Research on Polymorphism Relationship of Th17/Treg Cell and Its Factors with STAT4 Gene in AITD Patients with Different Iodine Nutritional Status

Feng Jing; Mu Zhaoxin; Hou Zhenjiang; Wang Cuicui; Li Xinsheng; Chen Yunxia; Liu Jianfeng; Ma Jinqun; Liu Chunyan

doi:doi:10.11648/j.ajcem.20210903.11

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Research on Polymorphism Relationship of Th17/Treg Cell and Its Factors with STAT4 Gene in AITD Patients with Different Iodine Nutritional Status

Feng Jing, Mu Zhaoxin, Hou Zhenjiang, Wang Cuicui, Li Xinsheng, Chen Yunxia, Liu Jianfeng, Ma Jinqun, Liu Chunyan

Published in American Journal of Clinical and Experimental Medicine (Volume 9, Issue 3)

Received: 2 April 2021 Published: 24 May 2021

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Abstract

Background: Research has shown that STAT gene SNP is related to the pathogenesis of T1D, SLE, RA and AITD. Objective: To investigate the correlation of the SNP of Th17/Treg cell and its cytokines with STAT4 in the PBMC of AITD patients with different iodine nutritious status. Method: 100 GD and HT patients who are selected from Cangzhou Central Hospital and Cangzhou People’s Hospital from September 2019 to August 2020, respectively, 60 healthy cases, adopt flow cytometer and quantitative real-time PCR to test the Th17and Treg cell ratio and proportion in PBMC and the expression level of ROR-γt and 3 (Foxp3) mRNA, and use ELISA method to test IL-17 and TGF-β level in the serum. Utilize SASP-PCR technology to test SNP locus (rs7574865) of STAT4 gene, and calculate the genotype and allele frequency. Result: There is some correlation between Th17/Treg cell & its factors in PBMC in the AITD patients and STAT4 rs7574865; compared with healthy control group, the difference between STAT4 rs7574865 G/T &T/T genotype and G&T allele frequency is of statistic significance (χ2 is 6.128 and 5.613 respectively, and the average value of P is <0.05), and may increase the occurrence risk of AITD, but has no correlation with iodine nutritious status. Conclusion: AITD patients not only have Th17 and Treg cell imbalance and the corresponding factor changes but also correlate to STAT4 rs7574865 susceptibility, and the cellular immunity and gene SNP may participate in the disease occurrence process of AIDT together.

Published in	American Journal of Clinical and Experimental Medicine (Volume 9, Issue 3)
DOI	10.11648/j.ajcem.20210903.11
Page(s)	44-54
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Different Iodine Nutritional Status, Graves' Disease, Hashimoto Thyroiditis, Th17/Treg Cells and Factors, STAT4 Genes Polymorphism

References

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APA Style

Feng Jing, Mu Zhaoxin, Hou Zhenjiang, Wang Cuicui, Li Xinsheng, et al. (2021). Research on Polymorphism Relationship of Th17/Treg Cell and Its Factors with STAT4 Gene in AITD Patients with Different Iodine Nutritional Status. American Journal of Clinical and Experimental Medicine, 9(3), 44-54. https://doi.org/10.11648/j.ajcem.20210903.11

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ACS Style

Feng Jing; Mu Zhaoxin; Hou Zhenjiang; Wang Cuicui; Li Xinsheng, et al. Research on Polymorphism Relationship of Th17/Treg Cell and Its Factors with STAT4 Gene in AITD Patients with Different Iodine Nutritional Status. Am. J. Clin. Exp. Med. 2021, 9(3), 44-54. doi: 10.11648/j.ajcem.20210903.11

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AMA Style

Feng Jing, Mu Zhaoxin, Hou Zhenjiang, Wang Cuicui, Li Xinsheng, et al. Research on Polymorphism Relationship of Th17/Treg Cell and Its Factors with STAT4 Gene in AITD Patients with Different Iodine Nutritional Status. Am J Clin Exp Med. 2021;9(3):44-54. doi: 10.11648/j.ajcem.20210903.11

