Investigation of the Effect of Silicon Concentration on Biocompatibility and Bioactivity of Spray Pyrolyzed Bioactive Glass

Tsion Chuni Aklilu; Bethelhem Gashaw Ewnete; Megersa Aboneh Mekuria; Aster Aberra Tessema; Filimon Hadish; Fetene Fufa Bakare

doi:doi:10.11648/j.jb.20230701.11

Research Article |

Investigation of the Effect of Silicon Concentration on Biocompatibility and Bioactivity of Spray Pyrolyzed Bioactive Glass

Tsion Chuni Aklilu, Bethelhem Gashaw Ewnete, Megersa Aboneh Mekuria, Aster Aberra Tessema, Filimon Hadish, Fetene Fufa Bakare^*

Published in Journal of Biomaterials (Volume 7, Issue 1)

Received: 26 October 2023 Accepted: 22 November 2023 Published: 6 December 2023

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Abstract

Bioactive glass is a prominent biomaterial that can bind with both soft and hard tissues when in contact with body fluid. This study aims to find out how silicon concentration affects the cytotoxicity and bioactivity of bioactive glass. The 58S, 68S, and 76S BG particles were synthesized using a spray pyrolysis method. XRD, SEM, FTIR, and BET were used to examine the phase composition, morphology, chemical identity, and specific surface area of BG powders. The ability of the specimens to generate apatite on their surface after being soaked in the simulated body fluid (SBF) was determined using an in vitro test to measure their bioactivity. To evaluate the bioactivity of the BG powders, the in vitro apatite formation was investigated using XRD, FTIR, and SEM. Also, an in vitro cytotoxicity test was done using (MTT assay). A cell-growing environment was used to evaluate the in vitro cytotoxicity test based on different extraction concentrations of glass particles. The experimental results suggested that as silicon concentration in the BG increased, cell viability increased whereas bioactivity was reduced. Finally, the correlation between silicon content and cell viability and bioactivity was explored.

Published in	Journal of Biomaterials (Volume 7, Issue 1)
DOI	10.11648/j.jb.20230701.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Bioactive Glass, Bioactivity, Cytotoxicity, Silicon Concentration, Spray Pyrolysis

References

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APA Style

Chuni Aklilu, T., Gashaw Ewnete, B., Aboneh Mekuria, M., Aberra Tessema, A., Hadish, F., et al. (2023). Investigation of the Effect of Silicon Concentration on Biocompatibility and Bioactivity of Spray Pyrolyzed Bioactive Glass. Journal of Biomaterials, 7(1), 1-7. https://doi.org/10.11648/j.jb.20230701.11

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ACS Style

Chuni Aklilu, T.; Gashaw Ewnete, B.; Aboneh Mekuria, M.; Aberra Tessema, A.; Hadish, F., et al. Investigation of the Effect of Silicon Concentration on Biocompatibility and Bioactivity of Spray Pyrolyzed Bioactive Glass. J. Biomater. 2023, 7(1), 1-7. doi: 10.11648/j.jb.20230701.11

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AMA Style

Chuni Aklilu T, Gashaw Ewnete B, Aboneh Mekuria M, Aberra Tessema A, Hadish F, et al. Investigation of the Effect of Silicon Concentration on Biocompatibility and Bioactivity of Spray Pyrolyzed Bioactive Glass. J Biomater. 2023;7(1):1-7. doi: 10.11648/j.jb.20230701.11

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@article{10.11648/j.jb.20230701.11,
  author = {Tsion Chuni Aklilu and Bethelhem Gashaw Ewnete and Megersa Aboneh Mekuria and Aster Aberra Tessema and Filimon Hadish and Fetene Fufa Bakare},
  title = {Investigation of the Effect of Silicon Concentration on Biocompatibility and Bioactivity of Spray Pyrolyzed Bioactive Glass},
  journal = {Journal of Biomaterials},
  volume = {7},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.jb.20230701.11},
  url = {https://doi.org/10.11648/j.jb.20230701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jb.20230701.11},
  abstract = {Bioactive glass is a prominent biomaterial that can bind with both soft and hard tissues when in contact with body fluid. This study aims to find out how silicon concentration affects the cytotoxicity and bioactivity of bioactive glass. The 58S, 68S, and 76S BG particles were synthesized using a spray pyrolysis method. XRD, SEM, FTIR, and BET were used to examine the phase composition, morphology, chemical identity, and specific surface area of BG powders. The ability of the specimens to generate apatite on their surface after being soaked in the simulated body fluid (SBF) was determined using an in vitro test to measure their bioactivity. To evaluate the bioactivity of the BG powders, the in vitro apatite formation was investigated using XRD, FTIR, and SEM. Also, an in vitro cytotoxicity test was done using (MTT assay). A cell-growing environment was used to evaluate the in vitro cytotoxicity test based on different extraction concentrations of glass particles. The experimental results suggested that as silicon concentration in the BG increased, cell viability increased whereas bioactivity was reduced. Finally, the correlation between silicon content and cell viability and bioactivity was explored.
},
 year = {2023}
}

