Protective Effect of Cocoa Extract on Malondialdehyde Level in Ultraviolet B Induced - Albino Mice Skin

Suci Nugraeni Tahir; Farida Tabri; Khairuddin Djawad; Anni Adriani; Arifin Seweng; Nasrum Massi

doi:doi:10.11648/j.ajcem.20170502.13

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Protective Effect of Cocoa Extract on Malondialdehyde Level in Ultraviolet B Induced - Albino Mice Skin

Suci Nugraeni Tahir, Farida Tabri, Khairuddin Djawad, Anni Adriani, Arifin Seweng, Nasrum Massi

Published in American Journal of Clinical and Experimental Medicine (Volume 5, Issue 2)

Received: 27 January 2017 Accepted: 10 February 2017 Published: 1 March 2017

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Abstract

The antioxidant activity in cocoa linked to the polyphenol content therein, especially subunit monomers catechin and epicatechin. The aim of the study was to determine the protective effects of cocoa extract on levels of malondialdehyde in albino mice by exposure to UVB. This study was conducted at animal laboratory to interventions UVB and Extract, Cocoa and Biomolecular laboratory of medical faculty of Hasanuddin University for the ELISA examination. This study used true experimental design with animal experimental design to assess the effectiveness of topical application cacao extract on mice skin after UVB induction for twelve weeks. The research samples were 30 head of albino mice divided into 6 groups: control 5 mice without protection and given 450 mJ UV Bexposure 3 times per week, the second group was 5 untreated controlmice, the third group was 5 mice with 100 ppm of cocoa topical extract every day and UVB 450 mJ exposure 3 times a week, the fourth group of 5 mice with 200 ppm topical cocoa extract every day and exposure to 450 mJ three times a week, the fifth group of 5 mice with 400 ppm topical cocoa extract every day and exposure to 450 mJ three times a week, the sixth group of 5 mice with 800 ppm topical everyday cocoa extract and exposure to 450 mJ three times a week, a week after termination and biopsy excisionof skin for examination of Malondialdehyde level with ELISA. The results indicated resources of 200 ppm cocoa extract Provides the best effect with reduced MDA levels with the highest protective effect Compared to other groups.

Published in	American Journal of Clinical and Experimental Medicine (Volume 5, Issue 2)
DOI	10.11648/j.ajcem.20170502.13
Page(s)	41-45
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Cocoa Extract, Malondialdehyde, Ultraviolet B

References

[1]	Morganelli. (2006). Biography of Chocolate (Paperback). Canada: Crabtree Publishing Co.
[2]	Gasser et al. (2008). Cocoa polyphenols and Their Influence on Parameters Involved in ex Vivo Skin Restructuring. Int J Cosmet Sci. 30 (5): 339-45.
[3]	McShea et al. (2008). Clinical Benefit and Preservation of flavonols in Dark Chocolate Manufacturing. Nutrition reviews. 66 (11): 630-641.
[4]	Othman et al. (2010). Epicatechin Content and Antioxidant Capacity of Cocoa Beans from Four Different Countries. African Journal of Biotechnology. 9 (7): 1052-1059.
[5]	Valko et al. (2007). Free Radicals and Antioxidants in Normal Physiological Functions and Human Disease. Int J Biochem Cell Biol. 39 (1): 44-48.
[6]	Godic et al. (2014). The Role of Antioxidants in Skin Cancer Prevention and Treatment. Oxidative Medicine and Cellular Longevity.
[7]	Zajac et al. (2008). Food Webs in Long Island Sound: Review, Synthesis and Potential Applications. United States Environmental Protection Agency.
[8]	Djawad et al. (2008). Photoprotective effect Curcumin Against Expression CPD, 8-OHdG, Apoptosis and hyperplasia of the epidermis. Makassar: Hasanuddin University.
[9]	Waspodo et al. (2012). Role of Cocoa Bean Extract Against Aging Skin After Exposure to Ultraviolet B (Expression of transforming growth factor β and matrix Metalloproitenase I. Makassar: Hasanuddin University.
[10]	Adriani et al. (2014). Protective Effects of Daily Topical Cocoa Extract to the Expression of 8 OHdG and PCNA on UVB-Exposed Albino Mice. Int J Med Biol Res. 5 (4): 4607-11.
[11]	Martin et al. (2008). Protection of Human HepG2 Cells Against Oxidative Stress by CocoaPhenolicExtract. J. Agric. Food Chem. 56: 7765-7772.
[12]	Bouayed & Bohn. (2010). Exogenous Antioxidants-Double-edged Swords in Cellular Redox State: Beneficial Health Effects at physiologic doses Versus deleterious Effects at high doses. Oxid Med Cell Longev. 3 (4): 228-37.
[13]	Sies & Jones (2007). Oxidative Stress. In: Fink G, editors. Encyclopedia of Stress. San Diego: Elsevier.

