The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure

Ramona Utami; Anis Irawan Anwar; Khairuddin Djawad; Anni Adriani; Siswanto Wahab; Burhanuddin Bahar

doi:doi:10.11648/j.ajcem.20170502.14

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The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure

Ramona Utami, Anis Irawan Anwar, Khairuddin Djawad, Anni Adriani, Siswanto Wahab, Burhanuddin Bahar

Published in American Journal of Clinical and Experimental Medicine (Volume 5, Issue 2)

Received: 25 January 2017 Accepted: 10 February 2017 Published: 2 March 2017

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Abstract

Ultraviolet B radiation (UVB) often cause oxidative stress, through the reactive oxygen species (ROS) formation and produce DNA damage. This study aims to assess the protective effects of topically cocoa extract lotio against DNA damage by measure of 8-OHdG level in albino mice after UVB exposure. This study was conducted in Makassar, South Sulawesi, using 20 Swiss albino mice, aged 6-9 weeks, weight 20-30 grams. Mice were divided into 3 groups: The first group is a control group or negative control (without cocoa extract and without UVB exposure, consist 4 mice), the second is UVB control group exposure with UVB 450mJ / cm² three times a week for 12 weeks without topical cocoa extract (4 mice) and the third group is mice with extract cocoa 200, 400 and 800 ppm every day, 20 minutes before exposure with UVB 450mJ / cm2 three times a week for 12 weeks (each concentration of cocoa extracts consist of 4 mice). At 24 hours after last exposure all mice were terminated and excisional biopsy on the dorsal skin of the size of 1x1 cm and performed 8-OHdG ELISA. The results show there was significant difference (p <0.05) between the control group UVB with negative control and cocoa 800 ppm. The 800 ppm cocoa extract is the most effective to the reduction of 8-OhdG level.

Published in	American Journal of Clinical and Experimental Medicine (Volume 5, Issue 2)
DOI	10.11648/j.ajcem.20170502.14
Page(s)	46-49
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

8-OHdG, Cocoa Extract Lotio, UVB

References

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[18]	Adriani A, Armin SA, Massi MN, Djawad K. Protective effects of daily topical cocoa extract to the expression of 8-OhdG and PCNA on UVB-exposed albino mice. Int J Biol Med Res. 2014; 5 (4): 4607-11.
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APA Style

Ramona Utami, Anis Irawan Anwar, Khairuddin Djawad, Anni Adriani, Siswanto Wahab, et al. (2017). The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure. American Journal of Clinical and Experimental Medicine, 5(2), 46-49. https://doi.org/10.11648/j.ajcem.20170502.14

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ACS Style

Ramona Utami; Anis Irawan Anwar; Khairuddin Djawad; Anni Adriani; Siswanto Wahab, et al. The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure. Am. J. Clin. Exp. Med. 2017, 5(2), 46-49. doi: 10.11648/j.ajcem.20170502.14

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AMA Style

Ramona Utami, Anis Irawan Anwar, Khairuddin Djawad, Anni Adriani, Siswanto Wahab, et al. The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure. Am J Clin Exp Med. 2017;5(2):46-49. doi: 10.11648/j.ajcem.20170502.14

