This study is to explore the expression of CPLX1 (Complexin 1) in spinal cord tissues with different degrees of injury in the subacute phase of spinal cord injury. Twenty-four SD rats were randomly divided into three experimental groups (light, medium and severe injury) and a sham-operated control group. The experimental group made acute spinal cord injury models according to the lisa method, and the spinal cord tissue were collected 72 hours after injury. The tissues of rats were taken for transcriptome high-throughput sequencing and proteomic analysis experiments to observe the expression of CPLX1 in the different degrees during the subacute phase of spinal cord injury. The mRNA and protein of CPLX1 were detect in rat spinal cord tissue, and they were expressed high in the sham operation group. The protein expression in the light injury group began to decrease, and with the increase of the degree of injury, the transcription level of CPLX1 and the amount of protein expression also gradually decreased. The change trend of mRNA transcription level and protein expression level was basically the same. When the spinal cord injury heavier, the less CPLX1 was expressed, the two showed a significant negative correlation. In the subacute stage of spinal cord injury, the translation and protein levels of CPLX1 decreased significantly. The more severe the injury, the lower the transcription level and protein expression level. CPLX1 may be used as a marker gene for the severity of spinal injury.
| Published in | International Journal of Neurosurgery (Volume 4, Issue 2) |
| DOI | 10.11648/j.ijn.20200402.11 |
| Page(s) | 18-21 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2020. Published by Science Publishing Group |
CPLX1, Spinal Cord Injury, Subacute Phase, Degree of Injury
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APA Style
Xinhua Xi, Zhisheng Ji, Yongzheng Bao, Xueren Zhong, Junjian Liao, et al. (2020). Expression of CPLX1 in Different Degrees of Spinal Cord Injury in Subacute Stage. International Journal of Neurosurgery, 4(2), 18-21. https://doi.org/10.11648/j.ijn.20200402.11
ACS Style
Xinhua Xi; Zhisheng Ji; Yongzheng Bao; Xueren Zhong; Junjian Liao, et al. Expression of CPLX1 in Different Degrees of Spinal Cord Injury in Subacute Stage. Int. J. Neurosurg. 2020, 4(2), 18-21. doi: 10.11648/j.ijn.20200402.11
AMA Style
Xinhua Xi, Zhisheng Ji, Yongzheng Bao, Xueren Zhong, Junjian Liao, et al. Expression of CPLX1 in Different Degrees of Spinal Cord Injury in Subacute Stage. Int J Neurosurg. 2020;4(2):18-21. doi: 10.11648/j.ijn.20200402.11
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Download@article{10.11648/j.ijn.20200402.11,
author = {Xinhua Xi and Zhisheng Ji and Yongzheng Bao and Xueren Zhong and Junjian Liao and Qiang Wu and Hongsheng Lin},
title = {Expression of CPLX1 in Different Degrees of Spinal Cord Injury in Subacute Stage},
journal = {International Journal of Neurosurgery},
volume = {4},
number = {2},
pages = {18-21},
doi = {10.11648/j.ijn.20200402.11},
url = {https://doi.org/10.11648/j.ijn.20200402.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijn.20200402.11},
abstract = {This study is to explore the expression of CPLX1 (Complexin 1) in spinal cord tissues with different degrees of injury in the subacute phase of spinal cord injury. Twenty-four SD rats were randomly divided into three experimental groups (light, medium and severe injury) and a sham-operated control group. The experimental group made acute spinal cord injury models according to the lisa method, and the spinal cord tissue were collected 72 hours after injury. The tissues of rats were taken for transcriptome high-throughput sequencing and proteomic analysis experiments to observe the expression of CPLX1 in the different degrees during the subacute phase of spinal cord injury. The mRNA and protein of CPLX1 were detect in rat spinal cord tissue, and they were expressed high in the sham operation group. The protein expression in the light injury group began to decrease, and with the increase of the degree of injury, the transcription level of CPLX1 and the amount of protein expression also gradually decreased. The change trend of mRNA transcription level and protein expression level was basically the same. When the spinal cord injury heavier, the less CPLX1 was expressed, the two showed a significant negative correlation. In the subacute stage of spinal cord injury, the translation and protein levels of CPLX1 decreased significantly. The more severe the injury, the lower the transcription level and protein expression level. CPLX1 may be used as a marker gene for the severity of spinal injury.},
year = {2020}
}
TY - JOUR T1 - Expression of CPLX1 in Different Degrees of Spinal Cord Injury in Subacute Stage AU - Xinhua Xi AU - Zhisheng Ji AU - Yongzheng Bao AU - Xueren Zhong AU - Junjian Liao AU - Qiang Wu AU - Hongsheng Lin Y1 - 2020/07/06 PY - 2020 N1 - https://doi.org/10.11648/j.ijn.20200402.11 DO - 10.11648/j.ijn.20200402.11 T2 - International Journal of Neurosurgery JF - International Journal of Neurosurgery JO - International Journal of Neurosurgery SP - 18 EP - 21 PB - Science Publishing Group SN - 2640-1959 UR - https://doi.org/10.11648/j.ijn.20200402.11 AB - This study is to explore the expression of CPLX1 (Complexin 1) in spinal cord tissues with different degrees of injury in the subacute phase of spinal cord injury. Twenty-four SD rats were randomly divided into three experimental groups (light, medium and severe injury) and a sham-operated control group. The experimental group made acute spinal cord injury models according to the lisa method, and the spinal cord tissue were collected 72 hours after injury. The tissues of rats were taken for transcriptome high-throughput sequencing and proteomic analysis experiments to observe the expression of CPLX1 in the different degrees during the subacute phase of spinal cord injury. The mRNA and protein of CPLX1 were detect in rat spinal cord tissue, and they were expressed high in the sham operation group. The protein expression in the light injury group began to decrease, and with the increase of the degree of injury, the transcription level of CPLX1 and the amount of protein expression also gradually decreased. The change trend of mRNA transcription level and protein expression level was basically the same. When the spinal cord injury heavier, the less CPLX1 was expressed, the two showed a significant negative correlation. In the subacute stage of spinal cord injury, the translation and protein levels of CPLX1 decreased significantly. The more severe the injury, the lower the transcription level and protein expression level. CPLX1 may be used as a marker gene for the severity of spinal injury. VL - 4 IS - 2 ER -
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