Microbial Cells Force Spectroscopy by Atomic Force Microscopy: A Review

Livia Angeloni; Daniele Passeri; Melania Reggente; Fabrizio Pantanella; Diego Mantovani; Marco Rossi

doi:doi:10.11648/j.nsnm.20160201.13

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Microbial Cells Force Spectroscopy by Atomic Force Microscopy: A Review

Livia Angeloni, Daniele Passeri, Melania Reggente, Fabrizio Pantanella, Diego Mantovani, Marco Rossi

Published in Nanoscience and Nanometrology (Volume 2, Issue 1)

Received: 18 September 2015 Accepted: 14 October 2015 Published: 15 October 2015

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Abstract

Bacterial adhesion and biofilm formation are important phenomena which can produce both detrimental and beneficial effects in several fields. Research is thus focused on the modulation of the properties of material surfaces in order to design and develop substrates able to control bacterial adhesion process, which is the first trigger event of biofilm formation. Several theoretical predictions and experimental procedures have been developed to investigate the physical, chemical and biological mechanisms regulating the attachment of bacteria to solid substrates. Nevertheless, a comprehensive understanding has not been achieved yet, limiting the capability of individuating effective technological strategies to achieve the desired bacterial adhesion behavior. The development of new experimental procedures able to furnish deeper information about bacterial adhesion mechanism is thus needed. Microbial cell force spectroscopy (MCFS) is an atomic force microscopy (AFM) based technique, consisting in the detection of force-distance curves using particular probes obtained immobilizing bacterial cells at the free end of a flexible microcantilever, which allows the detection of the different kinds of cell-surface interaction forces. In this work, we review the state of the art in the development of MCFS, focusing on its working principle and applications. A brief description of the current models and conventional experimental procedures used to evaluate bacterial adhesion to surfaces is reported. Then, the instrumentation and the working principle, the current procedures used to prepare bacterial cells probes and the main applications of the technique are described with the aim of pointing out the advantages of the technique and the limits which still have to be overcome.

Published in	Nanoscience and Nanometrology (Volume 2, Issue 1)
DOI	10.11648/j.nsnm.20160201.13
Page(s)	30-40
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Atomic Force Microscopy, Force Spectroscopy, Adhesion, Bacteria

