Standardization, Optimization of Formulation and In-vitro Evaluation of Anti-urolithiasis Activity of <i>Citrus medica </i>Extract

Choudhary Dimple; Naik Archana

doi:doi:10.11648/j.jddmc.20220803.11

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Standardization, Optimization of Formulation and In-vitro Evaluation of Anti-urolithiasis Activity of Citrus medica Extract

Choudhary Dimple, Naik Archana

Published in Journal of Drug Design and Medicinal Chemistry (Volume 8, Issue 3)

Received: 29 May 2022 Accepted: 22 June 2022 Published: 28 July 2022

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Abstract

Citrus medica is very popular in traditional system of medicine specially in China and India. The fruit possesses various medicinal activities. Many studies have been conducted on Citrus medica to authenticate its use as multipurpose medicinal agent. Aim of current study was to standardize the fruit of Citrus medica followed by evaluation of anti-urolithiasis activity and preparation of formulation. Flavonoid rich extract of Citrus medicawas prepared by maceration followed by liquid liquid extraction. Qualitative analysis was carried by TLC. Extract was then subjected to spray drying process to get free following powder for formulation. Standardization of flavonoid rich extract of fruit of CM was carried out by HPTLC method using toluene: ethyl acetate: formic acid (6:4:0.1 v/v) asmobile phaseand Silica gel GF 254nm as stationary phase with biomarker i.e. naringenin. Detection was carried out at 281 nm. Total flavonoid content was estimated by UV spectroscopy by considering quercetin as standard. Anti-urolithiasis activity was evaluated by in-vitro model using cystone as standard agent used for kidney stone. Extract showed potent activity as compared to standard. Spray dried powder of flavonoid rich extract was then incorporated in tablet formulation. Evaluation of tablet was carried out as per guidelines and it passes as per the specifications. Hence Citrus medica fruits which are traditionally popular is proved as potent agent as well as standardization give assurity about quality of the herbal formulation and can be used as potent anti-urolithiatic agent in ayurvedic era.

Published in	Journal of Drug Design and Medicinal Chemistry (Volume 8, Issue 3)
DOI	10.11648/j.jddmc.20220803.11
Page(s)	34-40
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Urolithiasis, Citrus medica, Citron, Bajura, Bijapura, Kidney Stone, Standardization

References

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[4]	Venturini N, Barboni T, Curk F, Costa J, Paolini J. Volatile and flavonoid composition of the peel of Citrus medica L. var. Corsican fruit for quality assessment of its liqueur. Food technology and biotechnology. 2014 Dec 15; 52 (4): 403-10.
[5]	Panara K, Joshi K, Nishteswar K. A review on phytochemical and pharmacological properties of Citrus medica Linn. Int. J. Pharm. Biol. Arch. 2012; 3 (6): 1292-7.
[6]	Manisha V Kalariya.; Rakesh P Prajapati.; “Pharmacognostic and phytochemical evaluation of Bijapur (Citrus medica Linn.) fruit” Journal of Pharmacognosy and Phytochemistry 2019; 8 (3): 4159-4164.
[7]	Royal Horticultural Society (Great Britain) (1894). "Scientific Committee, March 28, 1893: The Antiquity of the Citron in Egypt". Journal of the Royal Horticultural Society. 16.
[8]	Hetta MH, El-Alfy TS, Yassin NZ, Abdel-Rahman RF, Kadry EM. Phytochemical and antihyperglycemic studies on Citrus medica L. leaves (Etrog) growing in Egypt. Int J PharmacognPhytochem Res. 2013; 5: 271-7.
[9]	Nagaraju B, Anand SC, Ahmed N, Chandra JN, Ahmed F, Padmavathi GV. Antiulcer activity of aqueous extract of Citrus medica Linn. fruit against ethanol induced ulcer in rats. Advances in Biological Research. 2012; 6 (1): 24-9.
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[14]	Peikes A. The Formation and Manifestation of Kidney Stones. The Science Journal of the Lander College of Arts and Sciences. 2020; 14 (1): 79-85.
[15]	https://www.mayoclinic.org/diseases-conditions/kidney-stones/symptoms-causes/syc-20355755
[16]	Chavada KS, Fadadu KN, Patel KV, Patel KG, Gandhi TR. EFFECT OF FLAVANOID RICH FRACTION OF CITRUS MEDICA LINN. (RUTACEA) ON ETHYLENE GLYCOL INDUCED UROLITHIASIS IN RATS. Journal of Drug Delivery and Therapeutics. 2012 Jul 15; 2 (4).
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APA Style

