Distillation environments represents a rich resource of microbial diversity, some of which are of high value to industry. This study was undertaken to isolate, screen and identify naturally evolved indigenous yeast with ethanol tolerant capabilities. Data obtained revealed that using 0.2% Chloramphenicol supplemented potato dextrose media, yeast isolates were attainable from soil samples obtained from a local distillery in Bayelsa state, Nigeria. The obtained colonies were capable of fermenting glucose, sucrose, fructose and galactose giving off distinct yellow colour using phenol red broth method. Morphological examination revealed that the isolates obtained were white, round shaped, smooth textured and flat elevation with transparent opacity. A dip in its growth curve was observed in broth cultures consisting of yeast extract, peptone, malt extract, glucose containing 10-20% (v/v) absolute ethanol between 24-48 hours of incubation at 30°C. In this broth cultures, a progressive growth curve was observed between 48-120 hours at the same parameters for incubation. The isolate also demonstrated good growth in ethanol supplemented medium with pH ranging from 5.2-6.6 at 30°C. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 570nm. The basic local alignment search tool (BLAST) of the genetic sequence obtained revealed a 98% similarity to Meyerozyma guilliermondii. The results obtained suggested that non-Saccharomyces species possess ethanol tolerant ability, particularly those obtained from alcohol rich environments. Such yeast species could be applied towards the fermentation of ethanol for industrial uses.
| Published in | Advances in Applied Sciences (Volume 1, Issue 3) |
| DOI | 10.11648/j.aas.20160103.15 |
| Page(s) | 78-85 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Ethanol, Ethanol Tolerance, Saccharomyces cerevisiae, Non-saccharomyces, Meyerozyma guilliermondii
| [1] | Lin, Y., and Tanaka, S. (2006). Ethanol fermentation from biomass resources: current state and prospects. Applied Microbiology and Biotechnology, 69:627–642. |
| [2] | D'Amore, T., Panchal, C. J., Russell, I., and Stewart, G. G. (1990). A study of ethanol tolerance in yeast. Critical Reviews Biotechnology, 9: 287–304. |
| [3] | Bauer, F. F., and Pretorius, I. (2000). Yeast stress response and fermentation efficiency: how to survive the making of wine-a review. South African Journal of Enology and Viticulture, 21: 27-51. |
| [4] | Bai, F. W., Chen, L. J., Zhang, Z., Anderson, W. A., and Moo-Young, M. (2004). Continuous ethanol production and evaluation of yeast cell lysis and viability loss under very high gravity medium conditions. Journal of Biotechnology, 110: 287–293. |
| [5] | Balat, M., Balat, H., and Oz, C. (2008). Progress in bioethanol processing. Progress in Energy and Combustion Science, 34: 551–573. |
| [6] | Folch-Mallol, J., Garay-Arroyo, A., Lledías, F., Covarrubias-Robles, A. 2004. The stress response in the yeast Saccharomyces cerevisiae. Revista Latinoamericana de Microbiologia, 46: 24-46. |
| [7] | Brosnan, M. P., Donnelly, D., James, T. C., and Bond, U. (2000). The stress response is repressed during fermentation in brewery strains of yeast. Journal of Applied Microbiology, 88: 746-755. |
| [8] | Steensma, H. Y., and Ter-Linde, J. J. (2001). Plasmids with the Crerecombinase and the dominant nat marker, suitable for use in prototrophic strains of S. cerevisiae and Kluyveromyces lactis. Yeast, 18: 469-472. |
| [9] | Jolly, N. P., Augustyn, O. P. H., and Pretorius, I. S. (2003). The effect of non-Saccharomyces yeasts on fermentation and wine quality. South African Journal of Enology and Viticulture, 24: 55-62. |
