Estimation Activity and Partial Purification of Ceruloplasmin from Sera of Patients with Chronic Renal Failure and Healthy Subjects

Israa Ghassan Zainal

doi:doi:10.11648/j.ab.20140206.12

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Estimation Activity and Partial Purification of Ceruloplasmin from Sera of Patients with Chronic Renal Failure and Healthy Subjects

Israa Ghassan Zainal

Published in Advances in Biochemistry (Volume 2, Issue 6)

Received: 15 November 2014 Accepted: 21 November 2014 Published: 29 November 2014

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Abstract

CeruloplasminCP (EC1.16.3.1) activity has been assayed in (32) serum samples of patients with Chronic Renal Failure CRF, and (32) serum samples of healthy individuals without any clinically detectable diseases have been used as control group. The aim of this study is to measure CP activity and partially purifying the enzyme from sera of patients with CRF and healthy individuals. The results of this study revealed that CP activity of patient’s serum shows a highly significant decrease (p < 0.05) compared to that of the healthy individuals. Ceruloplasmin enzyme was partially purified from the serum of patient with CRF and and healthy individual by three steps which operates throughout under mild conditions 4°, acetate buffer (pH 6.8): First step including the use of ammonium sulfate precipitation, second step utilize dialysis with acetate buffer, while the third step consists of gel-filtration on Sephacryl S200(27×1.6cm) . Insufficiently purified ceruloplasmin fractions obtained at various stages are diluted to the appropriate ionic strength and re-utilized. Two proteinous components had been isolated by gel filtration chromatography from the supernatant produced by ammonium sulfate. According to Poly Acryl Amide Electrophoresis PAGE appear two bands by protein staining. Abbreviation: ceruloplasmin, chronic renal failure, ammonium sulfate, dialysis, electrophoresis, gel filtration.

Published in	Advances in Biochemistry (Volume 2, Issue 6)
DOI	10.11648/j.ab.20140206.12
Page(s)	90-94
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Ceruloplasmin, Chronic Renal Failure, Ammonium Sulfate, Dialysis, Electrophoresis, Gel Filtration

References

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APA Style

Israa Ghassan Zainal. (2014). Estimation Activity and Partial Purification of Ceruloplasmin from Sera of Patients with Chronic Renal Failure and Healthy Subjects. Advances in Biochemistry, 2(6), 90-94. https://doi.org/10.11648/j.ab.20140206.12

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ACS Style

Israa Ghassan Zainal. Estimation Activity and Partial Purification of Ceruloplasmin from Sera of Patients with Chronic Renal Failure and Healthy Subjects. Adv. Biochem. 2014, 2(6), 90-94. doi: 10.11648/j.ab.20140206.12

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AMA Style

Israa Ghassan Zainal. Estimation Activity and Partial Purification of Ceruloplasmin from Sera of Patients with Chronic Renal Failure and Healthy Subjects. Adv Biochem. 2014;2(6):90-94. doi: 10.11648/j.ab.20140206.12

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@article{10.11648/j.ab.20140206.12,
  author = {Israa Ghassan Zainal},
  title = {Estimation Activity and Partial Purification of Ceruloplasmin from Sera of Patients with Chronic Renal Failure and Healthy Subjects},
  journal = {Advances in Biochemistry},
  volume = {2},
  number = {6},
  pages = {90-94},
  doi = {10.11648/j.ab.20140206.12},
  url = {https://doi.org/10.11648/j.ab.20140206.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20140206.12},
  abstract = {CeruloplasminCP (EC1.16.3.1) activity has been assayed in (32) serum samples of patients with Chronic Renal Failure CRF, and (32) serum samples of healthy individuals without any clinically detectable diseases have been used as control group. The aim of this study is to measure CP activity and partially purifying the enzyme from sera of patients with CRF and healthy individuals. The results of this study revealed that CP activity of patient’s serum shows a highly significant decrease (p < 0.05) compared to that of the healthy individuals. Ceruloplasmin enzyme was partially purified from the serum of patient with CRF and and healthy individual by three steps which operates throughout under mild conditions 4°, acetate buffer (pH 6.8): First step including the use of ammonium sulfate precipitation, second step utilize dialysis with acetate buffer, while the third step consists of gel-filtration on Sephacryl S200(27×1.6cm) . Insufficiently purified ceruloplasmin fractions obtained at various stages are diluted to the appropriate ionic strength and re-utilized. Two proteinous components had been isolated by gel filtration chromatography from the supernatant produced by ammonium sulfate. According to Poly Acryl Amide Electrophoresis PAGE appear two bands by protein staining. Abbreviation: ceruloplasmin, chronic renal failure, ammonium sulfate, dialysis, electrophoresis, gel filtration.},
 year = {2014}
}

