Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease

Redento Mora; Diana Binanti; Nicoletta Mora; Eleonora Fantinato; Valentina Ferrante; Luisella Pedrotti; Pietro Riccaboni

doi:doi:10.11648/j.ajcem.20150304.18

Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease

Redento Mora, Diana Binanti, Nicoletta Mora, Eleonora Fantinato, Valentina Ferrante, Luisella Pedrotti, Pietro Riccaboni

Published in American Journal of Clinical and Experimental Medicine (Volume 3, Issue 4)

Received: 25 May 2015 Accepted: 9 June 2015 Published: 25 June 2015

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Abstract

Background: MMP-2 and MMP-9 are proteolytic enzymes involved in the remodeling of extracellular matrix and regulated in their function by a family of proteins called TIMPs. MMP-2 and MMP-9 and TIMPs are secreted in joints by both synovial cells and chondrocytes, and are implicated in osteoarthritis onset and progression. The aim of this study was to investigate the localization of MMP-2 and TIMPs expression and their relationship in the synovial membrane of equine fetlock (metacarpo-phalangeal joint), which is the joint most frequently affected by osteoarthritis due to his high weight-bearing impact and shearing forces. Method: This study analyzed the immunohistochemical expression of MMP-2 and tissue inhibitors of MMP (TIMPs) in synovial membrane sections from 40 equine metacarpo-phalangeal joints affected by osteoarthritis in order to better assess their role in the pathogenesis of joint-structure alterations. Immunohistochemical study was performed using the Avidin-Biotin-Peroxidase complex method, and the percentage of labeled cells was assessed semi-quantitatively. Data obtained were statistically revised through the Spearman's rank correlation coefficient. Results: MMP-2 and TIMPs were expressed by type-B synoviocytes and endothelial cells. MMP-2 expression was not significantly associated to the macroscopic findings while a linear correlation between MMP-2 and TIMPs expression was observed. Conclusion: These data suggest that in degenerative joint disease the synovial expression of TIMPs may contribute to the regulation of MMP-2 expression in order to reach a new dynamic balance and that MMP-2 plays an important pro-homeostatic role in physiological and pathological equine synovial membrane.

Published in	American Journal of Clinical and Experimental Medicine (Volume 3, Issue 4)
DOI	10.11648/j.ajcem.20150304.18
Page(s)	172-177
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

MMPs, TIMPs, Fetlock, Horse, Immunohistochemistry

References

[1]	Boveland S., Moore P., Mysore J., Krunkosky T.M., Dietrich U.M., Jarrett C., Paige Carmichael K.: Immunohistochemical study of matrix metalloproteinases-2 and -9, macrophage inflammatory protein-2 and tissue inhibitors of matrix metalloproteinases-1 and -2 in normal, purulonecrotic and fungal infected equine corneas. Vet Ophtalmol 2010, 13: 81-90.
[2]	Brew K., Dinakarpandian D., Nagase H.: Tissue inhibitors of metalloproteinases: evolution, structure and function. Biochim Biophys Acta 2000, 1477: 267-283.
[3]	Carmona J.U., Prades M.: Pathophysiology of Osteoarthritis. Compendium Equine.com 2009, January/February: 28-40.
[4]	Clegg P.D., Carter S.D.: Matrix metalloproteinase -2 and -9 are activated in joint diseases. Equine Vet J 1999, 31: 324-330.
[5]	Clegg P.D., Coughlan A.R., Carter S.D.: Equine TIMP-1 and TIMP-2: Identification, activity and cellular sources. Equine Vet J 1998, 30: 416-423.
[6]	Clegg P.D., Coughlan A.R., Riggs C.M., Carter S.D.:Matrix metalloproteinases 2 and 9 in equine synovial fluids. Equine Vet J 1997, 29: 343-348.
[7]	Clutterbuck A., Harris P., Allaway D., Mobasheri A.: Matrix metalloproteinases in inflammatory pathologies of the horse. Vet J 2010, 108: 27-38.
[8]	Curran S., Murray G.I.: Matrix metalloproteinases in tumour invasion and metastasis. J Pathol 1999, 189: 300-308.
[9]	Fassina G., Ferrari N., Brigati C., Benelli R., Santi L. Noonan D.M., Albini A.: Tissue inhibitors of metalloproteases: Regulation and biological activities. Clin Exp Metastasis 2000: 18: 111-120.
[10]	Fietz S., Einspanier R., Hoppner S., Hertsch B., Bondzio A.: Determination of MMP-2 and -9 activities in synovial fluids of horses with osteoarhritic and arthritic joint diseases using gelatin zymography and immunocapture activity assays. Equine Vet J 2008, 40: 266-271.
[11]	Kevorkian L., Young D.A., Darrah C., Donell S.T., Shepstone L., Porter S. et al.: Expression profiling of metalloproteinases and their inhibitors in cartilage. Arthritis Rheum 2004, 50: 131-141.
[12]	Kyaw-Tanner M.T., Wattle O., van Eps A.W., Pollitt C.C.: Equine laminitis: Membrane type matrix metalloproteinase-1 (MMP-14) is involved in acute phase onset. Equine Vet J 2008,40: 482-487.
[13]	Lambert E., Dassé E., Haye B., Petitfrère E.: TIMPs as multifacial proteins. Critical Rev Oncol Hematology 2004, 49: 187-198.
[14]	Murphy G., Lee M.H.: What are the roles of metalloproteinases in cartilage and bone damage? Ann Rheum Dis 2005, 64: 44-47.
[15]	Nagase H., Visse R., Murphy G.: Structure and function of matrix metalloproteinases and TIMPs. Cardiovasc Res 2006, 69: 562-573.
[16]	Sbardella D., Fasciglione G.F., Gioia M., Ciaccio C., Tundo G.R., Marini S., Coletta M.: Human matrix metalloproteinases: An ubiquitarian class of enzymes involved in several pathological processes. A review. Mol Aspects Med 2012, 33: 119-208.
[17]	Tanaka A., Kawashiri S., Kumagai S., Takatsuka S., Narinobou M., Nakagawa, K. et al.: Expression of matrix metalloproteinase-2 in osteoarthritic fibrocartilage from human mandibular condyle. J Oral Pathol Med 2000, 9: 314-320.
[18]	Tetlow L.C., Daman J.A., Woolley D.E.: Matrix metalloproteinase and proinflammatory cytokine production by chondrocytes of human osteoarthritic cartilage. Arthritis Rheum 2001, 44: 585-594.
[19]	Van den Steen P.E., Dubois B., Nelissen I., Rudd P.M., Dwek R.A., Opdenakker G.: Biochemistry and molecular biology of gelatinase B or matrix metalloproteinase-9 (MMP-9). Crit Rev Biochem Mol Biol 2002, 37: 375-536.
[20]	Visse R,, Nagase H.: Matrix Metalloproteinases and Tissue inhibitors of Metalloproteinases: Structure, Function and Biochemistry. Circ Res 2012, 92: 827-839.
[21]	Galasso O., Familiari F., De Gori M., Gasparini G.: Recent findings on the role of gelatinases (Matrix Metalloproteinases -2 and -9) in osteoarthritis. Adv Orthop 2012 ID 834208: 1-7.
[22]	Murphy G., Nagase H.: Reappraising metalloproteinases in rheumatoid arthritis and osteoarthritis; destruction or repair? Nature Clin Pract Rheumatol 2008, 4: 128-135.

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APA Style

Redento Mora, Diana Binanti, Nicoletta Mora, Eleonora Fantinato, Valentina Ferrante, et al. (2015). Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease. American Journal of Clinical and Experimental Medicine, 3(4), 172-177. https://doi.org/10.11648/j.ajcem.20150304.18

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ACS Style

Redento Mora; Diana Binanti; Nicoletta Mora; Eleonora Fantinato; Valentina Ferrante, et al. Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease. Am. J. Clin. Exp. Med. 2015, 3(4), 172-177. doi: 10.11648/j.ajcem.20150304.18

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AMA Style

Redento Mora, Diana Binanti, Nicoletta Mora, Eleonora Fantinato, Valentina Ferrante, et al. Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease. Am J Clin Exp Med. 2015;3(4):172-177. doi: 10.11648/j.ajcem.20150304.18

