Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF)

Lenni Indriani; Mochammad Dharmautama; Edy Machmud; Muhammad Natsir Djide; Mochammad Hatta

doi:doi:10.11648/j.ajcem.20160403.14

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Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF)

Lenni Indriani, Mochammad Dharmautama, Edy Machmud, Muhammad Natsir Djide, Mochammad Hatta

Published in American Journal of Clinical and Experimental Medicine (Volume 4, Issue 3)

Received: 24 March 2016 Accepted: 16 April 2016 Published: 11 May 2016

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Abstract

Background: This study is performed to determine the effect of roselle extract gel 10% toward the changing of MMP - 8 mRNA expression in Gingival Creviculer Fluid (GCF) in patient with gingivitis experience post acrylic crown insertion. Methods: This study is a pre and post test experimental research. Research subjects were 9 patients who experience gingivitis post acrylic crown insertion and divided in three groups (treatment with roselle gel, negative control, and positive control). GCF samples were taken with paper strips before and seven days after application of roselle extract gel 10%. The change of MMP-8 mRNA expression is tested using Real Time-Polymerase Chain Reaction (RT-PCR) method. The results then statistically tested using paired T test to see the effect of rosella extract toward MMP-8 activity. Result: The MMP - 8 mRNA expression in gingivitis post acrylic crown insertion crown in the experimental group is significantly decreased after application of roselle extract gel 10%, which also seen in the positive control group, but no changes observed in the negative control group. Conclusions: Roselle extract gel 10% is proven to be effective in reducing the activity of MMP - 8 in GCF.

Published in	American Journal of Clinical and Experimental Medicine (Volume 4, Issue 3)
DOI	10.11648/j.ajcem.20160403.14
Page(s)	56-62
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Roselle Extract Gel, MMP - 8, Gingivitis, Acrylic Crown

References

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[4]	El Mowaf, O., Gingiva response to crown: 3 year report. JCDA. 2007; 73: 10.
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[6]	Ababnaeh K. T., Al-Omari M, Alawneh T. N., The effect of dental restoration type and material on periodontal health. Oral Health Prev Dent. 2011; 9 (4): 395-403.
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[8]	Rosenberg G. A., Matrix metalloproteinase and their multiple roles in neurodegenerative diseases. Lancet Neurol. 2009; 8: 205-16.
[9]	Sorsa T., Hernandez M., Leppilahti., Netuschii L., Mantyla P. Detection of gingiva crevicular fluid MMP-8 levels with different laboratory and chair-shide method. Oral Dis. Jan. 2010; 16 (1): 39-45.
[10]	Boom. R., Sol C. J. A., Salimans M. M. M, Jansen C. L., Wetheim-vanillen P. M. E., van der Noordaa J. Rapid and Sample Methode for Purification of Nucleid Acid. 1990; 28: 495-03.
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[13]	Mantyla P., Stenman M., Kinane D. F., Tikanoja S., Louto H., Solo T., et al. Gingiva Crevicular fluid collagenase-2 (MMP-8) test stick for chair-side monitoring of periodontits. J. Periodontal Research. 2003; 38: 436-9.
[14]	Mungole A., Chaturvedi A. Hibiscus Sabdariffa L A Rich Source Of Secondary Metabolites. International Journal of Pharmaceutical Sciences Review and Research. 2011. January – February 6 (1); Article-018.
[15]	Meraiyebu A. B, Olaniyan O. T, Eneze C., Anjorin Y. D., Dare J. B. Anti-inflammatory Activity of Methanolic Extract of Hibiscus sabdariffa on Carrageenan Induced Inflammation in Wistar Rat. International Journal of Pharmaceutical Science Invention. 2013; 2 (3): 22-4.
[16]	Sarkar, M. d. R, Hossen S. M. M., Howlader, S. I., Rahman, Md. A,. Dey A., Anti-diarrheal, analgesic, and anti-microbial activities of the plant Lalmesta (Hibiscus Sabdarifah): a review. International Journal of Pharmacological and Life Sciences. 2012: November; 1 (3).
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APA Style