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TY  - JOUR
T1  - Estimation Activity and Partial Purification of Ceruloplasmin from Sera of Patients with Chronic Renal Failure and Healthy Subjects
AU  - Israa Ghassan Zainal
Y1  - 2014/11/29
PY  - 2014
N1  - https://doi.org/10.11648/j.ab.20140206.12
DO  - 10.11648/j.ab.20140206.12
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 90
EP  - 94
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20140206.12
AB  - CeruloplasminCP (EC1.16.3.1) activity has been assayed in (32) serum samples of patients with Chronic Renal Failure CRF, and (32) serum samples of healthy individuals without any clinically detectable diseases have been used as control group. The aim of this study is to measure CP activity and partially purifying the enzyme from sera of patients with CRF and healthy individuals. The results of this study revealed that CP activity of patient’s serum shows a highly significant decrease (p < 0.05) compared to that of the healthy individuals. Ceruloplasmin enzyme was partially purified from the serum of patient with CRF and and healthy individual by three steps which operates throughout under mild conditions 4°, acetate buffer (pH 6.8): First step including the use of ammonium sulfate precipitation, second step utilize dialysis with acetate buffer, while the third step consists of gel-filtration on Sephacryl S200(27×1.6cm) . Insufficiently purified ceruloplasmin fractions obtained at various stages are diluted to the appropriate ionic strength and re-utilized. Two proteinous components had been isolated by gel filtration chromatography from the supernatant produced by ammonium sulfate. According to Poly Acryl Amide Electrophoresis PAGE appear two bands by protein staining. Abbreviation: ceruloplasmin, chronic renal failure, ammonium sulfate, dialysis, electrophoresis, gel filtration.
VL  - 2
IS  - 6
ER  -

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Author Information

Israa Ghassan Zainal

Department of Chemistry, College of Science, Al-Mustansiryh University, Baghdad, Iraq

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APA Style

Israa Ghassan Zainal. (2014). Estimation Activity and Partial Purification of Ceruloplasmin from Sera of Patients with Chronic Renal Failure and Healthy Subjects. Advances in Biochemistry, 2(6), 90-94. https://doi.org/10.11648/j.ab.20140206.12

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ACS Style

Israa Ghassan Zainal. Estimation Activity and Partial Purification of Ceruloplasmin from Sera of Patients with Chronic Renal Failure and Healthy Subjects. Adv. Biochem. 2014, 2(6), 90-94. doi: 10.11648/j.ab.20140206.12

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AMA Style

Israa Ghassan Zainal. Estimation Activity and Partial Purification of Ceruloplasmin from Sera of Patients with Chronic Renal Failure and Healthy Subjects. Adv Biochem. 2014;2(6):90-94. doi: 10.11648/j.ab.20140206.12

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@article{10.11648/j.ab.20140206.12,
  author = {Israa Ghassan Zainal},
  title = {Estimation Activity and Partial Purification of Ceruloplasmin from Sera of Patients with Chronic Renal Failure and Healthy Subjects},
  journal = {Advances in Biochemistry},
  volume = {2},
  number = {6},
  pages = {90-94},
  doi = {10.11648/j.ab.20140206.12},
  url = {https://doi.org/10.11648/j.ab.20140206.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20140206.12},
  abstract = {CeruloplasminCP (EC1.16.3.1) activity has been assayed in (32) serum samples of patients with Chronic Renal Failure CRF, and (32) serum samples of healthy individuals without any clinically detectable diseases have been used as control group. The aim of this study is to measure CP activity and partially purifying the enzyme from sera of patients with CRF and healthy individuals. The results of this study revealed that CP activity of patient’s serum shows a highly significant decrease (p < 0.05) compared to that of the healthy individuals. Ceruloplasmin enzyme was partially purified from the serum of patient with CRF and and healthy individual by three steps which operates throughout under mild conditions 4°, acetate buffer (pH 6.8): First step including the use of ammonium sulfate precipitation, second step utilize dialysis with acetate buffer, while the third step consists of gel-filtration on Sephacryl S200(27×1.6cm) . Insufficiently purified ceruloplasmin fractions obtained at various stages are diluted to the appropriate ionic strength and re-utilized. Two proteinous components had been isolated by gel filtration chromatography from the supernatant produced by ammonium sulfate. According to Poly Acryl Amide Electrophoresis PAGE appear two bands by protein staining. Abbreviation: ceruloplasmin, chronic renal failure, ammonium sulfate, dialysis, electrophoresis, gel filtration.},
 year = {2014}
}

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TY  - JOUR
T1  - Estimation Activity and Partial Purification of Ceruloplasmin from Sera of Patients with Chronic Renal Failure and Healthy Subjects
AU  - Israa Ghassan Zainal
Y1  - 2014/11/29
PY  - 2014
N1  - https://doi.org/10.11648/j.ab.20140206.12
DO  - 10.11648/j.ab.20140206.12
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 90
EP  - 94
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20140206.12
AB  - CeruloplasminCP (EC1.16.3.1) activity has been assayed in (32) serum samples of patients with Chronic Renal Failure CRF, and (32) serum samples of healthy individuals without any clinically detectable diseases have been used as control group. The aim of this study is to measure CP activity and partially purifying the enzyme from sera of patients with CRF and healthy individuals. The results of this study revealed that CP activity of patient’s serum shows a highly significant decrease (p < 0.05) compared to that of the healthy individuals. Ceruloplasmin enzyme was partially purified from the serum of patient with CRF and and healthy individual by three steps which operates throughout under mild conditions 4°, acetate buffer (pH 6.8): First step including the use of ammonium sulfate precipitation, second step utilize dialysis with acetate buffer, while the third step consists of gel-filtration on Sephacryl S200(27×1.6cm) . Insufficiently purified ceruloplasmin fractions obtained at various stages are diluted to the appropriate ionic strength and re-utilized. Two proteinous components had been isolated by gel filtration chromatography from the supernatant produced by ammonium sulfate. According to Poly Acryl Amide Electrophoresis PAGE appear two bands by protein staining. Abbreviation: ceruloplasmin, chronic renal failure, ammonium sulfate, dialysis, electrophoresis, gel filtration.
VL  - 2
IS  - 6
ER  -

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