Pimpinella anisum L. (family Apiaceae) is widely used for curing variety of ailments. The objective of this study was to evaluate the antimicrobial activity and chemical composition of chloroform and ethanol extracts from the seed of Pimpinella anisum against standard microorganism. This plant has been used as a traditional treatment for several diseases such as microbial infections. Extracts were evaluated for their effectiveness against four bacterial strains including both Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria as well as fungal species (Candida albicans and Aspergillus niger) using disc diffusion method. The chloroform extract showed the highest activity against B.subtilis (11 mm), E. coli (16mm) and P. aeruginosa (14.5 mm) but the same result against S. aureus in two extracts (15.5 mm). All extracts exhibited high antifungal activity against C. albicans and A. niger with inhibition zone ranging from (14.5 to 20 mm). The quantitative analysis of chemical composition determined by Gas Chromatography–Mass Spectrometry (GC-MS). The result showed high amounts of Butanoic acid, 2-methyl-, 2-methoxy-4-(2-propenyl) phenyl ester (23.21%) and Anethole (12.52%).
| Published in | Journal of Drug Design and Medicinal Chemistry (Volume 4, Issue 2) |
| DOI | 10.11648/j.jddmc.20180402.12 |
| Page(s) | 16-21 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
Pimpinella anisum Seed, Antimicrobial Activity, GC-MS, Chemical Composition, Anethole
| [1] | Ross, I. A., 2001. Medicinal plants of the world: chemical constitutes, traditional and modern medicinal uses, Volume 2. Humana press, Totowa, New Jersey, pp. 363-374. |
| [2] | Tirapelli C, R., Andrade, C. R., de Cassano, A. O., de Souza , F. A, Ambrosio, S. R., Costa , F. B., da Oliveria, A. M. 2007. Antispasmodic and relaxant effects of the hydroalcoholic extract of Pimpinella anisum (Apiaceae) on rat anococcygeous smooth muscle. J Enthopharmacol. 110 (1): 23-29. |
| [3] | Yalcin, S., 1988. Turkey in production and foreign marketing opportunities essential oils, Ankara, IGEME. |
| [4] | Bown, D., 2001. Encyclopedia of Herbs and their Uses. The Herb Society of America, Darling, Kindersley, London. |
| [5] | Kerydiyyeh, S., Usta, J., Kino, K., Markossian, S., Dagher, S., 2003. Aniseed oil increases glucose absorption and reduce urine output in the rat. Life Sci. 74:663-673. |
| [6] | Buchgraber, K., Frühwirt, P., Köppl, P., Krautzer, B., 1997. Produktionsnischen im Pflanzenbau Ginseng, Kümmel, Hanf& Co. Leopold stocker Verlag, Gras- Stuttgart, pp. 35-37. |
| [7] | Ozguven, M., 2001. Aniseed (Pimpinella anisumL.). Handbook of herbs and spices. Peter K V (Ed). Woodhead publishing Limited, Cambridge, England and CRC press. Boca Raton. Boston, New York, Washington DC. |
| [8] | Ozguven, M., Sekin, S., Gurbuz, B., Sekeroglu, N., Ayanoglu, F., Ekren, S., 2005. Tobacco, medicinal and aromatic production and trade. (In) Proceedings of sixth Technical Congress of Turkish Agricultural Engineers, held during 3-7 January at Ankara turkey. 1: 481-501. |
| [9] | Tabanca, N., Demirci, B., Kirimer, N., Baser, K. H. C., Bedir, E., Khan, I. A., Wedge, D. E., 2005. Gas chromatographic - mass spectrometric analysis of essential oil from Pimpinella aurea, Pimpinella corymbosa, Pimpinella peregrinaand Pimpinella puberula gathered from Eastern and Southern Turkey. J. Chromatogr. A 1097: 192-198. |
| [10] | Lawrence D. M., 1984. Progress in essential oils (Anise oil, Calamus oil, Cardamom oils). Perfumer and Flavorist. 8: 63-65. |
| [11] | Askari, F., Sefidkon, F., Mirza, M., 1998. Quantitative and Qualitative of Essential oil Pimpinella anisum. Research and Reconstruction. 38: 70-73. |
