.13

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ACS Style

Hiroshani Hansamali Kulatunga; Ajith Aloka Pathirana; Neluka Fernando; Bawantha Gamage; Apsara Epa, et al. Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting. Am. J. Lab. Med. 2020, 5(6), 167-173. doi: 10.11648/j.ajlm.20200506.13

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AMA Style

Hiroshani Hansamali Kulatunga, Ajith Aloka Pathirana, Neluka Fernando, Bawantha Gamage, Apsara Epa, et al. Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting. Am J Lab Med. 2020;5(6):167-173. doi: 10.11648/j.ajlm.20200506.13

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@article{10.11648/j.ajlm.20200506.13,
  author = {Hiroshani Hansamali Kulatunga and Ajith Aloka Pathirana and Neluka Fernando and Bawantha Gamage and Apsara Epa and Asanga Sampath and Cherine Sosai and Bimalka Seneviratne},
  title = {Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting},
  journal = {American Journal of Laboratory Medicine},
  volume = {5},
  number = {6},
  pages = {167-173},
  doi = {10.11648/j.ajlm.20200506.13},
  url = {https://doi.org/10.11648/j.ajlm.20200506.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20200506.13},
  abstract = {Over the past thirty years thyroid tumor incidence has triplicated and it is currently 7% of a given population. However, interestingly the thyroid cancer incidence is quite low and is approximately 5-10% of the thyroid tumor patients. Expeditious diagnosis of the thyroid nodules at the pre surgery stage is important to determine the tumor prognosis. A Widely used diagnostic approach is the direct observation of the fine needle aspiration specimens. However, using only this method many reported cases of unnecessary surgeries and re-operative incidents due to false diagnosis at the preoperative stage have been observed. Poor cellularity, loss of tissue architecture, poor cytomorphologic features in fine needle aspiration smears leads to incorrect diagnosis. Cell block technique is a better diagnostic approach at the pre-operative stage to overcome the pitfalls in direct smears as it concentrates the cells and preserve tissue architecture. Further, multiple sections with same cellularity can be prepared from a single cell block allowing the use for more advanced techniques like immunohistochemistry and molecular diagnosis. Cell block technique strengthen the diagnosis of tumors by fine needle aspiration specimens. Combined use of cell block technique with fine needle aspiration can reach to a sensitivity of about 95-100% suggesting that every FNAC specimen should be subjected to cell block preparation whenever possible.},
 year = {2020}
}

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TY  - JOUR
T1  - Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting
AU  - Hiroshani Hansamali Kulatunga
AU  - Ajith Aloka Pathirana
AU  - Neluka Fernando
AU  - Bawantha Gamage
AU  - Apsara Epa
AU  - Asanga Sampath
AU  - Cherine Sosai
AU  - Bimalka Seneviratne
Y1  - 2020/12/04
PY  - 2020
N1  - https://doi.org/10.11648/j.ajlm.20200506.13
DO  - 10.11648/j.ajlm.20200506.13
T2  - American Journal of Laboratory Medicine
JF  - American Journal of Laboratory Medicine
JO  - American Journal of Laboratory Medicine
SP  - 167
EP  - 173
PB  - Science Publishing Group
SN  - 2575-386X
UR  - https://doi.org/10.11648/j.ajlm.20200506.13
AB  - Over the past thirty years thyroid tumor incidence has triplicated and it is currently 7% of a given population. However, interestingly the thyroid cancer incidence is quite low and is approximately 5-10% of the thyroid tumor patients. Expeditious diagnosis of the thyroid nodules at the pre surgery stage is important to determine the tumor prognosis. A Widely used diagnostic approach is the direct observation of the fine needle aspiration specimens. However, using only this method many reported cases of unnecessary surgeries and re-operative incidents due to false diagnosis at the preoperative stage have been observed. Poor cellularity, loss of tissue architecture, poor cytomorphologic features in fine needle aspiration smears leads to incorrect diagnosis. Cell block technique is a better diagnostic approach at the pre-operative stage to overcome the pitfalls in direct smears as it concentrates the cells and preserve tissue architecture. Further, multiple sections with same cellularity can be prepared from a single cell block allowing the use for more advanced techniques like immunohistochemistry and molecular diagnosis. Cell block technique strengthen the diagnosis of tumors by fine needle aspiration specimens. Combined use of cell block technique with fine needle aspiration can reach to a sensitivity of about 95-100% suggesting that every FNAC specimen should be subjected to cell block preparation whenever possible.
VL  - 5
IS  - 6
ER  -

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Author Information

Hiroshani Hansamali Kulatunga

Department of Bio Medical Science, Kaatsu International University, Colombo, Sri Lanka
Ajith Aloka Pathirana

Department of Surgery, University of Sri Jayewardenepura, Colombo, Sri Lanka
Neluka Fernando

Department of Microbiology, University of Sri Jayewardenepura, Colombo, Sri Lanka
Bawantha Gamage

Department of Surgery, University of Sri Jayewardenepura, Colombo, Sri Lanka
Apsara Epa

Department of Radiology, Colombo South Teaching Hospital, Kalubowila, Sri Lanka
Asanga Sampath

Department of Basic Science, University of Sri Jayewardenepura, Colombo, Sri Lanka
Cherine Sosai

