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Protective Effect of Grape Seed Oil on Hypercholesterolemia in Rats

Received: 3 July 2016     Accepted: 13 July 2016     Published: 4 August 2016
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Abstract

This study was carried out to investigate that the effect of grape seed oil (GSO) on Hypercholesterolemia in rats. Thirty-five rats were divided into 5 groups, group 1 was given the basal diet as a negative control group (-ve), group 2 was given the basal diet with GSO instead of soybean oil as a negative control for GSO. Groups (3, 4 and 5) were fed on high cholesterol diet (HCD) (1% cholesterol powder and 0.5% bile salt for 8 weeks), Group 3 was +ve control, group 4 and 5 were treatment groups which received GSO 2% and 4% daily with HCD. The results of this study indicated that the GSO caused an improvement in the blood lipids especially 4% GSO. The 4% GSO decreased serum TC, TG, LDL-c and VLDL significantly (P ˂ 0.05), and caused a significant increase in HDL-C level. Also, 2% GSO decreased TG, LDL-C and VLDL significantly (P ˂ 0.05), whereas the level of HDL-C showed significant increases. Subsequently, GSO enhanced the lipid ratios: atherogenic coefficient (AC), cardiac risk ratio (CRR), LDL-c to HDL-c ratio and atherogenic index of plasma (AIP). Moreover, the serum, liver function (AST, ALT and ALP) levels also, enhanced in the GSO groups. However, 4% GSO led to significant decrease in serum MDA and elevated serum GST. Otherwise, histopathological examination showed enhanced in the heart and aorta of rats compared with the +ve control group. This study indicates that GSO effective in lowering total cholesterol, triglyceride and LDL-c and increasing HDL-c. Therefore, GSO have hypocholesterolemic effect and might be effective to protect against the risk of CVD.

Published in International Journal of Nutrition and Food Sciences (Volume 5, Issue 5)
DOI 10.11648/j.ijnfs.20160505.11
Page(s) 310-317
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Grape Seed Oil, GSO, Lipid Profile, Cholesterol, Triglyceride, HDL

References
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  • APA Style

    Reda Ahmed Mokhtar, Nefisa Hassan El-Banna, Mohammed Hamdy Haggag. (2016). Protective Effect of Grape Seed Oil on Hypercholesterolemia in Rats. International Journal of Nutrition and Food Sciences, 5(5), 310-317. https://doi.org/10.11648/j.ijnfs.20160505.11

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    ACS Style

    Reda Ahmed Mokhtar; Nefisa Hassan El-Banna; Mohammed Hamdy Haggag. Protective Effect of Grape Seed Oil on Hypercholesterolemia in Rats. Int. J. Nutr. Food Sci. 2016, 5(5), 310-317. doi: 10.11648/j.ijnfs.20160505.11

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    AMA Style

    Reda Ahmed Mokhtar, Nefisa Hassan El-Banna, Mohammed Hamdy Haggag. Protective Effect of Grape Seed Oil on Hypercholesterolemia in Rats. Int J Nutr Food Sci. 2016;5(5):310-317. doi: 10.11648/j.ijnfs.20160505.11

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  • @article{10.11648/j.ijnfs.20160505.11,
      author = {Reda Ahmed Mokhtar and Nefisa Hassan El-Banna and Mohammed Hamdy Haggag},
      title = {Protective Effect of Grape Seed Oil on Hypercholesterolemia in Rats},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {5},
      number = {5},
      pages = {310-317},
      doi = {10.11648/j.ijnfs.20160505.11},
      url = {https://doi.org/10.11648/j.ijnfs.20160505.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20160505.11},
      abstract = {This study was carried out to investigate that the effect of grape seed oil (GSO) on Hypercholesterolemia in rats. Thirty-five rats were divided into 5 groups, group 1 was given the basal diet as a negative control group (-ve), group 2 was given the basal diet with GSO instead of soybean oil as a negative control for GSO. Groups (3, 4 and 5) were fed on high cholesterol diet (HCD) (1% cholesterol powder and 0.5% bile salt for 8 weeks), Group 3 was +ve control, group 4 and 5 were treatment groups which received GSO 2% and 4% daily with HCD. The results of this study indicated that the GSO caused an improvement in the blood lipids especially 4% GSO. The 4% GSO decreased serum TC, TG, LDL-c and VLDL significantly (P ˂ 0.05), and caused a significant increase in HDL-C level. Also, 2% GSO decreased TG, LDL-C and VLDL significantly (P ˂ 0.05), whereas the level of HDL-C showed significant increases. Subsequently, GSO enhanced the lipid ratios: atherogenic coefficient (AC), cardiac risk ratio (CRR), LDL-c to HDL-c ratio and atherogenic index of plasma (AIP). Moreover, the serum, liver function (AST, ALT and ALP) levels also, enhanced in the GSO groups. However, 4% GSO led to significant decrease in serum MDA and elevated serum GST. Otherwise, histopathological examination showed enhanced in the heart and aorta of rats compared with the +ve control group. This study indicates that GSO effective in lowering total cholesterol, triglyceride and LDL-c and increasing HDL-c. Therefore, GSO have hypocholesterolemic effect and might be effective to protect against the risk of CVD.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Protective Effect of Grape Seed Oil on Hypercholesterolemia in Rats
    AU  - Reda Ahmed Mokhtar
    AU  - Nefisa Hassan El-Banna
    AU  - Mohammed Hamdy Haggag
    Y1  - 2016/08/04
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ijnfs.20160505.11
    DO  - 10.11648/j.ijnfs.20160505.11
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 310
    EP  - 317
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20160505.11
    AB  - This study was carried out to investigate that the effect of grape seed oil (GSO) on Hypercholesterolemia in rats. Thirty-five rats were divided into 5 groups, group 1 was given the basal diet as a negative control group (-ve), group 2 was given the basal diet with GSO instead of soybean oil as a negative control for GSO. Groups (3, 4 and 5) were fed on high cholesterol diet (HCD) (1% cholesterol powder and 0.5% bile salt for 8 weeks), Group 3 was +ve control, group 4 and 5 were treatment groups which received GSO 2% and 4% daily with HCD. The results of this study indicated that the GSO caused an improvement in the blood lipids especially 4% GSO. The 4% GSO decreased serum TC, TG, LDL-c and VLDL significantly (P ˂ 0.05), and caused a significant increase in HDL-C level. Also, 2% GSO decreased TG, LDL-C and VLDL significantly (P ˂ 0.05), whereas the level of HDL-C showed significant increases. Subsequently, GSO enhanced the lipid ratios: atherogenic coefficient (AC), cardiac risk ratio (CRR), LDL-c to HDL-c ratio and atherogenic index of plasma (AIP). Moreover, the serum, liver function (AST, ALT and ALP) levels also, enhanced in the GSO groups. However, 4% GSO led to significant decrease in serum MDA and elevated serum GST. Otherwise, histopathological examination showed enhanced in the heart and aorta of rats compared with the +ve control group. This study indicates that GSO effective in lowering total cholesterol, triglyceride and LDL-c and increasing HDL-c. Therefore, GSO have hypocholesterolemic effect and might be effective to protect against the risk of CVD.
    VL  - 5
    IS  - 5
    ER  - 

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Author Information
  • Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Cairo, Egypt

  • Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Cairo, Egypt

  • Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Cairo, Egypt

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