| [12] | Omidbaigi, R., Hadjiakhoondi, A., Saharkhiz, M., 2003. Changes in content and chemical composition of Pimpinella anisumL. oil at various harvest time. Journal of Essential oil Bearing Plants. 6 (1): 46-50. |
| [13] | Tabanca, N., Demirci, B., Kirimer, N., Baser, K. H. C., Bedir, E., Khan, I. A., Wedge, D. E., 2006. Gas chromatographic-Mass spectrometric analysis of essential oil from Pimpinella species gathered from Central and Northern turkey. J. Chromatogr. A 1117: 194-205. |
| [14] | Rapisarda, A., 2004. Economic importance and market trends of the genera Pimpinella, Illcium and Foeniculum. Jodral, M. (Ed): Illicum and Foeniculum. Boca Raton: CRC Press, pp. 191-218. |
| [15] | Reineccius, G., 1994. Source book of flavours. 2nd ed. Chapman and Hall. NewYork. |
| [16] | Hänsel, R., Sticher, O., Steinegger, E., 1999. Pharmakognosie-phytopharmaize. 6. Auflage. Springer-Verlag, Berlin, Heidelberg. pp. 692-695. |
| [17] | Omidbaigi, R., 2000. Production and Processing of Medicinal Plants. 3: 26-36. |
| [18] | Zehtab-Salmasi S, Javanshir A, Omidbaigi R, Alyari H and Ghassemigolezani, K. 2001. Effects of water supply and planting date on performance and essential oil production of anis (Pimpinella anisum L.). Act. Agro. Hun. 49(1):75-81. |
| [19] | Awad, N. M., Turky, A. S., Mazhar, A. A., 2005. Effects of bio- and chemical nitrogenous fertilizers on yield of anise (Pimpinella anisumL.) and biological activities of soil irrigated with agricultural drainage water. Egypt. J. Soil. Sci. 45 (3): 265-278. |
| [20] | Tuncturk, M., Yildirim, B., 2006. Effect of seed rates on yield and yield components of anise (Pimpinella anisumL.). Indian J. Agric. Sci. 76 (11): 679-681. |
| [21] | Rechinger, K. H., 1972. Family Umbelliferae. In Flora Iranica Akademische Druck-u. Verlagsanstalt, Graz, Austria, No. 162. |
| [22] | Heywood, V. H., 1999. The biology and chemistry of the Apiaceae. The Linnean Society of London, London. |
| [23] | Rajeshwari, C. U., Abirami, M., Andallu B., 2011. In vitro and in vivo antioxidant potential of aniseed (Pimpinella anisumL.). AsianJ. Exp. Biol. Sci. 2(1): 80-89. |
| [24] | Chevallier, A., 1996. The encyclopedia of medicinal plants. Wolfe Publishing Ltd., London |
| [25] | Figueiredo, A. C., Barroso, J. G., Pedro, L. G., Scheffer, J. J. C., 2008. Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance journal. 23: 213-226. |
| [26] | Rasoanaivo, P. and Ratsimamanga-urveg, S. 1993. Biological evaluation of plants with reference to the Malagasy flora. Monograph of the IFS-NAPRECA workshop on Bioassays, Antanarivo, Madagascar, pp. 72-79. |
| [27] | Akhtar, A., Deshmukh, A. A., Bhonsle, A. V., Kshirsagar P. M and Kolekar. M. A (2008). In vitro Antibacterial activity of Pimpinella anisum fruit extracts against some pathogenic bacteria, Veterinary World, 1(9): 272-274. |
| [28] | Mohamed. H. S. A. A., Abdelgadir, W. S and Almagboul A. Z. I (2015). In vitro antimicrobial activity of Anise seed (Pimpinella anisum L.). Inter. J. of Adv. Res; 3(1):359-367 |
| [29] | Marinov. V and Valcheva-Kuzmanova. S. 2015. Review on the pharmacological activities Of anethole., Scripta Scientifica Pharmaceutica, 2(2):14-19. |
APA Style
Ayat Ahmed Alrasheid, Babeker Siddig Abdallah, Amar Osman Ali. (2018). In Vitro Antimicrobial Activity and GC-MS Analysis of Seed Extracts from Pimpinella anisum L. Journal of Drug Design and Medicinal Chemistry, 4(2), 16-21. https://doi.org/10.11648/j.jddmc.20180402.12
ACS Style
Ayat Ahmed Alrasheid; Babeker Siddig Abdallah; Amar Osman Ali. In Vitro Antimicrobial Activity and GC-MS Analysis of Seed Extracts from Pimpinella anisum L. J. Drug Des. Med. Chem. 2018, 4(2), 16-21. doi: 10.11648/j.jddmc.20180402.12
AMA Style
