The Analysis of Vascular Endothelial Growth Factor Gene Polymorphisms on Clinical and Histopathology Features of Nasopharyngeal Carcinoma

Abdul Qadar Punagi

doi:doi:10.11648/j.ajcem.20150304.15

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The Analysis of Vascular Endothelial Growth Factor Gene Polymorphisms on Clinical and Histopathology Features of Nasopharyngeal Carcinoma

Abdul Qadar Punagi

Published in American Journal of Clinical and Experimental Medicine (Volume 3, Issue 4)

Received: 20 May 2015 Accepted: 3 June 2015 Published: 19 June 2015

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Abstract

Background: Nasopharyngeal malignancy is the first most common malignancy amongst in ear, nose and throat. Incidence rate was 4.7 per 100.000 or 7.000-8.000 cases per year and most of carcinoma is arising from the epithelial cells. Objective: To analyze the VEGF gene variations at +405 C/G and -460 T/C with VEGFR (Flt-4) and LMP-1 tissues expression of nasopharyngeal carcinoma (NPC). Methods: A cross sectional study was carried out with explorative approached at several teaching hospitals in Makassar by Hasanuddin University Research Centre and Eijkman Institute for Molecular Biology on a one-year period, from July 2006 through August 2007. The analysis covered from 90 samples of blood and 45 samples of nasopharynx tissue, consisting of 45 patients for both of the NPC and without NPC as a control. Genomic DNA was extracted from peripheral blood and analyzed by PCR and direct DNA sequencing method for identifying the location of VEGF gene mutation and immunohistochemical expression of VEGFR (Flt-4) and LMP-1 were performed in 45 NPC biopsy samples with avidin-biotin method. Results: The frequencies of +405 C/G and -460 T/C were about 50%, with higher in +405 C/G (hot spot), mostly genotype variant was heterozygote (CG). Our results confirmed that untranslated and promoter region of VEGF gene were higher polymorphic. GC and CC haplotype at +405 C/G and -460 T/C of VEGF gene more susceptible to NPC compared with CT haplotype but no statistical significant. Conclusion: There were no relationships between genotype distribution and allele frequencies at VEGF gene +405 C/G, -460 T/C and -457 T/C with the NPC risk factors.

Published in	American Journal of Clinical and Experimental Medicine (Volume 3, Issue 4)
DOI	10.11648/j.ajcem.20150304.15
Page(s)	154-161
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Nasopharyngeal Carcinoma (NPC), Vascular Endothelial Growth Factor (VEGF), Latent Membrane Protein (LMP-1)

References

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APA Style

Abdul Qadar Punagi. (2015). The Analysis of Vascular Endothelial Growth Factor Gene Polymorphisms on Clinical and Histopathology Features of Nasopharyngeal Carcinoma. American Journal of Clinical and Experimental Medicine, 3(4), 154-161. https://doi.org/10.11648/j.ajcem.20150304.15

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ACS Style

Abdul Qadar Punagi. The Analysis of Vascular Endothelial Growth Factor Gene Polymorphisms on Clinical and Histopathology Features of Nasopharyngeal Carcinoma. Am. J. Clin. Exp. Med. 2015, 3(4), 154-161. doi: 10.11648/j.ajcem.20150304.15

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AMA Style

Abdul Qadar Punagi. The Analysis of Vascular Endothelial Growth Factor Gene Polymorphisms on Clinical and Histopathology Features of Nasopharyngeal Carcinoma. Am J Clin Exp Med. 2015;3(4):154-161. doi: 10.11648/j.ajcem.20150304.15