| [10] | Matsushika A., Inoue, H., Kodaki, T., and Sawayama, S. (2009). Ethanol production from xylose in engineered Saccharomyces cerevisiae strains: current state and perspectives. Applied Microbiology and Biotechnology, 84:37–53. |
| [11] | Ciani, M., Comitini, F., Mannazzu, I., and Domizio, P. (2010). Controlled mixed culture fermentation: a new perspective on the use of non-Saccharomyces yeasts in winemaking. FEMS Yeast Research, 10:123–133. |
| [12] | Sedlak, M., and Ho, NW. (2004). Production of ethanol from cellulosic biomass hydrolysates using genetically engineered saccharomyces yeast capable of cofermenting glucose and xylose. Applied Biochemistry and Biotechnology, 114: 403-416. |
| [13] | Agbogbo, FK., Coward-Kelly, G., Torry-Smith, M., and Wenger, KS. (2006). Fermentation of glucose/xylose mixtures using Pichia stipitis. Process Biochemistry, 41:2333-2336. |
| [14] | Agbogbo FK, and Coward-Kelly G (2008) Cellulosic ethanol production using the naturally occurring xylose-fermenting yeast, Pichia stipitis. Biotechnology Letters, 30:1515-1524. |
| [15] | Wilkins, MR., Suryawati, L., and Chrz, D. (2007). Ethanol production by Saccharomyces cerevisiae and Kluyveromyces marxianus in the presence of orange peel oil. World Journal of Microbiology and Biotechnology, 23: 1161-1168. |
| [16] | Li, JR., and Cai, AQ. (2007). Isolation and identification of main microorganisms in traditional distiller's yeast. Liquor-Making Science Technoly., 5: 111-115 (In Chinese with English abstract). |
| [17] | Tao N., Gao, Y., and Liu, Y., (2011). Isolation and characterization of a Pichia anomala strain: a promising candidate for bioethanol production. Brazilian Journal of Microbiology, 42: 668-675 |
| [18] | Gidado, RSM., Etim, VA., Nweke, O., Iloh, AC., Isu, RN., and Solomon, BO., (2016). Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria. American Journal of BioScience, 4: 58-63 |
| [19] | Barnett, JA., Payne, RW., and Yarrow, D. (2000). Yeasts: Characteristics and identification. 3rd edn. Cambrie University Press, UK. ISBN-13:978-0521573962 |
| [20] | Khaing, TW., Weine, N., and Mya, MO. (2008). Isolation, Characterization and Screening of Thermo tolerant, Ethanol Tolerant Indigenous Yeasts and Study on the Effectiveness of Immobilized Cell for Ethanol Production. Journal of Science and Technology, 1: 12-14. |
| [21] | Caggia, C., Restuccia, C., Pulvirenti, A., and Giudici, P. (2001). Identification of Pichia anomala isolated from yoghurt by RFLP of the ITS region. International Journal of Food Microbiology, 71: 71-73. |
| [22] | Meersman, E., Steensels, J., Mathawan M., et al., (2013). Detailed analysis of the microbial population in Malaysian spontaneous cocoa pulp fermentations reveals a core and variable microbiota. PLoS One, 8: e81559. |
| [23] | Bokulich, NA., Thorngate, JH., Richardson, PM., et al., (2014). Microbial biogeography of wine grapes is conditioned by cultivar, vintage, and climate. P Natl Acad Sci USA, 111: 139–148. |
| [24] | Steensels, J., and Verstrepen, KJ., (2014). Taming wild yeast: potential of conventional and nonconventional yeasts in industrial fermentations. Annu Review Microbiology, 68: 61–80. |
| [25] | Basso, LC., Basso, TO., and Rocha, SN., (2011). Ethanol Production in Brazil: The Industrial Process and Its Impact on Yeast Fermentation. In: dos Santos Bernardes MA (ed). Biofuel production—recent developments and prospects. InTech, 1530, |
| [26] | Taylor, MP., Mulako, I., Tuffin, M., et al., (2012). Understanding physiological responses to pre-treatment inhibitors in ethanologenic fermentations. Biotechnology Journal, 7:1169–1181. |
