Insulin resistance is a major characteristic of type 2 diabetes (T2DM). Inflammation plays an important role in increased insulin resistance, but the underlying mechanism remains unclear. Interleukin-10 (IL-10) is an anti-inflammatory cytokine with lower circulating levels in T2DM. We aimed to examine the association between IL-10 and insulin resistance, and to evaluate IL-10 gene promoter single nucleotide polymorphism (SNP) at position -592 C/A and serum IL-10 level as risk factors for insulin resistance and T2DM. This study was carried out on 200 subjects divided into 2 groups: 110 patients with T2DM (group I), and 90 healthy subjects served as controls (group II). All participants were investigated for; fasting and 2 hour post prandial blood glucose, serum lipids, glycated hemoglobin (HbA1c), serum IL-10 and fasting serum insulin. HOMA-IR was used for assessment of insulin resistance and β cell activity. Genotyping of -592 C/A (rs1800872) SNP of IL-10 gene promoter and genotype frequencies were analyzed using the polymerase chain reaction–restriction fragment length polymorphism technique (PCR-RFLP). The results of the present study showed significant statistical decrease in serum IL-10 levels in group I compared to group II. A significant negative correlation was found between serum IL-10 and HOMA-IR. Significant differences were observed for -592 C/A genotype distributions between both groups with increased frequency of the AA genotype in diabetic patients and increased frequency of CC genotype in controls. AA genotypes of -592 C/A was found to be a genetic risk factor for T2DM. Our results show that IL-10 has a positive association with insulin sensitivity, and SNP-592 C/A of IL-10 gene promoter and its serum level can contribute to susceptibility to insulin resistance and T2DM.
| Published in | American Journal of Clinical and Experimental Medicine (Volume 4, Issue 3) |
| DOI | 10.11648/j.ajcem.20160403.18 |
| Page(s) | 81-87 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Type 2 Diabetes Mellitus, IL-10, Gene Polymorphism
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APA Style
Sally Said Donia, Eman Masoud Abd El Gayed, Sally Mohamed El-Hefnawy, Ahmed Ragheb. (2016). The Association Between Interleukin-10 Gene Promoter Polymorphism and Insulin Resistance in Type 2 Diabetes Mellitus. American Journal of Clinical and Experimental Medicine, 4(3), 81-87. https://doi.org/10.11648/j.ajcem.20160403.18
ACS Style
Sally Said Donia; Eman Masoud Abd El Gayed; Sally Mohamed El-Hefnawy; Ahmed Ragheb. The Association Between Interleukin-10 Gene Promoter Polymorphism and Insulin Resistance in Type 2 Diabetes Mellitus. Am. J. Clin. Exp. Med. 2016, 4(3), 81-87. doi: 10.11648/j.ajcem.20160403.18
AMA Style
Sally Said Donia, Eman Masoud Abd El Gayed, Sally Mohamed El-Hefnawy, Ahmed Ragheb. The Association Between Interleukin-10 Gene Promoter Polymorphism and Insulin Resistance in Type 2 Diabetes Mellitus. Am J Clin Exp Med. 2016;4(3):81-87. doi: 10.11648/j.ajcem.20160403.18
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Download@article{10.11648/j.ajcem.20160403.18,
author = {Sally Said Donia and Eman Masoud Abd El Gayed and Sally Mohamed El-Hefnawy and Ahmed Ragheb},
title = {The Association Between Interleukin-10 Gene Promoter Polymorphism and Insulin Resistance in Type 2 Diabetes Mellitus},
journal = {American Journal of Clinical and Experimental Medicine},
volume = {4},
number = {3},
pages = {81-87},
doi = {10.11648/j.ajcem.20160403.18},
url = {https://doi.org/10.11648/j.ajcem.20160403.18},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20160403.18},