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@article{10.11648/j.ajcem.20170502.14,
  author = {Ramona Utami and Anis Irawan Anwar and Khairuddin Djawad and Anni Adriani and Siswanto Wahab and Burhanuddin Bahar},
  title = {The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {5},
  number = {2},
  pages = {46-49},
  doi = {10.11648/j.ajcem.20170502.14},
  url = {https://doi.org/10.11648/j.ajcem.20170502.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20170502.14},
  abstract = {Ultraviolet B radiation (UVB) often cause oxidative stress, through the reactive oxygen species (ROS) formation and produce DNA damage. This study aims to assess the protective effects of topically cocoa extract lotio against DNA damage by measure of 8-OHdG level in albino mice after UVB exposure. This study was conducted in Makassar, South Sulawesi, using 20 Swiss albino mice, aged 6-9 weeks, weight 20-30 grams. Mice were divided into 3 groups: The first group is a control group or negative control (without cocoa extract and without UVB exposure, consist 4 mice), the second is UVB control group exposure with UVB 450mJ / cm2 three times a week for 12 weeks without topical cocoa extract (4 mice) and the third group is mice with extract cocoa 200, 400 and 800 ppm every day, 20 minutes before exposure with UVB 450mJ / cm2 three times a week for 12 weeks (each concentration of cocoa extracts consist of 4 mice). At 24 hours after last exposure all mice were terminated and excisional biopsy on the dorsal skin of the size of 1x1 cm and performed 8-OHdG ELISA. The results show there was significant difference (p <0.05) between the control group UVB with negative control and cocoa 800 ppm. The 800 ppm cocoa extract is the most effective to the reduction of 8-OhdG level.},
 year = {2017}
}

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TY  - JOUR
T1  - The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure
AU  - Ramona Utami
AU  - Anis Irawan Anwar
AU  - Khairuddin Djawad
AU  - Anni Adriani
AU  - Siswanto Wahab
AU  - Burhanuddin Bahar
Y1  - 2017/03/02
PY  - 2017
N1  - https://doi.org/10.11648/j.ajcem.20170502.14
DO  - 10.11648/j.ajcem.20170502.14
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 46
EP  - 49
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20170502.14
AB  - Ultraviolet B radiation (UVB) often cause oxidative stress, through the reactive oxygen species (ROS) formation and produce DNA damage. This study aims to assess the protective effects of topically cocoa extract lotio against DNA damage by measure of 8-OHdG level in albino mice after UVB exposure. This study was conducted in Makassar, South Sulawesi, using 20 Swiss albino mice, aged 6-9 weeks, weight 20-30 grams. Mice were divided into 3 groups: The first group is a control group or negative control (without cocoa extract and without UVB exposure, consist 4 mice), the second is UVB control group exposure with UVB 450mJ / cm2 three times a week for 12 weeks without topical cocoa extract (4 mice) and the third group is mice with extract cocoa 200, 400 and 800 ppm every day, 20 minutes before exposure with UVB 450mJ / cm2 three times a week for 12 weeks (each concentration of cocoa extracts consist of 4 mice). At 24 hours after last exposure all mice were terminated and excisional biopsy on the dorsal skin of the size of 1x1 cm and performed 8-OHdG ELISA. The results show there was significant difference (p <0.05) between the control group UVB with negative control and cocoa 800 ppm. The 800 ppm cocoa extract is the most effective to the reduction of 8-OhdG level.
VL  - 5
IS  - 2
ER  -

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Author Information

Ramona Utami

Departement of Dermatology and Venereology, Medical Faculty, Hasanudin University, Makassar, Indonesia
Anis Irawan Anwar

Departement of Dermatology and Venereology, Medical Faculty, Hasanudin University, Makassar, Indonesia
Khairuddin Djawad

Departement of Dermatology and Venereology, Medical Faculty, Hasanudin University, Makassar, Indonesia
Anni Adriani

Departement of Dermatology and Venereology, Medical Faculty, Hasanudin University, Makassar, Indonesia
Siswanto Wahab

Departement of Dermatology and Venereology, Medical Faculty, Hasanudin University, Makassar, Indonesia
Burhanuddin Bahar

Departement of Biostatistic, Medical Faculty, Hasanuddin University, Makassar, Indonesia

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Plain Text BibTeX RIS

APA Style

Ramona Utami, Anis Irawan Anwar, Khairuddin Djawad, Anni Adriani, Siswanto Wahab, et al. (2017). The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure. American Journal of Clinical and Experimental Medicine, 5(2), 46-49. https://doi.org/10.11648/j.ajcem.20170502.14

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ACS Style

Ramona Utami; Anis Irawan Anwar; Khairuddin Djawad; Anni Adriani; Siswanto Wahab, et al. The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure. Am. J. Clin. Exp. Med. 2017, 5(2), 46-49. doi: 10.11648/j.ajcem.20170502.14

