Potential Gene Flow Between <i>Gossypium hirsitum</i> and <i>Abelmoschus esculentus</i> Through Interspecific Hybridization

Molo Thierry; Likeng-Li-Ngue Benoit Constant; Ngalle Hermine Bille; Nyouma Achille; Mbo Nkoulou Luther Fort; Eya’a Ngombo Clement; Bell Joseph Martin

doi:doi:10.11648/j.ijgg.20231101.11

Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization

Molo Thierry, Likeng-Li-Ngue Benoit Constant, Ngalle Hermine Bille, Nyouma Achille, Mbo Nkoulou Luther Fort, Eya’a Ngombo Clement, Bell Joseph Martin

Published in International Journal of Genetics and Genomics (Volume 11, Issue 1)

Received: 2 January 2023 Accepted: 25 January 2023 Published: 24 February 2023

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The introduction of transgenic varieties of cotton (Gossypium hirsitum) in Cameroon has posed numerous concerns. One of the most concern was the horizontal transfer of the Bt gene to related species such as Abelmoschus esculentus. The current study was conducted to assess the occurrence Bt gene transfer from Gossypium hirsitum to Abelmoschus esculentus. To achieve this, the stigma receptivity periods and the viability of pollen grains were evaluated. The fruit set rates of 675 controlled intra- and interspecific crosses were assessed. The results showed that stigma receptivity and pollen grain viability are optimal at anthesis in both species, with a longer duration over 24 hours in G. hirsitum. Intra-specific crosses within the two species have shown fruit set rates of nearly 60-80%. Interspecific crosses gave a fruit set rate close to 2%, especially when A. esculentus is used as the female parent, and zero results in reciprocal crosses. The finding of the study pointed out that the possibility of horizontal transfer of a transgene to cotton relatives. In future studies, it would be useful to monitor the fate of seeds from interspecific crosses between both species and assess the agro-morphological and molecular characteristics of these possible hybrids.

Published in	International Journal of Genetics and Genomics (Volume 11, Issue 1)
DOI	10.11648/j.ijgg.20231101.11
Page(s)	1-5
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Interspecific Hybridization, G. hirsitum, A. esculentus, Gene Flow, Trangenic Varieties

References

[1]	Soumare, M., Michel, H., and Bruno, B. (2021). Cotton in West and Central Africa from the agricultural revolution to the agro-ecological transmission. Cahiers Agricultures, 30 (5), 1-8, doi.org/10.1051/cagri/2020044.
[2]	ICAC. (2019). World cotton statistics, December 2019, www.icac.org.
[3]	Planetoscope. (2018). La production mondiale de coton. https://www.planetoscope.com/agriculture-alimentation/1178-production-mondiale-de-coton.html
[4]	SODECOTON. (2014). Note semestriel d’information de la campagne 2013/2014, 1-80.
[5]	Badiane, O., Ghura, D., Goreux, L and Masson, P. (2002). Cotton sector strategies in West and Central Africa. World Bank Policy Research Working 2867, 53-59.
[6]	MINMIDT. (2015). Forum national sur les Organismes Génétiquement Modifiés au Cameroun: Enjeux et défis des OGM pour un développement durable du Cameroun.
[7]	Michaud, D. (2005). Environmental impact of transgenic crops: The impact of recombinant traits. Phytoprotection, 86 (2), 107-124. doi org/10.7202/01251ar.
[8]	Marcel, K and Agnes, R. (2012). Plantes biotechnologiques: Réalité, espoir et obstacle. Futuribles, 383: 73-88.
[9]	Rache, C., Leidy, Y., Mora-Oberleander, J., and Chaparro-Giraldo, A. (2013). Study of gene flow from gm cotton (gossypium hirsutum) varieties in “el espinal”. Acta Biológica Colombiana, 18 (3), 489-498.
[10]	Montes, M., Coriton, O., Eber, F., Huteau, V., Lacape, J. M., Reinhardt, C., Marais, D., Hofs, J. L., Chèvre, A. M and Pannetier, C. (2017). Assessment of gene flow between Gossypium hirsutum and G. herbaceum: Evidence of unreduced gametes in the diploid progenitor. Gene/Genome/Genetics, 7, 2185-2193. doi.org/10.1534/g3.117.041509.
[11]	Athanasios, G. M., Chrysanthi, I. P., Dimitrios, N. V., Ioannis, N. X., Dimitrios, G. R. (2018). Hybridization between cotton and Malvaceae species as a tool for production of partial interspecific aneuploidy cotton plants. Euphytica, 214 (179), 1-13, doi.org/10.1007/s10681-018-2257-5.
[12]	Kearns, C. A and Inouye, D. W. (1993). Techniques for Pollination. Biologists University press of colorado. west Virginia Wesleyan college, Buckhannon, wv26201, union state. 1-1429.
[13]	Alexander, M. P. (1980). Differential staining of aborted and non-aborted pollen. Stain Technology. 55 (1), 13-18.
[14]	Amiteye, S., Amitaaba, T., Akama, C., Harry, M. A. (2019). Hybridization Studies of Okra (Abelmoschus spp. (L.) Moench) Accessions. International Journal of Biotech Trends and Technology, 9 (4), 42-47.
[15]	Avneesh Kumar Singh., Vyakaranahal, B., Deshpande, V., Basavaraj, and Patil, M. (2010). Studies on stigma receptivity, bud size and pollination frequency for hybrid seed production in bhendi (Abelmoschus esculentus (l.) Moench). Karnataka Journal of Agriculture Sciences., 23 (3), 426-430.
[16]	Monika, A and Parihar, A. (2017). Stigma receptivity test in diverse species of tomato. International Journal of Agricultural Science and Research, 7, 1-8.
[17]	Yao, D., Léonie, C., Arsène, I., Zoro, B., Glawdys, Y., and Baudoin, J. (2006.). Study of the botanical, agronomic and floral biology characteristics of the African melon (Cucumis melo L. var. agrestis Naudin, Cucurbitaceae). Biotechnology, Agronomy, Society and Environment, 10 (2), 109-119.
[18]	Parry, G., (1982). The cotton plant and its products. G-P. Maison neuve and larose, Parry (V), 1-474P.

