Background: Lippia adoensis var. koseret is a well known medicinal plant endemic to Ethiopia. It has been traditionally used to treat different infectious diseases and also in food preparation as condiment. The aim of the current study was to evaluate antibacterial and antifungal activities of water, ethanol and methanol based crude extracts of L. adoensis var. koseret against selected human pathogenic bacteria and fungi. Methods: Crude extracts of L. adoensis var. koseret were extracted by maceration method. Disc diffusion assay of the extracts were carried out in four different concentrations against three different bacteria species (Staphylococcus aureus, Enterococcus faecalis and Escherichia coli) and two clinical isolated fungal species (Candida albicans and Aspergillus flavus) by using Kirby- Baur disk diffusion method. Agar dilution method was used to determine the minimum inhibitory concentration, the minimum bactericidal and fungicidal concentrations of the extracts against similar microorganisms. Results: Water-based extract of L. adoensis var. koseret exhibited significantly less antimicrobial activity as compared to ethanol and methanol crude extract against tested isolates of bacteria and fungi (P < 0.05); while, there was no significant difference between ethanol and methanol extracts. Among the tested microorganism S. aureus, was the most sensitive of all whereas C. albicans was the most resistant microorganism to alcohol based extract of L. adoensis var. koseret. The minimum inhibitory concentration of L. adoensis var. koseret ranged from 3.12 to 25mg/ml in the alcohol based extracts but it was higher in the water-based extract. The lower bactericidal concentration (5.20 mg/l) and the highest fungicidal concentration (37.50 mg/ml) were observed in methanol based extracts against S. aureus and C. albicans, respectively. Conclusions: Antimicrobial activity of L. adoensis var. koseret varies with extraction solvent and tested microorganisms.
| Published in | American Journal of Clinical and Experimental Medicine (Volume 3, Issue 3) |
| DOI | 10.11648/j.ajcem.20150303.18 |
| Page(s) | 118-123 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Antibacterial Activity, Antifungal Activity, Crude Extract, L. adoensis var. koseret
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APA Style
Gemechu Ameya Buli, Abdella Gure Duga, Engda Dessalegn. (2015). Antimicrobial Activity of Lippia adoensis var. koseret Against Human Pathogenic Bacteria and Fungi. American Journal of Clinical and Experimental Medicine, 3(3), 118-123. https://doi.org/10.11648/j.ajcem.20150303.18
ACS Style
Gemechu Ameya Buli; Abdella Gure Duga; Engda Dessalegn. Antimicrobial Activity of Lippia adoensis var. koseret Against Human Pathogenic Bacteria and Fungi. Am. J. Clin. Exp. Med. 2015, 3(3), 118-123. doi: 10.11648/j.ajcem.20150303.18
AMA Style
Gemechu Ameya Buli, Abdella Gure Duga, Engda Dessalegn. Antimicrobial Activity of Lippia adoensis var. koseret Against Human Pathogenic Bacteria and Fungi. Am J Clin Exp Med. 2015;3(3):118-123. doi: 10.11648/j.ajcem.20150303.18
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Download@article{10.11648/j.ajcem.20150303.18,
author = {Gemechu Ameya Buli and Abdella Gure Duga and Engda Dessalegn},
title = {Antimicrobial Activity of Lippia adoensis var. koseret Against Human Pathogenic Bacteria and Fungi},
journal = {American Journal of Clinical and Experimental Medicine},
volume = {3},
number = {3},
pages = {118-123},
doi = {10.11648/j.ajcem.20150303.18},
url = {https://doi.org/10.11648/j.ajcem.20150303.18},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150303.18},
