Owing to the high pharmacological and economic value, the Chinese medicinal herbs: Paris polyphylla var. yunnanensis, Polygonatum kingianum and Bletilla striata, whose quality has been damaged by their adulterants. In this study, the allele-specific diagnostic PCR method was used to identify three herbal medicines and their adulterants. And the nuclear gene ITS2 sequence and chloroplast gene psbA-trnH sequence, as international common barcode sequences, are widely used in the molecular identification of medicinal plants. Therefore, three pair of specific PCR primers were respectively designed according to the sequence of Paris polyphylla var. yunnanensis on the ITS2, Polygonatum kingianum and Bletilla striata on the psbA-trnH, using Primer 5.0. And these specific identification primers could amplify special bands respectively at 130bp, 331bp and 508bp, while adulterants could not amplify the bands. The results showed that this method could respectively distinguish three herbal medicines and their adulterants. And after many experiments, the method had good repeatability. The allele-specific PCR identification method established in this study is easy to operate and fast in identification. This method can distinguish three kinds of traditional Chinese medicines and their adulterants, which provides a reference for the identification of other traditional Chinese medicines, so as to further protect the Chinese herbal medicine industry from economically motivated adulteration.
| Published in | Journal of Plant Sciences (Volume 10, Issue 6) |
| DOI | 10.11648/j.jps.20221006.13 |
| Page(s) | 214-221 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Paris Polyphylla var. Yunnanensis, Polygonatum Kingianum, Bletilla Striata, Adulterants, Allele-Specific Diagnostic PCR, Identification
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APA Style
Hao Chen, Xiao-Lei Chen, Jian-Yun Su, Yan-Ying Li, Xiao-Bin Hu, et al. (2022). Identification of Three Chinese Herbal Medicines and Their Adulterants Based on Allele-Specific Diagnostic PCR. Journal of Plant Sciences, 10(6), 214-221. https://doi.org/10.11648/j.jps.20221006.13
ACS Style
Hao Chen; Xiao-Lei Chen; Jian-Yun Su; Yan-Ying Li; Xiao-Bin Hu, et al. Identification of Three Chinese Herbal Medicines and Their Adulterants Based on Allele-Specific Diagnostic PCR. J. Plant Sci. 2022, 10(6), 214-221. doi: 10.11648/j.jps.20221006.13
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Download@article{10.11648/j.jps.20221006.13,
author = {Hao Chen and Xiao-Lei Chen and Jian-Yun Su and Yan-Ying Li and Xiao-Bin Hu and Zhe Xu and Hua-Li Qian and Lei Zhang and Jia-Hong Dong and Peng-Zhang Ji},
title = {Identification of Three Chinese Herbal Medicines and Their Adulterants Based on Allele-Specific Diagnostic PCR},
journal = {Journal of Plant Sciences},
volume = {10},
number = {6},
pages = {214-221},
doi = {10.11648/j.jps.20221006.13},
url = {https://doi.org/10.11648/j.jps.20221006.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20221006.13},
abstract = {Owing to the high pharmacological and economic value, the Chinese medicinal herbs: Paris polyphylla var. yunnanensis, Polygonatum kingianum and Bletilla striata, whose quality has been damaged by their adulterants. In this study, the allele-specific diagnostic PCR method was used to identify three herbal medicines and their adulterants. And the nuclear gene ITS2 sequence and chloroplast gene psbA-trnH sequence, as international common barcode sequences, are widely used in the molecular identification of medicinal plants. Therefore, three pair of specific PCR primers were respectively designed according to the sequence of Paris polyphylla var. yunnanensis on the ITS2, Polygonatum kingianum and Bletilla striata on the psbA-trnH, using Primer 5.0. And these specific identification primers could amplify special bands respectively at 130bp, 331bp and 508bp, while adulterants could not amplify the bands. The results showed that this method could respectively distinguish three herbal medicines and their adulterants. And after many experiments, the method had good repeatability. The allele-specific PCR identification method established in this study is easy to operate and fast in identification. This method can distinguish three kinds of traditional Chinese medicines and their adulterants, which provides a reference for the identification of other traditional Chinese medicines, so as to further protect the Chinese herbal medicine industry from economically motivated adulteration.},
year = {2022}
}
TY - JOUR T1 - Identification of Three Chinese Herbal Medicines and Their Adulterants Based on Allele-Specific Diagnostic PCR AU - Hao Chen AU - Xiao-Lei Chen AU - Jian-Yun Su AU - Yan-Ying Li AU - Xiao-Bin Hu AU - Zhe Xu AU - Hua-Li Qian AU - Lei Zhang AU - Jia-Hong Dong AU - Peng-Zhang Ji Y1 - 2022/11/29 PY - 2022 N1 - https://doi.org/10.11648/j.jps.20221006.13 DO - 10.11648/j.jps.20221006.13 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 214 EP - 221 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20221006.13 AB - Owing to the high pharmacological and economic value, the Chinese medicinal herbs: Paris polyphylla var. yunnanensis, Polygonatum kingianum and Bletilla striata, whose quality has been damaged by their adulterants. In this study, the allele-specific diagnostic PCR method was used to identify three herbal medicines and their adulterants. And the nuclear gene ITS2 sequence and chloroplast gene psbA-trnH sequence, as international common barcode sequences, are widely used in the molecular identification of medicinal plants. Therefore, three pair of specific PCR primers were respectively designed according to the sequence of Paris polyphylla var. yunnanensis on the ITS2, Polygonatum kingianum and Bletilla striata on the psbA-trnH, using Primer 5.0. And these specific identification primers could amplify special bands respectively at 130bp, 331bp and 508bp, while adulterants could not amplify the bands. The results showed that this method could respectively distinguish three herbal medicines and their adulterants. And after many experiments, the method had good repeatability. The allele-specific PCR identification method established in this study is easy to operate and fast in identification. This method can distinguish three kinds of traditional Chinese medicines and their adulterants, which provides a reference for the identification of other traditional Chinese medicines, so as to further protect the Chinese herbal medicine industry from economically motivated adulteration. VL - 10 IS - 6 ER -
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