A protein fraction isolated from green algae Halimeda macrobola taken from the sea of Selayar and Kapoposang Island in South Sulawesi was tested for antioxidant and anticancer properties. The protein was isolated using buffer Tris (hydroxymethyl) amino methane. Initial purification of protein was conducted by using the fractionation method with ammonium sulphate, followed by dialysis process. Protein concentration was determined by Lowry method. Antioxidant assay was done by using DPPH method and anticancer activity test by Brine Shrimp Lethality Test (BSLT) method. Anticancer activity was further confirmed by antimitotic test using urchin zygote cells. The results showed that the protein concentration of the crude extract was 0.920 mg/mL. The highest concentration of protein fractions was indicated by the fraction 40-60%, with 1.015 mg/mL. The strong antioxidant activity was shown in the protein fraction of 0-20% saturation with IC50 values of 0.110 mg/mL.The highest activity in the anticancer tests was shown in fractions 0-20% saturation with LC50 values of 0.29 µg/mL and IC50 value of 53.80 µg/mL. The protein fraction 0-20% saturation had a potential to be developed as antioxidant and anticancer agent. These results demonstrate that inexpensive green algae Halimeda macrobola could be a new alternative to produce antioxidant and anticancer proteins.
| Published in | American Journal of Biomedical and Life Sciences (Volume 2, Issue 5) |
| DOI | 10.11648/j.ajbls.20140205.15 |
| Page(s) | 134-140 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Green Algae, Protein Fraction, Toxicity, Antioxidant, Anticancer
| [1] | A. Nontji, 2002, Archipelago Sea, Djambatan Publisher, Jakarta, pp. 4-6 and 145-147, |
| [2] | R. Rachmat, 1999, Natural Products Utilization of Marine Algae for Drugs and Cosmetics, Paper presented at the VII Forum Communications Procedings Pra Kipnas I Physiology Association of Indonesia (IFI), Indonesian Institute of Sciences (LIPI), Serpong, 08 September 1999, |
| [3] | World Health Organization, 2012, Cancer (online), http://www.who.int/mediacentre/factsheets/fs297/index.html, acces on October 05, 2012. |
| [4] | I.C., Sheih, T.J., Fang Wu, T.K., Lin, 2010, Anticancer and antioxidant activities of the peptide fraction from algae protein waste. J Agric Food Chem. 58(2), pp.1202-1207. |
| [5] | T. Sugahara, Y. Ohama, A. Fukuda, et al. 2001, The cytotoxic effect of Eucheuma serra agglutinin (ESA) on cancer cells and its application to molecular probe for drug delivery system using lipid vesicles, Cytotechnology. 36(1-3), pp. 93–99. |
| [6] | S. Dali, H. Natsir, H. Usman, and A. Ahmad, 2011, Antibacterial Bioactivity Protein Fraction of Red Algae Gelidium amansii from Takalar Cikoang Aquatic, South Sulawesi, Pharmacy Magazine and Pharmacology, 15 (1): pp. 47-52. |
| [7] | M. Hayes, (Ed). 2012, Marine Bioactive Compounds: Sources, Characterization and Applications. Springer-Science, New York, London. |
| [8] | H.C., Schro¨der, H., Ushijima, A., Krasko, V., Gamulin, N.L., Thakur, B., Diehl-Seifert, I.M., Muller, and W.E.G., Muller, 2003, Emergence and Disappearance of an immune molecule, an antimicrobial lectin, in basal metazoan. A TACHYLECTIN-RELATED PROTEIN IN THE SPONGE SUBERITES DOMUNCULA. J. Biol. Chem., 278, pp.32810-32817. |
| [9] | O. H., Lowry, N. J., Rosebrough, A. L., Farr, and R. J., Randall, 1951, Protein Measurement with The Folin Phenol Reagent, J. Biol. Chem., 193: pp.265-275. |
| [10] | Juniarti, D. Osmeli, and Yuhernita, 2009, Content of Chemical Compounds, Toxicity Test (Brine Shrimp Lethality Test) and Antioxidants (1,1-diphenyl-2-picrilhydrazil) from the Leaf Extract Saga (Abrus precatorius L.), Makara Sains, 13 (1): pp. 50-55. |
| [11] | P. Molyneux, 2004, The use of stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity, Songklanakarin J Sci Technol., 26 (2): pp. 211-219. |
| [12] | N., Weerapreyakul, A., Nonpunya, S., Barusrux, T. Thitimetharoc, and B. Sripanidkulchai, 2012, Evaluation of the Anticancer Potential of Six Herbs against a Hepatoma Cell Line, Chinese Medicine, 7 (15): pp. 2-7. |
| [13] | A. P. D., Nurhayati, N. Abdulgani, and R., Febrianto, 2006, Toxicity Test of Eucheuma alvarezii Extract against Artemia salina as Potential Anticancer Preliminary Study, Indonesia Chemical Deed (Kimindo Deed), 2 (1): pp.41-46. |
| [14] | S. D., Marliyana, F. W., Rakhman, H., Nestri, and R., Rakhmawati, 2012, Toxicity Test (Brine Shrimp Lethality Test) of Whole Extract from Pandanus conoideus L., Alchemy Jurnal Penelitian Kimia, 8 (1): pp. 24-33. |
| [15] | B. N., Meyer, N. R., Ferrigni, J. E., Putman, L. B., Jacbsen, D. E., Nicols, and J. L. McLaughlin, 1982, Brine Shrimp : A Comvenient general Bioassay For Active Plant Constituents. Plant Medica. Pp.31-34. |
