The purpose of this study, to find out the effect of GA3 and IAA on potato micro plants in vitro and further performance in net house and in field. This study was carried out between 2014-2015 in the Central Potato Research Institute Modipuram, Meerut. In this study used different concentrations of GA3 and IAA in combinations. For this experiment used 3 node stems cutting of potato variety Kufri Bahar and Kufri Surya with MS media supplemented with different concentrations and combinations of GA3+IAA (0.05 mg GA3/L+0.005 mg IAA/L, 0.1 mg GA3/L+0.01 mg IAA /L, 0.2 mg GA3/L+0.01 mg IAA /L, 0.1 mg GA3/L+0.02 mg IAA /L, 0.2 GA3 mg/L+0.02 mg IAA /L and 0.4 mgGA3/L+0.04 mg IAA/L) for microplant culture. Application of GA3+IAA at 0.4 mg/L+0.04 mg/L significantly increased length of shoot, number of nodes, internode length, number of leaves, length of root and fresh weight of shoot per plant in vitro. Similarly, the application of GA3+IAA at 0.4 mg/L+0.04 mg/L also increased the growth and yield parameters of potato variety Kufri Bahar and Kufri Surya in net house and in field. Potato quality and quantity can therefore be improved through application of 0.4 mgGA3/L+0.04 mg IAA/L.
Published in | Journal of Plant Sciences (Volume 10, Issue 6) |
DOI | 10.11648/j.jps.20221006.15 |
Page(s) | 227-234 |
Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
GA3, IAA, Microplants, Minitubers, In-Vitro, Field, Kufri Bahar, Kufri Surya
[1] | Birbal, B., Singh, R. K. and V. S. Kushwaha (2008). Effect of plant growth regulators on potato production. J. Indian Potato Assoc. 35: 387-391. |
[2] | El-Banna E. N., A. H. El-Morsy and M. R. Mohamad (2006). Impact of potato seed s trying by GA3 and IAA on growth tuber components and yields. J. Agric. Sci. Mansoura Uni. 31: 3869-3879. |
[3] | Farm sector news, Agricultural situation in India Vol. 78 (1) April 2021. |
[4] | Faten, S., Abd-el. A., Shaheen, A. M. and A. R. Fatma (2008). The effect of foliar application of GA3 and soil dressing of NPK at different levels on the plant productivity of potatoes (Solanum tuberosum L.). Research Journal of Agriculture and Biological Science 4: 384-391. |
[5] | Ghimire, N. P. (1986). Effect of different levels of plant growth regulators on various agronomic trait in okra (Abelmoschus esculentus L. Moench). Annals Applied Biology 5: 72-74. |
[6] | Hoque, W., Li, H., Liu, F. and L. Xiao (2009). Chloro choline chloride application effects on photosynthetic capacity and potato assimilates partitioning in potato (Solanum tuberosum L.). Scientia Horticul. 2: 20. |
[7] | Imma, F. And A. M. Mingo-Castel (2006). Potato minituber production using aeroponics: Effect of plant density and harvesting intervals. American Journal of Potato Research 83: 47-53. |
[8] | Khalid, A. F. Aftab (2020). Effect of exogenous application of IAA and GA 3 on growth, protein content, and antioxidant enzymes of Solanum tuberosum L. grown in vitro under salt stress. In Vitro Cellular & Developmental Biology-Plant, 1-13. https://doi.org/10.1007/s11627-019-10047-x |
[9] | Kondhare K. R, Aruna B. P., and P. G. Ashok (2021). auxin: an emerging regulator of tuber and storage root development. Plant Science 306: 1-10. |
[10] | Kumar, A., Singh, B. P. and H. Katiyar (2012). Effect of foliar application of plant growth regulators on potato tuber quality. Progressive Horticulture 44: 299-303. |
[11] | Kumar, P., Alka, Rao, P. and B. D. Baijal (1981). Effect of some growth regulators on plant growth, tuber initiation, yield and chemical composition of potato (Solanum tuberosum L.). Pak. J. Bot. 1: 69-75. |
[12] | Kumlay, A. M. (2014). Combination of the auxin NAA, IBA, and IAA with GA3 improves the commercial seed-tuber production of potato (Solanum tuberosum L.) under in vitro condition. Biomed. Research International 1: 1-7. |
[13] | Lim, O. T., Choi, S. P. and S. P. Dhital (2004). Application of gibberellic acid and paclobutrazol for efficient production of potato (Solanum tuberosum L.) minitubers and their dormancy breaking under soilless culture system. J. Kor. Soc. Hort. Sci. 45: 189-193. |
[14] | Murashige, T. and F. Skoog (1962). A revised medium for rapid growth and bio assays with tobacco tissue culture. Physiol Plantarum 15: 473-497. |
[15] | Novikov, O. O, Romanova, M. S., Leonov, N. I. and Kosinova, E. I. (2021). Influence of various phytohormones on the growth and development of the Solnechny potato variety in vitro. BIO Web of Conferences 36: 05008. https://doi.org/10.1051/bioconf/20213605008 |
[16] | Otroshy, M. (2006). Utilization of Tissue Culture Techniques in Seed Potato Tuber Production Scheme. Ph. D thesis, Wageningen University. |
[17] | Rahdari, P., Khosroabadi, M. and K. Delfani (2014). Effect of different concentration of plant hormones (IBA and NAA) on rooting and growth factor in root and stem cutting of Cordyline terminalis. Journal of Medical and Bioengineering 3: 190-194. |
