The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria

Faustina Nkechi Osuji; Charles Chinedum Onyenekwe; Nkeiruka Rose Ukaibe; Joseph Ebere Ahaneku

doi:doi:10.11648/j.ajlm.20180306.12

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The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria

Faustina Nkechi Osuji, Charles Chinedum Onyenekwe, Nkeiruka Rose Ukaibe, Joseph Ebere Ahaneku

Published in American Journal of Laboratory Medicine (Volume 3, Issue 6)

Received: 26 May 2018 Accepted: 25 December 2018 Published: 17 January 2019

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Abstract

The study determined the effects of highly active antiretroviral therapy on the levels of Superoxide dismutase, catalase and C-reactive protein HIV-infected subjects in Nigeria. A total of 50 HIV infected subjects aged 20-69 (39±10) years and 50 HIV seronegative control participantsaged 21-56 (35±10) years were recruited for the study. Blood samples were collected at 3 different points: before initiation of HAART, 6 months and 12 months into HAART. The serum levels of C - reactive protein (CRP), catalase, Superoxide Dismutase (SOD), CD4 + T cells and viral load counts were measured in these subjects before HAART initiation and at 6 and 12 months after HAART intake. Standard laboratory methods were used in the analysis of these parameters. The results showed that CRP was significantly increased in HIV infected subjects before commencement of HAART and remained significantly increased after 12 months intake of HAART compared to control participants (P<0.01)respectively. SOD, and CD4 were significantly lower before HAART initiation and after 12 months intake of HAART compared to control participants (P<0.01) respectively. Viral load was significantly reduced after 12 months intake of HAART. There was a negative correlation between the viral load and SOD (r=-0.41, P<0.01) and catalase (r=-0.47, P<0.01) and a positive correlation between the viral load and CRP (r=0.48, P<0.01) before HAART initiation. After 12 months of HAART catalase showed a significant negative correlation with viral load (r=-0.37, P<0.05) while CRP showed a positive correlation with viral load (r=0.33, P<0.05). The study shows a persistently elevated CRP and reduced SOD and catalase after 12 months intake of HAART. These biomarkers support a central role of inflammation and oxidative stress in HIV pathogenesis.

Published in	American Journal of Laboratory Medicine (Volume 3, Issue 6)
DOI	10.11648/j.ajlm.20180306.12
Page(s)	53-59
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Highly Active Antiretroviral Therapy, HIV, Catalase, SOD, C - Reactive Protein

References

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APA Style

Faustina Nkechi Osuji, Charles Chinedum Onyenekwe, Nkeiruka Rose Ukaibe, Joseph Ebere Ahaneku. (2019). The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria. American Journal of Laboratory Medicine, 3(6), 53-59. https://doi.org/10.11648/j.ajlm.20180306.12

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ACS Style

Faustina Nkechi Osuji; Charles Chinedum Onyenekwe; Nkeiruka Rose Ukaibe; Joseph Ebere Ahaneku. The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria. Am. J. Lab. Med. 2019, 3(6), 53-59. doi: 10.11648/j.ajlm.20180306.12

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AMA Style

Faustina Nkechi Osuji, Charles Chinedum Onyenekwe, Nkeiruka Rose Ukaibe, Joseph Ebere Ahaneku. The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria. Am J Lab Med. 2019;3(6):53-59. doi: 10.11648/j.ajlm.20180306.12

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@article{10.11648/j.ajlm.20180306.12,
  author = {Faustina Nkechi Osuji and Charles Chinedum Onyenekwe and Nkeiruka Rose Ukaibe and Joseph Ebere Ahaneku},
  title = {The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria},
  journal = {American Journal of Laboratory Medicine},
  volume = {3},
  number = {6},
  pages = {53-59},
  doi = {10.11648/j.ajlm.20180306.12},
  url = {https://doi.org/10.11648/j.ajlm.20180306.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20180306.12},
  abstract = {The study determined the effects of highly active antiretroviral therapy on the levels of Superoxide dismutase, catalase and C-reactive protein HIV-infected subjects in Nigeria. A total of 50 HIV infected subjects aged 20-69 (39±10) years and 50 HIV seronegative control participantsaged 21-56 (35±10) years were recruited for the study. Blood samples were collected at 3 different points: before initiation of HAART, 6 months and 12 months into HAART. The serum levels of C - reactive protein (CRP), catalase, Superoxide Dismutase (SOD), CD4 + T cells and viral load counts were measured in these subjects before HAART initiation and at 6 and 12 months after HAART intake. Standard laboratory methods were used in the analysis of these parameters. The results showed that CRP was significantly increased in HIV infected subjects before commencement of HAART and remained significantly increased after 12 months intake of HAART compared to control participants (P<0.01)respectively. SOD, and CD4 were significantly lower before HAART initiation and after 12 months intake of HAART compared to control participants (P<0.01) respectively. Viral load was significantly reduced after 12 months intake of HAART. There was a negative correlation between the viral load and SOD (r=-0.41, P<0.01) and catalase (r=-0.47, P<0.01) and a positive correlation between the viral load and CRP (r=0.48, P<0.01) before HAART initiation. After 12 months of HAART catalase showed a significant negative correlation with viral load (r=-0.37, P<0.05) while CRP showed a positive correlation with viral load (r=0.33, P<0.05). The study shows a persistently elevated CRP and reduced SOD and catalase after 12 months intake of HAART. These biomarkers support a central role of inflammation and oxidative stress in HIV pathogenesis.},
 year = {2019}
}