| [16] | E., Johannes, Syafaraenan, R., Agus, dan R., Umar, 2013, Antimitotic activity of β-sitosterol Isolates from Hydroid Aglaophenia Cupressina Lamoureoux the Early Cleavage Cell Zygote of Urchin Tripneus tesgratilla Linn., MANASIR, 1 (1): pp. 27-32. |
APA Style
Ahyar Ahmad, Hanapi Usman, Hasnah Natsir, Abdul Karim. (2014). Isolation and Characterization of Bioactive Protein from Green Algae Halimeda macrobola Acting as Antioxidant and Anticancer Agent. American Journal of Biomedical and Life Sciences, 2(5), 134-140. https://doi.org/10.11648/j.ajbls.20140205.15
ACS Style
Ahyar Ahmad; Hanapi Usman; Hasnah Natsir; Abdul Karim. Isolation and Characterization of Bioactive Protein from Green Algae Halimeda macrobola Acting as Antioxidant and Anticancer Agent. Am. J. Biomed. Life Sci. 2014, 2(5), 134-140. doi: 10.11648/j.ajbls.20140205.15
AMA Style
Ahyar Ahmad, Hanapi Usman, Hasnah Natsir, Abdul Karim. Isolation and Characterization of Bioactive Protein from Green Algae Halimeda macrobola Acting as Antioxidant and Anticancer Agent. Am J Biomed Life Sci. 2014;2(5):134-140. doi: 10.11648/j.ajbls.20140205.15
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ajbls.20140205.15,
author = {Ahyar Ahmad and Hanapi Usman and Hasnah Natsir and Abdul Karim},
title = {Isolation and Characterization of Bioactive Protein from Green Algae Halimeda macrobola Acting as Antioxidant and Anticancer Agent},
journal = {American Journal of Biomedical and Life Sciences},
volume = {2},
number = {5},
pages = {134-140},
doi = {10.11648/j.ajbls.20140205.15},
url = {https://doi.org/10.11648/j.ajbls.20140205.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140205.15},
abstract = {A protein fraction isolated from green algae Halimeda macrobola taken from the sea of Selayar and Kapoposang Island in South Sulawesi was tested for antioxidant and anticancer properties. The protein was isolated using buffer Tris (hydroxymethyl) amino methane. Initial purification of protein was conducted by using the fractionation method with ammonium sulphate, followed by dialysis process. Protein concentration was determined by Lowry method. Antioxidant assay was done by using DPPH method and anticancer activity test by Brine Shrimp Lethality Test (BSLT) method. Anticancer activity was further confirmed by antimitotic test using urchin zygote cells. The results showed that the protein concentration of the crude extract was 0.920 mg/mL. The highest concentration of protein fractions was indicated by the fraction 40-60%, with 1.015 mg/mL. The strong antioxidant activity was shown in the protein fraction of 0-20% saturation with IC50 values of 0.110 mg/mL.The highest activity in the anticancer tests was shown in fractions 0-20% saturation with LC50 values of 0.29 µg/mL and IC50 value of 53.80 µg/mL. The protein fraction 0-20% saturation had a potential to be developed as antioxidant and anticancer agent. These results demonstrate that inexpensive green algae Halimeda macrobola could be a new alternative to produce antioxidant and anticancer proteins.},
year = {2014}
}
TY - JOUR T1 - Isolation and Characterization of Bioactive Protein from Green Algae Halimeda macrobola Acting as Antioxidant and Anticancer Agent AU - Ahyar Ahmad AU - Hanapi Usman AU - Hasnah Natsir AU - Abdul Karim Y1 - 2014/10/30 PY - 2014 N1 - https://doi.org/10.11648/j.ajbls.20140205.15 DO - 10.11648/j.ajbls.20140205.15 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 134 EP - 140 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20140205.15 AB - A protein fraction isolated from green algae Halimeda macrobola taken from the sea of Selayar and Kapoposang Island in South Sulawesi was tested for antioxidant and anticancer properties. The protein was isolated using buffer Tris (hydroxymethyl) amino methane. Initial purification of protein was conducted by using the fractionation method with ammonium sulphate, followed by dialysis process. Protein concentration was determined by Lowry method. Antioxidant assay was done by using DPPH method and anticancer activity test by Brine Shrimp Lethality Test (BSLT) method. Anticancer activity was further confirmed by antimitotic test using urchin zygote cells. The results showed that the protein concentration of the crude extract was 0.920 mg/mL. The highest concentration of protein fractions was indicated by the fraction 40-60%, with 1.015 mg/mL. The strong antioxidant activity was shown in the protein fraction of 0-20% saturation with IC50 values of 0.110 mg/mL.The highest activity in the anticancer tests was shown in fractions 0-20% saturation with LC50 values of 0.29 µg/mL and IC50 value of 53.80 µg/mL. The protein fraction 0-20% saturation had a potential to be developed as antioxidant and anticancer agent. These results demonstrate that inexpensive green algae Halimeda macrobola could be a new alternative to produce antioxidant and anticancer proteins. VL - 2 IS - 5 ER -
Information
Email: