Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars

Galal Bakr Anis; Zeinab Abdelnaby Kalboush; Ahmed Ibrahem Elsherif; Raghda Mohamed Sakran

doi:doi:10.11648/j.jps.20221004.14

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Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars

Galal Bakr Anis, Zeinab Abdelnaby Kalboush, Ahmed Ibrahem Elsherif, Raghda Mohamed Sakran

Published in Journal of Plant Sciences (Volume 10, Issue 4)

Received: 8 April 2022 Accepted: 2 August 2022 Published: 31 August 2022

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Abstract

Sakha 101 is one such short grained common Egyptian rice cultivar and known for its exquisite quality, however, is highly susceptible to blast disease that has led to considerable decline in its area. Sakha 101 was crossed to a blast gene donor line, HR5824-B-3-2-3 and followed through backcross-breeding method that helped to incorporate blast resistance genes and finally Sakha 108 was released as improved version of the most widespread Egyptian commercial rice Sakha 101. The study was to evaluate Sakha 101 and Sakha 108 for high yielding, blast resistance, and effective resistance genes to Pyricularia oryzae as well as assessment of genetic divergence based on genomic in these cultivars. There is a slight increase in the values of Sakha 108 than Sakha 101 in the most of studied traits. Also under this study, seventy isolates were identified as eight main groups i.e., IA, IB, IC, ID, IF, IG, IH and II, but ID group was considered the most common races. On the other hand, Pi-Z and Pii - Pi-k^s resistance genes were the most effective genes to blast fungus. Sakha 108 proved resistance for all tested isolates under greenhouse condition compared with Sakha 101 which exhibited susceptible to 70% of tested isolates. On genomic level, out of 242 markers across the 7 chromosomes; only 6 markers (RM8236, RM13611, RM3839, RM17377, RM160 and RM27154) produced clear fragments and polymorphism between cultivars (Sakha 101 and Sakha 108) and were used to construct a genetic linkage map. A total of 39 candidate genes were identified around their regions on each chromosome. These results enrich our understanding of the differences between Sakha 101 and Sakha 108 and also provide a foundation for selecting candidate for marker-assisted selection breeding in rice.

Published in	Journal of Plant Sciences (Volume 10, Issue 4)
DOI	10.11648/j.jps.20221004.14
Page(s)	150-164
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Rice, Yield, Blast Disease, Resistance Genes, Genomic Regions

