Isolation and Characterization of Protease Producing Bacteria from Soil, in Arba Minch University, Abaya Campus

Temam Abrar Hamza

doi:doi:10.11648/j.ajbes.20180401.12

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Isolation and Characterization of Protease Producing Bacteria from Soil, in Arba Minch University, Abaya Campus

Temam Abrar Hamza

Published in American Journal of Biological and Environmental Statistics (Volume 4, Issue 1)

Received: 8 June 2017 Accepted: 23 June 2017 Published: 15 January 2018

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Abstract

Microbes are ubiquitous and live in familiar settings such as soil, water, food and animal intestines as well as in more extreme setting such as rocks, glaciers, hot springs and deep-sea vents. They are essential component of the biodiversity as they account for 50% of the living biomass of this planet. The aim of this work was isolation and screening of protease producing bacteria from soil collected from the study area. Isolation of protease producing bacteria was performed by the serial dilution and spread plate technique using skim milk agar medium. Accordingly, 131 isolates were positive on skim milk agar and of which 13 (10%) of them are protease producer. Four best isolates were selected on the basis of their clear zone size, one from each site for further study. IS-4 has larger clear zone size with 10.5mm followed by IS-2 (6mm). All potential isolates have circular in shape of colony and flat in height of colony except IS-1 which is irregular and raised respectively. Most of potential isolates have opaque in their colonial optical density. All isolates were found to be Gram positive and spore producers. Morphological and biochemical results have revealed that three of four active enzyme producing bacteria are identified as Bacillus spp. Members of the genus Bacillus produce a large variety of extracellular enzymes (proteases) particularly significant for industrial application.

Published in	American Journal of Biological and Environmental Statistics (Volume 4, Issue 1)
DOI	10.11648/j.ajbes.20180401.12
Page(s)	10-14
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Protease, Bacteria, Isolation, Clear Zone

References

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[2]	Fekadu Alemu. Isolation and screening of protease producing bacteria from cheese. International Journal of Nutrition and Food Science. 2015;4:6.
[3]	Temam, A. H.. Bacterial Protease Enzyme: Safe and Good Alternative for Industrial and Commercial Use. International Journal of Chemical and Biomolecular Science. 2017. 3(1): 1-10.
[4]	Kanmani, R., Dhivya, S., Jayalakshmi, S. and Vijayabaskar, P. Studies on detergent additives of protease enzyme from an estuarine bacterium Bacillus cereus. International Research Journal of Biotechnology. 2011;2:7.
[5]	Kumar, D. J. M., Rajan, R., Lawrence, L., priyadarshini, S. and Kalaichelvan, P. T. Destaning and dehairing capability of partially purified Bacillus subtilisprotease from optimized fermentation medium. Asian Journal of Experimental and Biological Science. 2012;3:7.
[6]	Gupta, R.; Beg, Q. K. and Lorenz, P. Bacterial alkaline proteases: molecular approaches and industrial applications. Applied Microbiology and Biotechnology. 2002;59:17.
[7]	Temam, A. H. Isolation and Screening of Protease Producing Bacteria from Local Environment for Detergent Additive, American Journal of Life sciences. 2017. 5(5):116-124.
[8]	Bholay, A. D., More, S. Y., Patil, V. B. and Patil, N. Bacterial extracellular alkaline proteases and its industrial applications. International Research Journal of Biological Sciences. 2012;1:5.
[9]	Temam, A. H. Isolation of Protease Producing Bacteria from Soil for Polyester and Silver Recovery from Waste X-ray Film. American Journal of BioScience. 2017. 5(5):74-79.
[10]	Bergey, D. H. and Holt, J. G. Bergey's manual of determinative bacteriology, 9th edition Williams and Wilkins, Baltimore. 9th edition Williams and Wilkins, Baltimore. 1994.
[11]	Haile, G. and Gessesse A. Properties of alkaline protease C45 produced by alkaliphilic Bacillus Sp. isolated from Chitu, Ethiopian Soda Lake. Journal of Biotechnology and Biomaterial. 2012;2:4.
[12]	Singh, P., Rani, A. and. Chaudhary, N. Isolation and characterization of protease producing Bacillus sp from soil. International Journal of Pharma Sciences and Research. 2015;6:6.
[13]	Najafi, M. F. Potential application of protease isolated from Pseudomonas aeruginosa PD100. Electronic Journal of Biotechnology. 2005;8:6.
[14]	Temam, A. H. and Fantahun, W. Optimization of Culture Growth Parameters for Production of Protease from Bacteria, Isolated from Soil. Bioscience and Bioengineering. 2017. 3(1):1-10.
[15]	Prabhavathy, G., Rajasekara, M., Pandian and Senthilkumar, B. Identification of industrially important alkaline protease producing Bacillus subtilis by 16s rRNA sequence analysis and its applications. International Journal of Research in Pharmaceutical and Biomedical Sciences. 2013;4:6.

