Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali

Sow Ibrahim; Dakouo Marthin; Coulibaly Kadidiatou; Diakité Mohamed Adama; Koné Yaya Sidi; Diakité Adama; Sidibé Satigui; Babana Amadou Hamadoun

doi:doi:10.11648/j.ajbes.20220801.11

Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali

Sow Ibrahim, Dakouo Marthin, Coulibaly Kadidiatou, Diakité Mohamed Adama, Koné Yaya Sidi, Diakité Adama, Sidibé Satigui, Babana Amadou Hamadoun

Published in American Journal of Biological and Environmental Statistics (Volume 8, Issue 1)

Received: 21 December 2021 Accepted: 10 January 2022 Published: 20 January 2022

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Abstract

Rabies are one of the major zoonoses in Mali. The control of this disease is based on the use of effective diagnostic techniques, the management of human cases after exposure and medical prophylaxis of animals. Diagnosis is usually made by detection of viral antigen by direct immunofluorescence (IF). Due to the increasing variability of the genus Lyssavirus observed, it was found necessary to use a complementary technique, namely reverse transcription in polymerase chain reaction (RT-PCR). The objective of the present study was to assess the diagnostic performance of IFD and RT-PCR for the diagnosis of animal rabies in Mali with a view to characterizing the different strains identified at the molecular level (gene of rabies virus nucleoprotein N). Thus, the analysis of forty (40) brain samples from dogs suspected of having rabies, revealed 36 positive cases for IFD, i.e. 90% and 33 for RT-PCR, i.e. 82.5.% and a concordance rate of 82.5%. Analysis of the segment of the gene of nucleoprotein (N) showed that the rabies viruses circulating in Mali belong to the Africa 2 lineage (Africa 2). Statistical analyzes showed the existence of a significant difference (P=0.001<0.005) between the performance of the two techniques. The present study shows the usefulness of the complementarity of the two tests as an alternative for confirming the diagnosis of rabies.

Published in	American Journal of Biological and Environmental Statistics (Volume 8, Issue 1)
DOI	10.11648/j.ajbes.20220801.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Canine Rabies, Comparative Diagnosis, IFD, RT-PCR, Genotype

References

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APA Style

Sow Ibrahim, Dakouo Marthin, Coulibaly Kadidiatou, Diakité Mohamed Adama, Koné Yaya Sidi, et al. (2022). Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali. American Journal of Biological and Environmental Statistics, 8(1), 1-7. https://doi.org/10.11648/j.ajbes.20220801.11

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ACS Style

Sow Ibrahim; Dakouo Marthin; Coulibaly Kadidiatou; Diakité Mohamed Adama; Koné Yaya Sidi, et al. Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali. Am. J. Biol. Environ. Stat. 2022, 8(1), 1-7. doi: 10.11648/j.ajbes.20220801.11

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AMA Style

Sow Ibrahim, Dakouo Marthin, Coulibaly Kadidiatou, Diakité Mohamed Adama, Koné Yaya Sidi, et al. Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali. Am J Biol Environ Stat. 2022;8(1):1-7. doi: 10.11648/j.ajbes.20220801.11

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@article{10.11648/j.ajbes.20220801.11,
  author = {Sow Ibrahim and Dakouo Marthin and Coulibaly Kadidiatou and Diakité Mohamed Adama and Koné Yaya Sidi and Diakité Adama and Sidibé Satigui and Babana Amadou Hamadoun},
  title = {Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali},
  journal = {American Journal of Biological and Environmental Statistics},
  volume = {8},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ajbes.20220801.11},
  url = {https://doi.org/10.11648/j.ajbes.20220801.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbes.20220801.11},
  abstract = {Rabies are one of the major zoonoses in Mali. The control of this disease is based on the use of effective diagnostic techniques, the management of human cases after exposure and medical prophylaxis of animals. Diagnosis is usually made by detection of viral antigen by direct immunofluorescence (IF). Due to the increasing variability of the genus Lyssavirus observed, it was found necessary to use a complementary technique, namely reverse transcription in polymerase chain reaction (RT-PCR). The objective of the present study was to assess the diagnostic performance of IFD and RT-PCR for the diagnosis of animal rabies in Mali with a view to characterizing the different strains identified at the molecular level (gene of rabies virus nucleoprotein N). Thus, the analysis of forty (40) brain samples from dogs suspected of having rabies, revealed 36 positive cases for IFD, i.e. 90% and 33 for RT-PCR, i.e. 82.5.% and a concordance rate of 82.5%. Analysis of the segment of the gene of nucleoprotein (N) showed that the rabies viruses circulating in Mali belong to the Africa 2 lineage (Africa 2). Statistical analyzes showed the existence of a significant difference (P=0.001<0.005) between the performance of the two techniques. The present study shows the usefulness of the complementarity of the two tests as an alternative for confirming the diagnosis of rabies.},
 year = {2022}
}