References

[1]	Adair, C. R. (1952). The McGill miller method for determining the milled quality of small samples of rice. Rice Jornal, 55 (2): 21-23.
[2]	Ahn, S. N., Kim, Y. K., Hong, H. C., Han, S. S., Kwon, S. J., Choi, H. C., Moon, H. P., and McCouch, S. R. 2000. Molecular mapping of a new gene for resistance to rice blast (Pyricularia grisea Sacc.). Euphytica 116: 17-22.
[3]	Anandan, A., Anumalla M., Pradhan S. K. and Ali J. (2016). Population Structure, Diversity and Trait Association Analysis in Rice (Oryza sativa L.) Germplasm for Early Seedling Vigor (ESV) Using Trait Linked SSR Markers. PLoS ONE 11 (3): e0152406. doi: 10.1371/journal.pone.0152406.
[4]	Anis, G. B., Amr A. Hassan and Ahmed S. Taha (2021). Genetic Diversity Analysis and Molecular Characterization of Elite Rice Promising Lines for Yield, Blast Disease Resistance and Rice Stem Borer. J. of Plant Protection and Pathology, Mansoura Univ., Vol 12 (10): 733 -743, 2021.
[5]	Anis, G., Zhang, Y. X., Xu, X. M., Fiaz, S., Wu, W. X., Rahman, M. H., Riaz, A., Chen, D., Shen, X. H., Zhan, X. D., Cao, L. Y. and Cheng, S. H. (2018a). QTL analysis for rice seedlings under Nitrogen deficiency using Chromosomal Segment Substitution Lines. Pakistan Journal of Botany, 50 (2): 537-544.
[6]	Anis, G.; Zhang, Y.; Wang, H.; Li, Z.; Wu, W.; Sun, L.; Riaz, A.; Cao, L.; Cheng, S. (2018b). Genomic Regions Analysis of Seedling Root Traits and Their Regulation in Responses to Phosphorus Deficiency Tolerance in CSSL Population of Elite Super Hybrid Rice. International Journal of Molecular Sciences, 19 (5): 1-14.
[7]	Atkins, J. G., Robert, A. L., Adair, C. R., Goto, K., Kozako, T., Yanagida, R., Yamada, Y. and Matsumoto, S. (1967). An international set of rice varieties for differentiating races of Pyricularia oryzae. Phytopathology, 57: 298-301.
[8]	Awadallah, Marvet, M., Taha A. S. and Tahoon A. M. (2021). Genetic variability, insect and disease resistance of some promising rice genotypes. J. of Plant Protection and Pathology, Mansoura Univ., 12 (6): 413-421.
[9]	Chen, D. H., Vina, M., Inukai, T., Mackil, D. J., Ronald, P. C., and Nelson, R. J. 1999. Molecular mapping of the blast resistance gene, Pi44 (t), in a line derived from a durably resistant rice cultivar. Theor. Appl. Genet. 98: 1046-1053.
[10]	Cheng, X. X., Cheng J. P., Huang X., Lai Y. Y., Wang L., Du W. L., et al. (2013). Dynamic quantitative trait loci analysis of seed reserve utilization during three germination stages in rice. PLoS One, 8 (11): e80002. doi: 10.1371/journal.pone.0080002 PMID: 24244592.
[11]	Creste, S., Tulmann Neto A., Figueira A. (2001). Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining. Plant Mol. Biol. Rep., 19 (4): 299–306.
[12]	Fuentes, J. L., Correa-Victoria, J. F., Escobar, F., Prado, G., Aricapa, G., Duque, M. C., and Tohme, J. 2008. Identification of microsatellite markers linked to the blast resistance gene Pi-1 (t) in rice. Euphytica 160: 295-304.
[13]	Fukuoka, S., N. Saka, H. Koga, K. Ono, T. Shimizu, K. Ebana, N. Hayashi, A. Takahashi, H. Hirochika, K. Okuno and M. Yano (2009). Loss of Function of a Proline-Containing Protein Confers Durable Disease Resistance in Rice. Science, New Series, 325 (5943): 998-1001.
[14]	Hammoud S. A; M. I. Aboyessef; S. E. Sedeek; R. A. EL-Namaky; M. M. El-Malkey; A. B. EL-Abd; M. H. Ammar et al. (2020). Sakha108 Egyptian rice variety Japonica type high yielding and resistant to blast. J. of Plant Production, Mansoura Univ., 11 (11): 1153-1162.
[15]	Hammoud, S. A. A.; M. M. El-Malky; H. M. Hassan and S. E. M. Sedeek (2013). Assessment and evaluation of genetic diversity of some lines developed from Sakha 101 cv. of rice by using SSR markers. J. Agric. Chem. and Biotechn., Mansoura Univ., 4 (12): 371-387.
[16]	Hussain, S., M. Ramzan, M. Aslam, Z. Manzoor and M. Ehsan Safdar (2005). Effect of Various Stand Establishment Method on Yield and Yield Components of Rice. Proceedings of the International Seminar on Rice Crop. October 2-3. Rice Research Institute, Kala Shah Kau, Pakistan. pp. 212-220.
[17]	IRRI, (2013). Standard evaluation system for rice (SES). Manila: International Rice Research Institute.
[18]	Islam, A., Y. Zhang, G. Anis, M. H. Rani, W. Anley, X. Shen, L. Cao, S. Cheng and W. Wu (2020). Mapping and validation of a major quantitative trait locus qRN5a associated with increasing root number under low potassium in rice. Plant Growth Regulation, https: //doi.org/10.1007/s10725-020-00574-8
[19]	Jamal, I. H. K., A. Bari, S. Khan and Z. Islam (2009). Genetic variation for yield and yield components in rice. ARPN Journal of Agricultural and Biological Science, 4 (6): 60-64.