Cite This Article

Plain Text BibTeX RIS

APA Style

Molo Thierry, Likeng-Li-Ngue Benoit Constant, Ngalle Hermine Bille, Nyouma Achille, Mbo Nkoulou Luther Fort, et al. (2023). Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization. International Journal of Genetics and Genomics, 11(1), 1-5. https://doi.org/10.11648/j.ijgg.20231101.11

Copy | Download

ACS Style

Molo Thierry; Likeng-Li-Ngue Benoit Constant; Ngalle Hermine Bille; Nyouma Achille; Mbo Nkoulou Luther Fort, et al. Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization. Int. J. Genet. Genomics 2023, 11(1), 1-5. doi: 10.11648/j.ijgg.20231101.11

Copy | Download

AMA Style

Molo Thierry, Likeng-Li-Ngue Benoit Constant, Ngalle Hermine Bille, Nyouma Achille, Mbo Nkoulou Luther Fort, et al. Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization. Int J Genet Genomics. 2023;11(1):1-5. doi: 10.11648/j.ijgg.20231101.11

Copy | Download

@article{10.11648/j.ijgg.20231101.11,
  author = {Molo Thierry and Likeng-Li-Ngue Benoit Constant and Ngalle Hermine Bille and Nyouma Achille and Mbo Nkoulou Luther Fort and Eya’a Ngombo Clement and Bell Joseph Martin},
  title = {Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization},
  journal = {International Journal of Genetics and Genomics},
  volume = {11},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ijgg.20231101.11},
  url = {https://doi.org/10.11648/j.ijgg.20231101.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20231101.11},
  abstract = {The introduction of transgenic varieties of cotton (Gossypium hirsitum) in Cameroon has posed numerous concerns. One of the most concern was the horizontal transfer of the Bt gene to related species such as Abelmoschus esculentus. The current study was conducted to assess the occurrence Bt gene transfer from Gossypium hirsitum to Abelmoschus esculentus. To achieve this, the stigma receptivity periods and the viability of pollen grains were evaluated. The fruit set rates of 675 controlled intra- and interspecific crosses were assessed. The results showed that stigma receptivity and pollen grain viability are optimal at anthesis in both species, with a longer duration over 24 hours in G. hirsitum. Intra-specific crosses within the two species have shown fruit set rates of nearly 60-80%. Interspecific crosses gave a fruit set rate close to 2%, especially when A. esculentus is used as the female parent, and zero results in reciprocal crosses. The finding of the study pointed out that the possibility of horizontal transfer of a transgene to cotton relatives. In future studies, it would be useful to monitor the fate of seeds from interspecific crosses between both species and assess the agro-morphological and molecular characteristics of these possible hybrids.},
 year = {2023}
}

Copy | Download

TY - JOUR
T1 - Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization
AU - Molo Thierry
AU - Likeng-Li-Ngue Benoit Constant
AU - Ngalle Hermine Bille
AU - Nyouma Achille
AU - Mbo Nkoulou Luther Fort
AU - Eya’a Ngombo Clement
AU - Bell Joseph Martin
Y1 - 2023/02/24
PY - 2023
N1 - https://doi.org/10.11648/j.ijgg.20231101.11
DO - 10.11648/j.ijgg.20231101.11
T2 - International Journal of Genetics and Genomics
JF - International Journal of Genetics and Genomics
JO - International Journal of Genetics and Genomics
SP - 1
EP - 5
PB - Science Publishing Group
SN - 2376-7359
UR - https://doi.org/10.11648/j.ijgg.20231101.11
AB - The introduction of transgenic varieties of cotton (Gossypium hirsitum) in Cameroon has posed numerous concerns. One of the most concern was the horizontal transfer of the Bt gene to related species such as Abelmoschus esculentus. The current study was conducted to assess the occurrence Bt gene transfer from Gossypium hirsitum to Abelmoschus esculentus. To achieve this, the stigma receptivity periods and the viability of pollen grains were evaluated. The fruit set rates of 675 controlled intra- and interspecific crosses were assessed. The results showed that stigma receptivity and pollen grain viability are optimal at anthesis in both species, with a longer duration over 24 hours in G. hirsitum. Intra-specific crosses within the two species have shown fruit set rates of nearly 60-80%. Interspecific crosses gave a fruit set rate close to 2%, especially when A. esculentus is used as the female parent, and zero results in reciprocal crosses. The finding of the study pointed out that the possibility of horizontal transfer of a transgene to cotton relatives. In future studies, it would be useful to monitor the fate of seeds from interspecific crosses between both species and assess the agro-morphological and molecular characteristics of these possible hybrids.
VL - 11
IS - 1
ER -