Ayat Ahmed Alrasheid, Babeker Siddig Abdallah, Amar Osman Ali. In Vitro Antimicrobial Activity and GC-MS Analysis of Seed Extracts from Pimpinella anisum L. J Drug Des Med Chem. 2018;4(2):16-21. doi: 10.11648/j.jddmc.20180402.12
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Download@article{10.11648/j.jddmc.20180402.12,
author = {Ayat Ahmed Alrasheid and Babeker Siddig Abdallah and Amar Osman Ali},
title = {In Vitro Antimicrobial Activity and GC-MS Analysis of Seed Extracts from Pimpinella anisum L},
journal = {Journal of Drug Design and Medicinal Chemistry},
volume = {4},
number = {2},
pages = {16-21},
doi = {10.11648/j.jddmc.20180402.12},
url = {https://doi.org/10.11648/j.jddmc.20180402.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jddmc.20180402.12},
abstract = {Pimpinella anisum L. (family Apiaceae) is widely used for curing variety of ailments. The objective of this study was to evaluate the antimicrobial activity and chemical composition of chloroform and ethanol extracts from the seed of Pimpinella anisum against standard microorganism. This plant has been used as a traditional treatment for several diseases such as microbial infections. Extracts were evaluated for their effectiveness against four bacterial strains including both Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria as well as fungal species (Candida albicans and Aspergillus niger) using disc diffusion method. The chloroform extract showed the highest activity against B.subtilis (11 mm), E. coli (16mm) and P. aeruginosa (14.5 mm) but the same result against S. aureus in two extracts (15.5 mm). All extracts exhibited high antifungal activity against C. albicans and A. niger with inhibition zone ranging from (14.5 to 20 mm). The quantitative analysis of chemical composition determined by Gas Chromatography–Mass Spectrometry (GC-MS). The result showed high amounts of Butanoic acid, 2-methyl-, 2-methoxy-4-(2-propenyl) phenyl ester (23.21%) and Anethole (12.52%).},
year = {2018}
}
TY - JOUR T1 - In Vitro Antimicrobial Activity and GC-MS Analysis of Seed Extracts from Pimpinella anisum L AU - Ayat Ahmed Alrasheid AU - Babeker Siddig Abdallah AU - Amar Osman Ali Y1 - 2018/07/24 PY - 2018 N1 - https://doi.org/10.11648/j.jddmc.20180402.12 DO - 10.11648/j.jddmc.20180402.12 T2 - Journal of Drug Design and Medicinal Chemistry JF - Journal of Drug Design and Medicinal Chemistry JO - Journal of Drug Design and Medicinal Chemistry SP - 16 EP - 21 PB - Science Publishing Group SN - 2472-3576 UR - https://doi.org/10.11648/j.jddmc.20180402.12 AB - Pimpinella anisum L. (family Apiaceae) is widely used for curing variety of ailments. The objective of this study was to evaluate the antimicrobial activity and chemical composition of chloroform and ethanol extracts from the seed of Pimpinella anisum against standard microorganism. This plant has been used as a traditional treatment for several diseases such as microbial infections. Extracts were evaluated for their effectiveness against four bacterial strains including both Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria as well as fungal species (Candida albicans and Aspergillus niger) using disc diffusion method. The chloroform extract showed the highest activity against B.subtilis (11 mm), E. coli (16mm) and P. aeruginosa (14.5 mm) but the same result against S. aureus in two extracts (15.5 mm). All extracts exhibited high antifungal activity against C. albicans and A. niger with inhibition zone ranging from (14.5 to 20 mm). The quantitative analysis of chemical composition determined by Gas Chromatography–Mass Spectrometry (GC-MS). The result showed high amounts of Butanoic acid, 2-methyl-, 2-methoxy-4-(2-propenyl) phenyl ester (23.21%) and Anethole (12.52%). VL - 4 IS - 2 ER -
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