Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss

Fadel A. Sharif; Mohammed J. Ashour; Naim T. Badawi; Shadi F. Al-Ashi

doi:doi:10.11648/j.ajcem.20160403.15

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Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss

Fadel A. Sharif, Mohammed J. Ashour, Naim T. Badawi, Shadi F. Al-Ashi

Published in American Journal of Clinical and Experimental Medicine (Volume 4, Issue 3)

Received: 21 April 2016 Accepted: 29 April 2016 Published: 11 May 2016

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Abstract

In the absence of confirmed causes for around 50% of recurrent pregnancy loss (RPL) cases this study was conducted in order to investigate the association between single nucleotide polymorphism (SNP) in regulatory T-cell related STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF (rs3753082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) genes and unexplained RPL in a group of Palestinian women residing in Gaza strip. A retrospective case-control study was carried out during the period (August 2015 to March 2016). A total of 200 females, 100 RPL patients and 100 control women without previous history of RPL, aged 20–35 years were included in the study. STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF (rs375082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) polymorphisms were tested by PCR-RFLP. Statistically significant difference existed between RPL cases and controls in terms of the genotypic distribution of the tested polymorphisms. STAT3 CC, FOXP3 AA, LIF CC, NKG7 AA and CCR5 GG genotypes were significantly higher in the RPL group. The tested polymorphisms shape the first elements of immune tolerance-related risk SNPs panel for RPL in the investigated population and may lead to improved therapeutic approaches.

Published in	American Journal of Clinical and Experimental Medicine (Volume 4, Issue 3)
DOI	10.11648/j.ajcem.20160403.15
Page(s)	63-67
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Regulatory T-cells, STAT3, FOXP3, LIF, NKG7, CCR5, Polymorphism, Recurrent Pregnancy Loss

References

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APA Style

Fadel A. Sharif, Mohammed J. Ashour, Naim T. Badawi, Shadi F. Al-Ashi. (2016). Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss. American Journal of Clinical and Experimental Medicine, 4(3), 63-67. https://doi.org/10.11648/j.ajcem.20160403.15

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ACS Style

Fadel A. Sharif; Mohammed J. Ashour; Naim T. Badawi; Shadi F. Al-Ashi. Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss. Am. J. Clin. Exp. Med. 2016, 4(3), 63-67. doi: 10.11648/j.ajcem.20160403.15

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AMA Style

Fadel A. Sharif, Mohammed J. Ashour, Naim T. Badawi, Shadi F. Al-Ashi. Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss. Am J Clin Exp Med. 2016;4(3):63-67. doi: 10.11648/j.ajcem.20160403.15

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@article{10.11648/j.ajcem.20160403.15,
  author = {Fadel A. Sharif and Mohammed J. Ashour and Naim T. Badawi and Shadi F. Al-Ashi},
  title = {Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {4},
  number = {3},
  pages = {63-67},
  doi = {10.11648/j.ajcem.20160403.15},
  url = {https://doi.org/10.11648/j.ajcem.20160403.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20160403.15},
  abstract = {In the absence of confirmed causes for around 50% of recurrent pregnancy loss (RPL) cases this study was conducted in order to investigate the association between single nucleotide polymorphism (SNP) in regulatory T-cell related STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF  (rs3753082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) genes and unexplained RPL in a group of Palestinian women residing in Gaza strip. A retrospective case-control study was carried out during the period (August 2015 to March 2016). A total of 200 females, 100 RPL patients and 100 control women without previous history of RPL, aged 20–35 years were included in the study. STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF  (rs375082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) polymorphisms were tested by PCR-RFLP. Statistically significant difference existed between RPL cases and controls in terms of the genotypic distribution of the tested polymorphisms. STAT3 CC, FOXP3 AA, LIF  CC, NKG7 AA and CCR5 GG genotypes were significantly higher in the RPL group. The tested polymorphisms shape the first elements of immune tolerance-related risk SNPs panel for RPL in the investigated population and may lead to improved therapeutic approaches.},
 year = {2016}
}

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TY  - JOUR
T1  - Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss
AU  - Fadel A. Sharif
AU  - Mohammed J. Ashour
AU  - Naim T. Badawi
AU  - Shadi F. Al-Ashi
Y1  - 2016/05/11
PY  - 2016
N1  - https://doi.org/10.11648/j.ajcem.20160403.15
DO  - 10.11648/j.ajcem.20160403.15
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 63
EP  - 67
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20160403.15
AB  - In the absence of confirmed causes for around 50% of recurrent pregnancy loss (RPL) cases this study was conducted in order to investigate the association between single nucleotide polymorphism (SNP) in regulatory T-cell related STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF  (rs3753082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) genes and unexplained RPL in a group of Palestinian women residing in Gaza strip. A retrospective case-control study was carried out during the period (August 2015 to March 2016). A total of 200 females, 100 RPL patients and 100 control women without previous history of RPL, aged 20–35 years were included in the study. STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF  (rs375082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) polymorphisms were tested by PCR-RFLP. Statistically significant difference existed between RPL cases and controls in terms of the genotypic distribution of the tested polymorphisms. STAT3 CC, FOXP3 AA, LIF  CC, NKG7 AA and CCR5 GG genotypes were significantly higher in the RPL group. The tested polymorphisms shape the first elements of immune tolerance-related risk SNPs panel for RPL in the investigated population and may lead to improved therapeutic approaches.
VL  - 4
IS  - 3
ER  -