Lenni Indriani, Mochammad Dharmautama, Edy Machmud, Muhammad Natsir Djide, Mochammad Hatta. (2016). Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF). American Journal of Clinical and Experimental Medicine, 4(3), 56-62. https://doi.org/10.11648/j.ajcem.20160403.14

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ACS Style

Lenni Indriani; Mochammad Dharmautama; Edy Machmud; Muhammad Natsir Djide; Mochammad Hatta. Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF). Am. J. Clin. Exp. Med. 2016, 4(3), 56-62. doi: 10.11648/j.ajcem.20160403.14

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AMA Style

Lenni Indriani, Mochammad Dharmautama, Edy Machmud, Muhammad Natsir Djide, Mochammad Hatta. Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF). Am J Clin Exp Med. 2016;4(3):56-62. doi: 10.11648/j.ajcem.20160403.14

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@article{10.11648/j.ajcem.20160403.14,
  author = {Lenni Indriani and Mochammad Dharmautama and Edy Machmud and Muhammad Natsir Djide and Mochammad Hatta},
  title = {Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF)},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {4},
  number = {3},
  pages = {56-62},
  doi = {10.11648/j.ajcem.20160403.14},
  url = {https://doi.org/10.11648/j.ajcem.20160403.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20160403.14},
  abstract = {Background: This study is performed to determine the effect of roselle extract gel 10% toward the changing of MMP - 8 mRNA expression in Gingival Creviculer Fluid (GCF) in patient with gingivitis experience post acrylic crown insertion. Methods: This study is a pre and post test experimental research. Research subjects were 9 patients who experience gingivitis post acrylic crown insertion and divided in three groups (treatment with roselle gel, negative control, and positive control). GCF samples were taken with paper strips before and seven days after application of roselle extract gel 10%. The change of MMP-8 mRNA expression is tested using Real Time-Polymerase Chain Reaction (RT-PCR) method. The results then statistically tested using paired T test to see the effect of rosella extract toward MMP-8 activity. Result: The MMP - 8 mRNA expression in gingivitis post acrylic crown insertion crown in the experimental group is significantly decreased after application of roselle extract gel 10%, which also seen in the positive control group, but no changes observed in the negative control group. Conclusions: Roselle extract gel 10% is proven to be effective in reducing the activity of MMP - 8 in GCF.},
 year = {2016}
}

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TY  - JOUR
T1  - Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF)
AU  - Lenni Indriani
AU  - Mochammad Dharmautama
AU  - Edy Machmud
AU  - Muhammad Natsir Djide
AU  - Mochammad Hatta
Y1  - 2016/05/11
PY  - 2016
N1  - https://doi.org/10.11648/j.ajcem.20160403.14
DO  - 10.11648/j.ajcem.20160403.14
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 56
EP  - 62
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20160403.14
AB  - Background: This study is performed to determine the effect of roselle extract gel 10% toward the changing of MMP - 8 mRNA expression in Gingival Creviculer Fluid (GCF) in patient with gingivitis experience post acrylic crown insertion. Methods: This study is a pre and post test experimental research. Research subjects were 9 patients who experience gingivitis post acrylic crown insertion and divided in three groups (treatment with roselle gel, negative control, and positive control). GCF samples were taken with paper strips before and seven days after application of roselle extract gel 10%. The change of MMP-8 mRNA expression is tested using Real Time-Polymerase Chain Reaction (RT-PCR) method. The results then statistically tested using paired T test to see the effect of rosella extract toward MMP-8 activity. Result: The MMP - 8 mRNA expression in gingivitis post acrylic crown insertion crown in the experimental group is significantly decreased after application of roselle extract gel 10%, which also seen in the positive control group, but no changes observed in the negative control group. Conclusions: Roselle extract gel 10% is proven to be effective in reducing the activity of MMP - 8 in GCF.
VL  - 4
IS  - 3
ER  -

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Author Information

Lenni Indriani

Department of Dental Material, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia
Mochammad Dharmautama

Department of Prosthodontic, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia
Edy Machmud

Department of Prosthodontic, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia
Muhammad Natsir Djide