Department of Pathology, Colombo South Teaching Hospital, Kalubowila, Sri Lanka
Bimalka Seneviratne

Department of Pathology, University of Sri Jayewardenepura, Colombo, Sri Lanka

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APA Style

Hiroshani Hansamali Kulatunga, Ajith Aloka Pathirana, Neluka Fernando, Bawantha Gamage, Apsara Epa, et al. (2020). Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting. American Journal of Laboratory Medicine, 5(6), 167-173. https://doi.org/10.11648/j.ajlm.20200506.13

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ACS Style

Hiroshani Hansamali Kulatunga; Ajith Aloka Pathirana; Neluka Fernando; Bawantha Gamage; Apsara Epa, et al. Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting. Am. J. Lab. Med. 2020, 5(6), 167-173. doi: 10.11648/j.ajlm.20200506.13

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AMA Style

Hiroshani Hansamali Kulatunga, Ajith Aloka Pathirana, Neluka Fernando, Bawantha Gamage, Apsara Epa, et al. Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting. Am J Lab Med. 2020;5(6):167-173. doi: 10.11648/j.ajlm.20200506.13

Copy | Download

@article{10.11648/j.ajlm.20200506.13,
  author = {Hiroshani Hansamali Kulatunga and Ajith Aloka Pathirana and Neluka Fernando and Bawantha Gamage and Apsara Epa and Asanga Sampath and Cherine Sosai and Bimalka Seneviratne},
  title = {Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting},
  journal = {American Journal of Laboratory Medicine},
  volume = {5},
  number = {6},
  pages = {167-173},
  doi = {10.11648/j.ajlm.20200506.13},
  url = {https://doi.org/10.11648/j.ajlm.20200506.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20200506.13},
  abstract = {Over the past thirty years thyroid tumor incidence has triplicated and it is currently 7% of a given population. However, interestingly the thyroid cancer incidence is quite low and is approximately 5-10% of the thyroid tumor patients. Expeditious diagnosis of the thyroid nodules at the pre surgery stage is important to determine the tumor prognosis. A Widely used diagnostic approach is the direct observation of the fine needle aspiration specimens. However, using only this method many reported cases of unnecessary surgeries and re-operative incidents due to false diagnosis at the preoperative stage have been observed. Poor cellularity, loss of tissue architecture, poor cytomorphologic features in fine needle aspiration smears leads to incorrect diagnosis. Cell block technique is a better diagnostic approach at the pre-operative stage to overcome the pitfalls in direct smears as it concentrates the cells and preserve tissue architecture. Further, multiple sections with same cellularity can be prepared from a single cell block allowing the use for more advanced techniques like immunohistochemistry and molecular diagnosis. Cell block technique strengthen the diagnosis of tumors by fine needle aspiration specimens. Combined use of cell block technique with fine needle aspiration can reach to a sensitivity of about 95-100% suggesting that every FNAC specimen should be subjected to cell block preparation whenever possible.},
 year = {2020}
}

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TY  - JOUR
T1  - Cell Block Preparation for Fine Needle Aspiration Cytology of Thyroid; Cost Effective Method in a Resource Poor Setting
AU  - Hiroshani Hansamali Kulatunga
AU  - Ajith Aloka Pathirana
AU  - Neluka Fernando
AU  - Bawantha Gamage
AU  - Apsara Epa
AU  - Asanga Sampath
AU  - Cherine Sosai
AU  - Bimalka Seneviratne
Y1  - 2020/12/04
PY  - 2020
N1  - https://doi.org/10.11648/j.ajlm.20200506.13
DO  - 10.11648/j.ajlm.20200506.13
T2  - American Journal of Laboratory Medicine
JF  - American Journal of Laboratory Medicine
JO  - American Journal of Laboratory Medicine
SP  - 167
EP  - 173
PB  - Science Publishing Group
SN  - 2575-386X
UR  - https://doi.org/10.11648/j.ajlm.20200506.13
AB  - Over the past thirty years thyroid tumor incidence has triplicated and it is currently 7% of a given population. However, interestingly the thyroid cancer incidence is quite low and is approximately 5-10% of the thyroid tumor patients. Expeditious diagnosis of the thyroid nodules at the pre surgery stage is important to determine the tumor prognosis. A Widely used diagnostic approach is the direct observation of the fine needle aspiration specimens. However, using only this method many reported cases of unnecessary surgeries and re-operative incidents due to false diagnosis at the preoperative stage have been observed. Poor cellularity, loss of tissue architecture, poor cytomorphologic features in fine needle aspiration smears leads to incorrect diagnosis. Cell block technique is a better diagnostic approach at the pre-operative stage to overcome the pitfalls in direct smears as it concentrates the cells and preserve tissue architecture. Further, multiple sections with same cellularity can be prepared from a single cell block allowing the use for more advanced techniques like immunohistochemistry and molecular diagnosis. Cell block technique strengthen the diagnosis of tumors by fine needle aspiration specimens. Combined use of cell block technique with fine needle aspiration can reach to a sensitivity of about 95-100% suggesting that every FNAC specimen should be subjected to cell block preparation whenever possible.
VL  - 5
IS  - 6
ER  -

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