| [27] | Souciet, JL., Dujon, B., Gaillardin, C., et al., (2009). Comparative genomics of protoploid Saccharomycetaceae. Genome Research, 19:1696–1709. |
| [28] | Martorell, P., Stratford, M., Steels, H., et al., (2007). Physiological characterization of spoilage strains of Zygosaccharomyces bailii and Zygosaccharomyces rouxii isolated from high sugar environments. International Journal of Food Microbiology, 114:234–242. |
| [29] | Dujon, B., (2010). Yeast evolutionary genomics. Nature Reviews Genetics, 11: 512–524. |
| [30] | Kurtzman, CP., (2011). A new methanol assimilating yeast, Ogataea parapolymorpha, the ascosporic state of Candida parapolymorpha. Anton Leeuw, 100: 455–462. |
| [31] | Dashko, S., Zhou, N., Compagno, C., et al., (2014). Why, when, and how did yeast evolve alcoholic fermentation? FEMS Yeast Research, 14: 826–832. |
| [32] | Kurylenko, OO., Ruchala, J., Hryniv, OB., et al., (2014). Metabolic engineering and classical selection of the methylotrophic thermotolerant yeast Hansenula polymorpha for improvement of high-temperature xylose alcoholic fermentation. Microbiology Cell Factor; 13:122. |
| [33] | Meroth, CB., Hammes, WP., and Hertel, C., (2003). Identification and population dynamics of Yeasts in sourdough fermentation processes by PCR-denaturing gradient gel electrophoresis. Applied Environmental Microbiology, 69:7453–7461. |
| [34] | Dandi, ND., Dandi, BN., and Chaudhari, AB., (2013). Bioprospecting of thermo and osmo-tolerant fungi from mango pulp-peel compost for bioethanol production. Anton Leeuw, 103: 723–736. |
| [35] | Dhaliwal, SS., Oberoi, HS., Sandhu, SK., et al., (2011). Enhanced ethanol production from sugarcane juice by galactose adaptation of a newly isolated thermotolerant strain of Pichia kudriavzevii. Bioresource Technology, 102: 5968–5975. |
| [36] | Chan, GF., Gan, HM., Ling, HL., et al., (2012). Genome sequence of Pichia kudriavzevii M12, a potential producer of bioethanol and phytase. Eukaryotic Cell, 11:1300–1301. |
| [37] | Mukherjee, V., Steensels, J., Lievens, B., et al., (2014). Phenotypic evaluation of natural and industrial Saccharomyces yeasts for different traits desirable in industrial bioethanol production. Applied Microbiology and Biotechnology, 98:9483–9498. |
| [38] | Schnierda, T., Bauer, FF., Divol, B., et al., (2014). Optimization of carbon and nitrogen medium components for biomass production using non-Saccharomyces wine yeasts. Letters Applied Microbiology, 58: 478–485. |
| [39] | Mishima, D., Kuniki, M., Sei, K., Soda, S., Ike, M., and Fujita, M., (2008). Ethanol production from candidate energy crops: water hyacinth (Eichhornia crassipes) and water lettuce (Pistia stratiotes L.). Bioresource Technology, 99: 2495–2500. |
| [40] | Olofsson, K., Rudolf, A., and Liden, G., (2008). Designing simultaneous saccharification and fermentation for improved xylose conversion by a recombinant strain of Saccharomyces cerevisiae. Journal of Biotechnology, 134: 112–120. |
| [41] | Tofalo, R., Chaves-Lopez, C., Fabio, F. D., Schirone, M., Felis, G. E., Torriani, S., et al., (2009). Molecular identification and osmotolerant profile of wine yeasts that ferment a high sugar grape must. International Journal of Food Microbiology, 130: 179-187. |
| [42] | Galafassi, S., Merico, A., Pizza, F., et al., (2011). Dekkera/Brettanomyces yeasts for ethanol production from renewable sources under oxygen-limited and low-pH conditions. Journal of Industrial Microbiology and Biotechnology, 38: 1079–1088. |
| [43] | Zha, Y., Hossain, AH., Tobola, F., et al., (2013). Pichia anomala 29X: a resistant strain for lignocellulosic biomass hydrolysate fermentation. FEMS Yeast Research, 13: 609–617. |
