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On Chip Detection of Zika Virus Based on Loop Mediated Isothermal Amplification

Received: 22 October 2021     Accepted: 19 November 2021     Published: 24 November 2021
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Abstract

There was a world-wide Zika virus (ZIKV) epidemic occurred in 2015, with the major concern of about 20-fold increase in fetuse microcephaly rate in Brazil. To improve the ZIKV point-of-care (POC) molecular diagnostic, we established a rapid and sensitive real-time fluorescence quantitative loop mediated isothermal amplification (LAMP) method and further applied it on a self-fabricated microfluidic chip. After optimization of LAMP reaction conditions, the assay achieved the detection limit of single copy of the standard plasmid in a reaction. Linear regression analysis revealed that the correlation coefficients (R2) were 0.9931. No cross reaction was observed in the controls of yellow fever clinical specimen and several known human influenza viruses, including seasonal A/H1N1, A/H7N9, A/H9N2 and B. To evaluate the performance characteristics of the ZIKV-LAMP assay, we detected 39 clinical specimens by both LAMP assay and real-time RT-PCR, which obtained with completely consistent results. The sensitivity, specificity and performance characteristics of the ZIKV-LAMP assay conformed its utility in ZIKV determination. Moreover, we had also developed a real-time fluorescence detection biomedical system with microfluidic chips. The microfluidic chips were designed with four microcells and the volume of the LAMP reaction was greatly reduced from about 25μL to 10μL. Our newly established real-time fluorescence LAMP detection system with microfluidic chips has the potential for ZIKV POC diagnostics with the advantages of low cost, short analytical time, disposability, low reagent and sample consumption and so on.

Published in American Journal of Biomedical and Life Sciences (Volume 9, Issue 6)
DOI 10.11648/j.ajbls.20210906.16
Page(s) 302-306
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

ZikaVirus, Fetal Microcephaly, Loop Mediated Isothermal Amplification Method, Microfluidic Chip

References
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Cite This Article
  • APA Style

    Hong Yin, Xing Chen, Yong Jin, Bo Liu, Rongmeng Jiang. (2021). On Chip Detection of Zika Virus Based on Loop Mediated Isothermal Amplification. American Journal of Biomedical and Life Sciences, 9(6), 302-306. https://doi.org/10.11648/j.ajbls.20210906.16

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    ACS Style

    Hong Yin; Xing Chen; Yong Jin; Bo Liu; Rongmeng Jiang. On Chip Detection of Zika Virus Based on Loop Mediated Isothermal Amplification. Am. J. Biomed. Life Sci. 2021, 9(6), 302-306. doi: 10.11648/j.ajbls.20210906.16

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    AMA Style

    Hong Yin, Xing Chen, Yong Jin, Bo Liu, Rongmeng Jiang. On Chip Detection of Zika Virus Based on Loop Mediated Isothermal Amplification. Am J Biomed Life Sci. 2021;9(6):302-306. doi: 10.11648/j.ajbls.20210906.16

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  • @article{10.11648/j.ajbls.20210906.16,
      author = {Hong Yin and Xing Chen and Yong Jin and Bo Liu and Rongmeng Jiang},
      title = {On Chip Detection of Zika Virus Based on Loop Mediated Isothermal Amplification},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {9},
      number = {6},
      pages = {302-306},
      doi = {10.11648/j.ajbls.20210906.16},
      url = {https://doi.org/10.11648/j.ajbls.20210906.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20210906.16},
      abstract = {There was a world-wide Zika virus (ZIKV) epidemic occurred in 2015, with the major concern of about 20-fold increase in fetuse microcephaly rate in Brazil. To improve the ZIKV point-of-care (POC) molecular diagnostic, we established a rapid and sensitive real-time fluorescence quantitative loop mediated isothermal amplification (LAMP) method and further applied it on a self-fabricated microfluidic chip. After optimization of LAMP reaction conditions, the assay achieved the detection limit of single copy of the standard plasmid in a reaction. Linear regression analysis revealed that the correlation coefficients (R2) were 0.9931. No cross reaction was observed in the controls of yellow fever clinical specimen and several known human influenza viruses, including seasonal A/H1N1, A/H7N9, A/H9N2 and B. To evaluate the performance characteristics of the ZIKV-LAMP assay, we detected 39 clinical specimens by both LAMP assay and real-time RT-PCR, which obtained with completely consistent results. The sensitivity, specificity and performance characteristics of the ZIKV-LAMP assay conformed its utility in ZIKV determination. Moreover, we had also developed a real-time fluorescence detection biomedical system with microfluidic chips. The microfluidic chips were designed with four microcells and the volume of the LAMP reaction was greatly reduced from about 25μL to 10μL. Our newly established real-time fluorescence LAMP detection system with microfluidic chips has the potential for ZIKV POC diagnostics with the advantages of low cost, short analytical time, disposability, low reagent and sample consumption and so on.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - On Chip Detection of Zika Virus Based on Loop Mediated Isothermal Amplification
    AU  - Hong Yin
    AU  - Xing Chen
    AU  - Yong Jin
    AU  - Bo Liu
    AU  - Rongmeng Jiang
    Y1  - 2021/11/24
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ajbls.20210906.16
    DO  - 10.11648/j.ajbls.20210906.16
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 302
    EP  - 306
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20210906.16
    AB  - There was a world-wide Zika virus (ZIKV) epidemic occurred in 2015, with the major concern of about 20-fold increase in fetuse microcephaly rate in Brazil. To improve the ZIKV point-of-care (POC) molecular diagnostic, we established a rapid and sensitive real-time fluorescence quantitative loop mediated isothermal amplification (LAMP) method and further applied it on a self-fabricated microfluidic chip. After optimization of LAMP reaction conditions, the assay achieved the detection limit of single copy of the standard plasmid in a reaction. Linear regression analysis revealed that the correlation coefficients (R2) were 0.9931. No cross reaction was observed in the controls of yellow fever clinical specimen and several known human influenza viruses, including seasonal A/H1N1, A/H7N9, A/H9N2 and B. To evaluate the performance characteristics of the ZIKV-LAMP assay, we detected 39 clinical specimens by both LAMP assay and real-time RT-PCR, which obtained with completely consistent results. The sensitivity, specificity and performance characteristics of the ZIKV-LAMP assay conformed its utility in ZIKV determination. Moreover, we had also developed a real-time fluorescence detection biomedical system with microfluidic chips. The microfluidic chips were designed with four microcells and the volume of the LAMP reaction was greatly reduced from about 25μL to 10μL. Our newly established real-time fluorescence LAMP detection system with microfluidic chips has the potential for ZIKV POC diagnostics with the advantages of low cost, short analytical time, disposability, low reagent and sample consumption and so on.
    VL  - 9
    IS  - 6
    ER  - 

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Author Information
  • Institute of Equipment and Technology, Chinese Academy of Inspection and Quarantine, Beijing, China

  • State Key Laboratory of Transducer Tech., Institute of Electronics, Chinese Academy of Sciences, Beijing, China

  • Institute of Equipment and Technology, Chinese Academy of Inspection and Quarantine, Beijing, China

  • Institute of Equipment and Technology, Chinese Academy of Inspection and Quarantine, Beijing, China

  • Clinical and Research Center of Infectious Diseases, Beijing Ditan Hospital Capital Medical University, Beijing, China

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