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TY  - JOUR
T1  - The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria
AU  - Faustina Nkechi Osuji
AU  - Charles Chinedum Onyenekwe
AU  - Nkeiruka Rose Ukaibe
AU  - Joseph Ebere Ahaneku
Y1  - 2019/01/17
PY  - 2019
N1  - https://doi.org/10.11648/j.ajlm.20180306.12
DO  - 10.11648/j.ajlm.20180306.12
T2  - American Journal of Laboratory Medicine
JF  - American Journal of Laboratory Medicine
JO  - American Journal of Laboratory Medicine
SP  - 53
EP  - 59
PB  - Science Publishing Group
SN  - 2575-386X
UR  - https://doi.org/10.11648/j.ajlm.20180306.12
AB  - The study determined the effects of highly active antiretroviral therapy on the levels of Superoxide dismutase, catalase and C-reactive protein HIV-infected subjects in Nigeria. A total of 50 HIV infected subjects aged 20-69 (39±10) years and 50 HIV seronegative control participantsaged 21-56 (35±10) years were recruited for the study. Blood samples were collected at 3 different points: before initiation of HAART, 6 months and 12 months into HAART. The serum levels of C - reactive protein (CRP), catalase, Superoxide Dismutase (SOD), CD4 + T cells and viral load counts were measured in these subjects before HAART initiation and at 6 and 12 months after HAART intake. Standard laboratory methods were used in the analysis of these parameters. The results showed that CRP was significantly increased in HIV infected subjects before commencement of HAART and remained significantly increased after 12 months intake of HAART compared to control participants (P<0.01)respectively. SOD, and CD4 were significantly lower before HAART initiation and after 12 months intake of HAART compared to control participants (P<0.01) respectively. Viral load was significantly reduced after 12 months intake of HAART. There was a negative correlation between the viral load and SOD (r=-0.41, P<0.01) and catalase (r=-0.47, P<0.01) and a positive correlation between the viral load and CRP (r=0.48, P<0.01) before HAART initiation. After 12 months of HAART catalase showed a significant negative correlation with viral load (r=-0.37, P<0.05) while CRP showed a positive correlation with viral load (r=0.33, P<0.05). The study shows a persistently elevated CRP and reduced SOD and catalase after 12 months intake of HAART. These biomarkers support a central role of inflammation and oxidative stress in HIV pathogenesis.
VL  - 3
IS  - 6
ER  -

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Author Information

Faustina Nkechi Osuji

Laboratory Department, Immaculate Heart Specialist Hospital, Nkpor-Agu, Nigeria
Charles Chinedum Onyenekwe

Department of Medical Laboratory Science, NnamdiAzikiwe University, Nnewi Campus, Nigeria
Nkeiruka Rose Ukaibe

Department of Medical Laboratory Science, NnamdiAzikiwe University, Nnewi Campus, Nigeria
Joseph Ebere Ahaneku

Department of Chemical Pathology, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria

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APA Style

Faustina Nkechi Osuji, Charles Chinedum Onyenekwe, Nkeiruka Rose Ukaibe, Joseph Ebere Ahaneku. (2019). The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria. American Journal of Laboratory Medicine, 3(6), 53-59. https://doi.org/10.11648/j.ajlm.20180306.12

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ACS Style

Faustina Nkechi Osuji; Charles Chinedum Onyenekwe; Nkeiruka Rose Ukaibe; Joseph Ebere Ahaneku. The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria. Am. J. Lab. Med. 2019, 3(6), 53-59. doi: 10.11648/j.ajlm.20180306.12

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AMA Style

Faustina Nkechi Osuji, Charles Chinedum Onyenekwe, Nkeiruka Rose Ukaibe, Joseph Ebere Ahaneku. The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria. Am J Lab Med. 2019;3(6):53-59. doi: 10.11648/j.ajlm.20180306.12