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Suci Nugraeni Tahir, Farida Tabri, Khairuddin Djawad, Anni Adriani, Arifin Seweng, et al. (2017). Protective Effect of Cocoa Extract on Malondialdehyde Level in Ultraviolet B Induced - Albino Mice Skin. American Journal of Clinical and Experimental Medicine, 5(2), 41-45. https://doi.org/10.11648/j.ajcem.20170502.13

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ACS Style

Suci Nugraeni Tahir; Farida Tabri; Khairuddin Djawad; Anni Adriani; Arifin Seweng, et al. Protective Effect of Cocoa Extract on Malondialdehyde Level in Ultraviolet B Induced - Albino Mice Skin. Am. J. Clin. Exp. Med. 2017, 5(2), 41-45. doi: 10.11648/j.ajcem.20170502.13

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AMA Style

Suci Nugraeni Tahir, Farida Tabri, Khairuddin Djawad, Anni Adriani, Arifin Seweng, et al. Protective Effect of Cocoa Extract on Malondialdehyde Level in Ultraviolet B Induced - Albino Mice Skin. Am J Clin Exp Med. 2017;5(2):41-45. doi: 10.11648/j.ajcem.20170502.13

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@article{10.11648/j.ajcem.20170502.13,
  author = {Suci Nugraeni Tahir and Farida Tabri and Khairuddin Djawad and Anni Adriani and Arifin Seweng and Nasrum Massi},
  title = {Protective Effect of Cocoa Extract on Malondialdehyde Level in Ultraviolet B Induced - Albino Mice Skin},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {5},
  number = {2},
  pages = {41-45},
  doi = {10.11648/j.ajcem.20170502.13},
  url = {https://doi.org/10.11648/j.ajcem.20170502.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20170502.13},
  abstract = {The antioxidant activity in cocoa linked to the polyphenol content therein, especially subunit monomers catechin and epicatechin. The aim of the study was to determine the protective effects of cocoa extract on levels of malondialdehyde in albino mice by exposure to UVB. This study was conducted at animal laboratory to interventions UVB and Extract, Cocoa and Biomolecular laboratory of medical faculty of Hasanuddin University for the ELISA examination. This study used true experimental design with animal experimental design to assess the effectiveness of topical application cacao extract on mice skin after UVB induction for twelve weeks. The research samples were 30 head of albino mice divided into 6 groups: control 5 mice without protection and given 450 mJ UV Bexposure 3 times per week, the second group was 5 untreated controlmice, the third group was 5 mice with 100 ppm of cocoa topical extract every day and UVB 450 mJ exposure 3 times a week, the fourth group of 5 mice with 200 ppm topical cocoa extract every day and exposure to 450 mJ three times a week, the fifth group of 5 mice with 400 ppm topical cocoa extract every day and exposure to 450 mJ three times a week, the sixth group of 5 mice with 800 ppm topical everyday cocoa extract and exposure to 450 mJ three times a week, a week after termination and biopsy excisionof skin for examination of Malondialdehyde level with ELISA. The results indicated resources of 200 ppm cocoa extract Provides the best effect with reduced MDA levels with the highest protective effect Compared to other groups.},
 year = {2017}
}