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TY - JOUR
T1 - Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali
AU - Sow Ibrahim
AU - Dakouo Marthin
AU - Coulibaly Kadidiatou
AU - Diakité Mohamed Adama
AU - Koné Yaya Sidi
AU - Diakité Adama
AU - Sidibé Satigui
AU - Babana Amadou Hamadoun
Y1 - 2022/01/20
PY - 2022
N1 - https://doi.org/10.11648/j.ajbes.20220801.11
DO - 10.11648/j.ajbes.20220801.11
T2 - American Journal of Biological and Environmental Statistics
JF - American Journal of Biological and Environmental Statistics
JO - American Journal of Biological and Environmental Statistics
SP - 1
EP - 7
PB - Science Publishing Group
SN - 2471-979X
UR - https://doi.org/10.11648/j.ajbes.20220801.11
AB - Rabies are one of the major zoonoses in Mali. The control of this disease is based on the use of effective diagnostic techniques, the management of human cases after exposure and medical prophylaxis of animals. Diagnosis is usually made by detection of viral antigen by direct immunofluorescence (IF). Due to the increasing variability of the genus Lyssavirus observed, it was found necessary to use a complementary technique, namely reverse transcription in polymerase chain reaction (RT-PCR). The objective of the present study was to assess the diagnostic performance of IFD and RT-PCR for the diagnosis of animal rabies in Mali with a view to characterizing the different strains identified at the molecular level (gene of rabies virus nucleoprotein N). Thus, the analysis of forty (40) brain samples from dogs suspected of having rabies, revealed 36 positive cases for IFD, i.e. 90% and 33 for RT-PCR, i.e. 82.5.% and a concordance rate of 82.5%. Analysis of the segment of the gene of nucleoprotein (N) showed that the rabies viruses circulating in Mali belong to the Africa 2 lineage (Africa 2). Statistical analyzes showed the existence of a significant difference (P=0.001<0.005) between the performance of the two techniques. The present study shows the usefulness of the complementarity of the two tests as an alternative for confirming the diagnosis of rabies.
VL - 8
IS - 1
ER -

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Author Information

Sow Ibrahim

Departement of Biology, Faculty of Sciences & Techniques, University of Sciences, Bamako, Mali
Dakouo Marthin

Diagnosis and Research Service, Central Veterinary Laboratory, Bamako, Mali
Coulibaly Kadidiatou

Diagnosis and Research Service, Central Veterinary Laboratory, Bamako, Mali
Diakité Mohamed Adama

Diagnosis and Research Service, Central Veterinary Laboratory, Bamako, Mali
Koné Yaya Sidi

Diagnosis and Research Service, Central Veterinary Laboratory, Bamako, Mali
Diakité Adama

Diagnosis and Research Service, Central Veterinary Laboratory, Bamako, Mali
Sidibé Satigui

Diagnosis and Research Service, Central Veterinary Laboratory, Bamako, Mali
Babana Amadou Hamadoun

Departement of Biology, Faculty of Sciences & Techniques, University of Sciences, Bamako, Mali

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APA Style

Sow Ibrahim, Dakouo Marthin, Coulibaly Kadidiatou, Diakité Mohamed Adama, Koné Yaya Sidi, et al. (2022). Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali. American Journal of Biological and Environmental Statistics, 8(1), 1-7. https://doi.org/10.11648/j.ajbes.20220801.11

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ACS Style

Sow Ibrahim; Dakouo Marthin; Coulibaly Kadidiatou; Diakité Mohamed Adama; Koné Yaya Sidi, et al. Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali. Am. J. Biol. Environ. Stat. 2022, 8(1), 1-7. doi: 10.11648/j.ajbes.20220801.11

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AMA Style

Sow Ibrahim, Dakouo Marthin, Coulibaly Kadidiatou, Diakité Mohamed Adama, Koné Yaya Sidi, et al. Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali. Am J Biol Environ Stat. 2022;8(1):1-7. doi: 10.11648/j.ajbes.20220801.11

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@article{10.11648/j.ajbes.20220801.11,
  author = {Sow Ibrahim and Dakouo Marthin and Coulibaly Kadidiatou and Diakité Mohamed Adama and Koné Yaya Sidi and Diakité Adama and Sidibé Satigui and Babana Amadou Hamadoun},
  title = {Molecular Characterization of Canine Rabies Virus Strains Circulating in Mali},
  journal = {American Journal of Biological and Environmental Statistics},
  volume = {8},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ajbes.20220801.11},
  url = {https://doi.org/10.11648/j.ajbes.20220801.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbes.20220801.11},
  abstract = {Rabies are one of the major zoonoses in Mali. The control of this disease is based on the use of effective diagnostic techniques, the management of human cases after exposure and medical prophylaxis of animals. Diagnosis is usually made by detection of viral antigen by direct immunofluorescence (IF). Due to the increasing variability of the genus Lyssavirus observed, it was found necessary to use a complementary technique, namely reverse transcription in polymerase chain reaction (RT-PCR). The objective of the present study was to assess the diagnostic performance of IFD and RT-PCR for the diagnosis of animal rabies in Mali with a view to characterizing the different strains identified at the molecular level (gene of rabies virus nucleoprotein N). Thus, the analysis of forty (40) brain samples from dogs suspected of having rabies, revealed 36 positive cases for IFD, i.e. 90% and 33 for RT-PCR, i.e. 82.5.% and a concordance rate of 82.5%. Analysis of the segment of the gene of nucleoprotein (N) showed that the rabies viruses circulating in Mali belong to the Africa 2 lineage (Africa 2). Statistical analyzes showed the existence of a significant difference (P=0.001<0.005) between the performance of the two techniques. The present study shows the usefulness of the complementarity of the two tests as an alternative for confirming the diagnosis of rabies.},
 year = {2022}
}

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