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@article{10.11648/j.ajcem.20150304.18,
  author = {Redento Mora and Diana Binanti and Nicoletta Mora and Eleonora Fantinato and Valentina Ferrante and Luisella Pedrotti and Pietro Riccaboni},
  title = {Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {4},
  pages = {172-177},
  doi = {10.11648/j.ajcem.20150304.18},
  url = {https://doi.org/10.11648/j.ajcem.20150304.18},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150304.18},
  abstract = {Background: MMP-2 and MMP-9 are proteolytic enzymes involved in the remodeling of extracellular matrix and regulated in their function by a family of proteins called TIMPs. MMP-2 and MMP-9 and TIMPs are secreted in joints by both synovial cells and chondrocytes, and are implicated in osteoarthritis onset and progression. The aim of this study was to investigate the localization of MMP-2 and TIMPs expression and their relationship in the synovial membrane of equine fetlock (metacarpo-phalangeal joint), which is the joint most frequently affected by osteoarthritis due to his high weight-bearing impact and shearing forces. Method: This study analyzed the immunohistochemical expression of MMP-2 and tissue inhibitors of MMP (TIMPs) in synovial membrane sections from 40 equine metacarpo-phalangeal joints affected by osteoarthritis in order to better assess their role in the pathogenesis of joint-structure alterations. Immunohistochemical study was performed using the Avidin-Biotin-Peroxidase complex method, and the percentage of labeled cells was assessed semi-quantitatively. Data obtained were statistically revised through the Spearman's rank correlation coefficient. Results: MMP-2 and TIMPs were expressed by type-B synoviocytes and endothelial cells. MMP-2 expression was not significantly associated to the macroscopic findings while a linear correlation between MMP-2 and TIMPs expression was observed. Conclusion: These data suggest that in degenerative joint disease the synovial expression of TIMPs may contribute to the regulation of MMP-2 expression in order to reach a new dynamic balance and that MMP-2 plays an important pro-homeostatic role in physiological and pathological equine synovial membrane.},
 year = {2015}
}

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TY  - JOUR
T1  - Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease
AU  - Redento Mora
AU  - Diana Binanti
AU  - Nicoletta Mora
AU  - Eleonora Fantinato
AU  - Valentina Ferrante
AU  - Luisella Pedrotti
AU  - Pietro Riccaboni
Y1  - 2015/06/25
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150304.18
DO  - 10.11648/j.ajcem.20150304.18
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 172
EP  - 177
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150304.18
AB  - Background: MMP-2 and MMP-9 are proteolytic enzymes involved in the remodeling of extracellular matrix and regulated in their function by a family of proteins called TIMPs. MMP-2 and MMP-9 and TIMPs are secreted in joints by both synovial cells and chondrocytes, and are implicated in osteoarthritis onset and progression. The aim of this study was to investigate the localization of MMP-2 and TIMPs expression and their relationship in the synovial membrane of equine fetlock (metacarpo-phalangeal joint), which is the joint most frequently affected by osteoarthritis due to his high weight-bearing impact and shearing forces. Method: This study analyzed the immunohistochemical expression of MMP-2 and tissue inhibitors of MMP (TIMPs) in synovial membrane sections from 40 equine metacarpo-phalangeal joints affected by osteoarthritis in order to better assess their role in the pathogenesis of joint-structure alterations. Immunohistochemical study was performed using the Avidin-Biotin-Peroxidase complex method, and the percentage of labeled cells was assessed semi-quantitatively. Data obtained were statistically revised through the Spearman's rank correlation coefficient. Results: MMP-2 and TIMPs were expressed by type-B synoviocytes and endothelial cells. MMP-2 expression was not significantly associated to the macroscopic findings while a linear correlation between MMP-2 and TIMPs expression was observed. Conclusion: These data suggest that in degenerative joint disease the synovial expression of TIMPs may contribute to the regulation of MMP-2 expression in order to reach a new dynamic balance and that MMP-2 plays an important pro-homeostatic role in physiological and pathological equine synovial membrane.
VL  - 3
IS  - 4
ER  -

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Author Information

Redento Mora

Orthopaedic Section
Diana Binanti

Department of Veterinary Science and Public Health, University of Milan, Veterinary University Hospital, School of Veterinary Medicine, Lodi, Italy
Nicoletta Mora

Department of Veterinary Science and Public Health, University of Milan, Veterinary University Hospital, School of Veterinary Medicine, Lodi, Italy
Eleonora Fantinato

Department of Veterinary Science and Public Health, University of Milan, Veterinary University Hospital, School of Veterinary Medicine, Lodi, Italy
Valentina Ferrante

Department of Veterinary Science and Public Health, University of Milan, Veterinary University Hospital, School of Veterinary Medicine, Lodi, Italy
Luisella Pedrotti

Orthopaedic Section
Pietro Riccaboni

Department of Veterinary Science and Public Health, University of Milan, Veterinary University Hospital, School of Veterinary Medicine, Lodi, Italy

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Plain Text BibTeX RIS

APA Style

Redento Mora, Diana Binanti, Nicoletta Mora, Eleonora Fantinato, Valentina Ferrante, et al. (2015). Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease. American Journal of Clinical and Experimental Medicine, 3(4), 172-177. https://doi.org/10.11648/j.ajcem.20150304.18

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ACS Style

Redento Mora; Diana Binanti; Nicoletta Mora; Eleonora Fantinato; Valentina Ferrante, et al. Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease. Am. J. Clin. Exp. Med. 2015, 3(4), 172-177. doi: 10.11648/j.ajcem.20150304.18

