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Lipidomics: New Frontier of the Ketogenic Diet

Received: 15 August 2021     Accepted: 31 August 2021     Published: 10 September 2021
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Abstract

Lipidomics is the discipline that studies lipid changes that occur during cellular metabolism. This new approach can be applied to the ketogenic diet (KD) where fats are the predominant macronutrient. After a few days of reduced carbohydrate consumption, glucose reserves become insufficient both for normal fat oxidation via the supply of oxaloacetate in the Krebs cycle and for the supply of glucose to the central nervous system. The alternative energy source is derived from the overproduction of acetyl coenzyme A. This condition, called ketogenesis, leads to the production of higher-than-normal levels of so-called ketone bodies. The acceleration of the production of monounsaturated fats (MF) is so characteristic of obesity that the palmitic-palmitoleic track is indicated as a biomarker even the risk of weight gain. Palmitoleic is known as lipoquine, as it regulates the fat transfer from adipose tissue to muscle. In obesity it increases because it is a defence mechanism of the body to transport fats to the muscle, thus avoiding their excessive accumulation in the liver. The saturated/MF ratio indicates the level of stiffness of the membrane. The increase in membrane stiffness leads to a decrease in the number of insulin receptors predisposing to the onset of diabetes. Each cellular compartment has its own lipid content which can be monitored by lipidomics but the erythrocyte membrane has been shown to have the suitable characteristics to become an important site for lipidomic analysis. Conclusions: KD could be customized based on the results of the membrane lipidomic analysis. The lipidomic profile of an obese subject is characterized by an imbalance of PUFA in favor of omega-6 and by an excess of MF. This imbalance must be taken into consideration in the formulation of the KD protocol: only in this way, the epigenetic structure will be favorable to the establishment of a new balance unfavorable to fat accumulation.

Published in International Journal of Nutrition and Food Sciences (Volume 10, Issue 5)
DOI 10.11648/j.ijnfs.20211005.11
Page(s) 95-100
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Ketogenic Diet, Lipidomics, Ketone Bodies, Customized Diet