References

[1]	G. G. Geesey, “Bacterial behavior at surfaces,” Curr. Opin. Microbiol., vol. 4, pp. 296-300, 2001.
[2]	P. Lejeune, “Contamination of abiotic surfaces: what a colonizing bacterium sees and how to blur it,” Trends Microbiol., vol. 11, pp. 179-184, 2003.
[3]	D. J. Stickler and R. J. C. McLean, “Biomaterials associated infections: The scale of the problem,” Cells Mater., vol. 5, pp. 167-182, 1995.
[4]	J. D. Bryers, “Biologically active surfaces: Processes governing the formation and persistence of biofilms,” Biotechnol. Progr., vol. 3, pp. 57-68, 1987.
[5]	A. G. Gristina, “Biomaterial-centered infection: Microbial adhesion versus tissue integration,” Science, vol. 237, pp. 1588-1595, 1987.
[6]	W. R. Jarvis and W. J. Martone, “Predominant pathogens in hospital infections,” J. Antimicrob. Chemother., vol. 29, pp. 19-24, 1992.
[7]	D. Gravel, A. Matlow, M. Ofner-Agostini, M. Loeb, L. Johnston, E. Bryce, M. L. Sample, V. R. Roth, C. Goldman, and G. Taylor, “Predominant pathogens in hospital infections,” Am. J. Infect. Control., vol. 35, pp. 157-162, 2007.
[8]	J. T. Walker and C. W. Keevil, “Study of microbial biofilms using light microscope techniques,” Int. Biodeter. Biodegr., vol. 34, pp. 223-236, 1994.
[9]	R. Sheikholeslami, “Fouling mitigation in membrane processes,” Desalination, vol. 123, pp. 45-53, 1999.
[10]	J. O. Tobin, R. A. Swann, and C. L. Bartlett, “Isolation of legionella pneumophila from water systems: methods and preliminary results,” Br. Med. J., vol. 282, pp. 515-517, 1981.
[11]	H. A. Videla, Manual of biocorrosion. CRC Press, Boca Raton, FL, 1996.
[12]	Commission Regulation (EC) No 1048/2005.
[13]	S. Salminen, C. Bouley, M. C. Boutron-Ruault, J. H. Cummings, A. Franck, G. R. Gibson, E. Isolauri, M. C. Moreau, M. Roberfroid, and I. Rowland, “Functional food science and gastrointestinal physiology and function,” Br. J. Nutr., vol. 80, pp. S147-S171, 1998.
[14]	T. Rohwerder, T. Gehrke, K. Kinzler, and W. Sand, “Bioleaching review part A: Progress in bioleaching: Fundamentals and mechanisms of bacterial metal sulfide oxidation,” Appl. Microbiol. Biotechnol., vol. 63, pp. 239-248, 2003.
[15]	W. R. Bowen, N. Hilal, R. W. Lovitt, and C. J.Wright, “Direct measurement of the force of adhesion of a single biological cell using an atomic force microscope,” Colloid. Surface. A, vol. 136, pp. 231-234, 1998.
[16]	A. Razatos, Y. L. Ong, M. M. Sharma, and G. Georgiou, “Direct measurement of colloidal forces using an atomic force microscope,” Proc. Natl. Acad. Sci. USA, vol. 95, pp. 11059-11064, 1998.
[17]	T. R. Garrett, M. Bhakoo, and Z. Zhang, “Bacterial adhesion and biofilms on surfaces,” Prog. Nat. Sci., vol. 18, pp. 1049-1056, 2008.
[18]	E. J. Verwey and J. T. G. Overbeek, Theory of the stability of lyophobic colloids. Elsevier, Amsterdam, 1948.
[19]	B. V. Derjaguin and L. D. Landau, “Theory of the stability of strongly charged lyophobic sols and the adhesion of strongly charged particles in olutions of electrolytes,” Acta Physiochim. USSR, vol. 14, pp. 633-662, 1941.
[20]	K. C. Marshall, R. Stout, and R. Mitchell, “Mechanism of the initial events in the sorption of marine bacteria to surfaces,” J. Gen. Microbiol., vol. 68, pp. 337-348, 1971.
[21]	H. H. M. Rijnaarts, W. Norde, E. J. Bouwer, J. Lyklema, and A. J. B. Zehnder, “Reversibility and mechanism of bacterial adhesion,” Colloid. Surface. B, vol. 4, pp. 5-22, 1995.
[22]	M. Morra and C. Cassinelli, “Bacterial adhesion to polymer surfaces: a critical review of surface thermodynamic approaches,” J. Biomater. Sci. Polym. Ed., vol. 9, pp. 55-74, 1997.
[23]	M. Fletcher and J. H. Pringle, “The effect of surface free energy and medium surface tension on bacterial attachment to solid surfaces,” J.Colloid Interf. Sci., vol. 104, pp. 5-14, 1985.
