Rapid Screening of Barley Stripe Mosaic Virus Via Microfluidic Chip Method

Wang Jiaying; Cui Junxia; Li Wen; Duan Weijun

doi:doi:10.11648/j.jps.20231101.12

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Rapid Screening of Barley Stripe Mosaic Virus Via Microfluidic Chip Method

Wang Jiaying, Cui Junxia, Li Wen, Duan Weijun

Published in Journal of Plant Sciences (Volume 11, Issue 1)

Received: 4 January 2023 Accepted: 29 January 2023 Published: 14 February 2023

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Abstract

Barley stripe mosaic virus, the type member of Hordeivirus, constitutes an important seed-transmitted pathogen distributed in almost every barley growing area worldwide. Researches on efficient detection of BSMV suitable in wild application are in urgent need. New platforms like microfluidic technology keep progressing rapidly in past decades and gains increasing influence on life sciences. Thus in this study, one rapid screening method for BSMV via microfluidic chip was established. Through method validity, we succeeded in demonstrating the amplification of BSMV within 30 min at one constant temperature using a microfluidic chip assay. Sensitivity analysis conformed that this method obtained a detection limit at 1.00×102 copies/μL, which was even more sensitive than real-time RT-PCR. Maize chlorotic mottle virus, Maize dwarf mosaic virus, Oat mosaic virus, and Wheat streak mosaic virus were chosen in specificity test along with BSMV, but only BSMV expressed typical amplification curves and Ct. Microfluidic analytic systems realize miniaturization by reducing the reaction volume to just 5 μL, which has several superiorities such as saving expensive reagents and reducing inspection costs. Moreover this microfluidic chip assay can simultaneously detect a variety of viruses, and the whole process takes just 1 hour, which greatly speeds up the detection and improves efficiency. All in all, this microfluidic chip method has the potential to be further implemented by phytosanitary services for routine diagnose as well as rapid screening in places like ports.

Published in	Journal of Plant Sciences (Volume 11, Issue 1)
DOI	10.11648/j.jps.20231101.12
Page(s)	9-16
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Barley Stripe Mosaic Virus, Microfluidic Chip, Routine Diagnose, Rapid Screening, Disease Control

References

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[18]	Xie L, Zhang JZ, Wang Q, et al. Characterization of Maize Chlorotic Mottle Virus Associated with Maize Lethal Necrosis Disease in China. Journal of Phytopathology. 2011, 159 (3): 191-193.
[19]	Kannan M, Ismail I, Bunawan H. Maize dwarf mosaic virus: From genome to disease management. Viruses. 2018, 10 (9): 492.
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APA Style

Wang Jiaying, Cui Junxia, Li Wen, Duan Weijun. (2023). Rapid Screening of Barley Stripe Mosaic Virus Via Microfluidic Chip Method. Journal of Plant Sciences, 11(1), 9-16. https://doi.org/10.11648/j.jps.20231101.12

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ACS Style

Wang Jiaying; Cui Junxia; Li Wen; Duan Weijun. Rapid Screening of Barley Stripe Mosaic Virus Via Microfluidic Chip Method. J. Plant Sci. 2023, 11(1), 9-16. doi: 10.11648/j.jps.20231101.12

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AMA Style

Wang Jiaying, Cui Junxia, Li Wen, Duan Weijun. Rapid Screening of Barley Stripe Mosaic Virus Via Microfluidic Chip Method. J Plant Sci. 2023;11(1):9-16. doi: 10.11648/j.jps.20231101.12