References
[1] Ferreri, C., & Chatgilialoglu C. (2011). Cell and lipidomic membrane. Bologna: National Research Council, ISBN: 9788880801306.
[2] Nelson, D. L., & Cox M. M. (2010). Lehninger Principles of Biochemistry – V° edition. Bologna: Zanichelli, ISBN: 9788808064035.
[3] Paoli, A., Canato M., Toniolo, L., Bargossi, A., Neri, M., Mediati, M., Alesso, D., Sanna, G., Grimaldi, K. A., Fazzari, A. L., Bianco, A. (2011). The ketogenic diet: an underappreciated therapeutic option? Clin Ter, 162, (5): e137-146.
[4] Cahill, G. F. Jr. (1970). Starvation in man. The New England Journal of Medicine, Mar 19; 282 (12): 668-75.
[5] Cantrell, C. B., Mohiuddin, S. S. (2020). Biochemistry, Ketone Metabolism. StatPearls. Treasure Island (FL): StatPearls Publishing LLC.
[6] Ota, M., Matsuo, J., Ishida, I., Hattori, K., Teraishi, T., Tonouchi, H., Ashidda, K., TakahasiT., Kunughi, H. (2016). Effect of a ketogenic meal on cognitive function in elderly adults: potential for cognitive enhancement. Psychopharmacology 233, 3797–3802.
[7] Newman, J. C. & Verdin, E. (2014). Ketone bodies as signaling metabolites. Trends in Endocrinology & Metabolism 25 (1): 42-52.
[8] Jensen, N. J., Wodschow, H. Z., Nilsson, M., Rungby, J. (2020). Effects of Ketone Bodies on Brain Metabolism and Function in Neurodegenerative Diseases. International Journal of Molecular Sciences 21 (22): 8767.
[9] Elizondo, A., Araya, J., Nilsson, M., Rodrigo, R., Poniachik, J., Csendes, A., Maluenda, F., Diaz, J. C., Signorini, C., Sgherri, C., Comporti, M., Videla, L. A., (2007). Polyunsaturated fatty acid pattern in liver and erythrocyte phospholipids from obese patient. Obesity Jan; 15 (1): 24-31.
[10] Warensjo, E., Ohrvall, M., Vessby, B., (2006). Fatty acid composition and estimated desaturase activities are associated with obesity and lifestyle variables in men and women. Nutr Metab Cardiovasc Dis 2006 Mar; 16 (2): 128-36.
[11] Sansone, A., Tolika, E., Louka, M., Sunda, V., Deplano, S., Melchiorre, M., Anagnostopoulos, D., Chatgilialoglu, C., Formisano, C., Di Micco, R., Faraone-Mennella, M. R., Ferreri, C., (2016). Hexadecenoic Fatty Acid Isomers in Human Blood Lipids and Their Relevance for the Interpretation of Lipidomic Profiles PLoS One Apr 5; 11 (4): e0152378.
[12] Zhou, H., Urso, C. J., Jadeja, V., (2020). Saturated Fatty Acids in Obesity-Associated Inflammation. J Inflamm Res Jan 6; 13: 1-14.
[13] Saini, R. K. & Keum, Y., (2018). Omega-3 and omega-6 polyunsaturated fatty acids: dietary sources, metabolism and significance – A review. Life Sci Jun 15; 203: 255-267.
[14] Paoli, A., Mancin, L., Bianco, A., Thomas, E., Mota, J. F., Piccini, F., (2019). Ketogenic Diet and Microbiota: Friends or Enemies? Genes (Basel) Jul 15; 10 (7): 534.
[15] Amézaga, J., Ugartemendia, G., Larraioz, A., Bretana, N., Iruretagoyena, A., Camba, J., Urruticoechea, A., Ferreri, C., Tueros, I., (2020). Altered Levels of Desaturation and ω-6 Fatty Acids in Breast Cancer Patients' Red Blood Cell Membranes. Metabolites 10 (11): 469.
[16] Acar, N., Berdeaux, O., Grégoire, S., Cabaret, S., Martine, L., Gain, P., Thuret, G., Creuzot-Garcher, C. P., Bron, A. M., Bretillon, L., (2012). Lipid composition of the human eye: are red blood cells a good mirror of retinal and optic nerve fatty acids? PLoS One 7 (4): e35102.
[17] Jauregibeitia, I., Portune, K., Rica, I., Tueros, I., Velasco, O., Grau, G., Trebolazabala, N., Castaño, L., Larocca, A. V., Ferreri, C., Arranz, S., (2020). Fatty Acid Profile of Mature Red Blood Cell Membranes and Dietary Intake as a New Approach to Characterize Children with Overweight and Obesity. Nutrients Nov 10; 12 (11): 3446.