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APA Style

Temam Abrar Hamza. (2018). Isolation and Characterization of Protease Producing Bacteria from Soil, in Arba Minch University, Abaya Campus. American Journal of Biological and Environmental Statistics, 4(1), 10-14. https://doi.org/10.11648/j.ajbes.20180401.12

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ACS Style

Temam Abrar Hamza. Isolation and Characterization of Protease Producing Bacteria from Soil, in Arba Minch University, Abaya Campus. Am. J. Biol. Environ. Stat. 2018, 4(1), 10-14. doi: 10.11648/j.ajbes.20180401.12

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AMA Style

Temam Abrar Hamza. Isolation and Characterization of Protease Producing Bacteria from Soil, in Arba Minch University, Abaya Campus. Am J Biol Environ Stat. 2018;4(1):10-14. doi: 10.11648/j.ajbes.20180401.12

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@article{10.11648/j.ajbes.20180401.12,
  author = {Temam Abrar Hamza},
  title = {Isolation and Characterization of Protease Producing Bacteria from Soil, in Arba Minch University, Abaya Campus},
  journal = {American Journal of Biological and Environmental Statistics},
  volume = {4},
  number = {1},
  pages = {10-14},
  doi = {10.11648/j.ajbes.20180401.12},
  url = {https://doi.org/10.11648/j.ajbes.20180401.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbes.20180401.12},
  abstract = {Microbes are ubiquitous and live in familiar settings such as soil, water, food and animal intestines as well as in more extreme setting such as rocks, glaciers, hot springs and deep-sea vents. They are essential component of the biodiversity as they account for 50% of the living biomass of this planet. The aim of this work was isolation and screening of protease producing bacteria from soil collected from the study area. Isolation of protease producing bacteria was performed by the serial dilution and spread plate technique using skim milk agar medium. Accordingly, 131 isolates were positive on skim milk agar and of which 13 (10%) of them are protease producer. Four best isolates were selected on the basis of their clear zone size, one from each site for further study. IS-4 has larger clear zone size with 10.5mm followed by IS-2 (6mm). All potential isolates have circular in shape of colony and flat in height of colony except IS-1 which is irregular and raised respectively. Most of potential isolates have opaque in their colonial optical density. All isolates were found to be Gram positive and spore producers. Morphological and biochemical results have revealed that three of four active enzyme producing bacteria are identified as Bacillus spp. Members of the genus Bacillus produce a large variety of extracellular enzymes (proteases) particularly significant for industrial application.},
 year = {2018}
}