Choudhary Dimple, Naik Archana. (2022). Standardization, Optimization of Formulation and In-vitro Evaluation of Anti-urolithiasis Activity of Citrus medica Extract. Journal of Drug Design and Medicinal Chemistry, 8(3), 34-40. https://doi.org/10.11648/j.jddmc.20220803.11

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ACS Style

Choudhary Dimple; Naik Archana. Standardization, Optimization of Formulation and In-vitro Evaluation of Anti-urolithiasis Activity of Citrus medica Extract. J. Drug Des. Med. Chem. 2022, 8(3), 34-40. doi: 10.11648/j.jddmc.20220803.11

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AMA Style

Choudhary Dimple, Naik Archana. Standardization, Optimization of Formulation and In-vitro Evaluation of Anti-urolithiasis Activity of Citrus medica Extract. J Drug Des Med Chem. 2022;8(3):34-40. doi: 10.11648/j.jddmc.20220803.11

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@article{10.11648/j.jddmc.20220803.11,
  author = {Choudhary Dimple and Naik Archana},
  title = {Standardization, Optimization of Formulation and In-vitro Evaluation of Anti-urolithiasis Activity of Citrus medica Extract},
  journal = {Journal of Drug Design and Medicinal Chemistry},
  volume = {8},
  number = {3},
  pages = {34-40},
  doi = {10.11648/j.jddmc.20220803.11},
  url = {https://doi.org/10.11648/j.jddmc.20220803.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jddmc.20220803.11},
  abstract = {Citrus medica is very popular in traditional system of medicine specially in China and India. The fruit possesses various medicinal activities. Many studies have been conducted on Citrus medica to authenticate its use as multipurpose medicinal agent. Aim of current study was to standardize the fruit of Citrus medica followed by evaluation of anti-urolithiasis activity and preparation of formulation. Flavonoid rich extract of Citrus medicawas prepared by maceration followed by liquid liquid extraction. Qualitative analysis was carried by TLC. Extract was then subjected to spray drying process to get free following powder for formulation. Standardization of flavonoid rich extract of fruit of CM was carried out by HPTLC method using toluene: ethyl acetate: formic acid (6:4:0.1 v/v) asmobile phaseand Silica gel GF 254nm as stationary phase with biomarker i.e. naringenin. Detection was carried out at 281 nm. Total flavonoid content was estimated by UV spectroscopy by considering quercetin as standard. Anti-urolithiasis activity was evaluated by in-vitro model using cystone as standard agent used for kidney stone. Extract showed potent activity as compared to standard. Spray dried powder of flavonoid rich extract was then incorporated in tablet formulation. Evaluation of tablet was carried out as per guidelines and it passes as per the specifications. Hence Citrus medica fruits which are traditionally popular is proved as potent agent as well as standardization give assurity about quality of the herbal formulation and can be used as potent anti-urolithiatic agent in ayurvedic era.},
 year = {2022}
}