[18] Baur, L. A., O’Connor, J., Pan, D. A., Wu, B. J., O’Connor, M. J., Storlien, L. H., (2000). Relationships between the fatty acid composition of muscle and erythrocyte membrane phospholipid in young children and the effect of type of infant feeding. Lipids. 35: 77–82.
[19] Heude, B., Ducimetière, P., Berr, C., EVA Study, (2003). Cognitive decline and fatty acid composition of erythrocyte membranes – the EVA study. American Journal Clinic Nutrition 77: 803–8.
[20] Fan, R., Toney, A. M., Jang, Y., Ro, S. H., Chung. S., (2018). Maternal n-3 PUFA supplementation promotes fetal brown adipose tissue development through epigenetic modifications in C57BL/6 mice. Biochim Biophys Acta Mol Cell Biol Lipids 1863 (12): 1488-1497.
[21] Triff, K., Kim, E., Chapkin, R. S., (2015). Chemoprotective epigenetic mechanisms in a colorectal cancer model: Modulation by n-3 PUFA in combination with fermentable fiber. Curr Pharmacol Rep 1 (1): 11-20.
[22] Jump, D. B., Botolin, D., Wang, Y., Xu, J., Demeure, O., Christian, B., (2008). Docosahexaenoic acid (DHA) and hepatic gene transcription. Chem Phys Lipids 153 (1): 3-13.
[23] Willett, W. C., (2002) Dietary fat plays a major role in obesity: no. Obesity reviews: an official journal of the International Association for the Study of Obesity, 3 (2): 59-68.
[24] Ailhaud, G., Masseira, F., Alessandri, J. M., Guesnet, P., (2007). Fatty acid composition as an early determinant of childhood obesity. Genes & nutrition, 2 (1): 39-40.
[25] Simopoulous, A. P., (1999). Essential fatty acids in health and chronic disease. Am J Clin Nutr, 70 (3S): 560s-569s.
[26] Caprio, M., Infante, M., Moriconi, E., Armani, A., Fabbri, A., Mantovani, G., Mariani, S., Lubrano, C., Poggiogalle, E., Migliaccio, S., Donini, L. M., Basciani, S., cignarelli, A, Conte, E., Ceccarini, G., Bogazzi, F., Cimino, L., Condorelli, R. A., La vignera, S., Calogero, A. E., Gamberini, A., Vignozzi, L., Prodam, F., Aimaretti, G., Linsalata, G. Buralli, S., Monzani, F., Aversa, A., Vettor, R., Santini, F., Vitti, P., Gnessi, L., Pagotto, U., Giorgino, F., Colao, A., Lenzi, A., Cardiovascular Endocrinology Club of the Italian Society of Endocrinology, (2019). Very-low-calorie ketogenic diet (VLCKD) in the management of metabolic diseases: systematic review and consensus statement from the Italian Society of Endocrinology (SIE). J Endocrinol Invest 42 (11): 1365-1386.
[27] CUNNANE, S. C. Metabolism of polyunsaturated fatty acids and ketogenesis: an emerging connection. Prostaglandins Leukot Essent Fatty Acids, 70, n. 3, p. 237- 241, Mar 2004.
[28] HENNEBELLE, M.; COURCHESNE-LOYER, A.; ST-PIERRE, V.; VANDENBERGHE, C. et al. Preliminary evaluation of a differential effect of an alpha-linolenate-rich supplement on ketogenesis and plasma omega-3 fatty acids in young and older adults. Nutrition, 32, n. 11-12, p. 1211-1216, Nov-Dec 2016.
[29] Paoli, Moro et al., Effects of n-3 Polyunsaturated Fatty Acids (ω-3) Supplementation on Some Cardiovascular Risk Factors with a Ketogenic Mediterranean Diet. Marine Drugs, 2015.
[30] Volek JS, Gómez AL, Kraemer WJ. Fasting lipoprotein and postprandial triacylglycerol responses to a low-carbohydrate diet supplemented with n-3 fatty acids. J Am Coll Nutr. 2000 Jun; 19 (3): 383-91. doi: 10.1080/07315724.2000.10718935. PMID: 10872901.
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  • APA Style