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@article{10.11648/j.jps.20231101.12,
  author = {Wang Jiaying and Cui Junxia and Li Wen and Duan Weijun},
  title = {Rapid Screening of Barley Stripe Mosaic Virus Via Microfluidic Chip Method},
  journal = {Journal of Plant Sciences},
  volume = {11},
  number = {1},
  pages = {9-16},
  doi = {10.11648/j.jps.20231101.12},
  url = {https://doi.org/10.11648/j.jps.20231101.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20231101.12},
  abstract = {Barley stripe mosaic virus, the type member of Hordeivirus, constitutes an important seed-transmitted pathogen distributed in almost every barley growing area worldwide. Researches on efficient detection of BSMV suitable in wild application are in urgent need. New platforms like microfluidic technology keep progressing rapidly in past decades and gains increasing influence on life sciences. Thus in this study, one rapid screening method for BSMV via microfluidic chip was established. Through method validity, we succeeded in demonstrating the amplification of BSMV within 30 min at one constant temperature using a microfluidic chip assay. Sensitivity analysis conformed that this method obtained a detection limit at 1.00×102 copies/μL, which was even more sensitive than real-time RT-PCR. Maize chlorotic mottle virus, Maize dwarf mosaic virus, Oat mosaic virus, and Wheat streak mosaic virus were chosen in specificity test along with BSMV, but only BSMV expressed typical amplification curves and Ct. Microfluidic analytic systems realize miniaturization by reducing the reaction volume to just 5 μL, which has several superiorities such as saving expensive reagents and reducing inspection costs. Moreover this microfluidic chip assay can simultaneously detect a variety of viruses, and the whole process takes just 1 hour, which greatly speeds up the detection and improves efficiency. All in all, this microfluidic chip method has the potential to be further implemented by phytosanitary services for routine diagnose as well as rapid screening in places like ports.},
 year = {2023}
}

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TY  - JOUR
T1  - Rapid Screening of Barley Stripe Mosaic Virus Via Microfluidic Chip Method
AU  - Wang Jiaying
AU  - Cui Junxia
AU  - Li Wen
AU  - Duan Weijun
Y1  - 2023/02/14
PY  - 2023
N1  - https://doi.org/10.11648/j.jps.20231101.12
DO  - 10.11648/j.jps.20231101.12
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 9
EP  - 16
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20231101.12
AB  - Barley stripe mosaic virus, the type member of Hordeivirus, constitutes an important seed-transmitted pathogen distributed in almost every barley growing area worldwide. Researches on efficient detection of BSMV suitable in wild application are in urgent need. New platforms like microfluidic technology keep progressing rapidly in past decades and gains increasing influence on life sciences. Thus in this study, one rapid screening method for BSMV via microfluidic chip was established. Through method validity, we succeeded in demonstrating the amplification of BSMV within 30 min at one constant temperature using a microfluidic chip assay. Sensitivity analysis conformed that this method obtained a detection limit at 1.00×102 copies/μL, which was even more sensitive than real-time RT-PCR. Maize chlorotic mottle virus, Maize dwarf mosaic virus, Oat mosaic virus, and Wheat streak mosaic virus were chosen in specificity test along with BSMV, but only BSMV expressed typical amplification curves and Ct. Microfluidic analytic systems realize miniaturization by reducing the reaction volume to just 5 μL, which has several superiorities such as saving expensive reagents and reducing inspection costs. Moreover this microfluidic chip assay can simultaneously detect a variety of viruses, and the whole process takes just 1 hour, which greatly speeds up the detection and improves efficiency. All in all, this microfluidic chip method has the potential to be further implemented by phytosanitary services for routine diagnose as well as rapid screening in places like ports.
VL  - 11
IS  - 1
ER  -

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Author Information

Wang Jiaying

Technical Center, Ningbo Customs, Ningbo, China
Cui Junxia

Technical Center, Ningbo Customs, Ningbo, China
Li Wen

Department of Horticultural Technology, Ningbo City College of Vocational Technology, Ningbo, China
Duan Weijun

Technical Center, Ningbo Customs, Ningbo, China

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Plain Text BibTeX RIS

APA Style

Wang Jiaying, Cui Junxia, Li Wen, Duan Weijun. (2023). Rapid Screening of Barley Stripe Mosaic Virus Via Microfluidic Chip Method. Journal of Plant Sciences, 11(1), 9-16. https://doi.org/10.11648/j.jps.20231101.12

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ACS Style

Wang Jiaying; Cui Junxia; Li Wen; Duan Weijun. Rapid Screening of Barley Stripe Mosaic Virus Via Microfluidic Chip Method. J. Plant Sci. 2023, 11(1), 9-16. doi: 10.11648/j.jps.20231101.12