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TY - JOUR
T1 - Standardization, Optimization of Formulation and In-vitro Evaluation of Anti-urolithiasis Activity of Citrus medica Extract
AU - Choudhary Dimple
AU - Naik Archana
Y1 - 2022/07/28
PY - 2022
N1 - https://doi.org/10.11648/j.jddmc.20220803.11
DO - 10.11648/j.jddmc.20220803.11
T2 - Journal of Drug Design and Medicinal Chemistry
JF - Journal of Drug Design and Medicinal Chemistry
JO - Journal of Drug Design and Medicinal Chemistry
SP - 34
EP - 40
PB - Science Publishing Group
SN - 2472-3576
UR - https://doi.org/10.11648/j.jddmc.20220803.11
AB - Citrus medica is very popular in traditional system of medicine specially in China and India. The fruit possesses various medicinal activities. Many studies have been conducted on Citrus medica to authenticate its use as multipurpose medicinal agent. Aim of current study was to standardize the fruit of Citrus medica followed by evaluation of anti-urolithiasis activity and preparation of formulation. Flavonoid rich extract of Citrus medicawas prepared by maceration followed by liquid liquid extraction. Qualitative analysis was carried by TLC. Extract was then subjected to spray drying process to get free following powder for formulation. Standardization of flavonoid rich extract of fruit of CM was carried out by HPTLC method using toluene: ethyl acetate: formic acid (6:4:0.1 v/v) asmobile phaseand Silica gel GF 254nm as stationary phase with biomarker i.e. naringenin. Detection was carried out at 281 nm. Total flavonoid content was estimated by UV spectroscopy by considering quercetin as standard. Anti-urolithiasis activity was evaluated by in-vitro model using cystone as standard agent used for kidney stone. Extract showed potent activity as compared to standard. Spray dried powder of flavonoid rich extract was then incorporated in tablet formulation. Evaluation of tablet was carried out as per guidelines and it passes as per the specifications. Hence Citrus medica fruits which are traditionally popular is proved as potent agent as well as standardization give assurity about quality of the herbal formulation and can be used as potent anti-urolithiatic agent in ayurvedic era.
VL - 8
IS - 3
ER -

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Author Information

Choudhary Dimple

Saraswathi Vidya Bhavan’s College of Pharmacy, Dombivli, India
Naik Archana

Saraswathi Vidya Bhavan’s College of Pharmacy, Dombivli, India

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APA Style

Choudhary Dimple, Naik Archana. (2022). Standardization, Optimization of Formulation and In-vitro Evaluation of Anti-urolithiasis Activity of Citrus medica Extract. Journal of Drug Design and Medicinal Chemistry, 8(3), 34-40. https://doi.org/10.11648/j.jddmc.20220803.11

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ACS Style

Choudhary Dimple; Naik Archana. Standardization, Optimization of Formulation and In-vitro Evaluation of Anti-urolithiasis Activity of Citrus medica Extract. J. Drug Des. Med. Chem. 2022, 8(3), 34-40. doi: 10.11648/j.jddmc.20220803.11

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AMA Style

Choudhary Dimple, Naik Archana. Standardization, Optimization of Formulation and In-vitro Evaluation of Anti-urolithiasis Activity of Citrus medica Extract. J Drug Des Med Chem. 2022;8(3):34-40. doi: 10.11648/j.jddmc.20220803.11

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@article{10.11648/j.jddmc.20220803.11,
  author = {Choudhary Dimple and Naik Archana},
  title = {Standardization, Optimization of Formulation and In-vitro Evaluation of Anti-urolithiasis Activity of Citrus medica Extract},
  journal = {Journal of Drug Design and Medicinal Chemistry},
  volume = {8},
  number = {3},
  pages = {34-40},
  doi = {10.11648/j.jddmc.20220803.11},
  url = {https://doi.org/10.11648/j.jddmc.20220803.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jddmc.20220803.11},
  abstract = {Citrus medica is very popular in traditional system of medicine specially in China and India. The fruit possesses various medicinal activities. Many studies have been conducted on Citrus medica to authenticate its use as multipurpose medicinal agent. Aim of current study was to standardize the fruit of Citrus medica followed by evaluation of anti-urolithiasis activity and preparation of formulation. Flavonoid rich extract of Citrus medicawas prepared by maceration followed by liquid liquid extraction. Qualitative analysis was carried by TLC. Extract was then subjected to spray drying process to get free following powder for formulation. Standardization of flavonoid rich extract of fruit of CM was carried out by HPTLC method using toluene: ethyl acetate: formic acid (6:4:0.1 v/v) asmobile phaseand Silica gel GF 254nm as stationary phase with biomarker i.e. naringenin. Detection was carried out at 281 nm. Total flavonoid content was estimated by UV spectroscopy by considering quercetin as standard. Anti-urolithiasis activity was evaluated by in-vitro model using cystone as standard agent used for kidney stone. Extract showed potent activity as compared to standard. Spray dried powder of flavonoid rich extract was then incorporated in tablet formulation. Evaluation of tablet was carried out as per guidelines and it passes as per the specifications. Hence Citrus medica fruits which are traditionally popular is proved as potent agent as well as standardization give assurity about quality of the herbal formulation and can be used as potent anti-urolithiatic agent in ayurvedic era.},
 year = {2022}
}

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