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Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction

Received: 1 April 2014     Accepted: 20 April 2014     Published: 30 April 2014
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Abstract

This study was carried out to evaluatea PCR-based method for detection of DNA in cow milk. It utilized primers targeting the mitochondrial cytochrome-b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. For the specific identification of cow mtcyt-b gene, a pair of primers (CSL1, CSR2), which produced a 386 bp PCR product from milk samples as well as from peripheral blood, were used. Amplification products were visualized on ethidium bromide-stained agarose gels. Amplification products were not detected when the PCR was applied to DNA from different animal species including sheep, camel, deerand human, indicating that the 2 pairs of primers are bovine specific. In conclusion, the PCR-based assay used in this study allowed sensitive and specific identification of cow milk DNA.

Published in International Journal of Nutrition and Food Sciences (Volume 3, Issue 3)
DOI 10.11648/j.ijnfs.20140303.12
Page(s) 141-144
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Cytochrome-b, Cow Milk, PCR

References
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[14] Maskova, E. &Paulickova, I. (2006). PCR-based detection of cow's milk in goat and sheep cheese marketed in the Czech Republic. Czech J Food Sci, 24, 127-132.
[15] Abdel-Rahman, S. M.& Ahmed, M. M. M. (2007). Rapid and sensitive identification of buffalo's, cattle's and sheep's milk using species-specific PCR and PCR-RLFP techniques. Food control, 18, 1246-1249.
[16] Kotowicz, M., Adamczyk, E. &Bania, J. (2007).Application of a duplex-PCR for detection of cow's milk in goat's milk. Ann Agric Environ Med, 14, 215-218.
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  • APA Style

    Asim A. Osman, Ayman M. Mahmoud, Ayman S. Soliman. (2014). Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction. International Journal of Nutrition and Food Sciences, 3(3), 141-144. https://doi.org/10.11648/j.ijnfs.20140303.12

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    ACS Style

    Asim A. Osman; Ayman M. Mahmoud; Ayman S. Soliman. Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction. Int. J. Nutr. Food Sci. 2014, 3(3), 141-144. doi: 10.11648/j.ijnfs.20140303.12

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    AMA Style

    Asim A. Osman, Ayman M. Mahmoud, Ayman S. Soliman. Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction. Int J Nutr Food Sci. 2014;3(3):141-144. doi: 10.11648/j.ijnfs.20140303.12

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  • @article{10.11648/j.ijnfs.20140303.12,
      author = {Asim A. Osman and Ayman M. Mahmoud and Ayman S. Soliman},
      title = {Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {3},
      number = {3},
      pages = {141-144},
      doi = {10.11648/j.ijnfs.20140303.12},
      url = {https://doi.org/10.11648/j.ijnfs.20140303.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20140303.12},
      abstract = {This study was carried out to evaluatea PCR-based method for detection of DNA in cow milk. It utilized primers targeting the mitochondrial cytochrome-b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. For the specific identification of cow mtcyt-b gene, a pair of primers (CSL1, CSR2), which produced a 386 bp PCR product from milk samples as well as from peripheral blood, were used. Amplification products were visualized on ethidium bromide-stained agarose gels. Amplification products were not detected when the PCR was applied to DNA from different animal species including sheep, camel, deerand human, indicating that the 2 pairs of primers are bovine specific. In conclusion, the PCR-based assay used in this study allowed sensitive and specific identification of cow milk DNA.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction
    AU  - Asim A. Osman
    AU  - Ayman M. Mahmoud
    AU  - Ayman S. Soliman
    Y1  - 2014/04/30
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ijnfs.20140303.12
    DO  - 10.11648/j.ijnfs.20140303.12
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 141
    EP  - 144
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20140303.12
    AB  - This study was carried out to evaluatea PCR-based method for detection of DNA in cow milk. It utilized primers targeting the mitochondrial cytochrome-b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. For the specific identification of cow mtcyt-b gene, a pair of primers (CSL1, CSR2), which produced a 386 bp PCR product from milk samples as well as from peripheral blood, were used. Amplification products were visualized on ethidium bromide-stained agarose gels. Amplification products were not detected when the PCR was applied to DNA from different animal species including sheep, camel, deerand human, indicating that the 2 pairs of primers are bovine specific. In conclusion, the PCR-based assay used in this study allowed sensitive and specific identification of cow milk DNA.
    VL  - 3
    IS  - 3
    ER  - 

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Author Information
  • Physiology Department, Faculty of Medicine, Zawia University, Libya

  • Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Egypt

  • Physiology Department, Faculty of Medicine, Beni-Suef University, Egypt

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