[24]	I. H. Pratt-Terpstra, A. H. Weerkamp, and H. J. Busscher, “Adhesion of oral streptococci from a flowing suspension to uncoated and albumincoated surfaces,” J. Gen. Microbiol., vol. 133, pp. 3199-3206, 1987.
[25]	P. R. Rutter and B. Vincent, “Physicochemical interactions of the substratum, microorganisms, and the fluid phase,” in Microbial adhesion and aggregation (K. C. Marshall, ed.), pp. 21-38, Springer Berlin Heidelberg, 1984.
[26]	I. H. Pratt-Terpstra, A. H.Weerkamp, and H. J. Busscher, “On a relation between interfacial free energy-dependent and noninterfacial free energy dependent adherence of oral streptococci to solid substrata,” Curr. Microbiol., vol. 16, pp. 311-313, 1988.
[27]	C. van Oss, “Energetics of cell-cell and cell-biopolymer interactions,” Cell Biophys., vol. 14, pp. 1-16, 1989.
[28]	Y. H. An and R. J. Friedman, “Laboratory methods for studies of bacterial adhesion,” J. Microbiol. Meth., vol. 30, pp. 141-152, 1997.
[29]	F. Pantanella, P. Valenti, A. Frioni, T. Natalizi, L. Coltella, and F. Berlutti, “Biotimer assay, a new method for counting Staphylococcus spp. in biofilm without sample manipulation applied to evaluate antibiotic susceptibility of biofilm,” J. Microbiol. Meth., vol. 75, pp. 478-484, 2008.
[30]	F. Pantanella, P. Valenti, T. Natalizi, D. Passeri, and F. Berlutti, “Analytical techniques to study microbial biofilm on abiotic surfaces: pros and cons of the main techniques currently in use,” Ann. Ig., vol. 25, pp. 31-42, 2013.
[31]	Y. F. Missirlis and A. D. Spiliotis, “Assessment of techniques used in calculating cell-material interactions,” Biomol. Eng., vol. 19, pp. 287-294, 2002.
[32]	R. B. Dickinson, J. A. Nagel, R. A. Proctor, and S. L. Cooper, “Quantitative comparison of shear-dependent staphylococcus aureus adhesion to three polyurethane ionomer analogs with distinct surface properties,” J. Biomed. Mater. Res., vol. 36, pp. 152-162, 1997.
[33]	P. de Jong, M. C. te Giffel, and E. A. Kiezebrink, “Prediction of the adherence, growth and release of microorganisms in production chains,” Int. J. Food Microbiol., vol. 74, pp. 13-25, 2002.
[34]	G. Binnig, C. F. Quate, and C. Gerber, “Atomic force microscope,” Phys. Rev. Lett., vol. 56, pp. 930-933, 1986.
[35]	D. Passeri, A. Bettucci, and M. Rossi, “Acoustic and atomic force microscopy for the mechanical characterization of thin films,” Anal. Bioanal. Chem., vol. 396, pp. 2769-2783, 2010.
[36]	D. Passeri, M. Rossi, E. Tamburri, and M. L. Terranova, “Mechanical characterization of polymeric thin films by atomic force microscopy based techniques,” Anal. Bioanal. Chem., vol. 405, pp. 1463-1478, 2013.
[37]	M. J. Cadena, R. Misiego, K. C. Smith, A. Avila, B. Pipes, R. Reifenberger, and A. Raman, “Sub-surface imaging of carbon nanotubepolymer composites using dynamic AFM methods,” Nanotechnology, vol. 24, p. 135706, 2013.
[38]	T. S. Jespersen and J. Nygård, “Mapping of individual carbon nanotubes in polymer/nanotube composites using electrostatic force microscopy,” Appl. Phys. Lett., vol. 90, p. 183108, 2007.
[39]	G. Fisichella, G. Greco, P. Fiorenza, S. Di Franco, F. Roccaforte, and F. Giannazzo, “Current mapping in graphene contacts to AlGaN/GaN heterostructures,” Nanoscience and Nanometrology, vol. 1, pp. 1-7, 2015.
[40]	U. Hartmann, “Magnetic force microscopy, “Annu. Rev. Mater. Sci., vol. 29, pp. 53-87, 1999.
[41]	F. Pillet, L. Chopinet, C. Formosa, and Étienne Dague, “Atomic force microscopy and pharmacology: From microbiology to cancerology,” BBA - Gen. Subjects, vol. 1840, pp. 1028-1050, 2014.