| [44] | Ruyters, S., Mukherjee, V., Verstrepen, KJ., et al., (2015). Assessing the potential of wild yeasts for bioethanol production. Journal of Industrial Microbiology and Biotechnology, 42: 39–48. |
| [45] | Arroyo-Lopez, FN., Duran-Quintana, MC., Ruiz-Barba, JL., Querol, A., and Garrido-Fernandez, A., (2006). Use of molecular methods for the identification of yeast associated with table olives, Food Microbiology, 23; 791–796. |
| [46] | Kurtzman, CP., and Suzuki, M., (2010). Phylogenetic analysis of ascomycete yeasts that form coenzyme Q-9 and the proposal of the new genera Babjeviella, Meyerozyma, Millerozyma, Priceomyces, and Scheffersomyces. Mycoscience Tokyo, 51: 2–14. |
| [47] | Oberoi, HS., Babbar, N., Sandhu, SK., et al., (2012). Ethanol production from alkali-treated rice straw via simultaneous saccharification and fermentation using newly isolated thermotolerant Pichia kudriavzevii HOP-1. Journal of Industrial Microbiology and Biotechnology, 39:557–566. |
| [48] | Brett, CL., Tukaye, DN., Mukherjee, S., et al., (2005). The yeast endosomal Na+(K+)/H+ exchanger Nhx1 regulates cellular pH to control vesicle trafficking. Cell Molecular Biology, 16: 1396–1405. |
| [49] | Mollapour, M., and Piper, PW., (2007). Hog1 Mitogen-activated protein kinase phosphorylation targets the yeast Fps1 aqua glyceroporin for endocytosis, thereby rendering cells resistant to acetic acid. Molecular Cell Biology, 27:6446–6456. |
| [50] | Lindberg, L., Santos, AXS., Riezman, H., et al., (2013). Lipidomic profiling of Saccharomyces cerevisiae and Zygosaccharomyces bailii reveals critical changes in lipid composition in response to acetic acid stress. PLoS One, 8: |
| [51] | Rajoka, M., Khalid A, and Ferhan, M. (2005). Kinetic and thermodynamics of ethanol production by a thermotolerant mutant of Saccharomyces cerevisiae. Letters in Applied Microbiology, 40: 316–21. |
APA Style
Gidado Rose Suniso Maxwell, Olatiilu Olukemi Anna, Etuk-Udo Godwin Akpan, Onyenekwe Paul Chidozie, Isu Rosemary Nennaya, et al. (2016). Isolation and Characterization of Yeast Inhabiting Alcohol Processing Environment in Bayelsa State, Nigeria. Advances in Applied Sciences, 1(3), 78-85. https://doi.org/10.11648/j.aas.20160103.15
ACS Style
Gidado Rose Suniso Maxwell; Olatiilu Olukemi Anna; Etuk-Udo Godwin Akpan; Onyenekwe Paul Chidozie; Isu Rosemary Nennaya, et al. Isolation and Characterization of Yeast Inhabiting Alcohol Processing Environment in Bayelsa State, Nigeria. Adv. Appl. Sci. 2016, 1(3), 78-85. doi: 10.11648/j.aas.20160103.15
AMA Style
Gidado Rose Suniso Maxwell, Olatiilu Olukemi Anna, Etuk-Udo Godwin Akpan, Onyenekwe Paul Chidozie, Isu Rosemary Nennaya, et al. Isolation and Characterization of Yeast Inhabiting Alcohol Processing Environment in Bayelsa State, Nigeria. Adv Appl Sci. 2016;1(3):78-85. doi: 10.11648/j.aas.20160103.15
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author = {Gidado Rose Suniso Maxwell and Olatiilu Olukemi Anna and Etuk-Udo Godwin Akpan and Onyenekwe Paul Chidozie and Isu Rosemary Nennaya and Habu Josiah},
title = {Isolation and Characterization of Yeast Inhabiting Alcohol Processing Environment in Bayelsa State, Nigeria},
journal = {Advances in Applied Sciences},
volume = {1},
number = {3},
pages = {78-85},
doi = {10.11648/j.aas.20160103.15},
url = {https://doi.org/10.11648/j.aas.20160103.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aas.20160103.15},