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AMA Style

Ramona Utami, Anis Irawan Anwar, Khairuddin Djawad, Anni Adriani, Siswanto Wahab, et al. The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure. Am J Clin Exp Med. 2017;5(2):46-49. doi: 10.11648/j.ajcem.20170502.14

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@article{10.11648/j.ajcem.20170502.14,
  author = {Ramona Utami and Anis Irawan Anwar and Khairuddin Djawad and Anni Adriani and Siswanto Wahab and Burhanuddin Bahar},
  title = {The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {5},
  number = {2},
  pages = {46-49},
  doi = {10.11648/j.ajcem.20170502.14},
  url = {https://doi.org/10.11648/j.ajcem.20170502.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20170502.14},
  abstract = {Ultraviolet B radiation (UVB) often cause oxidative stress, through the reactive oxygen species (ROS) formation and produce DNA damage. This study aims to assess the protective effects of topically cocoa extract lotio against DNA damage by measure of 8-OHdG level in albino mice after UVB exposure. This study was conducted in Makassar, South Sulawesi, using 20 Swiss albino mice, aged 6-9 weeks, weight 20-30 grams. Mice were divided into 3 groups: The first group is a control group or negative control (without cocoa extract and without UVB exposure, consist 4 mice), the second is UVB control group exposure with UVB 450mJ / cm2 three times a week for 12 weeks without topical cocoa extract (4 mice) and the third group is mice with extract cocoa 200, 400 and 800 ppm every day, 20 minutes before exposure with UVB 450mJ / cm2 three times a week for 12 weeks (each concentration of cocoa extracts consist of 4 mice). At 24 hours after last exposure all mice were terminated and excisional biopsy on the dorsal skin of the size of 1x1 cm and performed 8-OHdG ELISA. The results show there was significant difference (p <0.05) between the control group UVB with negative control and cocoa 800 ppm. The 800 ppm cocoa extract is the most effective to the reduction of 8-OhdG level.},
 year = {2017}
}

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TY  - JOUR
T1  - The Protective Effects of Topical Cocoa Extract Lotio on Albino Mice to the Level of 8-OHdG After Ultraviolet B Exposure
AU  - Ramona Utami
AU  - Anis Irawan Anwar
AU  - Khairuddin Djawad
AU  - Anni Adriani
AU  - Siswanto Wahab
AU  - Burhanuddin Bahar
Y1  - 2017/03/02
PY  - 2017
N1  - https://doi.org/10.11648/j.ajcem.20170502.14
DO  - 10.11648/j.ajcem.20170502.14
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 46
EP  - 49
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20170502.14
AB  - Ultraviolet B radiation (UVB) often cause oxidative stress, through the reactive oxygen species (ROS) formation and produce DNA damage. This study aims to assess the protective effects of topically cocoa extract lotio against DNA damage by measure of 8-OHdG level in albino mice after UVB exposure. This study was conducted in Makassar, South Sulawesi, using 20 Swiss albino mice, aged 6-9 weeks, weight 20-30 grams. Mice were divided into 3 groups: The first group is a control group or negative control (without cocoa extract and without UVB exposure, consist 4 mice), the second is UVB control group exposure with UVB 450mJ / cm2 three times a week for 12 weeks without topical cocoa extract (4 mice) and the third group is mice with extract cocoa 200, 400 and 800 ppm every day, 20 minutes before exposure with UVB 450mJ / cm2 three times a week for 12 weeks (each concentration of cocoa extracts consist of 4 mice). At 24 hours after last exposure all mice were terminated and excisional biopsy on the dorsal skin of the size of 1x1 cm and performed 8-OHdG ELISA. The results show there was significant difference (p <0.05) between the control group UVB with negative control and cocoa 800 ppm. The 800 ppm cocoa extract is the most effective to the reduction of 8-OhdG level.
VL  - 5
IS  - 2
ER  -

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