[42]	Y. F. Dufrêne, “Atomic force microscopy in microbiology: new structural and functional insights into the microbial cell surface, “mBio, vol. 22, pp. e01363-14, 2014.
[43]	D. Robichon, J.-C. Girard, Y. Cenatiempo, and J.-F. Cavellier, “Atomic force microscopy imaging of dried or living bacteria,” C. R. Acad. Sci. III-Vie, vol. 322, pp. 687-693, 1999.
[44]	K.-C. Chang, S.-J. Cheng, Y.-C. Chen, H.-R. Huang, and J.-W. Liou, “Nanoscopic analysis on pH induced morphological changes of flagella in Escherichia coli, “ J. Microbiol. Immunol., vol. 46, pp. 405-412, 2013.
[45]	P. Schaer-Zammaretti and J. Ubbink, “Imaging of lactic acid bacteria with AFM-elasticity and adhesion maps and their relationship to biological and structural data,” Ultramicroscopy, vol. 97, pp. 199-208, 2003.
[46]	A. Cerf, J.-C. Cau, C. Vieu, and E. Dague, “Nanomechanical properties of dead or alive single-patterned bacteria,” Langmuir, vol. 25, pp. 5731-5736, 2009.
[47]	A. Ebert, B. R. Tittmann, J. Du, and W. Scheuchenzuber, “Technique for rapid in vitro single-cell elastography, “ Ultrasound Med. Biol., vol. 32, pp. 1687-1702, 2006.
[48]	S. S. Nair, S. Wanga, and D. C. Hurley, “Nanoscale characterization of natural fibers and their composites using contact-resonance force microscopy,” Compos. Part A - Appl. S., vol. 41, pp. 624-631, 2010.
[49]	S. E. Campbell, V. L. Ferguson, and D. C. Hurley, “Nanomechanical mapping of the osteochondral interface with contact resonance force microscopy and nanoindentation,” Acta Biomater., vol. 8, pp. 4389-4396, 2012.
[50]	B. Cappella and G. Dietler, “Force-distance curves by atomic force microscopy,” Surf. Sci. Rep., vol. 34, pp. 1-104, 1999.
[51]	C. A. Clifford and M. P. Seah, “Quantification issues in the identification of nanoscale regions of homopolymers using modulus measurement via AFM nanoindentation,” Appl. Surf. Sci., vol. 252, pp. 1915-1933, 2005.
[52]	E. Tomasetti, R. Legras, and B. Nysten, “Quantitative approach towards the measurement of polypropylene/(ethylene-propylene) copolymer blends surface elastic properties by AFM,” Nanotechnology, vol. 9, pp. 305-315, 1998.
[53]	C. Reynaud, F. Sommer, C. Quet, N. El Buonia, and D. Tran Minh, “Quantitative determination of Young’s modulus on a biphase polymer system using atomic force microscopy,” Surf. Interface Anal., vol. 30, pp. 185-189, 2000.
[54]	D. Passeri, A. Bettucci, A. Biagioni, M. Rossi, A. Alippi, M. Lucci, I. Davoli, and S. Berezina, “Quantitative measurement of indentation hardness and modulus of compliant materials by atomic force microscopy,” Rev. Sci. Instrum., vol. 79, p. 066105, 2008.
[55]	D. Passeri, A. Bettucci, A. Biagioni, M. Rossi, A. Alippi, E. Tamburri, M. Lucci, I. Davoli, and S. Berezina, “Indentation modulus and hardness of viscoelastic thin films by atomic force microscopy: A case study,” Ultramicroscopy, vol. 109, pp. 1417-1427, 2009.
[56]	D. Passeri, A. Alippi, A. Bettucci, M. Rossi, A. Alippi, E. Tamburri, and M. L. Terranova, “Indentation modulus and hardness of polyaniline thin films by atomic force microscopy,” Synth. Met., vol. 161, pp. 7-12, 2011.
[57]	R. Jones, H. M. Pollock, J. A. S. Cleaver, and C. S. Hodges, “Adhesion forces between glass and silicon surfaces in air studied by AFM: Effects of relative humidity, particle size, roughness, and surface treatment,” Langmuir, vol. 18, pp. 8045-8055, 2002.
[58]	W. A. Ducker, T. J. Senden, and R. M. Pashley, “Direct measurement of colloidal forces using an atomic force microscope,” Nature, vol. 353, pp. 239-241, 1991.
[59]	A. Noy, “Chemical force microscopy of chemical and biological interactions,” Surf. Interface Anal., vol. 38, pp. 1429-1441, 2006.
