Molecular Study of NPM1-A (Nucleophosmin1-A) Mutation in Iraqi Adult Acute Myeloid Leukemia Patients; Its Correlation with Clinicopathological Parameters

Shaymaa Abdulateef; Ali Almothaffar; Khitam Razzak Al-khafaji

doi:doi:10.11648/j.ajim.20170503.11

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Molecular Study of NPM1-A (Nucleophosmin1-A) Mutation in Iraqi Adult Acute Myeloid Leukemia Patients; Its Correlation with Clinicopathological Parameters

Shaymaa Abdulateef, Ali Almothaffar, Khitam Razzak Al-khafaji

Published in American Journal of Internal Medicine (Volume 5, Issue 3)

Received: 3 March 2017 Accepted: 20 March 2017 Published: 19 April 2017

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Abstract

Acute myeloid leukemia (AML) is a hematological malignancy of myeloid progenitor cells characterized by anomalous proliferation, inhibition of differentiation and expansion of leukemic cells blocked at the early stage of hematopoiesis. The molecular markers have become a smart tool to further division of AML patients into subgroups. Nucleophosmin (NPM1) mutations are found in approximately 30% of adult AML patients and are associated with a favorable outcome when detected in absence of FLT3-ITD mutation. Over 50 molecular NPM1 mutation variants have been identified; the most common one is NPM1-A mutation. The current study aims to detect; the frequency of NPM1-A in Iraqi adult newly diagnosed AML patients using real time – PCR technique and evaluate the relationship of NPM1-A with age, gender, total white blood cell (WBC) count and FAB subtypes of the disease. The frequency of NPM1-A mutation in newly diagnosed AML patients was 18.86%. Age was non-significantly higher in NPM1-A mutated patients than in patients without mutation (P=0.538). The NPM1-A mutation was predominantly seen in male (P= 0.069). The mutation was non-significantly higher among monocytic subtypes (M5+M4) compared with the other subtypes of the disease (P=0.916). In the NPM1 mutated group, the total WBC count was non-significantly higher than the non-mutated group (P=0.302). These findings suggest; Real time-PCR technique using TaqMan probe was very specific molecular test for this mutation. Mutated NPM1-A patients were associated with increased age, a higher total WBC count, male predominance and the monocytic subtypes of the disease.

Published in	American Journal of Internal Medicine (Volume 5, Issue 3)
DOI	10.11648/j.ajim.20170503.11
Page(s)	37-40
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Acute Myeloid Leukemia, NPM1-A, Real Time-PCR

References

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APA Style

Shaymaa Abdulateef, Ali Almothaffar, Khitam Razzak Al-khafaji. (2017). Molecular Study of NPM1-A (Nucleophosmin1-A) Mutation in Iraqi Adult Acute Myeloid Leukemia Patients; Its Correlation with Clinicopathological Parameters. American Journal of Internal Medicine, 5(3), 37-40. https://doi.org/10.11648/j.ajim.20170503.11

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ACS Style

Shaymaa Abdulateef; Ali Almothaffar; Khitam Razzak Al-khafaji. Molecular Study of NPM1-A (Nucleophosmin1-A) Mutation in Iraqi Adult Acute Myeloid Leukemia Patients; Its Correlation with Clinicopathological Parameters. Am. J. Intern. Med. 2017, 5(3), 37-40. doi: 10.11648/j.ajim.20170503.11

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AMA Style

Shaymaa Abdulateef, Ali Almothaffar, Khitam Razzak Al-khafaji. Molecular Study of NPM1-A (Nucleophosmin1-A) Mutation in Iraqi Adult Acute Myeloid Leukemia Patients; Its Correlation with Clinicopathological Parameters. Am J Intern Med. 2017;5(3):37-40. doi: 10.11648/j.ajim.20170503.11