[20]	Kalboush, A. Zeinab (2019). Resistance of rice genotypes to the blast fungus and the associated biochemical changes. Egypt. J. Agric. Res., 97 (1): 39-55.
[21]	Khan, G. H., Asif Bashir Shikari1, Rakesh Vaishnavi, Sofi Najeeb, Bilal A. Padder, Zahoor A. Bhat4, Ghulam A. Parray3, Mohammad Ashraf Bhat5, Ram Kumar6 & Nagendra K. Singh (2018). Marker-assisted introgression of three dominant blast resistance genes into an aromatic rice cultivar Mushk Budji. SCIeNTIFIC REPOrTS 8 (4091): 1-13. DOI: 10.1038/s41598-018-22246-4.
[22]	Koch, E. and Slusarenko, A. (1990). Arabidopsis is susceptible to infection by a downy mildew fungus. Plant Cell 2: 437–445.
[23]	Kulkarni, S. R., Balachandran S. M., Ulaganathan, K. et al. (2020). Molecular mapping of QTLs for yield related traits in recombinant inbred line (RIL) population derived from the popular rice hybrid KRH-2 and their validation through SNP genotyping. Sci Rep 10, 13695 (2020). https://doi.org/10.1038/s41598-020-70637-3
[24]	Kwon, J. O. and S. G. Lee (2002). Real-time micro-weather factors of growing field to the epidemics of rice blast. Res. Plant. Dis., 8: 199–206 (in Korean, English abstract).
[25]	Li, L. Y., Wang, L., Jing, J. X., Li, Z. Q., Lin, F., Huang, L. F., and Pan, Q. H. 2007. The Pikm gene, conferring stable resistance to isolates of Magnaporthe oryzae, was finely mapped in a crossover-cold region on rice chromosome 11. Mol. Breed. 20: 179-188.
[26]	Li, P. F., Shi, X. L., Wang, J. F., Liu, C., and Zhang, H. S. 2007. Molecular mapping of rice blast resistance gene in a japonica landrace Heikezijing from the Taihu Lake area, China. Chin. J. Rice Sci. 21: 579-584.
[27]	Li, Y. B.; C. J. Wu; G. H. Jiang; L. Q. Wang and Y. Q. He (2007). Dynamic analyses of rice blast resistance for the assessment of genetic and environmental effects. Plant Breeding, 126: 541–547.
[28]	Luo, Z. Y., Zhou G., Chen X. H., Lu Q. H., Hu W. X. (2001). Isolation of high-quality genomic DNA from plants. Bull Hunan Med Univ., 26: 178-180.
[29]	Mackill, D., and Bonman, J. 1992. Inheritance of blast resistance in nearisogenic lines of rice. Phytopathology 82: 746-749.
[30]	McCouch, S. R., Nelson, R. J., Thome, J., and Zeigler, R. S. 1994. Mapping of blast-resistance genes in rice. Pages 167-187 in: Rice Blast Disease. R. S. Zeigler, S. A. Leong, and P. S. Teng, eds. CAB International, Wallingford, UK.
[31]	Nazmy, N. B.; A. Ammar and A. Ezzat (2014). Study of some cooking and eating quality characters on some Egyptian rice genotype. AGRáRTUDoMánYI KözLEMénYEK, (59): 77-82.
[32]	Orjuela, J., T. Deless, O. Kolade, S. Chéron, A. Ghesquière and L. Albar (2013). A Recessive Resistance to Rice yellow mottle virus Is Associated with a Rice Homolog of the CPR5 Gene, a Regulator of Active Defense Mechanisms. Molecular Plant-Microbe Interactions, 26 (12): 1455–1463.
[33]	Ou, S. H. (1985). Blast. In: Ou SH, editor. Rice diseases. Surrey, Kew: Commonwealth Mycological Institute; 1985. p. 272-286.
[34]	Pao, X., Zhang Y. X., Lou X. Y., Zhu A. K., Chen Y. Y., Sun B., Yu P., Cheng S. H., Cao L. Y., Zhan X. D. (2019). Mapping and genetic validation of a grain size QTL qGS7.1 in rice (Oryza sativa L.). Journal of Integrative Agriculture, 18 (8): 1838–1850. https://doi.org/10.1016/S2095-3119(18)62113-6
[35]	Rice Chromosomes 11 and 12 Sequencing Consortia. 2005. The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications. BMC Biol. 3: 20-37.
[36]	Royal Society, (2009). Reaping the benefits: science and the sustainable intensification of global agriculture. Report, October 2009.
[37]	RRTC, (2016). Rice Research and Training Center. Technical bulletin of rice research and training center, Agricultural Research Center, Ministry of agricultural and land Reclamation, Egypt.
[38]	RRTC, (2020). Rice Research and Training Center. Technical bulletin of rice research and training center, Agricultural Research Center, Ministry of agricultural and land Reclamation, Egypt.
[39]	Sallaud, C., Lorieux, M., Roumen, E., Tharreau, D., Berruyer, R., Svestasrani, P., Garsmeur, O., Ghesquiere, A., and Notteghem, J. L. 2003. Identification of five new blast resistance genes in the highly blast resistant rice variety IR64 using a QTL mapping strategy. Theor. Appl. Genet. 106: 794-803.
[40]	Sedeek, S. E. M. and S. M. El-Wahsh. 2015. Performance of some agronomic traits of selected rice breeding lines and its reaction to blast disease. Agric. Res. Kafrelsheikh.univ., 41 (1): 167-180.