[60]	D. V. Vezenov, A. Noy, and P. Ashby, “Chemical force microscopy: probing chemical origin of interfacial forces and adhesion,” J. Adhesion Sci. Technol., vol. 19, pp. 313- 364, 2005.
[61]	F. Kienberger, A. Ebner, H. J. Gruber, and P. Hinterdorfer, “Molecular recognition imaging and force spectroscopy of single biomolecules,” Acc. Chem. Res., vol. 39, pp. 29-36, 2006.
[62]	V. Dupres, C. Verbelen, and Y. F. Dufrene, “Probing molecular recognition sites on biosurfaces using AFM,” Biomaterials, vol. 28, pp. 2393-2402, 2007.
[63]	R. Creasey, S. Sharma, C. T. Gibson, J. E. Craig, A. Ebner, T. Becker, P. Hinterdorfer, and N. H. Voelcker, “Atomic force microscopy-based antibody recognition imaging of proteins in the pathological deposits in pseudoexfoliation syndrome,” Ultramicroscopy, vol. 111, pp. 1055-1061, 2011.
[64]	J. Zlatanova, S. M. Lindsay, and S. H. Leuba, “Single molecule force spectroscopy in biology using the atomic force microscope,” Prog. Bio- phys. Mol. Bio., vol. 74, pp. 37-61, 2000.
[65]	Y. Liu, J. Strauss, and T. A. Camesano, “Adhesion forces between staphylococcus epidermidis and surfaces bearing self-assembled monolayers in the presence of model proteins,” Biomaterials, vol. 29, pp. 4374-4382, 2008.
[66]	N. P. Boks, H. J. Busscher, H. C. van der Mei, and W. Norde, “Bond-strengthening in staphylococcal adhesion to hydrophilic and hydrophobic surfaces using atomic force microscopy,” Langmuir, vol. 24, pp. 12990-12994, 2008.
[67]	Y. Liu, P. A. Pinzón-Arango, A. M. Gallardo-Moreno, and T. A. Camesano, “Direct adhesion force measurements between e. coli and human uroepithelial cells in cranberry juice cocktail,” Mol. Nutr. Food Res., vol. 54, pp. 1744-1752, 2010.
[68]	P. Loskill, H. Hähl, N. Thewes, C. T. Kreis, M. Bischoff, M. Herrmann, and K. Jacobs, “Influence of the subsurface composition of a material on the adhesion of staphylococci,” Langmuir, vol. 28, pp. 7242-7248, 2012.
[69]	H. Xu, A. E. Murdaugh, W. Chen, K. E. Aidala, M. A. Ferguson, E. M. Spain, and M. E. Núñez, “Characterizing pilus-mediated adhesion of biofilm-forming E. coli to chemically diverse surfaces using atomic force microscopy,” Langmuir, vol. 29, pp. 3000-3011, 2013.
[70]	X. Sheng, Y. P. Ting, and S. O. Pehkonen, “Force measurements of bacterial adhesion on metals using a cell probe atomic force microscope,” J. Colloid Interface Sci., vol. 310, pp. 661-669, 2007.
[71]	X. Sheng, Y. P. Ting, and S. O. Pehkonen, “The influence of ionic strength, nutrients and pH on bacterial adhesion to metals,” J. Colloid Interface Sci., vol. 321, pp. 256-264, 2008.
[72]	D. T. L. Le, Y. Guérardel, P. Loubière, M. Mercier-Bonin, and E. Dague, “Measuring kinetic dissociation/association constants between lactococcus lactis bacteria and mucins using living cell probes,” Biophys. J., vol. 101, pp. 2843-2853, 2011.
[73]	J. Zhu, Q. Li, W. Jiao, H. Jiang, W. Sand, J. Xia, X. Liu, W. Qin, G. Qiu, Y. Hu, and L. Chai, “Adhesion forces between cells of acidithiobacillus ferrooxidans, acidithiobacillus thiooxidans or leptospirillum ferrooxidans and chalcopyrite,” Colloid. Surface. B, vol. 94, pp. 95-100, 2012.
[74]	J. Zhu, Q. Wang, S. Zhou, Q. Li, M. Gan, H. Jiang, W. Qin, X. Liu, Y. Hu, and G. Qiu, “Insights into the relation between adhesion force and chalcopyrite-bioleaching by acidithiobacillus ferrooxidans,” Colloid. Surface. B, vol. 126, pp. 351-357, 2015.
[75]	Y.-L. Ong, A. Razatos, G. Georgiou, and M. M. Sharma, “Adhesion forces between E. coli bacteria and biomaterial surfaces,” Langmuir, vol. 15, pp. 2719-2725, 1999.
[76]	E. L. Adams, S. Almagro-Moreno, and E. F. Boyd, “An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosides,” J. Microbiol. Meth., vol. 84, pp. 352-354, 2011.