abstract = {Background: Lippia adoensis var. koseret is a well known medicinal plant endemic to Ethiopia. It has been traditionally used to treat different infectious diseases and also in food preparation as condiment. The aim of the current study was to evaluate antibacterial and antifungal activities of water, ethanol and methanol based crude extracts of L. adoensis var. koseret against selected human pathogenic bacteria and fungi. Methods: Crude extracts of L. adoensis var. koseret were extracted by maceration method. Disc diffusion assay of the extracts were carried out in four different concentrations against three different bacteria species (Staphylococcus aureus, Enterococcus faecalis and Escherichia coli) and two clinical isolated fungal species (Candida albicans and Aspergillus flavus) by using Kirby- Baur disk diffusion method. Agar dilution method was used to determine the minimum inhibitory concentration, the minimum bactericidal and fungicidal concentrations of the extracts against similar microorganisms. Results: Water-based extract of L. adoensis var. koseret exhibited significantly less antimicrobial activity as compared to ethanol and methanol crude extract against tested isolates of bacteria and fungi (P < 0.05); while, there was no significant difference between ethanol and methanol extracts. Among the tested microorganism S. aureus, was the most sensitive of all whereas C. albicans was the most resistant microorganism to alcohol based extract of L. adoensis var. koseret. The minimum inhibitory concentration of L. adoensis var. koseret ranged from 3.12 to 25mg/ml in the alcohol based extracts but it was higher in the water-based extract. The lower bactericidal concentration (5.20 mg/l) and the highest fungicidal concentration (37.50 mg/ml) were observed in methanol based extracts against S. aureus and C. albicans, respectively. Conclusions: Antimicrobial activity of L. adoensis var. koseret varies with extraction solvent and tested microorganisms.},
year = {2015}
}
TY - JOUR T1 - Antimicrobial Activity of Lippia adoensis var. koseret Against Human Pathogenic Bacteria and Fungi AU - Gemechu Ameya Buli AU - Abdella Gure Duga AU - Engda Dessalegn Y1 - 2015/05/19 PY - 2015 N1 - https://doi.org/10.11648/j.ajcem.20150303.18 DO - 10.11648/j.ajcem.20150303.18 T2 - American Journal of Clinical and Experimental Medicine JF - American Journal of Clinical and Experimental Medicine JO - American Journal of Clinical and Experimental Medicine SP - 118 EP - 123 PB - Science Publishing Group SN - 2330-8133 UR - https://doi.org/10.11648/j.ajcem.20150303.18 AB - Background: Lippia adoensis var. koseret is a well known medicinal plant endemic to Ethiopia. It has been traditionally used to treat different infectious diseases and also in food preparation as condiment. The aim of the current study was to evaluate antibacterial and antifungal activities of water, ethanol and methanol based crude extracts of L. adoensis var. koseret against selected human pathogenic bacteria and fungi. Methods: Crude extracts of L. adoensis var. koseret were extracted by maceration method. Disc diffusion assay of the extracts were carried out in four different concentrations against three different bacteria species (Staphylococcus aureus, Enterococcus faecalis and Escherichia coli) and two clinical isolated fungal species (Candida albicans and Aspergillus flavus) by using Kirby- Baur disk diffusion method. Agar dilution method was used to determine the minimum inhibitory concentration, the minimum bactericidal and fungicidal concentrations of the extracts against similar microorganisms. Results: Water-based extract of L. adoensis var. koseret exhibited significantly less antimicrobial activity as compared to ethanol and methanol crude extract against tested isolates of bacteria and fungi (P < 0.05); while, there was no significant difference between ethanol and methanol extracts. Among the tested microorganism S. aureus, was the most sensitive of all whereas C. albicans was the most resistant microorganism to alcohol based extract of L. adoensis var. koseret. The minimum inhibitory concentration of L. adoensis var. koseret ranged from 3.12 to 25mg/ml in the alcohol based extracts but it was higher in the water-based extract. The lower bactericidal concentration (5.20 mg/l) and the highest fungicidal concentration (37.50 mg/ml) were observed in methanol based extracts against S. aureus and C. albicans, respectively. Conclusions: Antimicrobial activity of L. adoensis var. koseret varies with extraction solvent and tested microorganisms. VL - 3 IS - 3 ER -
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