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AMA Style

Wang Jiaying, Cui Junxia, Li Wen, Duan Weijun. Rapid Screening of Barley Stripe Mosaic Virus Via Microfluidic Chip Method. J Plant Sci. 2023;11(1):9-16. doi: 10.11648/j.jps.20231101.12

Copy | Download

@article{10.11648/j.jps.20231101.12,
  author = {Wang Jiaying and Cui Junxia and Li Wen and Duan Weijun},
  title = {Rapid Screening of Barley Stripe Mosaic Virus Via Microfluidic Chip Method},
  journal = {Journal of Plant Sciences},
  volume = {11},
  number = {1},
  pages = {9-16},
  doi = {10.11648/j.jps.20231101.12},
  url = {https://doi.org/10.11648/j.jps.20231101.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20231101.12},
  abstract = {Barley stripe mosaic virus, the type member of Hordeivirus, constitutes an important seed-transmitted pathogen distributed in almost every barley growing area worldwide. Researches on efficient detection of BSMV suitable in wild application are in urgent need. New platforms like microfluidic technology keep progressing rapidly in past decades and gains increasing influence on life sciences. Thus in this study, one rapid screening method for BSMV via microfluidic chip was established. Through method validity, we succeeded in demonstrating the amplification of BSMV within 30 min at one constant temperature using a microfluidic chip assay. Sensitivity analysis conformed that this method obtained a detection limit at 1.00×102 copies/μL, which was even more sensitive than real-time RT-PCR. Maize chlorotic mottle virus, Maize dwarf mosaic virus, Oat mosaic virus, and Wheat streak mosaic virus were chosen in specificity test along with BSMV, but only BSMV expressed typical amplification curves and Ct. Microfluidic analytic systems realize miniaturization by reducing the reaction volume to just 5 μL, which has several superiorities such as saving expensive reagents and reducing inspection costs. Moreover this microfluidic chip assay can simultaneously detect a variety of viruses, and the whole process takes just 1 hour, which greatly speeds up the detection and improves efficiency. All in all, this microfluidic chip method has the potential to be further implemented by phytosanitary services for routine diagnose as well as rapid screening in places like ports.},
 year = {2023}
}

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TY  - JOUR
T1  - Rapid Screening of Barley Stripe Mosaic Virus Via Microfluidic Chip Method
AU  - Wang Jiaying
AU  - Cui Junxia
AU  - Li Wen
AU  - Duan Weijun
Y1  - 2023/02/14
PY  - 2023
N1  - https://doi.org/10.11648/j.jps.20231101.12
DO  - 10.11648/j.jps.20231101.12
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 9
EP  - 16
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20231101.12
AB  - Barley stripe mosaic virus, the type member of Hordeivirus, constitutes an important seed-transmitted pathogen distributed in almost every barley growing area worldwide. Researches on efficient detection of BSMV suitable in wild application are in urgent need. New platforms like microfluidic technology keep progressing rapidly in past decades and gains increasing influence on life sciences. Thus in this study, one rapid screening method for BSMV via microfluidic chip was established. Through method validity, we succeeded in demonstrating the amplification of BSMV within 30 min at one constant temperature using a microfluidic chip assay. Sensitivity analysis conformed that this method obtained a detection limit at 1.00×102 copies/μL, which was even more sensitive than real-time RT-PCR. Maize chlorotic mottle virus, Maize dwarf mosaic virus, Oat mosaic virus, and Wheat streak mosaic virus were chosen in specificity test along with BSMV, but only BSMV expressed typical amplification curves and Ct. Microfluidic analytic systems realize miniaturization by reducing the reaction volume to just 5 μL, which has several superiorities such as saving expensive reagents and reducing inspection costs. Moreover this microfluidic chip assay can simultaneously detect a variety of viruses, and the whole process takes just 1 hour, which greatly speeds up the detection and improves efficiency. All in all, this microfluidic chip method has the potential to be further implemented by phytosanitary services for routine diagnose as well as rapid screening in places like ports.
VL  - 11
IS  - 1
ER  -

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