[77]	W. R. Bowen, R. W. Lovitt, and C. J. Wright, “Atomic force microscopy study of the adhesion of saccharomyces cerevisiae,” J. Colloid Interface Sci., vol. 237, pp. 54-61, 2001.
[78]	V. Vadillo-Rodríguez, H. J. Busscher, W. Norde, J. de Vries, R. J. B. Dijkstra, I. Stokroos, and H. C. van der Mei, “Comparison of atomic force microscopy interaction forces between bacteria and siliconnitride substrata for three commonly used immobilization methods,” Appl. Environ. Microbiol., vol. 70, pp. 5441-5446, 2004.
[79]	S. Kang and M. Elimelech, “Bioinspired single bacterial cell force spectroscopy,” Langmuir, vol. 25, pp. 9656-9659, 2009.
[80]	G. Zeng, T. Müller, and R. L. Meyer, “Single-cell force spectroscopy of bacteria enabled by naturally derived proteins,” Langmuir, vol. 30, pp. 4019-4025, 2014.
[81]	W. Zhang, A. G. Stack, and Y. Chen, “Interaction force measurement between E. coli cells and nanoparticles immobilized surfaces by using AFM,” Colloid. Surface. B, vol. 82, pp. 316-324, 2011.
[82]	P. Loskill, C. Zeitz, S. Grandthyll, N. Thewes, F. Müller, M. Bischoff, M. Herrmann, and K. Jacobs, “Reduced adhesion of oral bacteria on hydroxyapatite by fluoride treatment,” Langmuir, vol. 29, pp. 5528-5533, 2013.
[83]	J. M. Thwala, M. Li, M. C. Y. Wong, S. Kang, E. M. V. Hoek, and B. B. Mamba, “Bacteria-polymeric membrane interactions: Atomic force microscopy and XDLVO predictions,” Langmuir, vol. 29, pp. 13773-13782, 2013.
[84]	A. Harimawan, S. Zhong, C.-T. Lim, and Y.-P. Ting, “Adhesion of B. subtilis spores and vegetative cells onto stainless steel - DLVO theories and AFM spectroscopy,” J. Colloid Interf. Sci, vol. 405, pp. 233-241, 2013.
[85]	A. Kumar and Y.-P. Ting, “Effect of sub-inhibitory antibacterial stress on bacterial surface properties and biofilm formation,” Colloid. Surface. B, vol. 111, pp. 747-754, 2013.
[86]	M. Diao, E. Taran, S. Mahler, T. A. H. Nguyen, and A. V. Nguyen, “Quantifying adhesion of acidophilic bioleaching bacteria to silica and pyrite by atomic force microscopy with a bacterial probe,” Colloid. Surface. B, vol. 115, pp. 229-236, 2014.
[87]	P. C. Y. Lau, J. R. Dutcher, T. J. Beveridge, and J. S. Lam, “Absolute quantification of bacterial biofilm adhesion and viscoelasticity by microbead force spectroscopy,” Biophys. J., vol. 96, pp. 2935-2948, 2009.
[88]	A. Beaussart, S. El-Kirat-Chatel, P. Herman, D. Alsteens, J. Mahillon, P. Hols, and Y. Dufrêne, “Single-cell force spectroscopy of probiotic bacteria,” Biophys. J., vol. 104, pp. 1886-1892, 2013.
[89]	S. K. Lower, C. J. Tadanier, and M. F. Hochella Jr., “Measuring interfacial and adhesion forces between bacteria and mineral surfaces with biological force microscopy,” Geochim. Cosmochim. Ac., vol. 64, pp. 3133-3139, 2000.
[90]	S. K. Lower, M. F. Hochella Jr., and T. J. Beveridge, “Bacterial recognition of mineral surfaces: Nanoscale interactions between Shewanella and α-feooh,” Science, vol. 292, pp. 1360-1363, 2001.
[91]	A. Meister, M. Gabi, P. Behr, P. Studer, J. Vörös, P. Niedermann, J. Bitterli, J. Polesel-Maris, M. Liley, H. Heinzelmann, and T. Zambelli, “FluidFM: Combining atomic force microscopy and nanofluidics in a universal liquid delivery system for single cell applications and beyond,” Nano Lett., vol. 9, pp. 2501-2507, 2009.
[92]	E. Potthoff, D. Ossola, T. Zambelli, and J. A. Vorholt, “Bacterial adhesion force quantification by fluidic force microscopy,” Nanoscale, vol. 7, pp. 4070-4079, 2015.
[93]	P. G. de Gennes, “Polymers at an interface; a simplified view,” Adv. Colloid Interfac., vol. 27, pp. 189-209, 1987.
[94]	X. L. Hu, B. Ho, C. T. Lim, and C. S. Hsu, “Thermal treatments modulate bacterial adhesion to dental enamel,” J. Dent. Res., vol. 90, pp. 1451-1456, 2011.

