Background: Proparacaine hydrochloride (PH), as a a local anesthetic, is used regularly in ophthalmic surgery and optometry. However, few pieces of research on the ocular surface toxicity of PH eye drops have so far been reported. Purpuse: To evaluate the effect of PH on the ocular surface of mice. Methods: Male C57/BL6 mice were divided into four groups: normal, vehicle-treated, 0.05% PH-treated and 0.5% PH treated 7 days. The clinical indications were tear production, corneal sensitivity, and fluorescein staining. The structure and morphology of the cornea were examined by confocal microscopy; the thickness of the corneal center was examined by anterior segment optical coherence tomography (AS-OCT); and the corneal epithelial microvilli morphology was examined using scanning electron microscopy. Hematoxylin-eosin staining was used to characterize the central corneal morphology. Immunofluorescence staining for cytokeratin 10 (K10) was employed to evaluate squamous metaplasia in the corneal epithelium. Results: The results show that topical PH treatment diminished tear production and corneal sensitivity, and increased corneal fluorescein staining scores. Moreover, PH altered the corneal epithelial microvilli morphology, disrupted the epithelial barrier of the cornea, and promoted apoptosis of ocular surface cells. Furthermore, the expression of K10 in the corneal epithelium was found to be increased. Conclusion: Treatment with 0.5% PH caused instability of the tear film, and changes in corneal sensitivity and ocular surface homeostasis.
| Published in | International Journal of Ophthalmology & Visual Science (Volume 6, Issue 1) |
| DOI | 10.11648/j.ijovs.20210601.17 |
| Page(s) | 46-53 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Proparacaine Hydrochloride, Cornea, Ocular Surface
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APA Style
Xiaofeng Li, Xuemei Wang, Yanzi Wang, Huping Wu, Cheng Li. (2021). Effect of Proparacaine Hydrochloride Eye Drops on the Ocular Surface. International Journal of Ophthalmology & Visual Science, 6(1), 46-53. https://doi.org/10.11648/j.ijovs.20210601.17
ACS Style
Xiaofeng Li; Xuemei Wang; Yanzi Wang; Huping Wu; Cheng Li. Effect of Proparacaine Hydrochloride Eye Drops on the Ocular Surface. Int. J. Ophthalmol. Vis. Sci. 2021, 6(1), 46-53. doi: 10.11648/j.ijovs.20210601.17
AMA Style
Xiaofeng Li, Xuemei Wang, Yanzi Wang, Huping Wu, Cheng Li. Effect of Proparacaine Hydrochloride Eye Drops on the Ocular Surface. Int J Ophthalmol Vis Sci. 2021;6(1):46-53. doi: 10.11648/j.ijovs.20210601.17
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Download@article{10.11648/j.ijovs.20210601.17,
author = {Xiaofeng Li and Xuemei Wang and Yanzi Wang and Huping Wu and Cheng Li},
title = {Effect of Proparacaine Hydrochloride Eye Drops on the Ocular Surface},
journal = {International Journal of Ophthalmology & Visual Science},
volume = {6},
number = {1},
pages = {46-53},
doi = {10.11648/j.ijovs.20210601.17},
url = {https://doi.org/10.11648/j.ijovs.20210601.17},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijovs.20210601.17},
abstract = {Background: Proparacaine hydrochloride (PH), as a a local anesthetic, is used regularly in ophthalmic surgery and optometry. However, few pieces of research on the ocular surface toxicity of PH eye drops have so far been reported. Purpuse: To evaluate the effect of PH on the ocular surface of mice. Methods: Male C57/BL6 mice were divided into four groups: normal, vehicle-treated, 0.05% PH-treated and 0.5% PH treated 7 days. The clinical indications were tear production, corneal sensitivity, and fluorescein staining. The structure and morphology of the cornea were examined by confocal microscopy; the thickness of the corneal center was examined by anterior segment optical coherence tomography (AS-OCT); and the corneal epithelial microvilli morphology was examined using scanning electron microscopy. Hematoxylin-eosin staining was used to characterize the central corneal morphology. Immunofluorescence staining for cytokeratin 10 (K10) was employed to evaluate squamous metaplasia in the corneal epithelium. Results: The results show that topical PH treatment diminished tear production and corneal sensitivity, and increased corneal fluorescein staining scores. Moreover, PH altered the corneal epithelial microvilli morphology, disrupted the epithelial barrier of the cornea, and promoted apoptosis of ocular surface cells. Furthermore, the expression of K10 in the corneal epithelium was found to be increased. Conclusion: Treatment with 0.5% PH caused instability of the tear film, and changes in corneal sensitivity and ocular surface homeostasis.},
year = {2021}
}
TY - JOUR T1 - Effect of Proparacaine Hydrochloride Eye Drops on the Ocular Surface AU - Xiaofeng Li AU - Xuemei Wang AU - Yanzi Wang AU - Huping Wu AU - Cheng Li Y1 - 2021/03/10 PY - 2021 N1 - https://doi.org/10.11648/j.ijovs.20210601.17 DO - 10.11648/j.ijovs.20210601.17 T2 - International Journal of Ophthalmology & Visual Science JF - International Journal of Ophthalmology & Visual Science JO - International Journal of Ophthalmology & Visual Science SP - 46 EP - 53 PB - Science Publishing Group SN - 2637-3858 UR - https://doi.org/10.11648/j.ijovs.20210601.17 AB - Background: Proparacaine hydrochloride (PH), as a a local anesthetic, is used regularly in ophthalmic surgery and optometry. However, few pieces of research on the ocular surface toxicity of PH eye drops have so far been reported. Purpuse: To evaluate the effect of PH on the ocular surface of mice. Methods: Male C57/BL6 mice were divided into four groups: normal, vehicle-treated, 0.05% PH-treated and 0.5% PH treated 7 days. The clinical indications were tear production, corneal sensitivity, and fluorescein staining. The structure and morphology of the cornea were examined by confocal microscopy; the thickness of the corneal center was examined by anterior segment optical coherence tomography (AS-OCT); and the corneal epithelial microvilli morphology was examined using scanning electron microscopy. Hematoxylin-eosin staining was used to characterize the central corneal morphology. Immunofluorescence staining for cytokeratin 10 (K10) was employed to evaluate squamous metaplasia in the corneal epithelium. Results: The results show that topical PH treatment diminished tear production and corneal sensitivity, and increased corneal fluorescein staining scores. Moreover, PH altered the corneal epithelial microvilli morphology, disrupted the epithelial barrier of the cornea, and promoted apoptosis of ocular surface cells. Furthermore, the expression of K10 in the corneal epithelium was found to be increased. Conclusion: Treatment with 0.5% PH caused instability of the tear film, and changes in corneal sensitivity and ocular surface homeostasis. VL - 6 IS - 1 ER -
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