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Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic

Received: 5 March 2013     Published: 20 June 2013
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Abstract

Optimization of exopolysaccharides (EPS) produced by three strains of entomopathogenic fungi (Beauveria bassiana BCC 2692, Ophiocordyceps dipterigena BCC 2073, and Paecilomyces tenuipes BCC 2656) was carried out together with analyses of their prebiotic properties. B. bassiana BCC 2692 produced 6.27±0.22 g/L EPS on optimal medium using two-level fractional factorial design and 4.7 g/L EPS in bioreactor. EPS productions of O. dipterigena BCC 2073 were 13.2 g/L and 41.2 g/L in shake flask and bioreactor, respectively. For P. tenuipes BCC 2656, 1.47±0.21 g/L EPS in shake flask and 28.1 g/L EPS in bioreactor were obtained. These EPS were previously characterized as -glucan with differences in molecular weights and degree of branching. They were resistant to hydrolysis by both hydrochloric acid and porcine pancreatic α-amylase. Furthermore, when used as the sole carbon source, all three types of EPS supported growth in vitro of two different probiotic bacteria (Lactobacillus acidoplilus BCC 13839 and Bifidobacterium animalis ATCC 25527). A constant viability of L. acidophilus BCC 13839 was maintained throughout the cultivation period (48 hours) on all three entomopatogenic fungal EPS. All EPS also supported better growth and maintained longer growth period of B. animalis ATCC 25527 than glucose or inulin. Thus these entomopathogenic fungi EPS are promising candidates in prebiotic industry, expanding the pool of current commercial prebiotics.

Published in Advances in Biochemistry (Volume 1, Issue 2)
DOI 10.11648/j.ab.20130102.12
Page(s) 13-21
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Exopolysaccharide, Glucan, Prebiotic, Probiotic, Entomopathogenic Fungi

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    Wai Prathumpai, Pranee Rachathewee, Sutamat Khajeeram, Jean-Jacques Sanglier, Pariyada Tanjak, et al. (2013). Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic. Advances in Biochemistry, 1(2), 13-21. https://doi.org/10.11648/j.ab.20130102.12

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    Wai Prathumpai; Pranee Rachathewee; Sutamat Khajeeram; Jean-Jacques Sanglier; Pariyada Tanjak, et al. Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic. Adv. Biochem. 2013, 1(2), 13-21. doi: 10.11648/j.ab.20130102.12

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    AMA Style

    Wai Prathumpai, Pranee Rachathewee, Sutamat Khajeeram, Jean-Jacques Sanglier, Pariyada Tanjak, et al. Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic. Adv Biochem. 2013;1(2):13-21. doi: 10.11648/j.ab.20130102.12

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  • @article{10.11648/j.ab.20130102.12,
      author = {Wai Prathumpai and Pranee Rachathewee and Sutamat Khajeeram and Jean-Jacques Sanglier and Pariyada Tanjak and Pawadee Methacanon},
      title = {Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic},
      journal = {Advances in Biochemistry},
      volume = {1},
      number = {2},
      pages = {13-21},
      doi = {10.11648/j.ab.20130102.12},
      url = {https://doi.org/10.11648/j.ab.20130102.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20130102.12},
      abstract = {Optimization of exopolysaccharides (EPS) produced by three strains of entomopathogenic fungi (Beauveria bassiana BCC 2692, Ophiocordyceps dipterigena BCC 2073, and Paecilomyces tenuipes BCC 2656) was carried out together with analyses of their prebiotic properties. B. bassiana BCC 2692 produced 6.27±0.22 g/L EPS on optimal medium using two-level fractional factorial design and 4.7 g/L EPS in bioreactor. EPS productions of O. dipterigena BCC 2073 were 13.2 g/L and 41.2 g/L in shake flask and bioreactor, respectively. For P. tenuipes BCC 2656, 1.47±0.21 g/L EPS in shake flask and 28.1 g/L EPS in bioreactor were obtained. These EPS were previously characterized as -glucan with differences in molecular weights and degree of branching. They were resistant to hydrolysis by both hydrochloric acid and porcine pancreatic α-amylase. Furthermore, when used as the sole carbon source, all three types of EPS supported growth in vitro of two different probiotic bacteria (Lactobacillus acidoplilus BCC 13839 and Bifidobacterium animalis ATCC 25527). A constant viability of L. acidophilus BCC 13839 was maintained throughout the cultivation period (48 hours) on all three entomopatogenic fungal EPS. All EPS also supported better growth and maintained longer growth period of B. animalis ATCC 25527 than glucose or inulin. Thus these entomopathogenic fungi EPS are promising candidates in prebiotic industry, expanding the pool of current commercial prebiotics.},
     year = {2013}
    }
    

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  • TY  - JOUR
    T1  - Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic
    AU  - Wai Prathumpai
    AU  - Pranee Rachathewee
    AU  - Sutamat Khajeeram
    AU  - Jean-Jacques Sanglier
    AU  - Pariyada Tanjak
    AU  - Pawadee Methacanon
    Y1  - 2013/06/20
    PY  - 2013
    N1  - https://doi.org/10.11648/j.ab.20130102.12
    DO  - 10.11648/j.ab.20130102.12
    T2  - Advances in Biochemistry
    JF  - Advances in Biochemistry
    JO  - Advances in Biochemistry
    SP  - 13
    EP  - 21
    PB  - Science Publishing Group
    SN  - 2329-0862
    UR  - https://doi.org/10.11648/j.ab.20130102.12
    AB  - Optimization of exopolysaccharides (EPS) produced by three strains of entomopathogenic fungi (Beauveria bassiana BCC 2692, Ophiocordyceps dipterigena BCC 2073, and Paecilomyces tenuipes BCC 2656) was carried out together with analyses of their prebiotic properties. B. bassiana BCC 2692 produced 6.27±0.22 g/L EPS on optimal medium using two-level fractional factorial design and 4.7 g/L EPS in bioreactor. EPS productions of O. dipterigena BCC 2073 were 13.2 g/L and 41.2 g/L in shake flask and bioreactor, respectively. For P. tenuipes BCC 2656, 1.47±0.21 g/L EPS in shake flask and 28.1 g/L EPS in bioreactor were obtained. These EPS were previously characterized as -glucan with differences in molecular weights and degree of branching. They were resistant to hydrolysis by both hydrochloric acid and porcine pancreatic α-amylase. Furthermore, when used as the sole carbon source, all three types of EPS supported growth in vitro of two different probiotic bacteria (Lactobacillus acidoplilus BCC 13839 and Bifidobacterium animalis ATCC 25527). A constant viability of L. acidophilus BCC 13839 was maintained throughout the cultivation period (48 hours) on all three entomopatogenic fungal EPS. All EPS also supported better growth and maintained longer growth period of B. animalis ATCC 25527 than glucose or inulin. Thus these entomopathogenic fungi EPS are promising candidates in prebiotic industry, expanding the pool of current commercial prebiotics.
    VL  - 1
    IS  - 2
    ER  - 

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Author Information
  • National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand

  • National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand

  • Department of Biotechnology, Thammasat University, Klong Nueng, Klong Luang, Pathumthani 12120, Thailand

  • Novartis Institute for BioMedical Research Basel, Natural Products Unit, Novartis Pharma AG, Basel, Switzerland

  • National Metal and Materials Technology Center (MTEC), 114 Thailand Science Park, Paholyothin Rd., Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand

  • National Metal and Materials Technology Center (MTEC), 114 Thailand Science Park, Paholyothin Rd., Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand

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