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@article{10.11648/j.ajcem.20210903.11,
  author = {Feng Jing and Mu Zhaoxin and Hou Zhenjiang and Wang Cuicui and Li Xinsheng and Chen Yunxia and Liu Jianfeng and Ma Jinqun and Liu Chunyan},
  title = {Research on Polymorphism Relationship of Th17/Treg Cell and Its Factors with STAT4 Gene in AITD Patients with Different Iodine Nutritional Status},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {9},
  number = {3},
  pages = {44-54},
  doi = {10.11648/j.ajcem.20210903.11},
  url = {https://doi.org/10.11648/j.ajcem.20210903.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20210903.11},
  abstract = {Background: Research has shown that STAT gene SNP is related to the pathogenesis of T1D, SLE, RA and AITD. Objective: To investigate the correlation of the SNP of Th17/Treg cell and its cytokines with STAT4 in the PBMC of AITD patients with different iodine nutritious status. Method: 100 GD and HT patients who are selected from Cangzhou Central Hospital and Cangzhou People’s Hospital from September 2019 to August 2020, respectively, 60 healthy cases, adopt flow cytometer and quantitative real-time PCR to test the Th17and Treg cell ratio and proportion in PBMC and the expression level of ROR-γt and 3 (Foxp3) mRNA, and use ELISA method to test IL-17 and TGF-β level in the serum. Utilize SASP-PCR technology to test SNP locus (rs7574865) of STAT4 gene, and calculate the genotype and allele frequency. Result: There is some correlation between Th17/Treg cell & its factors in PBMC in the AITD patients and STAT4 rs7574865; compared with healthy control group, the difference between STAT4 rs7574865 G/T &T/T genotype and G&T allele frequency is of statistic significance (χ2 is 6.128 and 5.613 respectively, and the average value of P is <0.05), and may increase the occurrence risk of AITD, but has no correlation with iodine nutritious status. Conclusion: AITD patients not only have Th17 and Treg cell imbalance and the corresponding factor changes but also correlate to STAT4 rs7574865 susceptibility, and the cellular immunity and gene SNP may participate in the disease occurrence process of AIDT together.},
 year = {2021}
}

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TY  - JOUR
T1  - Research on Polymorphism Relationship of Th17/Treg Cell and Its Factors with STAT4 Gene in AITD Patients with Different Iodine Nutritional Status
AU  - Feng Jing
AU  - Mu Zhaoxin
AU  - Hou Zhenjiang
AU  - Wang Cuicui
AU  - Li Xinsheng
AU  - Chen Yunxia
AU  - Liu Jianfeng
AU  - Ma Jinqun
AU  - Liu Chunyan
Y1  - 2021/05/24
PY  - 2021
N1  - https://doi.org/10.11648/j.ajcem.20210903.11
DO  - 10.11648/j.ajcem.20210903.11
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 44
EP  - 54
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20210903.11
AB  - Background: Research has shown that STAT gene SNP is related to the pathogenesis of T1D, SLE, RA and AITD. Objective: To investigate the correlation of the SNP of Th17/Treg cell and its cytokines with STAT4 in the PBMC of AITD patients with different iodine nutritious status. Method: 100 GD and HT patients who are selected from Cangzhou Central Hospital and Cangzhou People’s Hospital from September 2019 to August 2020, respectively, 60 healthy cases, adopt flow cytometer and quantitative real-time PCR to test the Th17and Treg cell ratio and proportion in PBMC and the expression level of ROR-γt and 3 (Foxp3) mRNA, and use ELISA method to test IL-17 and TGF-β level in the serum. Utilize SASP-PCR technology to test SNP locus (rs7574865) of STAT4 gene, and calculate the genotype and allele frequency. Result: There is some correlation between Th17/Treg cell & its factors in PBMC in the AITD patients and STAT4 rs7574865; compared with healthy control group, the difference between STAT4 rs7574865 G/T &T/T genotype and G&T allele frequency is of statistic significance (χ2 is 6.128 and 5.613 respectively, and the average value of P is <0.05), and may increase the occurrence risk of AITD, but has no correlation with iodine nutritious status. Conclusion: AITD patients not only have Th17 and Treg cell imbalance and the corresponding factor changes but also correlate to STAT4 rs7574865 susceptibility, and the cellular immunity and gene SNP may participate in the disease occurrence process of AIDT together.
VL  - 9
IS  - 3
ER  -

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Author Information

Feng Jing

Cangzhou Central Hospital, Cangzhou, China
Mu Zhaoxin

Institute of Thyroid Diseases Affiliated to Cangzhou Medical College, Cangzhou Thyroid Disease Engineering Technology Research Center, Cangzhou, China
Hou Zhenjiang

Institute of Thyroid Diseases Affiliated to Cangzhou Medical College, Cangzhou Thyroid Disease Engineering Technology Research Center, Cangzhou, China
Wang Cuicui

Institute of Thyroid Diseases Affiliated to Cangzhou Medical College, Cangzhou Thyroid Disease Engineering Technology Research Center, Cangzhou, China
Li Xinsheng

Cangzhou Central Hospital, Cangzhou, China
Chen Yunxia

Cangzhou People's Hospital Endocrinology, Cangzhou, China
Liu Jianfeng

Cangzhou People's Hospital Endocrinology, Cangzhou, China
Ma Jinqun

Cangzhou People's Hospital Endocrinology, Cangzhou, China
Liu Chunyan

Cangzhou People's Hospital Endocrinology, Cangzhou, China

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APA Style

Feng Jing, Mu Zhaoxin, Hou Zhenjiang, Wang Cuicui, Li Xinsheng, et al. (2021). Research on Polymorphism Relationship of Th17/Treg Cell and Its Factors with STAT4 Gene in AITD Patients with Different Iodine Nutritional Status. American Journal of Clinical and Experimental Medicine, 9(3), 44-54. https://doi.org/10.11648/j.ajcem.20210903.11