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Livia Angeloni, Daniele Passeri, Melania Reggente, Fabrizio Pantanella, Diego Mantovani, et al. (2015). Microbial Cells Force Spectroscopy by Atomic Force Microscopy: A Review. Nanoscience and Nanometrology, 2(1), 30-40. https://doi.org/10.11648/j.nsnm.20160201.13

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Livia Angeloni; Daniele Passeri; Melania Reggente; Fabrizio Pantanella; Diego Mantovani, et al. Microbial Cells Force Spectroscopy by Atomic Force Microscopy: A Review. Nanosci. Nanometrol. 2015, 2(1), 30-40. doi: 10.11648/j.nsnm.20160201.13

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Livia Angeloni, Daniele Passeri, Melania Reggente, Fabrizio Pantanella, Diego Mantovani, et al. Microbial Cells Force Spectroscopy by Atomic Force Microscopy: A Review. Nanosci Nanometrol. 2015;2(1):30-40. doi: 10.11648/j.nsnm.20160201.13

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@article{10.11648/j.nsnm.20160201.13,
  author = {Livia Angeloni and Daniele Passeri and Melania Reggente and Fabrizio Pantanella and Diego Mantovani and Marco Rossi},
  title = {Microbial Cells Force Spectroscopy by Atomic Force Microscopy: A Review},
  journal = {Nanoscience and Nanometrology},
  volume = {2},
  number = {1},
  pages = {30-40},
  doi = {10.11648/j.nsnm.20160201.13},
  url = {https://doi.org/10.11648/j.nsnm.20160201.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.nsnm.20160201.13},
  abstract = {Bacterial adhesion and biofilm formation are important phenomena which can produce both detrimental and beneficial effects in several fields. Research is thus focused on the modulation of the properties of material surfaces in order to design and develop substrates able to control bacterial adhesion process, which is the first trigger event of biofilm formation. Several theoretical predictions and experimental procedures have been developed to investigate the physical, chemical and biological mechanisms regulating the attachment of bacteria to solid substrates. Nevertheless, a comprehensive understanding has not been achieved yet, limiting the capability of individuating effective technological strategies to achieve the desired bacterial adhesion behavior. The development of new experimental procedures able to furnish deeper information about bacterial adhesion mechanism is thus needed. Microbial cell force spectroscopy (MCFS) is an atomic force microscopy (AFM) based technique, consisting in the detection of force-distance curves using particular probes obtained immobilizing bacterial cells at the free end of a flexible microcantilever, which allows the detection of the different kinds of cell-surface interaction forces. In this work, we review the state of the art in the development of MCFS, focusing on its working principle and applications. A brief description of the current models and conventional experimental procedures used to evaluate bacterial adhesion to surfaces is reported. Then, the instrumentation and the working principle, the current procedures used to prepare bacterial cells probes and the main applications of the technique are described with the aim of pointing out the advantages of the technique and the limits which still have to be overcome.},
 year = {2015}
}

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TY  - JOUR
T1  - Microbial Cells Force Spectroscopy by Atomic Force Microscopy: A Review
AU  - Livia Angeloni
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AU  - Melania Reggente
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SN  - 2472-3630
UR  - https://doi.org/10.11648/j.nsnm.20160201.13
AB  - Bacterial adhesion and biofilm formation are important phenomena which can produce both detrimental and beneficial effects in several fields. Research is thus focused on the modulation of the properties of material surfaces in order to design and develop substrates able to control bacterial adhesion process, which is the first trigger event of biofilm formation. Several theoretical predictions and experimental procedures have been developed to investigate the physical, chemical and biological mechanisms regulating the attachment of bacteria to solid substrates. Nevertheless, a comprehensive understanding has not been achieved yet, limiting the capability of individuating effective technological strategies to achieve the desired bacterial adhesion behavior. The development of new experimental procedures able to furnish deeper information about bacterial adhesion mechanism is thus needed. Microbial cell force spectroscopy (MCFS) is an atomic force microscopy (AFM) based technique, consisting in the detection of force-distance curves using particular probes obtained immobilizing bacterial cells at the free end of a flexible microcantilever, which allows the detection of the different kinds of cell-surface interaction forces. In this work, we review the state of the art in the development of MCFS, focusing on its working principle and applications. A brief description of the current models and conventional experimental procedures used to evaluate bacterial adhesion to surfaces is reported. Then, the instrumentation and the working principle, the current procedures used to prepare bacterial cells probes and the main applications of the technique are described with the aim of pointing out the advantages of the technique and the limits which still have to be overcome.
VL  - 2
IS  - 1
ER  -

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Author Information

Livia Angeloni

Department of Basic and Applied Sciences for Engineering, Sapienza University of Rome, Rome, Italy
Daniele Passeri

Department of Basic and Applied Sciences for Engineering, Sapienza University of Rome, Rome, Italy
Melania Reggente

Department of Basic and Applied Sciences for Engineering, Sapienza University of Rome, Rome, Italy
Fabrizio Pantanella

Department of Public Health and Infectious Diseases, SAPIENZA University of Rome, Rome, Italy
Diego Mantovani

Lab. for Biomaterials and Bioengineering (CRC-I), Dept. Min-Met-Materials Eng. & University Hospital Research Center, Laval University, Quebec City, Canada
Marco Rossi

Department of Basic and Applied Sciences for Engineering, Sapienza University of Rome, Rome, Italy

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APA Style

Livia Angeloni, Daniele Passeri, Melania Reggente, Fabrizio Pantanella, Diego Mantovani, et al. (2015). Microbial Cells Force Spectroscopy by Atomic Force Microscopy: A Review. Nanoscience and Nanometrology, 2(1), 30-40. https://doi.org/10.11648/j.nsnm.20160201.13

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ACS Style

Livia Angeloni; Daniele Passeri; Melania Reggente; Fabrizio Pantanella; Diego Mantovani, et al. Microbial Cells Force Spectroscopy by Atomic Force Microscopy: A Review. Nanosci. Nanometrol. 2015, 2(1), 30-40. doi: 10.11648/j.nsnm.20160201.13