[41]	Sehly, M. R., El-Wahsh S. M., Badr E. A. S., EL-Malky M. M. H, EL-Shafey R. A. S. and Aidy I. R. (2008). Evaluation of certain Egyptian rice cultivars to blast disease incidence during fourteen years in Egypt, J. Agric. Sci., Mansoura Univ., 33 (4): 2533-2547.
[42]	Sehly, M. R.; Z. H. Osman and E. A. Salem (2002). Rice diseases. In: rice in Egypt (ed. Theresa A. Castillo). Rice Research and Training Center, Sakha, Kafr El-Sheikh, Egypt. pp. 198-247.
[43]	Shabana, Y. M., El-Wahsh S. M., Abdelkhalik A. F., Fayzalla1 S. A. and Hassan A. A. (2013). Physiological races of rice blast and host resistant genes under Egyptian condition, J. Plant Prot. and Path., Mansoura Univ., 4 (8): 709-720.
[44]	Shar, T., Z. Sheng, U. Ali, S. Fiaz, X. Wei, L. Xie, G. Jiao, F. Ali, G. Shao, S. Hu, P. Hu and S. Tang (2020). Mapping quantitative trait loci associated with starch paste viscosity attributes by using double haploid populations of rice (Oryza sativa L.). Journal of Integrative Agriculture, 19 (7): 1691-1703.
[45]	Sharma, T. R., Madhav, M. S., Singh, B. K., Shanker, P., Jana, T. K., Dalal, V., Pandit, A., Singh, A., Gaikwad, K., Upreti, H. C., and Singh, N. K. 2005. High-resolution mapping, cloning and molecular characterization of the Pi-kh gene of rice, which confers resistance to Magnaporthe grisea. Mol. Genet. Genomics 274: 569-578.
[46]	Shi, X., Wang, J., Bao, Y., Li, P., Xie, L., Huang, J., and Zhang, H. (2010). Identification of the quantitative trait loci in japonica rice landrace Heikezijing responsible for broad-spectrum resistance to rice blast. Phytopathology 100: 822-829.
[47]	Skamnioti, P. and S. J. Gurr (2009). Against the grain safe guarding rice from rice blast disease. Department of plant sciences, University of Oxford, Oxi 3RB, UK.
[48]	Sun, Z., Liu, Y., Xiao, S. et al. (2017). Identification of quantitative trait loci for resistance to rice black-streaked dwarf virus disease and small brown planthopper in rice. Mol. Breeding, 37: 72. https://doi.org/10.1007/s11032-017-0669-x
[49]	Tabien, R. E., Li, Z., Paterson, A. H., Marchetti, M. A., Stansel, J. W., and Pinson, S. R. M. 2000. Mapping of four major rice blast resistance genes from ‘Lemont’ and ‘Teqing’ and evaluation of their combinatorial effect for field resistance. Theor. Appl. Genet. 101: 1215-1225.
[50]	Tahmina, S., SHENG Zhong-hua, Umed Ali, Sajid Fiaz, WEI Xiang-jin, XIE Li-hong, JIAO Gui-ai, Fahad Ali, SHAO Gao-neng, HU Shi-kai, HU Pei-song, TANG Shao-qing (2020). Mapping quantitative trait loci associated with starch paste viscosity attributes by using double haploid populations of rice (Oryza sativa L.). Journal of Integrative Agriculture, 19 (7): 1691-1703.
[51]	Wang, J. C., Y. Jiab, J. W. Wen, W. P. Liu, X. M. Liu, L. Li, Z. Y. Jiang, J. H. Zhang, X. L. Guo and Ren, J. P. (2013). Identification of rice blast resistance genes using international monogenic differentials. Crop Protection, 45: 109-116.
[52]	Zahid, A. M., M. Akhtar, M. Sabar, M. Anwar and M. Ahmad (2005). Interrelation-ship among Yield and Economic Traits in Fine Grain Rice. Proceedings of the International Seminar on Rice Crop. October 2-3. Rice Research Institute, Kala Shah Kau, Pakistan. pp. 21-24.
[53]	Zenbayashi-Sawata, K., Ashizawa, T., and Koizumi, S. 2005. Pi34- AVRPi34: a new gene-for-gene interaction for partial resistance in rice to blast caused by Magnaporthe grisea. J. Gen. Plant Pathol. 71: 395-401.
[54]	Zenbayashi-Sawata, K., Fukuoka, S., Katagiri, S., Fujisawa, M., Matsumoto, T., Ashizawa, T., and Koizumi, S. 2007. Genetic and physical mapping of the partial resistance gene Pi34, to blast in rice. Phytopathology 97: 598-602.
[55]	Zhang, B., W. Ye, D. Ren, P. Tian, Y. Peng, Y. Gao, B. Ruan, L. Wang, G. Zhang, L. Guo, Q. Qian and Z. Gao (2015). Genetic analysis of flag leaf size and candidate genes determination of a major QTL for flag leaf width in rice. Rice, 8: 2.
[56]	Zhang, J. F., Ling, Z. Z., Wang, G. Y., and Xie, H. A. 2006. Mapping blast-resistance genes of japonica rice Yunyin by SSR markers. Mol. Plant Breed. 4: 359-364.
[57]	Zhang, Y., G. Anis, R. Wang, W. Wu, N. Yu, X. Shen, X. Zhan, S. Cheng and L. Cao (2017). Genetic dissection of QTL against Phosphate deficiency in the hybrid rice 'Xieyou9308'. Plant Growth Regulation, 84 (1): 123–133.
[58]	Zhou, J., A. You, Z. Ma, L. Zhu and G. He (2012). Association analysis of important agronomic traits in japonica rice germplasm. African Journal of Biotechnology, 11 (12): 2957-2970.
[59]	Zhou, T., Wang, Y., Chen, J. Q., Araki, H., Jing, Z., Jiang, K., Shen, J., and Tian, D. 2004. Genome-wide identification of NBS genes in japonica rice reveals significant expansion of divergent non-TIR NBS-LRR genes. Mol. Genet. Genomics 271: 402-415.