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ACS Style

Feng Jing; Mu Zhaoxin; Hou Zhenjiang; Wang Cuicui; Li Xinsheng, et al. Research on Polymorphism Relationship of Th17/Treg Cell and Its Factors with STAT4 Gene in AITD Patients with Different Iodine Nutritional Status. Am. J. Clin. Exp. Med. 2021, 9(3), 44-54. doi: 10.11648/j.ajcem.20210903.11

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AMA Style

Feng Jing, Mu Zhaoxin, Hou Zhenjiang, Wang Cuicui, Li Xinsheng, et al. Research on Polymorphism Relationship of Th17/Treg Cell and Its Factors with STAT4 Gene in AITD Patients with Different Iodine Nutritional Status. Am J Clin Exp Med. 2021;9(3):44-54. doi: 10.11648/j.ajcem.20210903.11

Copy | Download

@article{10.11648/j.ajcem.20210903.11,
  author = {Feng Jing and Mu Zhaoxin and Hou Zhenjiang and Wang Cuicui and Li Xinsheng and Chen Yunxia and Liu Jianfeng and Ma Jinqun and Liu Chunyan},
  title = {Research on Polymorphism Relationship of Th17/Treg Cell and Its Factors with STAT4 Gene in AITD Patients with Different Iodine Nutritional Status},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {9},
  number = {3},
  pages = {44-54},
  doi = {10.11648/j.ajcem.20210903.11},
  url = {https://doi.org/10.11648/j.ajcem.20210903.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20210903.11},
  abstract = {Background: Research has shown that STAT gene SNP is related to the pathogenesis of T1D, SLE, RA and AITD. Objective: To investigate the correlation of the SNP of Th17/Treg cell and its cytokines with STAT4 in the PBMC of AITD patients with different iodine nutritious status. Method: 100 GD and HT patients who are selected from Cangzhou Central Hospital and Cangzhou People’s Hospital from September 2019 to August 2020, respectively, 60 healthy cases, adopt flow cytometer and quantitative real-time PCR to test the Th17and Treg cell ratio and proportion in PBMC and the expression level of ROR-γt and 3 (Foxp3) mRNA, and use ELISA method to test IL-17 and TGF-β level in the serum. Utilize SASP-PCR technology to test SNP locus (rs7574865) of STAT4 gene, and calculate the genotype and allele frequency. Result: There is some correlation between Th17/Treg cell & its factors in PBMC in the AITD patients and STAT4 rs7574865; compared with healthy control group, the difference between STAT4 rs7574865 G/T &T/T genotype and G&T allele frequency is of statistic significance (χ2 is 6.128 and 5.613 respectively, and the average value of P is <0.05), and may increase the occurrence risk of AITD, but has no correlation with iodine nutritious status. Conclusion: AITD patients not only have Th17 and Treg cell imbalance and the corresponding factor changes but also correlate to STAT4 rs7574865 susceptibility, and the cellular immunity and gene SNP may participate in the disease occurrence process of AIDT together.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Research on Polymorphism Relationship of Th17/Treg Cell and Its Factors with STAT4 Gene in AITD Patients with Different Iodine Nutritional Status
AU  - Feng Jing
AU  - Mu Zhaoxin
AU  - Hou Zhenjiang
AU  - Wang Cuicui
AU  - Li Xinsheng
AU  - Chen Yunxia
AU  - Liu Jianfeng
AU  - Ma Jinqun
AU  - Liu Chunyan
Y1  - 2021/05/24
PY  - 2021
N1  - https://doi.org/10.11648/j.ajcem.20210903.11
DO  - 10.11648/j.ajcem.20210903.11
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 44
EP  - 54
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20210903.11
AB  - Background: Research has shown that STAT gene SNP is related to the pathogenesis of T1D, SLE, RA and AITD. Objective: To investigate the correlation of the SNP of Th17/Treg cell and its cytokines with STAT4 in the PBMC of AITD patients with different iodine nutritious status. Method: 100 GD and HT patients who are selected from Cangzhou Central Hospital and Cangzhou People’s Hospital from September 2019 to August 2020, respectively, 60 healthy cases, adopt flow cytometer and quantitative real-time PCR to test the Th17and Treg cell ratio and proportion in PBMC and the expression level of ROR-γt and 3 (Foxp3) mRNA, and use ELISA method to test IL-17 and TGF-β level in the serum. Utilize SASP-PCR technology to test SNP locus (rs7574865) of STAT4 gene, and calculate the genotype and allele frequency. Result: There is some correlation between Th17/Treg cell & its factors in PBMC in the AITD patients and STAT4 rs7574865; compared with healthy control group, the difference between STAT4 rs7574865 G/T &T/T genotype and G&T allele frequency is of statistic significance (χ2 is 6.128 and 5.613 respectively, and the average value of P is <0.05), and may increase the occurrence risk of AITD, but has no correlation with iodine nutritious status. Conclusion: AITD patients not only have Th17 and Treg cell imbalance and the corresponding factor changes but also correlate to STAT4 rs7574865 susceptibility, and the cellular immunity and gene SNP may participate in the disease occurrence process of AIDT together.
VL  - 9
IS  - 3
ER  -

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