Diarrhoeal infection is defined as a significant reason of morbidity as well as mortality all over the world. Isolation of Shigella, the most significant etiological agents of diarrhea in developing countries, was missing occasionally when patients feces are cultured by conventional procedures. The collected 200 samples from hospitalized diarrhoeal patients in Tangail district, Bangladesh during 2010-2011, Shigella were isolated from 24 (12%) samples utilizing conventional culture methods. Among176 culture negative stool samples,ipa H gene, specific marker to identify Shigella species was detected from 14 (8%) by PCR. Furthermore, these samples which possess the ipa H gene were cultured and identified as Shigella-like organism (s) by utilizing various phenotypic as well as molecular tools. Of 24 Shigella spp. which was identified using conventional culture methods, S. flexneri were dominant 41.7%, followed by S. boydii 29.2%, S. sonnei 25%, and S. dysenteriae 4.2%. Among 14 of 176 culture negative ipaH positive samples, 7 were identified as Shigella sspp., of which, 3 were S. flexneri, 1 was S. boydii, 2 were S. sonnei and 1 identified as a novel group of S. dysenteriae designated as KIVI 156. Additionally, all Shigella strains were resistant to ampicillin, sulfamethaxol-trimethoprime and were susceptible to ciprofloxacin and mecillinam. This study demonstrated that the conventional culture method cannot be referred as an adequate method to focus the actual disease burden caused by Shigella.
| Published in | American Journal of Biological and Environmental Statistics (Volume 7, Issue 2) |
| DOI | 10.11648/j.ajbes.20210702.11 |
| Page(s) | 39-43 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2021. Published by Science Publishing Group |
Diarrhoea, Shigellosis, Polymerase Chain Reaction (PCR)
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APA Style
Tarana Sharmin, Mahmuda Akter, Atanu Banik, Ahmed Faisal Sumit, Tasnim Ahmed, et al. (2021). Molecular Approaches for Isolation of Shigella Species from Culture Negative Diarrheal Samples: A Hospital Based Study in Bangladesh. American Journal of Biological and Environmental Statistics, 7(2), 39-43. https://doi.org/10.11648/j.ajbes.20210702.11
ACS Style
Tarana Sharmin; Mahmuda Akter; Atanu Banik; Ahmed Faisal Sumit; Tasnim Ahmed, et al. Molecular Approaches for Isolation of Shigella Species from Culture Negative Diarrheal Samples: A Hospital Based Study in Bangladesh. Am. J. Biol. Environ. Stat. 2021, 7(2), 39-43. doi: 10.11648/j.ajbes.20210702.11
AMA Style
Tarana Sharmin, Mahmuda Akter, Atanu Banik, Ahmed Faisal Sumit, Tasnim Ahmed, et al. Molecular Approaches for Isolation of Shigella Species from Culture Negative Diarrheal Samples: A Hospital Based Study in Bangladesh. Am J Biol Environ Stat. 2021;7(2):39-43. doi: 10.11648/j.ajbes.20210702.11
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Download@article{10.11648/j.ajbes.20210702.11,
author = {Tarana Sharmin and Mahmuda Akter and Atanu Banik and Ahmed Faisal Sumit and Tasnim Ahmed and Shahin Mahmud and Ashraf Hossain Talukder and Kaisar Ali Talukder},
title = {Molecular Approaches for Isolation of Shigella Species from Culture Negative Diarrheal Samples: A Hospital Based Study in Bangladesh},
journal = {American Journal of Biological and Environmental Statistics},
volume = {7},
number = {2},
pages = {39-43},
doi = {10.11648/j.ajbes.20210702.11},
url = {https://doi.org/10.11648/j.ajbes.20210702.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbes.20210702.11},
abstract = {Diarrhoeal infection is defined as a significant reason of morbidity as well as mortality all over the world. Isolation of Shigella, the most significant etiological agents of diarrhea in developing countries, was missing occasionally when patients feces are cultured by conventional procedures. The collected 200 samples from hospitalized diarrhoeal patients in Tangail district, Bangladesh during 2010-2011, Shigella were isolated from 24 (12%) samples utilizing conventional culture methods. Among176 culture negative stool samples,ipa H gene, specific marker to identify Shigella species was detected from 14 (8%) by PCR. Furthermore, these samples which possess the ipa H gene were cultured and identified as Shigella-like organism (s) by utilizing various phenotypic as well as molecular tools. Of 24 Shigella spp. which was identified using conventional culture methods, S. flexneri were dominant 41.7%, followed by S. boydii 29.2%, S. sonnei 25%, and S. dysenteriae 4.2%. Among 14 of 176 culture negative ipaH positive samples, 7 were identified as Shigella sspp., of which, 3 were S. flexneri, 1 was S. boydii, 2 were S. sonnei and 1 identified as a novel group of S. dysenteriae designated as KIVI 156. Additionally, all Shigella strains were resistant to ampicillin, sulfamethaxol-trimethoprime and were susceptible to ciprofloxacin and mecillinam. This study demonstrated that the conventional culture method cannot be referred as an adequate method to focus the actual disease burden caused by Shigella.},
year = {2021}
}
TY - JOUR T1 - Molecular Approaches for Isolation of Shigella Species from Culture Negative Diarrheal Samples: A Hospital Based Study in Bangladesh AU - Tarana Sharmin AU - Mahmuda Akter AU - Atanu Banik AU - Ahmed Faisal Sumit AU - Tasnim Ahmed AU - Shahin Mahmud AU - Ashraf Hossain Talukder AU - Kaisar Ali Talukder Y1 - 2021/05/31 PY - 2021 N1 - https://doi.org/10.11648/j.ajbes.20210702.11 DO - 10.11648/j.ajbes.20210702.11 T2 - American Journal of Biological and Environmental Statistics JF - American Journal of Biological and Environmental Statistics JO - American Journal of Biological and Environmental Statistics SP - 39 EP - 43 PB - Science Publishing Group SN - 2471-979X UR - https://doi.org/10.11648/j.ajbes.20210702.11 AB - Diarrhoeal infection is defined as a significant reason of morbidity as well as mortality all over the world. Isolation of Shigella, the most significant etiological agents of diarrhea in developing countries, was missing occasionally when patients feces are cultured by conventional procedures. The collected 200 samples from hospitalized diarrhoeal patients in Tangail district, Bangladesh during 2010-2011, Shigella were isolated from 24 (12%) samples utilizing conventional culture methods. Among176 culture negative stool samples,ipa H gene, specific marker to identify Shigella species was detected from 14 (8%) by PCR. Furthermore, these samples which possess the ipa H gene were cultured and identified as Shigella-like organism (s) by utilizing various phenotypic as well as molecular tools. Of 24 Shigella spp. which was identified using conventional culture methods, S. flexneri were dominant 41.7%, followed by S. boydii 29.2%, S. sonnei 25%, and S. dysenteriae 4.2%. Among 14 of 176 culture negative ipaH positive samples, 7 were identified as Shigella sspp., of which, 3 were S. flexneri, 1 was S. boydii, 2 were S. sonnei and 1 identified as a novel group of S. dysenteriae designated as KIVI 156. Additionally, all Shigella strains were resistant to ampicillin, sulfamethaxol-trimethoprime and were susceptible to ciprofloxacin and mecillinam. This study demonstrated that the conventional culture method cannot be referred as an adequate method to focus the actual disease burden caused by Shigella. VL - 7 IS - 2 ER -
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