    Alessandra Lodi, Lorenzo Cenci. (2021). Lipidomics: New Frontier of the Ketogenic Diet. International Journal of Nutrition and Food Sciences, 10(5), 95-100. https://doi.org/10.11648/j.ijnfs.20211005.11

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    ACS Style

    Alessandra Lodi; Lorenzo Cenci. Lipidomics: New Frontier of the Ketogenic Diet. Int. J. Nutr. Food Sci. 2021, 10(5), 95-100. doi: 10.11648/j.ijnfs.20211005.11

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    AMA Style

    Alessandra Lodi, Lorenzo Cenci. Lipidomics: New Frontier of the Ketogenic Diet. Int J Nutr Food Sci. 2021;10(5):95-100. doi: 10.11648/j.ijnfs.20211005.11

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  • @article{10.11648/j.ijnfs.20211005.11,
      author = {Alessandra Lodi and Lorenzo Cenci},
      title = {Lipidomics: New Frontier of the Ketogenic Diet},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {10},
      number = {5},
      pages = {95-100},
      doi = {10.11648/j.ijnfs.20211005.11},
      url = {https://doi.org/10.11648/j.ijnfs.20211005.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20211005.11},
      abstract = {Lipidomics is the discipline that studies lipid changes that occur during cellular metabolism. This new approach can be applied to the ketogenic diet (KD) where fats are the predominant macronutrient. After a few days of reduced carbohydrate consumption, glucose reserves become insufficient both for normal fat oxidation via the supply of oxaloacetate in the Krebs cycle and for the supply of glucose to the central nervous system. The alternative energy source is derived from the overproduction of acetyl coenzyme A. This condition, called ketogenesis, leads to the production of higher-than-normal levels of so-called ketone bodies. The acceleration of the production of monounsaturated fats (MF) is so characteristic of obesity that the palmitic-palmitoleic track is indicated as a biomarker even the risk of weight gain. Palmitoleic is known as lipoquine, as it regulates the fat transfer from adipose tissue to muscle. In obesity it increases because it is a defence mechanism of the body to transport fats to the muscle, thus avoiding their excessive accumulation in the liver. The saturated/MF ratio indicates the level of stiffness of the membrane. The increase in membrane stiffness leads to a decrease in the number of insulin receptors predisposing to the onset of diabetes. Each cellular compartment has its own lipid content which can be monitored by lipidomics but the erythrocyte membrane has been shown to have the suitable characteristics to become an important site for lipidomic analysis. Conclusions: KD could be customized based on the results of the membrane lipidomic analysis. The lipidomic profile of an obese subject is characterized by an imbalance of PUFA in favor of omega-6 and by an excess of MF. This imbalance must be taken into consideration in the formulation of the KD protocol: only in this way, the epigenetic structure will be favorable to the establishment of a new balance unfavorable to fat accumulation.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Lipidomics: New Frontier of the Ketogenic Diet
    AU  - Alessandra Lodi
    AU  - Lorenzo Cenci
    Y1  - 2021/09/10
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ijnfs.20211005.11
    DO  - 10.11648/j.ijnfs.20211005.11
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
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    AB  - Lipidomics is the discipline that studies lipid changes that occur during cellular metabolism. This new approach can be applied to the ketogenic diet (KD) where fats are the predominant macronutrient. After a few days of reduced carbohydrate consumption, glucose reserves become insufficient both for normal fat oxidation via the supply of oxaloacetate in the Krebs cycle and for the supply of glucose to the central nervous system. The alternative energy source is derived from the overproduction of acetyl coenzyme A. This condition, called ketogenesis, leads to the production of higher-than-normal levels of so-called ketone bodies. The acceleration of the production of monounsaturated fats (MF) is so characteristic of obesity that the palmitic-palmitoleic track is indicated as a biomarker even the risk of weight gain. Palmitoleic is known as lipoquine, as it regulates the fat transfer from adipose tissue to muscle. In obesity it increases because it is a defence mechanism of the body to transport fats to the muscle, thus avoiding their excessive accumulation in the liver. The saturated/MF ratio indicates the level of stiffness of the membrane. The increase in membrane stiffness leads to a decrease in the number of insulin receptors predisposing to the onset of diabetes. Each cellular compartment has its own lipid content which can be monitored by lipidomics but the erythrocyte membrane has been shown to have the suitable characteristics to become an important site for lipidomic analysis. Conclusions: KD could be customized based on the results of the membrane lipidomic analysis. The lipidomic profile of an obese subject is characterized by an imbalance of PUFA in favor of omega-6 and by an excess of MF. This imbalance must be taken into consideration in the formulation of the KD protocol: only in this way, the epigenetic structure will be favorable to the establishment of a new balance unfavorable to fat accumulation.
    VL  - 10
    IS  - 5
    ER  - 

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Author Information
  • Nutrition Department, Kerix Lab, Vicenza, Italy

  • Department of Biomedical Sciences, University of Padova, Padova, Italy

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