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@article{10.11648/j.ajcem.20150304.15,
  author = {Abdul Qadar Punagi},
  title = {The Analysis of Vascular Endothelial Growth Factor Gene Polymorphisms on Clinical and Histopathology Features of Nasopharyngeal Carcinoma},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {4},
  pages = {154-161},
  doi = {10.11648/j.ajcem.20150304.15},
  url = {https://doi.org/10.11648/j.ajcem.20150304.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150304.15},
  abstract = {Background: Nasopharyngeal malignancy is the first most common malignancy amongst in ear, nose and throat. Incidence rate was 4.7 per 100.000 or 7.000-8.000 cases per year and most of carcinoma is arising from the epithelial cells. Objective: To analyze the VEGF gene variations at +405 C/G and -460 T/C with VEGFR (Flt-4) and LMP-1 tissues expression of nasopharyngeal carcinoma (NPC). Methods: A cross sectional study was carried out with explorative approached at several teaching hospitals in Makassar by Hasanuddin University Research Centre and Eijkman Institute for Molecular Biology on a one-year period, from July 2006 through August 2007. The analysis covered from 90 samples of blood and 45 samples of nasopharynx tissue, consisting of 45 patients for both of the NPC and without NPC as a control. Genomic DNA was extracted from peripheral blood and analyzed by PCR and direct DNA sequencing method for identifying the location of VEGF gene mutation and immunohistochemical expression of VEGFR (Flt-4) and LMP-1 were performed in 45 NPC biopsy samples with avidin-biotin method. Results: The frequencies of +405 C/G and -460 T/C were about 50%, with higher in +405 C/G (hot spot), mostly genotype variant was heterozygote (CG). Our results confirmed that untranslated and promoter region of VEGF gene were higher polymorphic. GC and CC haplotype at +405 C/G and -460 T/C of VEGF gene more susceptible to NPC compared with CT haplotype but no statistical significant. Conclusion: There were no relationships between genotype distribution and allele frequencies at VEGF gene +405 C/G, -460 T/C and -457 T/C with the NPC risk factors.},
 year = {2015}
}

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TY  - JOUR
T1  - The Analysis of Vascular Endothelial Growth Factor Gene Polymorphisms on Clinical and Histopathology Features of Nasopharyngeal Carcinoma
AU  - Abdul Qadar Punagi
Y1  - 2015/06/19
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150304.15
DO  - 10.11648/j.ajcem.20150304.15
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 154
EP  - 161
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150304.15
AB  - Background: Nasopharyngeal malignancy is the first most common malignancy amongst in ear, nose and throat. Incidence rate was 4.7 per 100.000 or 7.000-8.000 cases per year and most of carcinoma is arising from the epithelial cells. Objective: To analyze the VEGF gene variations at +405 C/G and -460 T/C with VEGFR (Flt-4) and LMP-1 tissues expression of nasopharyngeal carcinoma (NPC). Methods: A cross sectional study was carried out with explorative approached at several teaching hospitals in Makassar by Hasanuddin University Research Centre and Eijkman Institute for Molecular Biology on a one-year period, from July 2006 through August 2007. The analysis covered from 90 samples of blood and 45 samples of nasopharynx tissue, consisting of 45 patients for both of the NPC and without NPC as a control. Genomic DNA was extracted from peripheral blood and analyzed by PCR and direct DNA sequencing method for identifying the location of VEGF gene mutation and immunohistochemical expression of VEGFR (Flt-4) and LMP-1 were performed in 45 NPC biopsy samples with avidin-biotin method. Results: The frequencies of +405 C/G and -460 T/C were about 50%, with higher in +405 C/G (hot spot), mostly genotype variant was heterozygote (CG). Our results confirmed that untranslated and promoter region of VEGF gene were higher polymorphic. GC and CC haplotype at +405 C/G and -460 T/C of VEGF gene more susceptible to NPC compared with CT haplotype but no statistical significant. Conclusion: There were no relationships between genotype distribution and allele frequencies at VEGF gene +405 C/G, -460 T/C and -457 T/C with the NPC risk factors.
VL  - 3
IS  - 4
ER  -

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Author Information

Abdul Qadar Punagi

Department of Otorhinolaryngology, Medical Faculty of Hasanuddin University, Makassar, Indonesia

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APA Style

Abdul Qadar Punagi. (2015). The Analysis of Vascular Endothelial Growth Factor Gene Polymorphisms on Clinical and Histopathology Features of Nasopharyngeal Carcinoma. American Journal of Clinical and Experimental Medicine, 3(4), 154-161. https://doi.org/10.11648/j.ajcem.20150304.15

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ACS Style

Abdul Qadar Punagi. The Analysis of Vascular Endothelial Growth Factor Gene Polymorphisms on Clinical and Histopathology Features of Nasopharyngeal Carcinoma. Am. J. Clin. Exp. Med. 2015, 3(4), 154-161. doi: 10.11648/j.ajcem.20150304.15