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Galal Bakr Anis, Zeinab Abdelnaby Kalboush, Ahmed Ibrahem Elsherif, Raghda Mohamed Sakran. (2022). Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars. Journal of Plant Sciences, 10(4), 150-164. https://doi.org/10.11648/j.jps.20221004.14

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Galal Bakr Anis; Zeinab Abdelnaby Kalboush; Ahmed Ibrahem Elsherif; Raghda Mohamed Sakran. Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars. J. Plant Sci. 2022, 10(4), 150-164. doi: 10.11648/j.jps.20221004.14

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Galal Bakr Anis, Zeinab Abdelnaby Kalboush, Ahmed Ibrahem Elsherif, Raghda Mohamed Sakran. Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars. J Plant Sci. 2022;10(4):150-164. doi: 10.11648/j.jps.20221004.14

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@article{10.11648/j.jps.20221004.14,
  author = {Galal Bakr Anis and Zeinab Abdelnaby Kalboush and Ahmed Ibrahem Elsherif and Raghda Mohamed Sakran},
  title = {Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars},
  journal = {Journal of Plant Sciences},
  volume = {10},
  number = {4},
  pages = {150-164},
  doi = {10.11648/j.jps.20221004.14},
  url = {https://doi.org/10.11648/j.jps.20221004.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20221004.14},
  abstract = {Sakha 101 is one such short grained common Egyptian rice cultivar and known for its exquisite quality, however, is highly susceptible to blast disease that has led to considerable decline in its area. Sakha 101 was crossed to a blast gene donor line, HR5824-B-3-2-3 and followed through backcross-breeding method that helped to incorporate blast resistance genes and finally Sakha 108 was released as improved version of the most widespread Egyptian commercial rice Sakha 101. The study was to evaluate Sakha 101 and Sakha 108 for high yielding, blast resistance, and effective resistance genes to Pyricularia oryzae as well as assessment of genetic divergence based on genomic in these cultivars. There is a slight increase in the values of Sakha 108 than Sakha 101 in the most of studied traits. Also under this study, seventy isolates were identified as eight main groups i.e., IA, IB, IC, ID, IF, IG, IH and II, but ID group was considered the most common races. On the other hand, Pi-Z and Pii - Pi-ks resistance genes were the most effective genes to blast fungus. Sakha 108 proved resistance for all tested isolates under greenhouse condition compared with Sakha 101 which exhibited susceptible to 70% of tested isolates. On genomic level, out of 242 markers across the 7 chromosomes; only 6 markers (RM8236, RM13611, RM3839, RM17377, RM160 and RM27154) produced clear fragments and polymorphism between cultivars (Sakha 101 and Sakha 108) and were used to construct a genetic linkage map. A total of 39 candidate genes were identified around their regions on each chromosome. These results enrich our understanding of the differences between Sakha 101 and Sakha 108 and also provide a foundation for selecting candidate for marker-assisted selection breeding in rice.},
 year = {2022}
}