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@article{10.11648/j.ajim.20170503.11,
  author = {Shaymaa Abdulateef and Ali Almothaffar and Khitam Razzak Al-khafaji},
  title = {Molecular Study of NPM1-A (Nucleophosmin1-A) Mutation in Iraqi Adult Acute Myeloid Leukemia Patients; Its Correlation with Clinicopathological Parameters},
  journal = {American Journal of Internal Medicine},
  volume = {5},
  number = {3},
  pages = {37-40},
  doi = {10.11648/j.ajim.20170503.11},
  url = {https://doi.org/10.11648/j.ajim.20170503.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20170503.11},
  abstract = {Acute myeloid leukemia (AML) is a hematological malignancy of myeloid progenitor cells characterized by anomalous proliferation, inhibition of differentiation and expansion of leukemic cells blocked at the early stage of hematopoiesis. The molecular markers have become a smart tool to further division of AML patients into subgroups. Nucleophosmin (NPM1) mutations are found in approximately 30% of adult AML patients and are associated with a favorable outcome when detected in absence of FLT3-ITD mutation. Over 50 molecular NPM1 mutation variants have been identified; the most common one is NPM1-A mutation. The current study aims to detect; the frequency of NPM1-A in Iraqi adult newly diagnosed AML patients using real time – PCR technique and evaluate the relationship of NPM1-A with age, gender, total white blood cell (WBC) count and FAB subtypes of the disease. The frequency of NPM1-A mutation in newly diagnosed AML patients was 18.86%. Age was non-significantly higher in NPM1-A mutated patients than in patients without mutation (P=0.538). The NPM1-A mutation was predominantly seen in male (P= 0.069). The mutation was non-significantly higher among monocytic subtypes (M5+M4) compared with the other subtypes of the disease (P=0.916). In the NPM1 mutated group, the total WBC count was non-significantly higher than the non-mutated group (P=0.302). These findings suggest; Real time-PCR technique using TaqMan probe was very specific molecular test for this mutation. Mutated NPM1-A patients were associated with increased age, a higher total WBC count, male predominance and the monocytic subtypes of the disease.},
 year = {2017}
}

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TY - JOUR
T1 - Molecular Study of NPM1-A (Nucleophosmin1-A) Mutation in Iraqi Adult Acute Myeloid Leukemia Patients; Its Correlation with Clinicopathological Parameters
AU - Shaymaa Abdulateef
AU - Ali Almothaffar
AU - Khitam Razzak Al-khafaji
Y1 - 2017/04/19
PY - 2017
N1 - https://doi.org/10.11648/j.ajim.20170503.11
DO - 10.11648/j.ajim.20170503.11
T2 - American Journal of Internal Medicine
JF - American Journal of Internal Medicine
JO - American Journal of Internal Medicine
SP - 37
EP - 40
PB - Science Publishing Group
SN - 2330-4324
UR - https://doi.org/10.11648/j.ajim.20170503.11
AB - Acute myeloid leukemia (AML) is a hematological malignancy of myeloid progenitor cells characterized by anomalous proliferation, inhibition of differentiation and expansion of leukemic cells blocked at the early stage of hematopoiesis. The molecular markers have become a smart tool to further division of AML patients into subgroups. Nucleophosmin (NPM1) mutations are found in approximately 30% of adult AML patients and are associated with a favorable outcome when detected in absence of FLT3-ITD mutation. Over 50 molecular NPM1 mutation variants have been identified; the most common one is NPM1-A mutation. The current study aims to detect; the frequency of NPM1-A in Iraqi adult newly diagnosed AML patients using real time – PCR technique and evaluate the relationship of NPM1-A with age, gender, total white blood cell (WBC) count and FAB subtypes of the disease. The frequency of NPM1-A mutation in newly diagnosed AML patients was 18.86%. Age was non-significantly higher in NPM1-A mutated patients than in patients without mutation (P=0.538). The NPM1-A mutation was predominantly seen in male (P= 0.069). The mutation was non-significantly higher among monocytic subtypes (M5+M4) compared with the other subtypes of the disease (P=0.916). In the NPM1 mutated group, the total WBC count was non-significantly higher than the non-mutated group (P=0.302). These findings suggest; Real time-PCR technique using TaqMan probe was very specific molecular test for this mutation. Mutated NPM1-A patients were associated with increased age, a higher total WBC count, male predominance and the monocytic subtypes of the disease.
VL - 5
IS - 3
ER -