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AMA Style

Abdul Qadar Punagi. The Analysis of Vascular Endothelial Growth Factor Gene Polymorphisms on Clinical and Histopathology Features of Nasopharyngeal Carcinoma. Am J Clin Exp Med. 2015;3(4):154-161. doi: 10.11648/j.ajcem.20150304.15

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@article{10.11648/j.ajcem.20150304.15,
  author = {Abdul Qadar Punagi},
  title = {The Analysis of Vascular Endothelial Growth Factor Gene Polymorphisms on Clinical and Histopathology Features of Nasopharyngeal Carcinoma},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {4},
  pages = {154-161},
  doi = {10.11648/j.ajcem.20150304.15},
  url = {https://doi.org/10.11648/j.ajcem.20150304.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150304.15},
  abstract = {Background: Nasopharyngeal malignancy is the first most common malignancy amongst in ear, nose and throat. Incidence rate was 4.7 per 100.000 or 7.000-8.000 cases per year and most of carcinoma is arising from the epithelial cells. Objective: To analyze the VEGF gene variations at +405 C/G and -460 T/C with VEGFR (Flt-4) and LMP-1 tissues expression of nasopharyngeal carcinoma (NPC). Methods: A cross sectional study was carried out with explorative approached at several teaching hospitals in Makassar by Hasanuddin University Research Centre and Eijkman Institute for Molecular Biology on a one-year period, from July 2006 through August 2007. The analysis covered from 90 samples of blood and 45 samples of nasopharynx tissue, consisting of 45 patients for both of the NPC and without NPC as a control. Genomic DNA was extracted from peripheral blood and analyzed by PCR and direct DNA sequencing method for identifying the location of VEGF gene mutation and immunohistochemical expression of VEGFR (Flt-4) and LMP-1 were performed in 45 NPC biopsy samples with avidin-biotin method. Results: The frequencies of +405 C/G and -460 T/C were about 50%, with higher in +405 C/G (hot spot), mostly genotype variant was heterozygote (CG). Our results confirmed that untranslated and promoter region of VEGF gene were higher polymorphic. GC and CC haplotype at +405 C/G and -460 T/C of VEGF gene more susceptible to NPC compared with CT haplotype but no statistical significant. Conclusion: There were no relationships between genotype distribution and allele frequencies at VEGF gene +405 C/G, -460 T/C and -457 T/C with the NPC risk factors.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - The Analysis of Vascular Endothelial Growth Factor Gene Polymorphisms on Clinical and Histopathology Features of Nasopharyngeal Carcinoma
AU  - Abdul Qadar Punagi
Y1  - 2015/06/19
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150304.15
DO  - 10.11648/j.ajcem.20150304.15
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 154
EP  - 161
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150304.15
AB  - Background: Nasopharyngeal malignancy is the first most common malignancy amongst in ear, nose and throat. Incidence rate was 4.7 per 100.000 or 7.000-8.000 cases per year and most of carcinoma is arising from the epithelial cells. Objective: To analyze the VEGF gene variations at +405 C/G and -460 T/C with VEGFR (Flt-4) and LMP-1 tissues expression of nasopharyngeal carcinoma (NPC). Methods: A cross sectional study was carried out with explorative approached at several teaching hospitals in Makassar by Hasanuddin University Research Centre and Eijkman Institute for Molecular Biology on a one-year period, from July 2006 through August 2007. The analysis covered from 90 samples of blood and 45 samples of nasopharynx tissue, consisting of 45 patients for both of the NPC and without NPC as a control. Genomic DNA was extracted from peripheral blood and analyzed by PCR and direct DNA sequencing method for identifying the location of VEGF gene mutation and immunohistochemical expression of VEGFR (Flt-4) and LMP-1 were performed in 45 NPC biopsy samples with avidin-biotin method. Results: The frequencies of +405 C/G and -460 T/C were about 50%, with higher in +405 C/G (hot spot), mostly genotype variant was heterozygote (CG). Our results confirmed that untranslated and promoter region of VEGF gene were higher polymorphic. GC and CC haplotype at +405 C/G and -460 T/C of VEGF gene more susceptible to NPC compared with CT haplotype but no statistical significant. Conclusion: There were no relationships between genotype distribution and allele frequencies at VEGF gene +405 C/G, -460 T/C and -457 T/C with the NPC risk factors.
VL  - 3
IS  - 4
ER  -

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