Lycopene is the red pigment richly found in many red -colored fruits and vegetables such as tomatoes, papaya, pink grapefruit, pink guava, and watermelon. It is an antioxidant that has various beneficent effects and protects against oxidative damage to lipids, protein, and DNA. It is a potent quencher of oxygen and most effective against cancer, cardiovascular diseases, osteoporosis, hypertension, male infertility, and neurodegenerative disease. Lycopene is synthesized by plants and microorganisms but not animals and humans. The main objective of this study is to develop and validate an HPLC assay method for the determination of lycopene in tomato, watermelon, and human blood. A simple assay method is an economical assay method, suitable for the determination of lycopene from plant and biological sources. A new accurate, specific, precise, high performance liquid chromatographic (HPLC) method has been developed and validated for the determination of lycopene in tomatoes, watermelon and blood sample according to the International Conference on Harmonization (ICH) guidelines. Acetonitrile, Methanol, Water (65%, 20%, and 15%) was used as the mobile phase at a flow rate of 0.7ml/min. using a symmetry C18 column. The effluent was spectrophotometrically monitored at 484nm. The calibration curve was linear with a correlation coefficient of 0.9994. The average recovery was found to be 102.0%. The proposed HPLC method was successfully applied to quantify the amount of lycopene in different matrices.
| Published in | World Journal of Applied Chemistry (Volume 5, Issue 2) |
| DOI | 10.11648/j.wjac.20200502.13 |
| Page(s) | 26-33 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2020. Published by Science Publishing Group |
HPLC, Lycopene, C18, Correlation Coefficient, Validation
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APA Style
Muhammad Amjad, Shabbir Hussain, Abdul Rehman Khan. (2020). Development and Validation of HPLC assay of Lycopene in Different Matrices. World Journal of Applied Chemistry, 5(2), 26-33. https://doi.org/10.11648/j.wjac.20200502.13
ACS Style
Muhammad Amjad; Shabbir Hussain; Abdul Rehman Khan. Development and Validation of HPLC assay of Lycopene in Different Matrices. World J. Appl. Chem. 2020, 5(2), 26-33. doi: 10.11648/j.wjac.20200502.13
AMA Style
Muhammad Amjad, Shabbir Hussain, Abdul Rehman Khan. Development and Validation of HPLC assay of Lycopene in Different Matrices. World J Appl Chem. 2020;5(2):26-33. doi: 10.11648/j.wjac.20200502.13
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Download@article{10.11648/j.wjac.20200502.13,
author = {Muhammad Amjad and Shabbir Hussain and Abdul Rehman Khan},
title = {Development and Validation of HPLC assay of Lycopene in Different Matrices},
journal = {World Journal of Applied Chemistry},
volume = {5},
number = {2},
pages = {26-33},
doi = {10.11648/j.wjac.20200502.13},
url = {https://doi.org/10.11648/j.wjac.20200502.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.wjac.20200502.13},
abstract = {Lycopene is the red pigment richly found in many red -colored fruits and vegetables such as tomatoes, papaya, pink grapefruit, pink guava, and watermelon. It is an antioxidant that has various beneficent effects and protects against oxidative damage to lipids, protein, and DNA. It is a potent quencher of oxygen and most effective against cancer, cardiovascular diseases, osteoporosis, hypertension, male infertility, and neurodegenerative disease. Lycopene is synthesized by plants and microorganisms but not animals and humans. The main objective of this study is to develop and validate an HPLC assay method for the determination of lycopene in tomato, watermelon, and human blood. A simple assay method is an economical assay method, suitable for the determination of lycopene from plant and biological sources. A new accurate, specific, precise, high performance liquid chromatographic (HPLC) method has been developed and validated for the determination of lycopene in tomatoes, watermelon and blood sample according to the International Conference on Harmonization (ICH) guidelines. Acetonitrile, Methanol, Water (65%, 20%, and 15%) was used as the mobile phase at a flow rate of 0.7ml/min. using a symmetry C18 column. The effluent was spectrophotometrically monitored at 484nm. The calibration curve was linear with a correlation coefficient of 0.9994. The average recovery was found to be 102.0%. The proposed HPLC method was successfully applied to quantify the amount of lycopene in different matrices.},
year = {2020}
}
TY - JOUR T1 - Development and Validation of HPLC assay of Lycopene in Different Matrices AU - Muhammad Amjad AU - Shabbir Hussain AU - Abdul Rehman Khan Y1 - 2020/07/13 PY - 2020 N1 - https://doi.org/10.11648/j.wjac.20200502.13 DO - 10.11648/j.wjac.20200502.13 T2 - World Journal of Applied Chemistry JF - World Journal of Applied Chemistry JO - World Journal of Applied Chemistry SP - 26 EP - 33 PB - Science Publishing Group SN - 2637-5982 UR - https://doi.org/10.11648/j.wjac.20200502.13 AB - Lycopene is the red pigment richly found in many red -colored fruits and vegetables such as tomatoes, papaya, pink grapefruit, pink guava, and watermelon. It is an antioxidant that has various beneficent effects and protects against oxidative damage to lipids, protein, and DNA. It is a potent quencher of oxygen and most effective against cancer, cardiovascular diseases, osteoporosis, hypertension, male infertility, and neurodegenerative disease. Lycopene is synthesized by plants and microorganisms but not animals and humans. The main objective of this study is to develop and validate an HPLC assay method for the determination of lycopene in tomato, watermelon, and human blood. A simple assay method is an economical assay method, suitable for the determination of lycopene from plant and biological sources. A new accurate, specific, precise, high performance liquid chromatographic (HPLC) method has been developed and validated for the determination of lycopene in tomatoes, watermelon and blood sample according to the International Conference on Harmonization (ICH) guidelines. Acetonitrile, Methanol, Water (65%, 20%, and 15%) was used as the mobile phase at a flow rate of 0.7ml/min. using a symmetry C18 column. The effluent was spectrophotometrically monitored at 484nm. The calibration curve was linear with a correlation coefficient of 0.9994. The average recovery was found to be 102.0%. The proposed HPLC method was successfully applied to quantify the amount of lycopene in different matrices. VL - 5 IS - 2 ER -
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