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TY  - JOUR
T1  - Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars
AU  - Galal Bakr Anis
AU  - Zeinab Abdelnaby Kalboush
AU  - Ahmed Ibrahem Elsherif
AU  - Raghda Mohamed Sakran
Y1  - 2022/08/31
PY  - 2022
N1  - https://doi.org/10.11648/j.jps.20221004.14
DO  - 10.11648/j.jps.20221004.14
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 150
EP  - 164
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20221004.14
AB  - Sakha 101 is one such short grained common Egyptian rice cultivar and known for its exquisite quality, however, is highly susceptible to blast disease that has led to considerable decline in its area. Sakha 101 was crossed to a blast gene donor line, HR5824-B-3-2-3 and followed through backcross-breeding method that helped to incorporate blast resistance genes and finally Sakha 108 was released as improved version of the most widespread Egyptian commercial rice Sakha 101. The study was to evaluate Sakha 101 and Sakha 108 for high yielding, blast resistance, and effective resistance genes to Pyricularia oryzae as well as assessment of genetic divergence based on genomic in these cultivars. There is a slight increase in the values of Sakha 108 than Sakha 101 in the most of studied traits. Also under this study, seventy isolates were identified as eight main groups i.e., IA, IB, IC, ID, IF, IG, IH and II, but ID group was considered the most common races. On the other hand, Pi-Z and Pii - Pi-ks resistance genes were the most effective genes to blast fungus. Sakha 108 proved resistance for all tested isolates under greenhouse condition compared with Sakha 101 which exhibited susceptible to 70% of tested isolates. On genomic level, out of 242 markers across the 7 chromosomes; only 6 markers (RM8236, RM13611, RM3839, RM17377, RM160 and RM27154) produced clear fragments and polymorphism between cultivars (Sakha 101 and Sakha 108) and were used to construct a genetic linkage map. A total of 39 candidate genes were identified around their regions on each chromosome. These results enrich our understanding of the differences between Sakha 101 and Sakha 108 and also provide a foundation for selecting candidate for marker-assisted selection breeding in rice.
VL  - 10
IS  - 4
ER  -

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Author Information

Galal Bakr Anis

Rice Research Department, Field Crops Research Institute, Agricultural Research Center, Kafr El Sheikh, Egypt
Zeinab Abdelnaby Kalboush

Rice Pathology Department, Plant Pathology Research Institute, Agricultural Research Center, Kafr El Sheikh, Egypt
Ahmed Ibrahem Elsherif

Rice Research Department, Field Crops Research Institute, Agricultural Research Center, Kafr El Sheikh, Egypt
Raghda Mohamed Sakran

Rice Research Department, Field Crops Research Institute, Agricultural Research Center, Kafr El Sheikh, Egypt

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Galal Bakr Anis, Zeinab Abdelnaby Kalboush, Ahmed Ibrahem Elsherif, Raghda Mohamed Sakran. (2022). Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars. Journal of Plant Sciences, 10(4), 150-164. https://doi.org/10.11648/j.jps.20221004.14

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Galal Bakr Anis; Zeinab Abdelnaby Kalboush; Ahmed Ibrahem Elsherif; Raghda Mohamed Sakran. Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars. J. Plant Sci. 2022, 10(4), 150-164. doi: 10.11648/j.jps.20221004.14

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AMA Style

Galal Bakr Anis, Zeinab Abdelnaby Kalboush, Ahmed Ibrahem Elsherif, Raghda Mohamed Sakran. Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars. J Plant Sci. 2022;10(4):150-164. doi: 10.11648/j.jps.20221004.14