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Author Information

Shaymaa Abdulateef

Baquba Teaching Hospital, Diyala, Iraq
Ali Almothaffar

Department of Medicine, University of Baghdad, Baghdad, Iraq
Khitam Razzak Al-khafaji

Department of Pathology and Forensic Medicine, University of Baghdad, Baghdad, Iraq

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APA Style

Shaymaa Abdulateef, Ali Almothaffar, Khitam Razzak Al-khafaji. (2017). Molecular Study of NPM1-A (Nucleophosmin1-A) Mutation in Iraqi Adult Acute Myeloid Leukemia Patients; Its Correlation with Clinicopathological Parameters. American Journal of Internal Medicine, 5(3), 37-40. https://doi.org/10.11648/j.ajim.20170503.11

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ACS Style

Shaymaa Abdulateef; Ali Almothaffar; Khitam Razzak Al-khafaji. Molecular Study of NPM1-A (Nucleophosmin1-A) Mutation in Iraqi Adult Acute Myeloid Leukemia Patients; Its Correlation with Clinicopathological Parameters. Am. J. Intern. Med. 2017, 5(3), 37-40. doi: 10.11648/j.ajim.20170503.11

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AMA Style

Shaymaa Abdulateef, Ali Almothaffar, Khitam Razzak Al-khafaji. Molecular Study of NPM1-A (Nucleophosmin1-A) Mutation in Iraqi Adult Acute Myeloid Leukemia Patients; Its Correlation with Clinicopathological Parameters. Am J Intern Med. 2017;5(3):37-40. doi: 10.11648/j.ajim.20170503.11

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@article{10.11648/j.ajim.20170503.11,
  author = {Shaymaa Abdulateef and Ali Almothaffar and Khitam Razzak Al-khafaji},
  title = {Molecular Study of NPM1-A (Nucleophosmin1-A) Mutation in Iraqi Adult Acute Myeloid Leukemia Patients; Its Correlation with Clinicopathological Parameters},
  journal = {American Journal of Internal Medicine},
  volume = {5},
  number = {3},
  pages = {37-40},
  doi = {10.11648/j.ajim.20170503.11},
  url = {https://doi.org/10.11648/j.ajim.20170503.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20170503.11},
  abstract = {Acute myeloid leukemia (AML) is a hematological malignancy of myeloid progenitor cells characterized by anomalous proliferation, inhibition of differentiation and expansion of leukemic cells blocked at the early stage of hematopoiesis. The molecular markers have become a smart tool to further division of AML patients into subgroups. Nucleophosmin (NPM1) mutations are found in approximately 30% of adult AML patients and are associated with a favorable outcome when detected in absence of FLT3-ITD mutation. Over 50 molecular NPM1 mutation variants have been identified; the most common one is NPM1-A mutation. The current study aims to detect; the frequency of NPM1-A in Iraqi adult newly diagnosed AML patients using real time – PCR technique and evaluate the relationship of NPM1-A with age, gender, total white blood cell (WBC) count and FAB subtypes of the disease. The frequency of NPM1-A mutation in newly diagnosed AML patients was 18.86%. Age was non-significantly higher in NPM1-A mutated patients than in patients without mutation (P=0.538). The NPM1-A mutation was predominantly seen in male (P= 0.069). The mutation was non-significantly higher among monocytic subtypes (M5+M4) compared with the other subtypes of the disease (P=0.916). In the NPM1 mutated group, the total WBC count was non-significantly higher than the non-mutated group (P=0.302). These findings suggest; Real time-PCR technique using TaqMan probe was very specific molecular test for this mutation. Mutated NPM1-A patients were associated with increased age, a higher total WBC count, male predominance and the monocytic subtypes of the disease.},
 year = {2017}
}

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