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TY  - JOUR
T1  - Protective Effect of Cocoa Extract on Malondialdehyde Level in Ultraviolet B Induced - Albino Mice Skin
AU  - Suci Nugraeni Tahir
AU  - Farida Tabri
AU  - Khairuddin Djawad
AU  - Anni Adriani
AU  - Arifin Seweng
AU  - Nasrum Massi
Y1  - 2017/03/01
PY  - 2017
N1  - https://doi.org/10.11648/j.ajcem.20170502.13
DO  - 10.11648/j.ajcem.20170502.13
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 41
EP  - 45
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20170502.13
AB  - The antioxidant activity in cocoa linked to the polyphenol content therein, especially subunit monomers catechin and epicatechin. The aim of the study was to determine the protective effects of cocoa extract on levels of malondialdehyde in albino mice by exposure to UVB. This study was conducted at animal laboratory to interventions UVB and Extract, Cocoa and Biomolecular laboratory of medical faculty of Hasanuddin University for the ELISA examination. This study used true experimental design with animal experimental design to assess the effectiveness of topical application cacao extract on mice skin after UVB induction for twelve weeks. The research samples were 30 head of albino mice divided into 6 groups: control 5 mice without protection and given 450 mJ UV Bexposure 3 times per week, the second group was 5 untreated controlmice, the third group was 5 mice with 100 ppm of cocoa topical extract every day and UVB 450 mJ exposure 3 times a week, the fourth group of 5 mice with 200 ppm topical cocoa extract every day and exposure to 450 mJ three times a week, the fifth group of 5 mice with 400 ppm topical cocoa extract every day and exposure to 450 mJ three times a week, the sixth group of 5 mice with 800 ppm topical everyday cocoa extract and exposure to 450 mJ three times a week, a week after termination and biopsy excisionof skin for examination of Malondialdehyde level with ELISA. The results indicated resources of 200 ppm cocoa extract Provides the best effect with reduced MDA levels with the highest protective effect Compared to other groups.
VL  - 5
IS  - 2
ER  -

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Author Information

Suci Nugraeni Tahir

Departement Dermatology and Venereal, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia
Farida Tabri

Departement Dermatology and Venereal, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia
Khairuddin Djawad

Departement Dermatology and Venereal, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia
Anni Adriani

Departement Dermatology and Venereal, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia
Arifin Seweng

Departement of Biostatistic, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia
Nasrum Massi

Departement of Microbiology, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia

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Plain Text BibTeX RIS

APA Style

Suci Nugraeni Tahir, Farida Tabri, Khairuddin Djawad, Anni Adriani, Arifin Seweng, et al. (2017). Protective Effect of Cocoa Extract on Malondialdehyde Level in Ultraviolet B Induced - Albino Mice Skin. American Journal of Clinical and Experimental Medicine, 5(2), 41-45. https://doi.org/10.11648/j.ajcem.20170502.13

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ACS Style

Suci Nugraeni Tahir; Farida Tabri; Khairuddin Djawad; Anni Adriani; Arifin Seweng, et al. Protective Effect of Cocoa Extract on Malondialdehyde Level in Ultraviolet B Induced - Albino Mice Skin. Am. J. Clin. Exp. Med. 2017, 5(2), 41-45. doi: 10.11648/j.ajcem.20170502.13

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AMA Style

Suci Nugraeni Tahir, Farida Tabri, Khairuddin Djawad, Anni Adriani, Arifin Seweng, et al. Protective Effect of Cocoa Extract on Malondialdehyde Level in Ultraviolet B Induced - Albino Mice Skin. Am J Clin Exp Med. 2017;5(2):41-45. doi: 10.11648/j.ajcem.20170502.13