[18] | Roumeliotis, E., Kloosterman, B., Oortwijn, M., Kohlen, W., Bouwmeester, H. J. and R. G. Visser (2012). The effect of auxin and strigolactones on tuber initiation and stolon architecture in potato. J. Exp. Bot. 63: 4539-4547. |
[19] | Struik P. C. and S. G. Wiersema (1999). Seed Potato Technology. Wageningen Press, Wageningen, The Netherlands. |
[20] | Venkatasalam, E. P., Latawa, J., Chakrabarti, S. K., Pandey, K. K., Sood, R., Thakur, V., Sharma, A. K. and B. P. Singh (2015). Standardisation of medium for micropropagation of recalcitrant potato (Solanum tuberosum L.) cultivar Kufri Jyoti. Potato J. 42: 116-123. |
[21] | Xhulaj, D., and B. Gixhari (2018). Agriculture and Forestry, 4, 105-112. |
[22] | Xu X, van Lammeren AAM, Vermeer E, Vreugdenhil D. 1998. The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiol. 117: 575–84. |
[23] | Zhang, Z., W. Zhou and H. Li. (2005). The role of GA, IAA and BAP in the regulation of in vitro shoot growth and microtuberization in potato. Acta Physiologiae Plantarum. 27: 363–369. |
[24] | Zierer W., Ruscher D., Sonneward U., and S. Sonnewald (2021). Annual Review of Plant Biology Tuber and Tuberous Root Development Annu. Rev. Plant Biol. 72: 26.1–26.30. |
APA Style
Meenakshi Kumari. (2022). Potato (Solanum tuberosum L.) Microplants and Minitubers Effected by the Combination of Gibberellic Acid (GA3) and Indole 3 Acetic Acid (IAA). Journal of Plant Sciences, 10(6), 227-234. https://doi.org/10.11648/j.jps.20221006.15
ACS Style
Meenakshi Kumari. Potato (Solanum tuberosum L.) Microplants and Minitubers Effected by the Combination of Gibberellic Acid (GA3) and Indole 3 Acetic Acid (IAA). J. Plant Sci. 2022, 10(6), 227-234. doi: 10.11648/j.jps.20221006.15
@article{10.11648/j.jps.20221006.15, author = {Meenakshi Kumari}, title = {Potato (Solanum tuberosum L.) Microplants and Minitubers Effected by the Combination of Gibberellic Acid (GA3) and Indole 3 Acetic Acid (IAA)}, journal = {Journal of Plant Sciences}, volume = {10}, number = {6}, pages = {227-234}, doi = {10.11648/j.jps.20221006.15}, url = {https://doi.org/10.11648/j.jps.20221006.15}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20221006.15}, abstract = {The purpose of this study, to find out the effect of GA3 and IAA on potato micro plants in vitro and further performance in net house and in field. This study was carried out between 2014-2015 in the Central Potato Research Institute Modipuram, Meerut. In this study used different concentrations of GA3 and IAA in combinations. For this experiment used 3 node stems cutting of potato variety Kufri Bahar and Kufri Surya with MS media supplemented with different concentrations and combinations of GA3+IAA (0.05 mg GA3/L+0.005 mg IAA/L, 0.1 mg GA3/L+0.01 mg IAA /L, 0.2 mg GA3/L+0.01 mg IAA /L, 0.1 mg GA3/L+0.02 mg IAA /L, 0.2 GA3 mg/L+0.02 mg IAA /L and 0.4 mgGA3/L+0.04 mg IAA/L) for microplant culture. Application of GA3+IAA at 0.4 mg/L+0.04 mg/L significantly increased length of shoot, number of nodes, internode length, number of leaves, length of root and fresh weight of shoot per plant in vitro. Similarly, the application of GA3+IAA at 0.4 mg/L+0.04 mg/L also increased the growth and yield parameters of potato variety Kufri Bahar and Kufri Surya in net house and in field. Potato quality and quantity can therefore be improved through application of 0.4 mgGA3/L+0.04 mg IAA/L.}, year = {2022} }
TY - JOUR T1 - Potato (Solanum tuberosum L.) Microplants and Minitubers Effected by the Combination of Gibberellic Acid (GA3) and Indole 3 Acetic Acid (IAA) AU - Meenakshi Kumari Y1 - 2022/11/30 PY - 2022 N1 - https://doi.org/10.11648/j.jps.20221006.15 DO - 10.11648/j.jps.20221006.15 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 227 EP - 234 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20221006.15 AB - The purpose of this study, to find out the effect of GA3 and IAA on potato micro plants in vitro and further performance in net house and in field. This study was carried out between 2014-2015 in the Central Potato Research Institute Modipuram, Meerut. In this study used different concentrations of GA3 and IAA in combinations. For this experiment used 3 node stems cutting of potato variety Kufri Bahar and Kufri Surya with MS media supplemented with different concentrations and combinations of GA3+IAA (0.05 mg GA3/L+0.005 mg IAA/L, 0.1 mg GA3/L+0.01 mg IAA /L, 0.2 mg GA3/L+0.01 mg IAA /L, 0.1 mg GA3/L+0.02 mg IAA /L, 0.2 GA3 mg/L+0.02 mg IAA /L and 0.4 mgGA3/L+0.04 mg IAA/L) for microplant culture. Application of GA3+IAA at 0.4 mg/L+0.04 mg/L significantly increased length of shoot, number of nodes, internode length, number of leaves, length of root and fresh weight of shoot per plant in vitro. Similarly, the application of GA3+IAA at 0.4 mg/L+0.04 mg/L also increased the growth and yield parameters of potato variety Kufri Bahar and Kufri Surya in net house and in field. Potato quality and quantity can therefore be improved through application of 0.4 mgGA3/L+0.04 mg IAA/L. VL - 10 IS - 6 ER -