abstract = {Insulin resistance is a major characteristic of type 2 diabetes (T2DM). Inflammation plays an important role in increased insulin resistance, but the underlying mechanism remains unclear. Interleukin-10 (IL-10) is an anti-inflammatory cytokine with lower circulating levels in T2DM. We aimed to examine the association between IL-10 and insulin resistance, and to evaluate IL-10 gene promoter single nucleotide polymorphism (SNP) at position -592 C/A and serum IL-10 level as risk factors for insulin resistance and T2DM. This study was carried out on 200 subjects divided into 2 groups: 110 patients with T2DM (group I), and 90 healthy subjects served as controls (group II). All participants were investigated for; fasting and 2 hour post prandial blood glucose, serum lipids, glycated hemoglobin (HbA1c), serum IL-10 and fasting serum insulin. HOMA-IR was used for assessment of insulin resistance and β cell activity. Genotyping of -592 C/A (rs1800872) SNP of IL-10 gene promoter and genotype frequencies were analyzed using the polymerase chain reaction–restriction fragment length polymorphism technique (PCR-RFLP). The results of the present study showed significant statistical decrease in serum IL-10 levels in group I compared to group II. A significant negative correlation was found between serum IL-10 and HOMA-IR. Significant differences were observed for -592 C/A genotype distributions between both groups with increased frequency of the AA genotype in diabetic patients and increased frequency of CC genotype in controls. AA genotypes of -592 C/A was found to be a genetic risk factor for T2DM. Our results show that IL-10 has a positive association with insulin sensitivity, and SNP-592 C/A of IL-10 gene promoter and its serum level can contribute to susceptibility to insulin resistance and T2DM.},
year = {2016}
}
TY - JOUR T1 - The Association Between Interleukin-10 Gene Promoter Polymorphism and Insulin Resistance in Type 2 Diabetes Mellitus AU - Sally Said Donia AU - Eman Masoud Abd El Gayed AU - Sally Mohamed El-Hefnawy AU - Ahmed Ragheb Y1 - 2016/05/28 PY - 2016 N1 - https://doi.org/10.11648/j.ajcem.20160403.18 DO - 10.11648/j.ajcem.20160403.18 T2 - American Journal of Clinical and Experimental Medicine JF - American Journal of Clinical and Experimental Medicine JO - American Journal of Clinical and Experimental Medicine SP - 81 EP - 87 PB - Science Publishing Group SN - 2330-8133 UR - https://doi.org/10.11648/j.ajcem.20160403.18 AB - Insulin resistance is a major characteristic of type 2 diabetes (T2DM). Inflammation plays an important role in increased insulin resistance, but the underlying mechanism remains unclear. Interleukin-10 (IL-10) is an anti-inflammatory cytokine with lower circulating levels in T2DM. We aimed to examine the association between IL-10 and insulin resistance, and to evaluate IL-10 gene promoter single nucleotide polymorphism (SNP) at position -592 C/A and serum IL-10 level as risk factors for insulin resistance and T2DM. This study was carried out on 200 subjects divided into 2 groups: 110 patients with T2DM (group I), and 90 healthy subjects served as controls (group II). All participants were investigated for; fasting and 2 hour post prandial blood glucose, serum lipids, glycated hemoglobin (HbA1c), serum IL-10 and fasting serum insulin. HOMA-IR was used for assessment of insulin resistance and β cell activity. Genotyping of -592 C/A (rs1800872) SNP of IL-10 gene promoter and genotype frequencies were analyzed using the polymerase chain reaction–restriction fragment length polymorphism technique (PCR-RFLP). The results of the present study showed significant statistical decrease in serum IL-10 levels in group I compared to group II. A significant negative correlation was found between serum IL-10 and HOMA-IR. Significant differences were observed for -592 C/A genotype distributions between both groups with increased frequency of the AA genotype in diabetic patients and increased frequency of CC genotype in controls. AA genotypes of -592 C/A was found to be a genetic risk factor for T2DM. Our results show that IL-10 has a positive association with insulin sensitivity, and SNP-592 C/A of IL-10 gene promoter and its serum level can contribute to susceptibility to insulin resistance and T2DM. VL - 4 IS - 3 ER -
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