Copy | Download

Author Information

Molo Thierry

Genetic and Plant Improvement Unit, Department of Plant Biology, Faculty of Science, University of Yaounde I, Yaounde I, Cameroon
Likeng-Li-Ngue Benoit Constant

Genetic and Plant Improvement Unit, Department of Plant Biology, Faculty of Science, University of Yaounde I, Yaounde I, Cameroon
Ngalle Hermine Bille

Genetic and Plant Improvement Unit, Department of Plant Biology, Faculty of Science, University of Yaounde I, Yaounde I, Cameroon
Nyouma Achille

Plant Biology and Physiology Laboratory, Department of Botany, Faculty of Science, University of Douala, Douala, Cameroon
Mbo Nkoulou Luther Fort

Plant Production Unit, Mbalmayo Agricultural Research Centre (CRAM), Institute of Agricultural Research for Development, Mbalmayo, Cameroon
Eya’a Ngombo Clement

Lipids Analysis Laboratory, Institute of Agricultural Research for Development, Specialized Station for Oil Palm of La Dibamba, Douala, Cameroon
Bell Joseph Martin

Genetic and Plant Improvement Unit, Department of Plant Biology, Faculty of Science, University of Yaounde I, Yaounde I, Cameroon

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Molo Thierry, Likeng-Li-Ngue Benoit Constant, Ngalle Hermine Bille, Nyouma Achille, Mbo Nkoulou Luther Fort, et al. (2023). Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization. International Journal of Genetics and Genomics, 11(1), 1-5. https://doi.org/10.11648/j.ijgg.20231101.11

Copy | Download

ACS Style

Molo Thierry; Likeng-Li-Ngue Benoit Constant; Ngalle Hermine Bille; Nyouma Achille; Mbo Nkoulou Luther Fort, et al. Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization. Int. J. Genet. Genomics 2023, 11(1), 1-5. doi: 10.11648/j.ijgg.20231101.11

Copy | Download

AMA Style

Molo Thierry, Likeng-Li-Ngue Benoit Constant, Ngalle Hermine Bille, Nyouma Achille, Mbo Nkoulou Luther Fort, et al. Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization. Int J Genet Genomics. 2023;11(1):1-5. doi: 10.11648/j.ijgg.20231101.11

Copy | Download

@article{10.11648/j.ijgg.20231101.11,
  author = {Molo Thierry and Likeng-Li-Ngue Benoit Constant and Ngalle Hermine Bille and Nyouma Achille and Mbo Nkoulou Luther Fort and Eya’a Ngombo Clement and Bell Joseph Martin},
  title = {Potential Gene Flow Between Gossypium hirsitum and Abelmoschus esculentus Through Interspecific Hybridization},
  journal = {International Journal of Genetics and Genomics},
  volume = {11},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ijgg.20231101.11},
  url = {https://doi.org/10.11648/j.ijgg.20231101.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20231101.11},
  abstract = {The introduction of transgenic varieties of cotton (Gossypium hirsitum) in Cameroon has posed numerous concerns. One of the most concern was the horizontal transfer of the Bt gene to related species such as Abelmoschus esculentus. The current study was conducted to assess the occurrence Bt gene transfer from Gossypium hirsitum to Abelmoschus esculentus. To achieve this, the stigma receptivity periods and the viability of pollen grains were evaluated. The fruit set rates of 675 controlled intra- and interspecific crosses were assessed. The results showed that stigma receptivity and pollen grain viability are optimal at anthesis in both species, with a longer duration over 24 hours in G. hirsitum. Intra-specific crosses within the two species have shown fruit set rates of nearly 60-80%. Interspecific crosses gave a fruit set rate close to 2%, especially when A. esculentus is used as the female parent, and zero results in reciprocal crosses. The finding of the study pointed out that the possibility of horizontal transfer of a transgene to cotton relatives. In future studies, it would be useful to monitor the fate of seeds from interspecific crosses between both species and assess the agro-morphological and molecular characteristics of these possible hybrids.},
 year = {2023}
}

Copy | Download