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@article{10.11648/j.ajcem.20170502.13,
  author = {Suci Nugraeni Tahir and Farida Tabri and Khairuddin Djawad and Anni Adriani and Arifin Seweng and Nasrum Massi},
  title = {Protective Effect of Cocoa Extract on Malondialdehyde Level in Ultraviolet B Induced - Albino Mice Skin},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {5},
  number = {2},
  pages = {41-45},
  doi = {10.11648/j.ajcem.20170502.13},
  url = {https://doi.org/10.11648/j.ajcem.20170502.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20170502.13},
  abstract = {The antioxidant activity in cocoa linked to the polyphenol content therein, especially subunit monomers catechin and epicatechin. The aim of the study was to determine the protective effects of cocoa extract on levels of malondialdehyde in albino mice by exposure to UVB. This study was conducted at animal laboratory to interventions UVB and Extract, Cocoa and Biomolecular laboratory of medical faculty of Hasanuddin University for the ELISA examination. This study used true experimental design with animal experimental design to assess the effectiveness of topical application cacao extract on mice skin after UVB induction for twelve weeks. The research samples were 30 head of albino mice divided into 6 groups: control 5 mice without protection and given 450 mJ UV Bexposure 3 times per week, the second group was 5 untreated controlmice, the third group was 5 mice with 100 ppm of cocoa topical extract every day and UVB 450 mJ exposure 3 times a week, the fourth group of 5 mice with 200 ppm topical cocoa extract every day and exposure to 450 mJ three times a week, the fifth group of 5 mice with 400 ppm topical cocoa extract every day and exposure to 450 mJ three times a week, the sixth group of 5 mice with 800 ppm topical everyday cocoa extract and exposure to 450 mJ three times a week, a week after termination and biopsy excisionof skin for examination of Malondialdehyde level with ELISA. The results indicated resources of 200 ppm cocoa extract Provides the best effect with reduced MDA levels with the highest protective effect Compared to other groups.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Protective Effect of Cocoa Extract on Malondialdehyde Level in Ultraviolet B Induced - Albino Mice Skin
AU  - Suci Nugraeni Tahir
AU  - Farida Tabri
AU  - Khairuddin Djawad
AU  - Anni Adriani
AU  - Arifin Seweng
AU  - Nasrum Massi
Y1  - 2017/03/01
PY  - 2017
N1  - https://doi.org/10.11648/j.ajcem.20170502.13
DO  - 10.11648/j.ajcem.20170502.13
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 41
EP  - 45
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20170502.13
AB  - The antioxidant activity in cocoa linked to the polyphenol content therein, especially subunit monomers catechin and epicatechin. The aim of the study was to determine the protective effects of cocoa extract on levels of malondialdehyde in albino mice by exposure to UVB. This study was conducted at animal laboratory to interventions UVB and Extract, Cocoa and Biomolecular laboratory of medical faculty of Hasanuddin University for the ELISA examination. This study used true experimental design with animal experimental design to assess the effectiveness of topical application cacao extract on mice skin after UVB induction for twelve weeks. The research samples were 30 head of albino mice divided into 6 groups: control 5 mice without protection and given 450 mJ UV Bexposure 3 times per week, the second group was 5 untreated controlmice, the third group was 5 mice with 100 ppm of cocoa topical extract every day and UVB 450 mJ exposure 3 times a week, the fourth group of 5 mice with 200 ppm topical cocoa extract every day and exposure to 450 mJ three times a week, the fifth group of 5 mice with 400 ppm topical cocoa extract every day and exposure to 450 mJ three times a week, the sixth group of 5 mice with 800 ppm topical everyday cocoa extract and exposure to 450 mJ three times a week, a week after termination and biopsy excisionof skin for examination of Malondialdehyde level with ELISA. The results indicated resources of 200 ppm cocoa extract Provides the best effect with reduced MDA levels with the highest protective effect Compared to other groups.
VL  - 5
IS  - 2
ER  -

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