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Author Information

Fadel A. Sharif

Department of Medical Laboratory Sciences, Islamic University of Gaza, Gaza, Palestine
Mohammed J. Ashour

Department of Medical Laboratory Sciences, Islamic University of Gaza, Gaza, Palestine
Naim T. Badawi

Department of Medical Laboratory Sciences, Islamic University of Gaza, Gaza, Palestine
Shadi F. Al-Ashi

Department of Medical Laboratory Sciences, Islamic University of Gaza, Gaza, Palestine

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APA Style

Fadel A. Sharif, Mohammed J. Ashour, Naim T. Badawi, Shadi F. Al-Ashi. (2016). Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss. American Journal of Clinical and Experimental Medicine, 4(3), 63-67. https://doi.org/10.11648/j.ajcem.20160403.15

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ACS Style

Fadel A. Sharif; Mohammed J. Ashour; Naim T. Badawi; Shadi F. Al-Ashi. Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss. Am. J. Clin. Exp. Med. 2016, 4(3), 63-67. doi: 10.11648/j.ajcem.20160403.15

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AMA Style

Fadel A. Sharif, Mohammed J. Ashour, Naim T. Badawi, Shadi F. Al-Ashi. Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss. Am J Clin Exp Med. 2016;4(3):63-67. doi: 10.11648/j.ajcem.20160403.15

Copy | Download

@article{10.11648/j.ajcem.20160403.15,
  author = {Fadel A. Sharif and Mohammed J. Ashour and Naim T. Badawi and Shadi F. Al-Ashi},
  title = {Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {4},
  number = {3},
  pages = {63-67},
  doi = {10.11648/j.ajcem.20160403.15},
  url = {https://doi.org/10.11648/j.ajcem.20160403.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20160403.15},
  abstract = {In the absence of confirmed causes for around 50% of recurrent pregnancy loss (RPL) cases this study was conducted in order to investigate the association between single nucleotide polymorphism (SNP) in regulatory T-cell related STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF  (rs3753082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) genes and unexplained RPL in a group of Palestinian women residing in Gaza strip. A retrospective case-control study was carried out during the period (August 2015 to March 2016). A total of 200 females, 100 RPL patients and 100 control women without previous history of RPL, aged 20–35 years were included in the study. STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF  (rs375082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) polymorphisms were tested by PCR-RFLP. Statistically significant difference existed between RPL cases and controls in terms of the genotypic distribution of the tested polymorphisms. STAT3 CC, FOXP3 AA, LIF  CC, NKG7 AA and CCR5 GG genotypes were significantly higher in the RPL group. The tested polymorphisms shape the first elements of immune tolerance-related risk SNPs panel for RPL in the investigated population and may lead to improved therapeutic approaches.},
 year = {2016}
}

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TY  - JOUR
T1  - Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss
AU  - Fadel A. Sharif
AU  - Mohammed J. Ashour
AU  - Naim T. Badawi
AU  - Shadi F. Al-Ashi
Y1  - 2016/05/11
PY  - 2016
N1  - https://doi.org/10.11648/j.ajcem.20160403.15
DO  - 10.11648/j.ajcem.20160403.15
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 63
EP  - 67
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20160403.15
AB  - In the absence of confirmed causes for around 50% of recurrent pregnancy loss (RPL) cases this study was conducted in order to investigate the association between single nucleotide polymorphism (SNP) in regulatory T-cell related STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF  (rs3753082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) genes and unexplained RPL in a group of Palestinian women residing in Gaza strip. A retrospective case-control study was carried out during the period (August 2015 to March 2016). A total of 200 females, 100 RPL patients and 100 control women without previous history of RPL, aged 20–35 years were included in the study. STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF  (rs375082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) polymorphisms were tested by PCR-RFLP. Statistically significant difference existed between RPL cases and controls in terms of the genotypic distribution of the tested polymorphisms. STAT3 CC, FOXP3 AA, LIF  CC, NKG7 AA and CCR5 GG genotypes were significantly higher in the RPL group. The tested polymorphisms shape the first elements of immune tolerance-related risk SNPs panel for RPL in the investigated population and may lead to improved therapeutic approaches.
VL  - 4
IS  - 3
ER  -

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