Department of Microbiology and biotechnology, Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia
Mochammad Hatta

Molecular Biology and Immunology Laboratory, Departement of Microbiology. Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

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APA Style

Lenni Indriani, Mochammad Dharmautama, Edy Machmud, Muhammad Natsir Djide, Mochammad Hatta. (2016). Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF). American Journal of Clinical and Experimental Medicine, 4(3), 56-62. https://doi.org/10.11648/j.ajcem.20160403.14

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ACS Style

Lenni Indriani; Mochammad Dharmautama; Edy Machmud; Muhammad Natsir Djide; Mochammad Hatta. Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF). Am. J. Clin. Exp. Med. 2016, 4(3), 56-62. doi: 10.11648/j.ajcem.20160403.14

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AMA Style

Lenni Indriani, Mochammad Dharmautama, Edy Machmud, Muhammad Natsir Djide, Mochammad Hatta. Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF). Am J Clin Exp Med. 2016;4(3):56-62. doi: 10.11648/j.ajcem.20160403.14

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@article{10.11648/j.ajcem.20160403.14,
  author = {Lenni Indriani and Mochammad Dharmautama and Edy Machmud and Muhammad Natsir Djide and Mochammad Hatta},
  title = {Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF)},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {4},
  number = {3},
  pages = {56-62},
  doi = {10.11648/j.ajcem.20160403.14},
  url = {https://doi.org/10.11648/j.ajcem.20160403.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20160403.14},
  abstract = {Background: This study is performed to determine the effect of roselle extract gel 10% toward the changing of MMP - 8 mRNA expression in Gingival Creviculer Fluid (GCF) in patient with gingivitis experience post acrylic crown insertion. Methods: This study is a pre and post test experimental research. Research subjects were 9 patients who experience gingivitis post acrylic crown insertion and divided in three groups (treatment with roselle gel, negative control, and positive control). GCF samples were taken with paper strips before and seven days after application of roselle extract gel 10%. The change of MMP-8 mRNA expression is tested using Real Time-Polymerase Chain Reaction (RT-PCR) method. The results then statistically tested using paired T test to see the effect of rosella extract toward MMP-8 activity. Result: The MMP - 8 mRNA expression in gingivitis post acrylic crown insertion crown in the experimental group is significantly decreased after application of roselle extract gel 10%, which also seen in the positive control group, but no changes observed in the negative control group. Conclusions: Roselle extract gel 10% is proven to be effective in reducing the activity of MMP - 8 in GCF.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Effect of Roselle Extract in Expression of Matrix Metallo Proteinase - 8 (MMP-8) in Gingival Creviculer Fluid (GCF)
AU  - Lenni Indriani
AU  - Mochammad Dharmautama
AU  - Edy Machmud
AU  - Muhammad Natsir Djide
AU  - Mochammad Hatta
Y1  - 2016/05/11
PY  - 2016
N1  - https://doi.org/10.11648/j.ajcem.20160403.14
DO  - 10.11648/j.ajcem.20160403.14
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 56
EP  - 62
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20160403.14
AB  - Background: This study is performed to determine the effect of roselle extract gel 10% toward the changing of MMP - 8 mRNA expression in Gingival Creviculer Fluid (GCF) in patient with gingivitis experience post acrylic crown insertion. Methods: This study is a pre and post test experimental research. Research subjects were 9 patients who experience gingivitis post acrylic crown insertion and divided in three groups (treatment with roselle gel, negative control, and positive control). GCF samples were taken with paper strips before and seven days after application of roselle extract gel 10%. The change of MMP-8 mRNA expression is tested using Real Time-Polymerase Chain Reaction (RT-PCR) method. The results then statistically tested using paired T test to see the effect of rosella extract toward MMP-8 activity. Result: The MMP - 8 mRNA expression in gingivitis post acrylic crown insertion crown in the experimental group is significantly decreased after application of roselle extract gel 10%, which also seen in the positive control group, but no changes observed in the negative control group. Conclusions: Roselle extract gel 10% is proven to be effective in reducing the activity of MMP - 8 in GCF.
VL  - 4
IS  - 3
ER  -

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