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@article{10.11648/j.ajlm.20180306.12,
  author = {Faustina Nkechi Osuji and Charles Chinedum Onyenekwe and Nkeiruka Rose Ukaibe and Joseph Ebere Ahaneku},
  title = {The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria},
  journal = {American Journal of Laboratory Medicine},
  volume = {3},
  number = {6},
  pages = {53-59},
  doi = {10.11648/j.ajlm.20180306.12},
  url = {https://doi.org/10.11648/j.ajlm.20180306.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20180306.12},
  abstract = {The study determined the effects of highly active antiretroviral therapy on the levels of Superoxide dismutase, catalase and C-reactive protein HIV-infected subjects in Nigeria. A total of 50 HIV infected subjects aged 20-69 (39±10) years and 50 HIV seronegative control participantsaged 21-56 (35±10) years were recruited for the study. Blood samples were collected at 3 different points: before initiation of HAART, 6 months and 12 months into HAART. The serum levels of C - reactive protein (CRP), catalase, Superoxide Dismutase (SOD), CD4 + T cells and viral load counts were measured in these subjects before HAART initiation and at 6 and 12 months after HAART intake. Standard laboratory methods were used in the analysis of these parameters. The results showed that CRP was significantly increased in HIV infected subjects before commencement of HAART and remained significantly increased after 12 months intake of HAART compared to control participants (P<0.01)respectively. SOD, and CD4 were significantly lower before HAART initiation and after 12 months intake of HAART compared to control participants (P<0.01) respectively. Viral load was significantly reduced after 12 months intake of HAART. There was a negative correlation between the viral load and SOD (r=-0.41, P<0.01) and catalase (r=-0.47, P<0.01) and a positive correlation between the viral load and CRP (r=0.48, P<0.01) before HAART initiation. After 12 months of HAART catalase showed a significant negative correlation with viral load (r=-0.37, P<0.05) while CRP showed a positive correlation with viral load (r=0.33, P<0.05). The study shows a persistently elevated CRP and reduced SOD and catalase after 12 months intake of HAART. These biomarkers support a central role of inflammation and oxidative stress in HIV pathogenesis.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - The Effects of Highly Active Antiretroviral Therapy on the Levels of Superoxide Dismutase, Catalase and C-Reactive Proteinin HIV Infected Subjects in Nigeria
AU  - Faustina Nkechi Osuji
AU  - Charles Chinedum Onyenekwe
AU  - Nkeiruka Rose Ukaibe
AU  - Joseph Ebere Ahaneku
Y1  - 2019/01/17
PY  - 2019
N1  - https://doi.org/10.11648/j.ajlm.20180306.12
DO  - 10.11648/j.ajlm.20180306.12
T2  - American Journal of Laboratory Medicine
JF  - American Journal of Laboratory Medicine
JO  - American Journal of Laboratory Medicine
SP  - 53
EP  - 59
PB  - Science Publishing Group
SN  - 2575-386X
UR  - https://doi.org/10.11648/j.ajlm.20180306.12
AB  - The study determined the effects of highly active antiretroviral therapy on the levels of Superoxide dismutase, catalase and C-reactive protein HIV-infected subjects in Nigeria. A total of 50 HIV infected subjects aged 20-69 (39±10) years and 50 HIV seronegative control participantsaged 21-56 (35±10) years were recruited for the study. Blood samples were collected at 3 different points: before initiation of HAART, 6 months and 12 months into HAART. The serum levels of C - reactive protein (CRP), catalase, Superoxide Dismutase (SOD), CD4 + T cells and viral load counts were measured in these subjects before HAART initiation and at 6 and 12 months after HAART intake. Standard laboratory methods were used in the analysis of these parameters. The results showed that CRP was significantly increased in HIV infected subjects before commencement of HAART and remained significantly increased after 12 months intake of HAART compared to control participants (P<0.01)respectively. SOD, and CD4 were significantly lower before HAART initiation and after 12 months intake of HAART compared to control participants (P<0.01) respectively. Viral load was significantly reduced after 12 months intake of HAART. There was a negative correlation between the viral load and SOD (r=-0.41, P<0.01) and catalase (r=-0.47, P<0.01) and a positive correlation between the viral load and CRP (r=0.48, P<0.01) before HAART initiation. After 12 months of HAART catalase showed a significant negative correlation with viral load (r=-0.37, P<0.05) while CRP showed a positive correlation with viral load (r=0.33, P<0.05). The study shows a persistently elevated CRP and reduced SOD and catalase after 12 months intake of HAART. These biomarkers support a central role of inflammation and oxidative stress in HIV pathogenesis.
VL  - 3
IS  - 6
ER  -

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