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TY - JOUR
T1 - Investigation of the Effect of Silicon Concentration on Biocompatibility and Bioactivity of Spray Pyrolyzed Bioactive Glass
AU - Tsion Chuni Aklilu
AU - Bethelhem Gashaw Ewnete
AU - Megersa Aboneh Mekuria
AU - Aster Aberra Tessema
AU - Filimon Hadish
AU - Fetene Fufa Bakare
Y1 - 2023/12/06
PY - 2023
N1 - https://doi.org/10.11648/j.jb.20230701.11
DO - 10.11648/j.jb.20230701.11
T2 - Journal of Biomaterials
JF - Journal of Biomaterials
JO - Journal of Biomaterials
SP - 1
EP - 7
PB - Science Publishing Group
SN - 2640-2629
UR - https://doi.org/10.11648/j.jb.20230701.11
AB - Bioactive glass is a prominent biomaterial that can bind with both soft and hard tissues when in contact with body fluid. This study aims to find out how silicon concentration affects the cytotoxicity and bioactivity of bioactive glass. The 58S, 68S, and 76S BG particles were synthesized using a spray pyrolysis method. XRD, SEM, FTIR, and BET were used to examine the phase composition, morphology, chemical identity, and specific surface area of BG powders. The ability of the specimens to generate apatite on their surface after being soaked in the simulated body fluid (SBF) was determined using an in vitro test to measure their bioactivity. To evaluate the bioactivity of the BG powders, the in vitro apatite formation was investigated using XRD, FTIR, and SEM. Also, an in vitro cytotoxicity test was done using (MTT assay). A cell-growing environment was used to evaluate the in vitro cytotoxicity test based on different extraction concentrations of glass particles. The experimental results suggested that as silicon concentration in the BG increased, cell viability increased whereas bioactivity was reduced. Finally, the correlation between silicon content and cell viability and bioactivity was explored.

VL - 7
IS - 1
ER -

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Author Information

Tsion Chuni Aklilu

Department of Materials Science and Engineering, Adama Science and Technology University, Adama, Ethiopia
Bethelhem Gashaw Ewnete

Department of Materials Science and Engineering, Adama Science and Technology University, Adama, Ethiopia
Megersa Aboneh Mekuria

Animal Biotechnology Directorate, Bio and Emerging Institute Technology, Addis Ababa, Ethiopia
Aster Aberra Tessema

Department of Chemistry, Salale University, Oromia, Ethiopia
Filimon Hadish

Department of Materials Science and Engineering, Adama Science and Technology University, Adama, Ethiopia
Fetene Fufa Bakare

Department of Materials Science and Engineering, Adama Science and Technology University, Adama, Ethiopia; Department of Advanced Materials Science and Engineering Center of Excellence, Adama Science and Technology University, Adama, Ethiopia

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APA Style

Chuni Aklilu, T., Gashaw Ewnete, B., Aboneh Mekuria, M., Aberra Tessema, A., Hadish, F., et al. (2023). Investigation of the Effect of Silicon Concentration on Biocompatibility and Bioactivity of Spray Pyrolyzed Bioactive Glass. Journal of Biomaterials, 7(1), 1-7. https://doi.org/10.11648/j.jb.20230701.11

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ACS Style

Chuni Aklilu, T.; Gashaw Ewnete, B.; Aboneh Mekuria, M.; Aberra Tessema, A.; Hadish, F., et al. Investigation of the Effect of Silicon Concentration on Biocompatibility and Bioactivity of Spray Pyrolyzed Bioactive Glass. J. Biomater. 2023, 7(1), 1-7. doi: 10.11648/j.jb.20230701.11

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AMA Style

Chuni Aklilu T, Gashaw Ewnete B, Aboneh Mekuria M, Aberra Tessema A, Hadish F, et al. Investigation of the Effect of Silicon Concentration on Biocompatibility and Bioactivity of Spray Pyrolyzed Bioactive Glass. J Biomater. 2023;7(1):1-7. doi: 10.11648/j.jb.20230701.11

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@article{10.11648/j.jb.20230701.11,
  author = {Tsion Chuni Aklilu and Bethelhem Gashaw Ewnete and Megersa Aboneh Mekuria and Aster Aberra Tessema and Filimon Hadish and Fetene Fufa Bakare},
  title = {Investigation of the Effect of Silicon Concentration on Biocompatibility and Bioactivity of Spray Pyrolyzed Bioactive Glass},
  journal = {Journal of Biomaterials},
  volume = {7},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.jb.20230701.11},
  url = {https://doi.org/10.11648/j.jb.20230701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jb.20230701.11},
  abstract = {Bioactive glass is a prominent biomaterial that can bind with both soft and hard tissues when in contact with body fluid. This study aims to find out how silicon concentration affects the cytotoxicity and bioactivity of bioactive glass. The 58S, 68S, and 76S BG particles were synthesized using a spray pyrolysis method. XRD, SEM, FTIR, and BET were used to examine the phase composition, morphology, chemical identity, and specific surface area of BG powders. The ability of the specimens to generate apatite on their surface after being soaked in the simulated body fluid (SBF) was determined using an in vitro test to measure their bioactivity. To evaluate the bioactivity of the BG powders, the in vitro apatite formation was investigated using XRD, FTIR, and SEM. Also, an in vitro cytotoxicity test was done using (MTT assay). A cell-growing environment was used to evaluate the in vitro cytotoxicity test based on different extraction concentrations of glass particles. The experimental results suggested that as silicon concentration in the BG increased, cell viability increased whereas bioactivity was reduced. Finally, the correlation between silicon content and cell viability and bioactivity was explored.
},
 year = {2023}
}

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VL - 7
IS - 1
ER -

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