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AMA Style

Redento Mora, Diana Binanti, Nicoletta Mora, Eleonora Fantinato, Valentina Ferrante, et al. Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease. Am J Clin Exp Med. 2015;3(4):172-177. doi: 10.11648/j.ajcem.20150304.18

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@article{10.11648/j.ajcem.20150304.18,
  author = {Redento Mora and Diana Binanti and Nicoletta Mora and Eleonora Fantinato and Valentina Ferrante and Luisella Pedrotti and Pietro Riccaboni},
  title = {Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {4},
  pages = {172-177},
  doi = {10.11648/j.ajcem.20150304.18},
  url = {https://doi.org/10.11648/j.ajcem.20150304.18},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150304.18},
  abstract = {Background: MMP-2 and MMP-9 are proteolytic enzymes involved in the remodeling of extracellular matrix and regulated in their function by a family of proteins called TIMPs. MMP-2 and MMP-9 and TIMPs are secreted in joints by both synovial cells and chondrocytes, and are implicated in osteoarthritis onset and progression. The aim of this study was to investigate the localization of MMP-2 and TIMPs expression and their relationship in the synovial membrane of equine fetlock (metacarpo-phalangeal joint), which is the joint most frequently affected by osteoarthritis due to his high weight-bearing impact and shearing forces. Method: This study analyzed the immunohistochemical expression of MMP-2 and tissue inhibitors of MMP (TIMPs) in synovial membrane sections from 40 equine metacarpo-phalangeal joints affected by osteoarthritis in order to better assess their role in the pathogenesis of joint-structure alterations. Immunohistochemical study was performed using the Avidin-Biotin-Peroxidase complex method, and the percentage of labeled cells was assessed semi-quantitatively. Data obtained were statistically revised through the Spearman's rank correlation coefficient. Results: MMP-2 and TIMPs were expressed by type-B synoviocytes and endothelial cells. MMP-2 expression was not significantly associated to the macroscopic findings while a linear correlation between MMP-2 and TIMPs expression was observed. Conclusion: These data suggest that in degenerative joint disease the synovial expression of TIMPs may contribute to the regulation of MMP-2 expression in order to reach a new dynamic balance and that MMP-2 plays an important pro-homeostatic role in physiological and pathological equine synovial membrane.},
 year = {2015}
}

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TY  - JOUR
T1  - Pathological Findings and Immunohistochemical Evaluation of MMP-2 and TIMPs in Equine Fetlock Affected by Degenerative Joint Disease
AU  - Redento Mora
AU  - Diana Binanti
AU  - Nicoletta Mora
AU  - Eleonora Fantinato
AU  - Valentina Ferrante
AU  - Luisella Pedrotti
AU  - Pietro Riccaboni
Y1  - 2015/06/25
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150304.18
DO  - 10.11648/j.ajcem.20150304.18
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 172
EP  - 177
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150304.18
AB  - Background: MMP-2 and MMP-9 are proteolytic enzymes involved in the remodeling of extracellular matrix and regulated in their function by a family of proteins called TIMPs. MMP-2 and MMP-9 and TIMPs are secreted in joints by both synovial cells and chondrocytes, and are implicated in osteoarthritis onset and progression. The aim of this study was to investigate the localization of MMP-2 and TIMPs expression and their relationship in the synovial membrane of equine fetlock (metacarpo-phalangeal joint), which is the joint most frequently affected by osteoarthritis due to his high weight-bearing impact and shearing forces. Method: This study analyzed the immunohistochemical expression of MMP-2 and tissue inhibitors of MMP (TIMPs) in synovial membrane sections from 40 equine metacarpo-phalangeal joints affected by osteoarthritis in order to better assess their role in the pathogenesis of joint-structure alterations. Immunohistochemical study was performed using the Avidin-Biotin-Peroxidase complex method, and the percentage of labeled cells was assessed semi-quantitatively. Data obtained were statistically revised through the Spearman's rank correlation coefficient. Results: MMP-2 and TIMPs were expressed by type-B synoviocytes and endothelial cells. MMP-2 expression was not significantly associated to the macroscopic findings while a linear correlation between MMP-2 and TIMPs expression was observed. Conclusion: These data suggest that in degenerative joint disease the synovial expression of TIMPs may contribute to the regulation of MMP-2 expression in order to reach a new dynamic balance and that MMP-2 plays an important pro-homeostatic role in physiological and pathological equine synovial membrane.
VL  - 3
IS  - 4
ER  -

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