abstract = {Distillation environments represents a rich resource of microbial diversity, some of which are of high value to industry. This study was undertaken to isolate, screen and identify naturally evolved indigenous yeast with ethanol tolerant capabilities. Data obtained revealed that using 0.2% Chloramphenicol supplemented potato dextrose media, yeast isolates were attainable from soil samples obtained from a local distillery in Bayelsa state, Nigeria. The obtained colonies were capable of fermenting glucose, sucrose, fructose and galactose giving off distinct yellow colour using phenol red broth method. Morphological examination revealed that the isolates obtained were white, round shaped, smooth textured and flat elevation with transparent opacity. A dip in its growth curve was observed in broth cultures consisting of yeast extract, peptone, malt extract, glucose containing 10-20% (v/v) absolute ethanol between 24-48 hours of incubation at 30°C. In this broth cultures, a progressive growth curve was observed between 48-120 hours at the same parameters for incubation. The isolate also demonstrated good growth in ethanol supplemented medium with pH ranging from 5.2-6.6 at 30°C. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 570nm. The basic local alignment search tool (BLAST) of the genetic sequence obtained revealed a 98% similarity to Meyerozyma guilliermondii. The results obtained suggested that non-Saccharomyces species possess ethanol tolerant ability, particularly those obtained from alcohol rich environments. Such yeast species could be applied towards the fermentation of ethanol for industrial uses.},
year = {2016}
}
TY - JOUR T1 - Isolation and Characterization of Yeast Inhabiting Alcohol Processing Environment in Bayelsa State, Nigeria AU - Gidado Rose Suniso Maxwell AU - Olatiilu Olukemi Anna AU - Etuk-Udo Godwin Akpan AU - Onyenekwe Paul Chidozie AU - Isu Rosemary Nennaya AU - Habu Josiah Y1 - 2016/11/19 PY - 2016 N1 - https://doi.org/10.11648/j.aas.20160103.15 DO - 10.11648/j.aas.20160103.15 T2 - Advances in Applied Sciences JF - Advances in Applied Sciences JO - Advances in Applied Sciences SP - 78 EP - 85 PB - Science Publishing Group SN - 2575-1514 UR - https://doi.org/10.11648/j.aas.20160103.15 AB - Distillation environments represents a rich resource of microbial diversity, some of which are of high value to industry. This study was undertaken to isolate, screen and identify naturally evolved indigenous yeast with ethanol tolerant capabilities. Data obtained revealed that using 0.2% Chloramphenicol supplemented potato dextrose media, yeast isolates were attainable from soil samples obtained from a local distillery in Bayelsa state, Nigeria. The obtained colonies were capable of fermenting glucose, sucrose, fructose and galactose giving off distinct yellow colour using phenol red broth method. Morphological examination revealed that the isolates obtained were white, round shaped, smooth textured and flat elevation with transparent opacity. A dip in its growth curve was observed in broth cultures consisting of yeast extract, peptone, malt extract, glucose containing 10-20% (v/v) absolute ethanol between 24-48 hours of incubation at 30°C. In this broth cultures, a progressive growth curve was observed between 48-120 hours at the same parameters for incubation. The isolate also demonstrated good growth in ethanol supplemented medium with pH ranging from 5.2-6.6 at 30°C. Growth measurements were determined by measuring optical density of the cells in broth using spectrophotometer at 570nm. The basic local alignment search tool (BLAST) of the genetic sequence obtained revealed a 98% similarity to Meyerozyma guilliermondii. The results obtained suggested that non-Saccharomyces species possess ethanol tolerant ability, particularly those obtained from alcohol rich environments. Such yeast species could be applied towards the fermentation of ethanol for industrial uses. VL - 1 IS - 3 ER -
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