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AMA Style

Livia Angeloni, Daniele Passeri, Melania Reggente, Fabrizio Pantanella, Diego Mantovani, et al. Microbial Cells Force Spectroscopy by Atomic Force Microscopy: A Review. Nanosci Nanometrol. 2015;2(1):30-40. doi: 10.11648/j.nsnm.20160201.13

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@article{10.11648/j.nsnm.20160201.13,
  author = {Livia Angeloni and Daniele Passeri and Melania Reggente and Fabrizio Pantanella and Diego Mantovani and Marco Rossi},
  title = {Microbial Cells Force Spectroscopy by Atomic Force Microscopy: A Review},
  journal = {Nanoscience and Nanometrology},
  volume = {2},
  number = {1},
  pages = {30-40},
  doi = {10.11648/j.nsnm.20160201.13},
  url = {https://doi.org/10.11648/j.nsnm.20160201.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.nsnm.20160201.13},
  abstract = {Bacterial adhesion and biofilm formation are important phenomena which can produce both detrimental and beneficial effects in several fields. Research is thus focused on the modulation of the properties of material surfaces in order to design and develop substrates able to control bacterial adhesion process, which is the first trigger event of biofilm formation. Several theoretical predictions and experimental procedures have been developed to investigate the physical, chemical and biological mechanisms regulating the attachment of bacteria to solid substrates. Nevertheless, a comprehensive understanding has not been achieved yet, limiting the capability of individuating effective technological strategies to achieve the desired bacterial adhesion behavior. The development of new experimental procedures able to furnish deeper information about bacterial adhesion mechanism is thus needed. Microbial cell force spectroscopy (MCFS) is an atomic force microscopy (AFM) based technique, consisting in the detection of force-distance curves using particular probes obtained immobilizing bacterial cells at the free end of a flexible microcantilever, which allows the detection of the different kinds of cell-surface interaction forces. In this work, we review the state of the art in the development of MCFS, focusing on its working principle and applications. A brief description of the current models and conventional experimental procedures used to evaluate bacterial adhesion to surfaces is reported. Then, the instrumentation and the working principle, the current procedures used to prepare bacterial cells probes and the main applications of the technique are described with the aim of pointing out the advantages of the technique and the limits which still have to be overcome.},
 year = {2015}
}

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TY  - JOUR
T1  - Microbial Cells Force Spectroscopy by Atomic Force Microscopy: A Review
AU  - Livia Angeloni
AU  - Daniele Passeri
AU  - Melania Reggente
AU  - Fabrizio Pantanella
AU  - Diego Mantovani
AU  - Marco Rossi
Y1  - 2015/10/15
PY  - 2015
N1  - https://doi.org/10.11648/j.nsnm.20160201.13
DO  - 10.11648/j.nsnm.20160201.13
T2  - Nanoscience and Nanometrology
JF  - Nanoscience and Nanometrology
JO  - Nanoscience and Nanometrology
SP  - 30
EP  - 40
PB  - Science Publishing Group
SN  - 2472-3630
UR  - https://doi.org/10.11648/j.nsnm.20160201.13
AB  - Bacterial adhesion and biofilm formation are important phenomena which can produce both detrimental and beneficial effects in several fields. Research is thus focused on the modulation of the properties of material surfaces in order to design and develop substrates able to control bacterial adhesion process, which is the first trigger event of biofilm formation. Several theoretical predictions and experimental procedures have been developed to investigate the physical, chemical and biological mechanisms regulating the attachment of bacteria to solid substrates. Nevertheless, a comprehensive understanding has not been achieved yet, limiting the capability of individuating effective technological strategies to achieve the desired bacterial adhesion behavior. The development of new experimental procedures able to furnish deeper information about bacterial adhesion mechanism is thus needed. Microbial cell force spectroscopy (MCFS) is an atomic force microscopy (AFM) based technique, consisting in the detection of force-distance curves using particular probes obtained immobilizing bacterial cells at the free end of a flexible microcantilever, which allows the detection of the different kinds of cell-surface interaction forces. In this work, we review the state of the art in the development of MCFS, focusing on its working principle and applications. A brief description of the current models and conventional experimental procedures used to evaluate bacterial adhesion to surfaces is reported. Then, the instrumentation and the working principle, the current procedures used to prepare bacterial cells probes and the main applications of the technique are described with the aim of pointing out the advantages of the technique and the limits which still have to be overcome.
VL  - 2
IS  - 1
ER  -

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