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TY - JOUR
T1 - Isolation and Characterization of Protease Producing Bacteria from Soil, in Arba Minch University, Abaya Campus
AU - Temam Abrar Hamza
Y1 - 2018/01/15
PY - 2018
N1 - https://doi.org/10.11648/j.ajbes.20180401.12
DO - 10.11648/j.ajbes.20180401.12
T2 - American Journal of Biological and Environmental Statistics
JF - American Journal of Biological and Environmental Statistics
JO - American Journal of Biological and Environmental Statistics
SP - 10
EP - 14
PB - Science Publishing Group
SN - 2471-979X
UR - https://doi.org/10.11648/j.ajbes.20180401.12
AB - Microbes are ubiquitous and live in familiar settings such as soil, water, food and animal intestines as well as in more extreme setting such as rocks, glaciers, hot springs and deep-sea vents. They are essential component of the biodiversity as they account for 50% of the living biomass of this planet. The aim of this work was isolation and screening of protease producing bacteria from soil collected from the study area. Isolation of protease producing bacteria was performed by the serial dilution and spread plate technique using skim milk agar medium. Accordingly, 131 isolates were positive on skim milk agar and of which 13 (10%) of them are protease producer. Four best isolates were selected on the basis of their clear zone size, one from each site for further study. IS-4 has larger clear zone size with 10.5mm followed by IS-2 (6mm). All potential isolates have circular in shape of colony and flat in height of colony except IS-1 which is irregular and raised respectively. Most of potential isolates have opaque in their colonial optical density. All isolates were found to be Gram positive and spore producers. Morphological and biochemical results have revealed that three of four active enzyme producing bacteria are identified as Bacillus spp. Members of the genus Bacillus produce a large variety of extracellular enzymes (proteases) particularly significant for industrial application.
VL - 4
IS - 1
ER -

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Author Information

Temam Abrar Hamza

Biotechnology Program, Department of Biology, College of Natural Sciences Arba Minch University, Arba Minch, Ethiopia

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APA Style

Temam Abrar Hamza. (2018). Isolation and Characterization of Protease Producing Bacteria from Soil, in Arba Minch University, Abaya Campus. American Journal of Biological and Environmental Statistics, 4(1), 10-14. https://doi.org/10.11648/j.ajbes.20180401.12

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ACS Style

Temam Abrar Hamza. Isolation and Characterization of Protease Producing Bacteria from Soil, in Arba Minch University, Abaya Campus. Am. J. Biol. Environ. Stat. 2018, 4(1), 10-14. doi: 10.11648/j.ajbes.20180401.12

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AMA Style

Temam Abrar Hamza. Isolation and Characterization of Protease Producing Bacteria from Soil, in Arba Minch University, Abaya Campus. Am J Biol Environ Stat. 2018;4(1):10-14. doi: 10.11648/j.ajbes.20180401.12

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@article{10.11648/j.ajbes.20180401.12,
  author = {Temam Abrar Hamza},
  title = {Isolation and Characterization of Protease Producing Bacteria from Soil, in Arba Minch University, Abaya Campus},
  journal = {American Journal of Biological and Environmental Statistics},
  volume = {4},
  number = {1},
  pages = {10-14},
  doi = {10.11648/j.ajbes.20180401.12},
  url = {https://doi.org/10.11648/j.ajbes.20180401.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbes.20180401.12},
  abstract = {Microbes are ubiquitous and live in familiar settings such as soil, water, food and animal intestines as well as in more extreme setting such as rocks, glaciers, hot springs and deep-sea vents. They are essential component of the biodiversity as they account for 50% of the living biomass of this planet. The aim of this work was isolation and screening of protease producing bacteria from soil collected from the study area. Isolation of protease producing bacteria was performed by the serial dilution and spread plate technique using skim milk agar medium. Accordingly, 131 isolates were positive on skim milk agar and of which 13 (10%) of them are protease producer. Four best isolates were selected on the basis of their clear zone size, one from each site for further study. IS-4 has larger clear zone size with 10.5mm followed by IS-2 (6mm). All potential isolates have circular in shape of colony and flat in height of colony except IS-1 which is irregular and raised respectively. Most of potential isolates have opaque in their colonial optical density. All isolates were found to be Gram positive and spore producers. Morphological and biochemical results have revealed that three of four active enzyme producing bacteria are identified as Bacillus spp. Members of the genus Bacillus produce a large variety of extracellular enzymes (proteases) particularly significant for industrial application.},
 year = {2018}
}

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