Aim: The HFMD caused by EV71 virus has extremely high lethality and morbidity. EV71's pathogenesis has not understood thoroughly and the antiviral drug has been under development, which led to its emergence as a clinically fatal neurotropic virus. Early diagnosis is one of the main methods to reduce mortality. The aim of this study was to establish and evaluate a colloidal gold immunochromatography for qualitatively detecting the EV71-IgM in serum sample. Method: In this study, the different kinds of coating antigen were the focus of optimization. The pH value, particle size of the colloidal gold and other parameters and materials were also considered to achieve the good performance in detecting strips. The CA16-positive, EV71-positive and negative serum were used to compare and evaluate the accuracy and specificity of strips. Result: The accuracy of the strips coated by EV71-VP1-VP2-VP3 was higher than the EV71-VP1 strips 20%. The specificity of the EV71-VP1 strips was slightly higher than EV71-VP1-VP2-VP3 strips according to CA16 positive serum samples. Compared with commercial strips, this diagnostic method just needed 2 µL serum samples for detection, and the accuracy of the EV71-VP1-VP2-VP3 strips was significantly higher than the commercial strips. Conclusion: The developed method established in this study can apply to early diagnosis of HFMD caused by EV71 virus.
| Published in | American Journal of Clinical and Experimental Medicine (Volume 6, Issue 4) |
| DOI | 10.11648/j.ajcem.20180604.13 |
| Page(s) | 99-102 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
HFMD, EV71-IgM, Colloidal Gold
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APA Style
Junwei Liu, Jiaxing Wang, Jinfeng Wang, Li Liu, Jin Li, et al. (2018). Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM. American Journal of Clinical and Experimental Medicine, 6(4), 99-102. https://doi.org/10.11648/j.ajcem.20180604.13
ACS Style
Junwei Liu; Jiaxing Wang; Jinfeng Wang; Li Liu; Jin Li, et al. Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM. Am. J. Clin. Exp. Med. 2018, 6(4), 99-102. doi: 10.11648/j.ajcem.20180604.13
AMA Style
Junwei Liu, Jiaxing Wang, Jinfeng Wang, Li Liu, Jin Li, et al. Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM. Am J Clin Exp Med. 2018;6(4):99-102. doi: 10.11648/j.ajcem.20180604.13
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Download@article{10.11648/j.ajcem.20180604.13,
author = {Junwei Liu and Jiaxing Wang and Jinfeng Wang and Li Liu and Jin Li and Haiqiu Ma},
title = {Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM},
journal = {American Journal of Clinical and Experimental Medicine},
volume = {6},
number = {4},
pages = {99-102},
doi = {10.11648/j.ajcem.20180604.13},
url = {https://doi.org/10.11648/j.ajcem.20180604.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20180604.13},
abstract = {Aim: The HFMD caused by EV71 virus has extremely high lethality and morbidity. EV71's pathogenesis has not understood thoroughly and the antiviral drug has been under development, which led to its emergence as a clinically fatal neurotropic virus. Early diagnosis is one of the main methods to reduce mortality. The aim of this study was to establish and evaluate a colloidal gold immunochromatography for qualitatively detecting the EV71-IgM in serum sample. Method: In this study, the different kinds of coating antigen were the focus of optimization. The pH value, particle size of the colloidal gold and other parameters and materials were also considered to achieve the good performance in detecting strips. The CA16-positive, EV71-positive and negative serum were used to compare and evaluate the accuracy and specificity of strips. Result: The accuracy of the strips coated by EV71-VP1-VP2-VP3 was higher than the EV71-VP1 strips 20%. The specificity of the EV71-VP1 strips was slightly higher than EV71-VP1-VP2-VP3 strips according to CA16 positive serum samples. Compared with commercial strips, this diagnostic method just needed 2 µL serum samples for detection, and the accuracy of the EV71-VP1-VP2-VP3 strips was significantly higher than the commercial strips. Conclusion: The developed method established in this study can apply to early diagnosis of HFMD caused by EV71 virus.},
year = {2018}
}
TY - JOUR T1 - Establishment and Evaluation of Colloidal Gold Immunochromatography Assay for Detection of EV71-IgM AU - Junwei Liu AU - Jiaxing Wang AU - Jinfeng Wang AU - Li Liu AU - Jin Li AU - Haiqiu Ma Y1 - 2018/10/18 PY - 2018 N1 - https://doi.org/10.11648/j.ajcem.20180604.13 DO - 10.11648/j.ajcem.20180604.13 T2 - American Journal of Clinical and Experimental Medicine JF - American Journal of Clinical and Experimental Medicine JO - American Journal of Clinical and Experimental Medicine SP - 99 EP - 102 PB - Science Publishing Group SN - 2330-8133 UR - https://doi.org/10.11648/j.ajcem.20180604.13 AB - Aim: The HFMD caused by EV71 virus has extremely high lethality and morbidity. EV71's pathogenesis has not understood thoroughly and the antiviral drug has been under development, which led to its emergence as a clinically fatal neurotropic virus. Early diagnosis is one of the main methods to reduce mortality. The aim of this study was to establish and evaluate a colloidal gold immunochromatography for qualitatively detecting the EV71-IgM in serum sample. Method: In this study, the different kinds of coating antigen were the focus of optimization. The pH value, particle size of the colloidal gold and other parameters and materials were also considered to achieve the good performance in detecting strips. The CA16-positive, EV71-positive and negative serum were used to compare and evaluate the accuracy and specificity of strips. Result: The accuracy of the strips coated by EV71-VP1-VP2-VP3 was higher than the EV71-VP1 strips 20%. The specificity of the EV71-VP1 strips was slightly higher than EV71-VP1-VP2-VP3 strips according to CA16 positive serum samples. Compared with commercial strips, this diagnostic method just needed 2 µL serum samples for detection, and the accuracy of the EV71-VP1-VP2-VP3 strips was significantly higher than the commercial strips. Conclusion: The developed method established in this study can apply to early diagnosis of HFMD caused by EV71 virus. VL - 6 IS - 4 ER -
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