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@article{10.11648/j.jps.20221004.14,
  author = {Galal Bakr Anis and Zeinab Abdelnaby Kalboush and Ahmed Ibrahem Elsherif and Raghda Mohamed Sakran},
  title = {Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars},
  journal = {Journal of Plant Sciences},
  volume = {10},
  number = {4},
  pages = {150-164},
  doi = {10.11648/j.jps.20221004.14},
  url = {https://doi.org/10.11648/j.jps.20221004.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20221004.14},
  abstract = {Sakha 101 is one such short grained common Egyptian rice cultivar and known for its exquisite quality, however, is highly susceptible to blast disease that has led to considerable decline in its area. Sakha 101 was crossed to a blast gene donor line, HR5824-B-3-2-3 and followed through backcross-breeding method that helped to incorporate blast resistance genes and finally Sakha 108 was released as improved version of the most widespread Egyptian commercial rice Sakha 101. The study was to evaluate Sakha 101 and Sakha 108 for high yielding, blast resistance, and effective resistance genes to Pyricularia oryzae as well as assessment of genetic divergence based on genomic in these cultivars. There is a slight increase in the values of Sakha 108 than Sakha 101 in the most of studied traits. Also under this study, seventy isolates were identified as eight main groups i.e., IA, IB, IC, ID, IF, IG, IH and II, but ID group was considered the most common races. On the other hand, Pi-Z and Pii - Pi-ks resistance genes were the most effective genes to blast fungus. Sakha 108 proved resistance for all tested isolates under greenhouse condition compared with Sakha 101 which exhibited susceptible to 70% of tested isolates. On genomic level, out of 242 markers across the 7 chromosomes; only 6 markers (RM8236, RM13611, RM3839, RM17377, RM160 and RM27154) produced clear fragments and polymorphism between cultivars (Sakha 101 and Sakha 108) and were used to construct a genetic linkage map. A total of 39 candidate genes were identified around their regions on each chromosome. These results enrich our understanding of the differences between Sakha 101 and Sakha 108 and also provide a foundation for selecting candidate for marker-assisted selection breeding in rice.},
 year = {2022}
}

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TY  - JOUR
T1  - Genomic Characterization and Identification of Effective Blast Resistant Genes for Sakha 101 and Sakha 108 as High Yielding Egyptian Rice Cultivars
AU  - Galal Bakr Anis
AU  - Zeinab Abdelnaby Kalboush
AU  - Ahmed Ibrahem Elsherif
AU  - Raghda Mohamed Sakran
Y1  - 2022/08/31
PY  - 2022
N1  - https://doi.org/10.11648/j.jps.20221004.14
DO  - 10.11648/j.jps.20221004.14
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 150
EP  - 164
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20221004.14
AB  - Sakha 101 is one such short grained common Egyptian rice cultivar and known for its exquisite quality, however, is highly susceptible to blast disease that has led to considerable decline in its area. Sakha 101 was crossed to a blast gene donor line, HR5824-B-3-2-3 and followed through backcross-breeding method that helped to incorporate blast resistance genes and finally Sakha 108 was released as improved version of the most widespread Egyptian commercial rice Sakha 101. The study was to evaluate Sakha 101 and Sakha 108 for high yielding, blast resistance, and effective resistance genes to Pyricularia oryzae as well as assessment of genetic divergence based on genomic in these cultivars. There is a slight increase in the values of Sakha 108 than Sakha 101 in the most of studied traits. Also under this study, seventy isolates were identified as eight main groups i.e., IA, IB, IC, ID, IF, IG, IH and II, but ID group was considered the most common races. On the other hand, Pi-Z and Pii - Pi-ks resistance genes were the most effective genes to blast fungus. Sakha 108 proved resistance for all tested isolates under greenhouse condition compared with Sakha 101 which exhibited susceptible to 70% of tested isolates. On genomic level, out of 242 markers across the 7 chromosomes; only 6 markers (RM8236, RM13611, RM3839, RM17377, RM160 and RM27154) produced clear fragments and polymorphism between cultivars (Sakha 101 and Sakha 108) and were used to construct a genetic linkage map. A total of 39 candidate genes were identified around their regions on each chromosome. These results enrich our understanding of the differences between Sakha 101 and Sakha 108 and also provide a foundation for selecting candidate for marker-assisted selection breeding in rice.
VL  - 10
IS  - 4
ER  -

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